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The map label for this gene is mltB [H]

Identifier: 126174804

GI number: 126174804

Start: 3035773

End: 3037008

Strand: Reverse

Name: mltB [H]

Synonym: Sbal_2591

Alternate gene names: 126174804

Gene position: 3037008-3035773 (Counterclockwise)

Preceding gene: 126174807

Following gene: 126174803

Centisome position: 59.23

GC content: 50.08

Gene sequence:

>1236_bases
GTGCCAGCGGCGAGTGCCAGTCCCAGTACTAACGCAACCAGTAGTAACGCAAGTGGCATAGTCGCTGAAGCCAACCCAAT
GCCCGATATCAGTGCGAGCGTACAAGCAAATGCCGAGGCATTTCCGAGTTGTGTGACTGGACTACAAGCGCGGGCGCGCA
CTGAAGGGCTGTCAGAGGCGACGATTAACTCGACCATTGCAAATTTGAAATTTGTGCCTCGGGTGATTGAGCTCGATCAG
CAACAACCTGAATTCAGCCAGACATTCGATAATTATTTCAGTAAGCGCGCCACCGAATGGCGCGTCAATGAAGGCCGTCG
TTTACTGCAAAAGCACAGCGTATTACTCAATCAATTAGCCAAAGAATATGGCGTGCCACCGCAATATATCTTGTCTTTTT
GGGGGCTTGAAACTAACTACGGTTCCTATAAAGGCAAGATGTCGGTATTGAATTCGCTTGCGACTTTGGCCTGTGAGCCA
CGCCGTAGCAATTATTTCACCATAGAATTGATGCAAGCCTTAAAGCTGAAAGAAAAGTATCAGTTTGATGATGACACTAT
GGTGGGATCATGGGCTGGTGCTATGGGACACACGCAATTTATGCCGTCGGCCTATGCCAAATATGCCGTCGACGGTGACG
GCGATGGTAAAGCCGATTTATGGAATAGCACCACTGATGCGCTGACTTCCGCCGCGAACTTTTTACAGCACTTAGGCTGG
CAGCGAAATGAACGCTGGGGCCGTGAAGTCTTATTGCCAAAGGATTTTAGTTACAGCCATTTAGGTGCAAAGCAGCCCTT
GGCGTTATCGGAATGGGCTGCGCTCAATGTCACGCAAGCGAATGGTCAACCTTTGCCTGCCGTTGATATTCAAGCCGCGC
TGTATTTACCTGCAGGCCACACAGGGCCTGCCTTTTTAGGCTATGAGAACTTCAATGTGATCATGCGTTGGAATCGCTCT
GAGTATTATGCCATTACAGTGGGGCATCTCGCCGATCGCATTAATGGTGGCTTACCTTTGACAGTGAGCCCGCCAGAACA
GCCACGCTTAAGTCGCGAGCAAGTTAAGCAACTGCAAGCAAAACTGAATGAGGCCGGATTTGATGTTGGCAAGCCAGATG
GTGTGTTGGGGCGTAACTCTGTGGCAGGGCTGCAAGCCTTTCAACGAAGCAAACAGATGATTGCCGATGGCTTCCCAAGC
CCAGAGGTGTTTAGCGCTTTAGGGTTATCACTGTAG

Upstream 100 bases:

>100_bases
ATATTGGAGTTGTTGATTTGATTAAAATTAAAGGGTTAACTTATCTGTTTGCGAGTATTGCCGCCTGTGTCAGTCTGAGT
GCCTGTTCCAGCGTTCCAGC

Downstream 100 bases:

>100_bases
TCACTGTAGCCACTGTCACTGTAGTCACTGTAGCCAAAGAGCGCTCGCTGCGGGTTTTGAGCGCTTTTAGGCCGGATAAA
AAAGTGGAGTGAAAAGCTCA

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MPAASASPSTNATSSNASGIVAEANPMPDISASVQANAEAFPSCVTGLQARARTEGLSEATINSTIANLKFVPRVIELDQ
QQPEFSQTFDNYFSKRATEWRVNEGRRLLQKHSVLLNQLAKEYGVPPQYILSFWGLETNYGSYKGKMSVLNSLATLACEP
RRSNYFTIELMQALKLKEKYQFDDDTMVGSWAGAMGHTQFMPSAYAKYAVDGDGDGKADLWNSTTDALTSAANFLQHLGW
QRNERWGREVLLPKDFSYSHLGAKQPLALSEWAALNVTQANGQPLPAVDIQAALYLPAGHTGPAFLGYENFNVIMRWNRS
EYYAITVGHLADRINGGLPLTVSPPEQPRLSREQVKQLQAKLNEAGFDVGKPDGVLGRNSVAGLQAFQRSKQMIADGFPS
PEVFSALGLSL

Sequences:

>Translated_411_residues
MPAASASPSTNATSSNASGIVAEANPMPDISASVQANAEAFPSCVTGLQARARTEGLSEATINSTIANLKFVPRVIELDQ
QQPEFSQTFDNYFSKRATEWRVNEGRRLLQKHSVLLNQLAKEYGVPPQYILSFWGLETNYGSYKGKMSVLNSLATLACEP
RRSNYFTIELMQALKLKEKYQFDDDTMVGSWAGAMGHTQFMPSAYAKYAVDGDGDGKADLWNSTTDALTSAANFLQHLGW
QRNERWGREVLLPKDFSYSHLGAKQPLALSEWAALNVTQANGQPLPAVDIQAALYLPAGHTGPAFLGYENFNVIMRWNRS
EYYAITVGHLADRINGGLPLTVSPPEQPRLSREQVKQLQAKLNEAGFDVGKPDGVLGRNSVAGLQAFQRSKQMIADGFPS
PEVFSALGLSL
>Mature_410_residues
PAASASPSTNATSSNASGIVAEANPMPDISASVQANAEAFPSCVTGLQARARTEGLSEATINSTIANLKFVPRVIELDQQ
QPEFSQTFDNYFSKRATEWRVNEGRRLLQKHSVLLNQLAKEYGVPPQYILSFWGLETNYGSYKGKMSVLNSLATLACEPR
RSNYFTIELMQALKLKEKYQFDDDTMVGSWAGAMGHTQFMPSAYAKYAVDGDGDGKADLWNSTTDALTSAANFLQHLGWQ
RNERWGREVLLPKDFSYSHLGAKQPLALSEWAALNVTQANGQPLPAVDIQAALYLPAGHTGPAFLGYENFNVIMRWNRSE
YYAITVGHLADRINGGLPLTVSPPEQPRLSREQVKQLQAKLNEAGFDVGKPDGVLGRNSVAGLQAFQRSKQMIADGFPSP
EVFSALGLSL

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=249, Percent_Identity=35.7429718875502, Blast_Score=128, Evalue=7e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 44990; Mature: 44859

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAASASPSTNATSSNASGIVAEANPMPDISASVQANAEAFPSCVTGLQARARTEGLSEA
CCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHH
TINSTIANLKFVPRVIELDQQQPEFSQTFDNYFSKRATEWRVNEGRRLLQKHSVLLNQLA
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
KEYGVPPQYILSFWGLETNYGSYKGKMSVLNSLATLACEPRRSNYFTIELMQALKLKEKY
HHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHC
QFDDDTMVGSWAGAMGHTQFMPSAYAKYAVDGDGDGKADLWNSTTDALTSAANFLQHLGW
CCCCCCEEHHHHCCCCCHHHCCHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
QRNERWGREVLLPKDFSYSHLGAKQPLALSEWAALNVTQANGQPLPAVDIQAALYLPAGH
CCCCCCCCEEECCCCCCCHHCCCCCCCCHHCCCEEEEECCCCCCCCCCCCEEEEEECCCC
TGPAFLGYENFNVIMRWNRSEYYAITVGHLADRINGGLPLTVSPPEQPRLSREQVKQLQA
CCCEEEEECCCEEEEEECCCCEEEEEHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHH
KLNEAGFDVGKPDGVLGRNSVAGLQAFQRSKQMIADGFPSPEVFSALGLSL
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCC
>Mature Secondary Structure 
PAASASPSTNATSSNASGIVAEANPMPDISASVQANAEAFPSCVTGLQARARTEGLSEA
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHH
TINSTIANLKFVPRVIELDQQQPEFSQTFDNYFSKRATEWRVNEGRRLLQKHSVLLNQLA
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
KEYGVPPQYILSFWGLETNYGSYKGKMSVLNSLATLACEPRRSNYFTIELMQALKLKEKY
HHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHC
QFDDDTMVGSWAGAMGHTQFMPSAYAKYAVDGDGDGKADLWNSTTDALTSAANFLQHLGW
CCCCCCEEHHHHCCCCCHHHCCHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
QRNERWGREVLLPKDFSYSHLGAKQPLALSEWAALNVTQANGQPLPAVDIQAALYLPAGH
CCCCCCCCEEECCCCCCCHHCCCCCCCCHHCCCEEEEECCCCCCCCCCCCEEEEEECCCC
TGPAFLGYENFNVIMRWNRSEYYAITVGHLADRINGGLPLTVSPPEQPRLSREQVKQLQA
CCCEEEEECCCEEEEEECCCCEEEEEHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHH
KLNEAGFDVGKPDGVLGRNSVAGLQAFQRSKQMIADGFPSPEVFSALGLSL
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]