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The map label for this gene is ushA [H]

Identifier: 126174796

GI number: 126174796

Start: 3027990

End: 3029708

Strand: Reverse

Name: ushA [H]

Synonym: Sbal_2583

Alternate gene names: 126174796

Gene position: 3029708-3027990 (Counterclockwise)

Preceding gene: 126174797

Following gene: 126174793

Centisome position: 59.09

GC content: 44.39

Gene sequence:

>1719_bases
ATGAGACATATGCTTATTAAAGGACTAGTTGCAACTGCTGTTGTCGCTGCGCTTGCGGGTTGTAATAATAGTGATGACGA
TAAAGTCCCAACAACATGTGCTGAAGCGGGAAGCGCTTGCACAACATTTACCGTTTTACACACCAATGACAACCATGGCC
GCTTCTGGGAAAACAGTGACGGTGAATATGGTATGGCAGCACGTAAAACCTTAATTGACGGTATTCGTGCAGAAGTCAGT
AAAAACGGTGGTCAAACATTACTGCTGTCAGGCGGTGATATTAATACTGGCGTACCAGAATCGGATCTACAAGATGCGAT
CCCAGATTTCACGGGTATGAATAAAATTGGTTACGACGCTATGGCTGTCGGTAACCATGAGTTTGATAATCCATTATCCG
TTGTTGATATGCAGCGCCGTCTTGCAGAATTCCCTATGTTAGCGGCGAACATTTACCGTAGAGATGTGGATGGCAATCCG
GGGGAACGTTACTTTGATGCCTATAAAGTATTTGATGTAAACGGTCTAAAAGTCGCGGTAATTGGCTTAACGACCGAAGA
TACGGCTAAGATTGGTAATCCAGAATTCATTAGTGGCCTGAAGTTCACCGATCCAAAGACTGAAGTCGCTAAAGTGATTA
AAGAAATTAAAGACGCCAAAGCGGCCGATATTATTTTCGCTACCACGCACATGGGTCATTATGCTGACGGCCATAACGGT
AGTAATGCCCCTGGTGATGTGGCTATGGCTCGCGCACTGAAAGAAGGCGATTTGCAAGTGGTTATTGGTGGTCATTCACA
AAACCCAGTGTGTATGGAACCAGGTACTGACAACAAAGCTTACGCTGACTTCAAACCAGGCGATGAGTGTGCTCCAGATA
AACAAAATGGTACTTGGATCATGCAAGCCCATGAATGGGGTAAATATGTTGGCCGTGCAGATTTTGAATACTTTAACGGT
GAACTTCATTTAGCAAGCTACAAGCTTGTGCCTGTGAATATGCGTAAATTGGATGACGCAGGCAAGAAAACAGCTGATTT
AGCTGGTACTAAGATTGAACCTGATGCTGAGTTAAAAGAGCTGCTGTCTTACTACCAAGAGAAAGGTCAAGCTAAGTTAG
ACGAAGTCATAGCAACAACGGATGCCCTGTTAGATGGCGAGCGTGCAAACGTGCGTAACAAGCAAACTAACTTAGGCCGT
ATGTTAGCGATGGCACAAAGTGATAAAGTCAAAGCCGATTTTGGCGTGATGAACTCGGGCGGTGTACGTGCCTCTATTCA
GCCAGGCAATATCACTTACCGTGACGTGCTGACTGTTCAGCCATTCGGTAACATGGTGACTCTGAACGAAATGACAGGCA
CTGAAGTTGCGGCTTATTTAGGCGCAGTAGCTAGTCTTCAAATTGGTTCTGGTGGTTATGCACAAATCACGGGCGTGAAA
ATGACCATTGACTGTACGGCTAAGACTGCCAATATCAGTGTGATTAATGGTGAGAACTTCAGCCCAACAGGAACTTATAA
GTTCACTGTACCTAGCTTTAACGCTGCAGGTGGTGACGGTTATCCTAAGCTAGAAAGCCCAATTCAAACGGGTTTTGTTG
ATGCTGATTTACTCTATTCTTTCTTGAAAGAGAAACAAGCGATTAAAGCTGCTGACTACAGTCCGATAGGCGATATCGTT
TATGAAAACTCAAACAGTGTTGAAGGTTGTCAACTTTAA

Upstream 100 bases:

>100_bases
AATTTATCTTTATTCTTTGTTATTATCCGTGACGCTGAACTTGATGGAGTTCACGGCAATCTTTCTATGATAAAATAATT
AAAATCAAGGGGTTGATGTA

Downstream 100 bases:

>100_bases
GTAATCAATCATTACTCAGTGTTTAAATATAAATGCCACTCAATTGAGTGGCATTTTTTATATGACAGTTTCTATCATGT
TTAAAGAATACTTAGTTCAT

Product: bifunctional UDP-sugar hydrolase/5'-nucleotidase periplasmic precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MRHMLIKGLVATAVVAALAGCNNSDDDKVPTTCAEAGSACTTFTVLHTNDNHGRFWENSDGEYGMAARKTLIDGIRAEVS
KNGGQTLLLSGGDINTGVPESDLQDAIPDFTGMNKIGYDAMAVGNHEFDNPLSVVDMQRRLAEFPMLAANIYRRDVDGNP
GERYFDAYKVFDVNGLKVAVIGLTTEDTAKIGNPEFISGLKFTDPKTEVAKVIKEIKDAKAADIIFATTHMGHYADGHNG
SNAPGDVAMARALKEGDLQVVIGGHSQNPVCMEPGTDNKAYADFKPGDECAPDKQNGTWIMQAHEWGKYVGRADFEYFNG
ELHLASYKLVPVNMRKLDDAGKKTADLAGTKIEPDAELKELLSYYQEKGQAKLDEVIATTDALLDGERANVRNKQTNLGR
MLAMAQSDKVKADFGVMNSGGVRASIQPGNITYRDVLTVQPFGNMVTLNEMTGTEVAAYLGAVASLQIGSGGYAQITGVK
MTIDCTAKTANISVINGENFSPTGTYKFTVPSFNAAGGDGYPKLESPIQTGFVDADLLYSFLKEKQAIKAADYSPIGDIV
YENSNSVEGCQL

Sequences:

>Translated_572_residues
MRHMLIKGLVATAVVAALAGCNNSDDDKVPTTCAEAGSACTTFTVLHTNDNHGRFWENSDGEYGMAARKTLIDGIRAEVS
KNGGQTLLLSGGDINTGVPESDLQDAIPDFTGMNKIGYDAMAVGNHEFDNPLSVVDMQRRLAEFPMLAANIYRRDVDGNP
GERYFDAYKVFDVNGLKVAVIGLTTEDTAKIGNPEFISGLKFTDPKTEVAKVIKEIKDAKAADIIFATTHMGHYADGHNG
SNAPGDVAMARALKEGDLQVVIGGHSQNPVCMEPGTDNKAYADFKPGDECAPDKQNGTWIMQAHEWGKYVGRADFEYFNG
ELHLASYKLVPVNMRKLDDAGKKTADLAGTKIEPDAELKELLSYYQEKGQAKLDEVIATTDALLDGERANVRNKQTNLGR
MLAMAQSDKVKADFGVMNSGGVRASIQPGNITYRDVLTVQPFGNMVTLNEMTGTEVAAYLGAVASLQIGSGGYAQITGVK
MTIDCTAKTANISVINGENFSPTGTYKFTVPSFNAAGGDGYPKLESPIQTGFVDADLLYSFLKEKQAIKAADYSPIGDIV
YENSNSVEGCQL
>Mature_572_residues
MRHMLIKGLVATAVVAALAGCNNSDDDKVPTTCAEAGSACTTFTVLHTNDNHGRFWENSDGEYGMAARKTLIDGIRAEVS
KNGGQTLLLSGGDINTGVPESDLQDAIPDFTGMNKIGYDAMAVGNHEFDNPLSVVDMQRRLAEFPMLAANIYRRDVDGNP
GERYFDAYKVFDVNGLKVAVIGLTTEDTAKIGNPEFISGLKFTDPKTEVAKVIKEIKDAKAADIIFATTHMGHYADGHNG
SNAPGDVAMARALKEGDLQVVIGGHSQNPVCMEPGTDNKAYADFKPGDECAPDKQNGTWIMQAHEWGKYVGRADFEYFNG
ELHLASYKLVPVNMRKLDDAGKKTADLAGTKIEPDAELKELLSYYQEKGQAKLDEVIATTDALLDGERANVRNKQTNLGR
MLAMAQSDKVKADFGVMNSGGVRASIQPGNITYRDVLTVQPFGNMVTLNEMTGTEVAAYLGAVASLQIGSGGYAQITGVK
MTIDCTAKTANISVINGENFSPTGTYKFTVPSFNAAGGDGYPKLESPIQTGFVDADLLYSFLKEKQAIKAADYSPIGDIV
YENSNSVEGCQL

Specific function: Degradation of extracellular 5'-nucleotides for nutritional needs [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=544, Percent_Identity=23.7132352941176, Blast_Score=111, Evalue=2e-24,
Organism=Escherichia coli, GI1786687, Length=539, Percent_Identity=50.834879406308, Blast_Score=559, Evalue=1e-160,
Organism=Escherichia coli, GI1790658, Length=527, Percent_Identity=20.8728652751423, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI19922444, Length=572, Percent_Identity=25.6993006993007, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI19922446, Length=575, Percent_Identity=25.0434782608696, Blast_Score=129, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24654424, Length=575, Percent_Identity=25.0434782608696, Blast_Score=129, Evalue=6e-30,
Organism=Drosophila melanogaster, GI28573524, Length=556, Percent_Identity=24.1007194244604, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24641187, Length=462, Percent_Identity=24.8917748917749, Blast_Score=96, Evalue=9e-20,
Organism=Drosophila melanogaster, GI161076508, Length=443, Percent_Identity=23.9277652370203, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19921980, Length=443, Percent_Identity=23.9277652370203, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24652512, Length=443, Percent_Identity=23.9277652370203, Blast_Score=87, Evalue=4e-17,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.5 [H]

Molecular weight: Translated: 61465; Mature: 61465

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00962 RIBOSOMAL_S2_1 ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHMLIKGLVATAVVAALAGCNNSDDDKVPTTCAEAGSACTTFTVLHTNDNHGRFWENSD
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCEEEEEEEEECCCCCCCCCCCC
GEYGMAARKTLIDGIRAEVSKNGGQTLLLSGGDINTGVPESDLQDAIPDFTGMNKIGYDA
CCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHCCCCCCCCCCCCCE
MAVGNHEFDNPLSVVDMQRRLAEFPMLAANIYRRDVDGNPGERYFDAYKVFDVNGLKVAV
EEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHCCEEEEEECCCCEEEE
IGLTTEDTAKIGNPEFISGLKFTDPKTEVAKVIKEIKDAKAADIIFATTHMGHYADGHNG
EEECCCCHHCCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCC
SNAPGDVAMARALKEGDLQVVIGGHSQNPVCMEPGTDNKAYADFKPGDECAPDKQNGTWI
CCCCHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCCCCEEECCCCCCCCCCCCCCCEEE
MQAHEWGKYVGRADFEYFNGELHLASYKLVPVNMRKLDDAGKKTADLAGTKIEPDAELKE
EEECHHHHHHCCCCCEEECCEEEEEEEEEEECHHHHHHHCCCCHHHCCCCCCCCCHHHHH
LLSYYQEKGQAKLDEVIATTDALLDGERANVRNKQTNLGRMLAMAQSDKVKADFGVMNSG
HHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEECCCEECCC
GVRASIQPGNITYRDVLTVQPFGNMVTLNEMTGTEVAAYLGAVASLQIGSGGYAQITGVK
CEEEEECCCCEEEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHEEEECCCCEEEEEEEE
MTIDCTAKTANISVINGENFSPTGTYKFTVPSFNAAGGDGYPKLESPIQTGFVDADLLYS
EEEEEEEEEEEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHCCCCHHHHHH
FLKEKQAIKAADYSPIGDIVYENSNSVEGCQL
HHHHHHHHHHCCCCCCCCEEECCCCCCCCCCC
>Mature Secondary Structure
MRHMLIKGLVATAVVAALAGCNNSDDDKVPTTCAEAGSACTTFTVLHTNDNHGRFWENSD
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCEEEEEEEEECCCCCCCCCCCC
GEYGMAARKTLIDGIRAEVSKNGGQTLLLSGGDINTGVPESDLQDAIPDFTGMNKIGYDA
CCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHCCCCCCCCCCCCCE
MAVGNHEFDNPLSVVDMQRRLAEFPMLAANIYRRDVDGNPGERYFDAYKVFDVNGLKVAV
EEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHCCEEEEEECCCCEEEE
IGLTTEDTAKIGNPEFISGLKFTDPKTEVAKVIKEIKDAKAADIIFATTHMGHYADGHNG
EEECCCCHHCCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCC
SNAPGDVAMARALKEGDLQVVIGGHSQNPVCMEPGTDNKAYADFKPGDECAPDKQNGTWI
CCCCHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCCCCEEECCCCCCCCCCCCCCCEEE
MQAHEWGKYVGRADFEYFNGELHLASYKLVPVNMRKLDDAGKKTADLAGTKIEPDAELKE
EEECHHHHHHCCCCCEEECCEEEEEEEEEEECHHHHHHHCCCCHHHCCCCCCCCCHHHHH
LLSYYQEKGQAKLDEVIATTDALLDGERANVRNKQTNLGRMLAMAQSDKVKADFGVMNSG
HHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEECCCEECCC
GVRASIQPGNITYRDVLTVQPFGNMVTLNEMTGTEVAAYLGAVASLQIGSGGYAQITGVK
CEEEEECCCCEEEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHEEEECCCCEEEEEEEE
MTIDCTAKTANISVINGENFSPTGTYKFTVPSFNAAGGDGYPKLESPIQTGFVDADLLYS
EEEEEEEEEEEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHCCCCHHHHHH
FLKEKQAIKAADYSPIGDIVYENSNSVEGCQL
HHHHHHHHHHCCCCCCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA