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The map label for this gene is nadE

Identifier: 126174756

GI number: 126174756

Start: 2975374

End: 2976204

Strand: Reverse

Name: nadE

Synonym: Sbal_2543

Alternate gene names: 126174756

Gene position: 2976204-2975374 (Counterclockwise)

Preceding gene: 126174757

Following gene: 126174754

Centisome position: 58.05

GC content: 49.58

Gene sequence:

>831_bases
GTGAAAGCGCAAATCTTAAGAGAAATGAAGGTACTCACGGCCATAGAGCCAGAGTTCGAAGTGCAACGTCGGGTCGCGTT
TATTAAAACCAAACTAAAAGAAGCGCGCAGCAAGGCATTGGTATTAGGGATCAGCGGTGGGGTGGATTCTTCTACCGCTG
GGCGTTTGTGTCAGTTGGCGGTGGACAGTTTAAACCACGAAAATAGCCAAGGCGGTTACCAGTTTATCGCCGTGCGCTTA
CCCTATCAGATCCAAAAAGATGAGCATGAGGCGCAATTAGCCTGTCAGTTTATTCAGCCCTCTAAATTGGTCACAGTGAA
TGTTCATCAAGGTGTCGATGGCGTGCACAGCGCTACCGTTGCCGCGTTAGCCGAAGCTGGATTGCCTTTGCCCGATGTGG
CAAAAGTGGATTTTGTTAAGGGTAACGTTAAAGCTCGTATGCGGATGATCGCTCAATACGAACTCGCCGGACTCGTAGGT
GGACTCGTCGTGGGGACAGATCACAGCGCCGAAAACATCACAGGCTTTTACACTAAGTGGGGCGATGGTGCCTGCGATTT
AGCGCCGTTATTTGGTCTTAACAAGCGCCAAGTTCGCCAACTTGCCGCGTATCTTGGTGCGCCAGAGTCTTTAGTGCATA
AAGCGCCGACCGCGGATCTAGAAGACAATAAGCCGCTACTCGAAGATGAAGTCGCACTTGGGTTAACTTATGCGCAAATC
GATGATTTCCTTGAGGGTAAAGATGTCGGCAAGGCAGTTGAAGACAAGTTGATTGGTATTTATAAGGCGACGCAGCATAA
ACGTCAGCCTATCCCAACGATCTACGACTAA

Upstream 100 bases:

>100_bases
GCCAAGCGCTAATTTTAACTACAGGCAGTTGCGAATAAATGTTAACGTTAGTCGGGTTCAGATAGTCCAAATACAGCGAT
TATTTAAGGTGGAATTTTCG

Downstream 100 bases:

>100_bases
CGCTAAATCGTACTCGAATAAGCACTCGTTTGAACTCAGTTCACGAGTGCTTTTTTATGCCTTTTCCTTACCTAGTTGCT
GAGCTTGCGGCAGCCTTTAC

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MKAQILREMKVLTAIEPEFEVQRRVAFIKTKLKEARSKALVLGISGGVDSSTAGRLCQLAVDSLNHENSQGGYQFIAVRL
PYQIQKDEHEAQLACQFIQPSKLVTVNVHQGVDGVHSATVAALAEAGLPLPDVAKVDFVKGNVKARMRMIAQYELAGLVG
GLVVGTDHSAENITGFYTKWGDGACDLAPLFGLNKRQVRQLAAYLGAPESLVHKAPTADLEDNKPLLEDEVALGLTYAQI
DDFLEGKDVGKAVEDKLIGIYKATQHKRQPIPTIYD

Sequences:

>Translated_276_residues
MKAQILREMKVLTAIEPEFEVQRRVAFIKTKLKEARSKALVLGISGGVDSSTAGRLCQLAVDSLNHENSQGGYQFIAVRL
PYQIQKDEHEAQLACQFIQPSKLVTVNVHQGVDGVHSATVAALAEAGLPLPDVAKVDFVKGNVKARMRMIAQYELAGLVG
GLVVGTDHSAENITGFYTKWGDGACDLAPLFGLNKRQVRQLAAYLGAPESLVHKAPTADLEDNKPLLEDEVALGLTYAQI
DDFLEGKDVGKAVEDKLIGIYKATQHKRQPIPTIYD
>Mature_276_residues
MKAQILREMKVLTAIEPEFEVQRRVAFIKTKLKEARSKALVLGISGGVDSSTAGRLCQLAVDSLNHENSQGGYQFIAVRL
PYQIQKDEHEAQLACQFIQPSKLVTVNVHQGVDGVHSATVAALAEAGLPLPDVAKVDFVKGNVKARMRMIAQYELAGLVG
GLVVGTDHSAENITGFYTKWGDGACDLAPLFGLNKRQVRQLAAYLGAPESLVHKAPTADLEDNKPLLEDEVALGLTYAQI
DDFLEGKDVGKAVEDKLIGIYKATQHKRQPIPTIYD

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family

Homologues:

Organism=Escherichia coli, GI1788036, Length=277, Percent_Identity=51.2635379061372, Blast_Score=268, Evalue=4e-73,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NADE_SHEB2 (B8E735)

Other databases:

- EMBL:   CP001252
- RefSeq:   YP_002357731.1
- ProteinModelPortal:   B8E735
- SMR:   B8E735
- GeneID:   7088337
- GenomeReviews:   CP001252_GR
- KEGG:   sbp:Sbal223_1803
- HOGENOM:   HBG351567
- OMA:   KVAQATI
- ProtClustDB:   PRK00768
- HAMAP:   MF_00193
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00552

Pfam domain/function: PF02540 NAD_synthase

EC number: =6.3.1.5

Molecular weight: Translated: 30017; Mature: 30017

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: ACT_SITE 45-45

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAQILREMKVLTAIEPEFEVQRRVAFIKTKLKEARSKALVLGISGGVDSSTAGRLCQLA
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHH
VDSLNHENSQGGYQFIAVRLPYQIQKDEHEAQLACQFIQPSKLVTVNVHQGVDGVHSATV
HHHCCCCCCCCCEEEEEEECCCEECCCHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHH
AALAEAGLPLPDVAKVDFVKGNVKARMRMIAQYELAGLVGGLVVGTDHSAENITGFYTKW
HHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCEEEEEEC
GDGACDLAPLFGLNKRQVRQLAAYLGAPESLVHKAPTADLEDNKPLLEDEVALGLTYAQI
CCCCCCHHHHCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHHHHEEHHHHH
DDFLEGKDVGKAVEDKLIGIYKATQHKRQPIPTIYD
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKAQILREMKVLTAIEPEFEVQRRVAFIKTKLKEARSKALVLGISGGVDSSTAGRLCQLA
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHH
VDSLNHENSQGGYQFIAVRLPYQIQKDEHEAQLACQFIQPSKLVTVNVHQGVDGVHSATV
HHHCCCCCCCCCEEEEEEECCCEECCCHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHH
AALAEAGLPLPDVAKVDFVKGNVKARMRMIAQYELAGLVGGLVVGTDHSAENITGFYTKW
HHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCEEEEEEC
GDGACDLAPLFGLNKRQVRQLAAYLGAPESLVHKAPTADLEDNKPLLEDEVALGLTYAQI
CCCCCCHHHHCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHHHHEEHHHHH
DDFLEGKDVGKAVEDKLIGIYKATQHKRQPIPTIYD
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA