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The map label for this gene is ycdT [H]

Identifier: 126174753

GI number: 126174753

Start: 2970496

End: 2972730

Strand: Direct

Name: ycdT [H]

Synonym: Sbal_2540

Alternate gene names: 126174753

Gene position: 2970496-2972730 (Clockwise)

Preceding gene: 126174751

Following gene: 126174755

Centisome position: 57.93

GC content: 44.12

Gene sequence:

>2235_bases
TTGTGCCATAATCACAGACAGTTAACACAAGCAAATTTTCAAAGGGACTTTTCTTTGCATTTACCCGCCTTCCAAACCGC
CCATCTCAGACTGGCAATTATTCTGGGTGTATTCGGCTTAGTCCTGAATCTTTTTCCCATCCCTTTATTCGCTAATGTGC
AATTGATTCTCGGCAACGTGGCAGTTGTGATAGTGGCAATACTCTTAGGGCCTTGGTATGCATTGATCACCGCACTATTT
ACCGCAACGGGACTCATGATCGTGTGGTCGAGCCCGCACGTGTATTTACTGTTTTTACTCGAAGCATTATGGCTTGGTTT
TGCAAGGCGCCGAGATATTCAAATACTCTATGCCAGTGTGAGTTATTGGGTATTATTGGGGATCCCGCTGTTAGCTATTT
ATGTGGCTGTCATTGCTAAAATGCCCGCCAGCCATATTCCTTTTACTGCGATAAAACAAGCCGTTAATGGCATGATTTAC
GCTGCAATTGGTGAACTCTGTGTGGTTGCTATTCCAAGCCTATGGCATTTTAAAGGCAAGCTGACCAATCTTAACCGGCG
CACTTTTAGCTCGCAGCTCAGTTACTTGTTTACCTTAATCATTACTGTGAGTTTATTAGTTTCGTCCTTAGCCTTTAATC
ACTTTTTCATTGATAAACAACAGGTGTTGATTAATCGCAATCTGGACGATACGGCAACCCATTTGAGCCATGCAACCGAC
AACTATCTCGCTTACAACACCCAAGTCATCGCTAGCACAGCCAAGTTTTTGAGCCTGTCGAATGCAGATATCAATGAGTG
GCAGGCATTACTGTCGAGCGTACACGATAGTAATCAAGGCTTTAAAACCATGTTACTAGCTAATGAGCAAGGCAACTTAC
TCGCTGCCAGTCCGATGGCCAATATAGTAAAATTAGACAGTCTCTCTGACATTTCTTCGGTGAGCGATAGGGAATACTTT
ATCCAAGCGTTTTACAACCATAAAACCTTTGTATCACCTGCGTTTATCGGCAGAGGTTTTGGTAATGACGTGATCGTTGC
CATCAGTGCCCCCATTTTTAGTCCAAATGATCCCAATCAAGCCAGAGGGATAGTCGAAGGCAGCTTAGATTTACGCTATT
TTTCCAGCATAGATAAACAAAACCTGCACCATGAGCAGCAATCTATTTTACTGACAGATGAGAACAATAATCTGATTTAT
GCCTCCGAAGGGTTAGGACTAGCACCGCTCACCCCCTTGTCATTCAGCAAAGGCAGTGAGATTTATCGCGCCCGGCTTCA
ACTGATGAACCTGCATAACCTAGATTCAAATACTCCTGAGTACATCTATGCCCAGCATAAGTTAAACAATGGTTGGCAAC
TCTATGTTTTAGAACCTTTTGTGCCGCTTCTAAAACTGGCTGAGCGCCAATATGTCAACACGGTAATTTTACTGTTTTGC
TCCTTAGTCGGTGCCTTTTTTATCACTAAAGCCATCAGTAAGTTACTGACCGAACCTCTGTCGCTTTTAGCGCAGCATTT
CGGTCCCGCTAAGCAAGAAAAAGCCAGCGACGAGAAGTTTGAACATGATCTACTCGATAAAAGCACCCCTAAAGAAATCT
ATAGCTTGTATGAAAGTTTAGCCTCGAACCAACAAGCCTTACTCGAACACCAACAAGAACTTGAACAAAAAGTACAGCAG
CGCACCCAAGATTTAGAAGCCGCTAATGTGAAATTAAAGGATTTAGCCGAGCGCGATCCGCTGACGAATCTCTATAATCG
CCGCTATACCGAGCATCAATTTCCACATATCCAACAGATGTGCGAACGGGGTCAAGATGCCATGACGCTTGCCATATTGG
ATTTAGACCATTTTAAGCAAATCAATGATACTTATGGCCATTTGGGCGGCGACGAGTGTTTAAAAGTGGTTGCTGAACTC
TTAAGCTCGCTCTTCAAGCGAGATATTGATTTGATCTCCCGCTACGGCGGCGAGGAATTTTTGCTGATTTTACCTATGTG
TAATGCCTTGAAAGTCGAAGCACATCTCAATGAGTTTAAACGGCAGCTCGCAGGCACTGTGATCATCAATCCGCAGGATC
ATCGAAGCTTCAAGGTCACGGCGAGTATCGGCGCTGTGATTGCCAATGCCACTTATAGCGACTCCTTAGAGTACTGGCTT
AAACAGGCCGACAATAACTTGTATCTGGCAAAGGAACAGGGACGTGATCGGGTTGTTTGCAGCCTGATTGTTTAA

Upstream 100 bases:

>100_bases
CTTTAATCCGTTCCCATAATCTGTTCCTACAATTCATTTCTATTATGGCTTTATATTGGGGGCTTCAATTGAAGCTCTGG
GATAGAGGCATTGGGTTTGT

Downstream 100 bases:

>100_bases
CTGGAACTTGGTTAAGAGAAAGCCGCTGAGGAGACAGTCGTTCAAAGGTAAAGAAGATTTAGGTCAGATTAACTGAGTGG
CATTCAATCACTATTAGATT

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 744; Mature: 744

Protein sequence:

>744_residues
MCHNHRQLTQANFQRDFSLHLPAFQTAHLRLAIILGVFGLVLNLFPIPLFANVQLILGNVAVVIVAILLGPWYALITALF
TATGLMIVWSSPHVYLLFLLEALWLGFARRRDIQILYASVSYWVLLGIPLLAIYVAVIAKMPASHIPFTAIKQAVNGMIY
AAIGELCVVAIPSLWHFKGKLTNLNRRTFSSQLSYLFTLIITVSLLVSSLAFNHFFIDKQQVLINRNLDDTATHLSHATD
NYLAYNTQVIASTAKFLSLSNADINEWQALLSSVHDSNQGFKTMLLANEQGNLLAASPMANIVKLDSLSDISSVSDREYF
IQAFYNHKTFVSPAFIGRGFGNDVIVAISAPIFSPNDPNQARGIVEGSLDLRYFSSIDKQNLHHEQQSILLTDENNNLIY
ASEGLGLAPLTPLSFSKGSEIYRARLQLMNLHNLDSNTPEYIYAQHKLNNGWQLYVLEPFVPLLKLAERQYVNTVILLFC
SLVGAFFITKAISKLLTEPLSLLAQHFGPAKQEKASDEKFEHDLLDKSTPKEIYSLYESLASNQQALLEHQQELEQKVQQ
RTQDLEAANVKLKDLAERDPLTNLYNRRYTEHQFPHIQQMCERGQDAMTLAILDLDHFKQINDTYGHLGGDECLKVVAEL
LSSLFKRDIDLISRYGGEEFLLILPMCNALKVEAHLNEFKRQLAGTVIINPQDHRSFKVTASIGAVIANATYSDSLEYWL
KQADNNLYLAKEQGRDRVVCSLIV

Sequences:

>Translated_744_residues
MCHNHRQLTQANFQRDFSLHLPAFQTAHLRLAIILGVFGLVLNLFPIPLFANVQLILGNVAVVIVAILLGPWYALITALF
TATGLMIVWSSPHVYLLFLLEALWLGFARRRDIQILYASVSYWVLLGIPLLAIYVAVIAKMPASHIPFTAIKQAVNGMIY
AAIGELCVVAIPSLWHFKGKLTNLNRRTFSSQLSYLFTLIITVSLLVSSLAFNHFFIDKQQVLINRNLDDTATHLSHATD
NYLAYNTQVIASTAKFLSLSNADINEWQALLSSVHDSNQGFKTMLLANEQGNLLAASPMANIVKLDSLSDISSVSDREYF
IQAFYNHKTFVSPAFIGRGFGNDVIVAISAPIFSPNDPNQARGIVEGSLDLRYFSSIDKQNLHHEQQSILLTDENNNLIY
ASEGLGLAPLTPLSFSKGSEIYRARLQLMNLHNLDSNTPEYIYAQHKLNNGWQLYVLEPFVPLLKLAERQYVNTVILLFC
SLVGAFFITKAISKLLTEPLSLLAQHFGPAKQEKASDEKFEHDLLDKSTPKEIYSLYESLASNQQALLEHQQELEQKVQQ
RTQDLEAANVKLKDLAERDPLTNLYNRRYTEHQFPHIQQMCERGQDAMTLAILDLDHFKQINDTYGHLGGDECLKVVAEL
LSSLFKRDIDLISRYGGEEFLLILPMCNALKVEAHLNEFKRQLAGTVIINPQDHRSFKVTASIGAVIANATYSDSLEYWL
KQADNNLYLAKEQGRDRVVCSLIV
>Mature_744_residues
MCHNHRQLTQANFQRDFSLHLPAFQTAHLRLAIILGVFGLVLNLFPIPLFANVQLILGNVAVVIVAILLGPWYALITALF
TATGLMIVWSSPHVYLLFLLEALWLGFARRRDIQILYASVSYWVLLGIPLLAIYVAVIAKMPASHIPFTAIKQAVNGMIY
AAIGELCVVAIPSLWHFKGKLTNLNRRTFSSQLSYLFTLIITVSLLVSSLAFNHFFIDKQQVLINRNLDDTATHLSHATD
NYLAYNTQVIASTAKFLSLSNADINEWQALLSSVHDSNQGFKTMLLANEQGNLLAASPMANIVKLDSLSDISSVSDREYF
IQAFYNHKTFVSPAFIGRGFGNDVIVAISAPIFSPNDPNQARGIVEGSLDLRYFSSIDKQNLHHEQQSILLTDENNNLIY
ASEGLGLAPLTPLSFSKGSEIYRARLQLMNLHNLDSNTPEYIYAQHKLNNGWQLYVLEPFVPLLKLAERQYVNTVILLFC
SLVGAFFITKAISKLLTEPLSLLAQHFGPAKQEKASDEKFEHDLLDKSTPKEIYSLYESLASNQQALLEHQQELEQKVQQ
RTQDLEAANVKLKDLAERDPLTNLYNRRYTEHQFPHIQQMCERGQDAMTLAILDLDHFKQINDTYGHLGGDECLKVVAEL
LSSLFKRDIDLISRYGGEEFLLILPMCNALKVEAHLNEFKRQLAGTVIINPQDHRSFKVTASIGAVIANATYSDSLEYWL
KQADNNLYLAKEQGRDRVVCSLIV

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=172, Percent_Identity=36.046511627907, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1786584, Length=178, Percent_Identity=33.7078651685393, Blast_Score=96, Evalue=8e-21,
Organism=Escherichia coli, GI87082007, Length=173, Percent_Identity=35.8381502890173, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI87081881, Length=175, Percent_Identity=36.5714285714286, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788381, Length=185, Percent_Identity=33.5135135135135, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI145693134, Length=167, Percent_Identity=32.9341317365269, Blast_Score=81, Evalue=3e-16,
Organism=Escherichia coli, GI1787816, Length=161, Percent_Identity=32.9192546583851, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1787802, Length=183, Percent_Identity=32.2404371584699, Blast_Score=80, Evalue=7e-16,
Organism=Escherichia coli, GI1787541, Length=168, Percent_Identity=30.3571428571429, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1787056, Length=178, Percent_Identity=29.2134831460674, Blast_Score=64, Evalue=4e-11,
Organism=Escherichia coli, GI87081974, Length=161, Percent_Identity=31.6770186335404, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 83665; Mature: 83665

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCHNHRQLTQANFQRDFSLHLPAFQTAHLRLAIILGVFGLVLNLFPIPLFANVQLILGNV
CCCCCHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
AVVIVAILLGPWYALITALFTATGLMIVWSSPHVYLLFLLEALWLGFARRRDIQILYASV
HHHHHHHHHCCHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEHHH
SYWVLLGIPLLAIYVAVIAKMPASHIPFTAIKQAVNGMIYAAIGELCVVAIPSLWHFKGK
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCH
LTNLNRRTFSSQLSYLFTLIITVSLLVSSLAFNHFFIDKQQVLINRNLDDTATHLSHATD
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHEEECCCCHHHHHHHHCCC
NYLAYNTQVIASTAKFLSLSNADINEWQALLSSVHDSNQGFKTMLLANEQGNLLAASPMA
CEEEECHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEECCHH
NIVKLDSLSDISSVSDREYFIQAFYNHKTFVSPAFIGRGFGNDVIVAISAPIFSPNDPNQ
HHHHHCCCHHHHHCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEECCCCCCCCCCH
ARGIVEGSLDLRYFSSIDKQNLHHEQQSILLTDENNNLIYASEGLGLAPLTPLSFSKGSE
HCCEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCHH
IYRARLQLMNLHNLDSNTPEYIYAQHKLNNGWQLYVLEPFVPLLKLAERQYVNTVILLFC
HHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
SLVGAFFITKAISKLLTEPLSLLAQHFGPAKQEKASDEKFEHDLLDKSTPKEIYSLYESL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHCCCCHHHHHHHHHHH
ASNQQALLEHQQELEQKVQQRTQDLEAANVKLKDLAERDPLTNLYNRRYTEHQFPHIQQM
HCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHCCCCCCCCHHHHHH
CERGQDAMTLAILDLDHFKQINDTYGHLGGDECLKVVAELLSSLFKRDIDLISRYGGEEF
HHCCCCEEEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LLILPMCNALKVEAHLNEFKRQLAGTVIINPQDHRSFKVTASIGAVIANATYSDSLEYWL
EEEEECCCHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEEEHHHHHCCCCCHHHHHHHH
KQADNNLYLAKEQGRDRVVCSLIV
HHCCCCEEEECCCCCCEEEEEEEC
>Mature Secondary Structure
MCHNHRQLTQANFQRDFSLHLPAFQTAHLRLAIILGVFGLVLNLFPIPLFANVQLILGNV
CCCCCHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
AVVIVAILLGPWYALITALFTATGLMIVWSSPHVYLLFLLEALWLGFARRRDIQILYASV
HHHHHHHHHCCHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEHHH
SYWVLLGIPLLAIYVAVIAKMPASHIPFTAIKQAVNGMIYAAIGELCVVAIPSLWHFKGK
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCH
LTNLNRRTFSSQLSYLFTLIITVSLLVSSLAFNHFFIDKQQVLINRNLDDTATHLSHATD
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHEEECCCCHHHHHHHHCCC
NYLAYNTQVIASTAKFLSLSNADINEWQALLSSVHDSNQGFKTMLLANEQGNLLAASPMA
CEEEECHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEECCHH
NIVKLDSLSDISSVSDREYFIQAFYNHKTFVSPAFIGRGFGNDVIVAISAPIFSPNDPNQ
HHHHHCCCHHHHHCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEECCCCCCCCCCH
ARGIVEGSLDLRYFSSIDKQNLHHEQQSILLTDENNNLIYASEGLGLAPLTPLSFSKGSE
HCCEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCHH
IYRARLQLMNLHNLDSNTPEYIYAQHKLNNGWQLYVLEPFVPLLKLAERQYVNTVILLFC
HHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
SLVGAFFITKAISKLLTEPLSLLAQHFGPAKQEKASDEKFEHDLLDKSTPKEIYSLYESL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHCCCCHHHHHHHHHHH
ASNQQALLEHQQELEQKVQQRTQDLEAANVKLKDLAERDPLTNLYNRRYTEHQFPHIQQM
HCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHCCCCCCCCHHHHHH
CERGQDAMTLAILDLDHFKQINDTYGHLGGDECLKVVAELLSSLFKRDIDLISRYGGEEF
HHCCCCEEEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LLILPMCNALKVEAHLNEFKRQLAGTVIINPQDHRSFKVTASIGAVIANATYSDSLEYWL
EEEEECCCHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEEEHHHHHCCCCCHHHHHHHH
KQADNNLYLAKEQGRDRVVCSLIV
HHCCCCEEEECCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]