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The map label for this gene is cysL [H]

Identifier: 126174751

GI number: 126174751

Start: 2968018

End: 2968884

Strand: Direct

Name: cysL [H]

Synonym: Sbal_2538

Alternate gene names: 126174751

Gene position: 2968018-2968884 (Clockwise)

Preceding gene: 126174749

Following gene: 126174753

Centisome position: 57.89

GC content: 48.1

Gene sequence:

>867_bases
ATGATAAATCCACAGTGGTTACACACCTTTGCTGTTCTGGTCGAGAAAGGTAACTTCACCCGAACCGCCGAAGCCATTGG
TATCACTCAAGCGGCTGTGAGCCAACATATCAGTCGCTTAGAAGAGGAATACGGCACACTGTTTTTACGCCGCCCCCGTC
AACTTGAAATCACTCCGATTGGCGAAAACCTGCTGACCTACGTTAAAGACGTCGAATGCGCGCAAAAACGCCTACAGCAG
AAACTGATCCATGATGATGCTGAGGTCGGCGACATTAGCCTTATCACTCCGGGGAGCATTGGTTTAACACTCTTTCCGCT
GTTAATCGAACTTCAACAAGCCTTTCCGGCGTTAAACATTCGCCACCGTTTTGCCCCTGACAGTGAAATAGTCGCCTCGG
TATTAGCCAATGAACATGAAATCGGCATGGTGACCTTAAAACCTTCCGATCCTCGCATTCATAGCACGCATTTCACCGAA
GAGCCGCTTGAGCTCGTCGTACCTGCGTGCCACAAACAAAATGAATGGGTTGATTTACAACGGCTAGGGTTTATCTCCCA
TCCCGATGGCGAGGCCATGGCAACTCGGTTACTTAGCCGAAAATATCCAAGTAATCCCGGTATTCAAGACATTCCCTGTA
AAGGATTTACCAACCAAATCAGCCTGATCCTCGAACCTGTCGCGAAAGGACTAGGATTTACAGTTATACCGCAATACGCC
CGCAAAGCCTTTGAACGACAACAAGATATCTATGTTGTGGAATGTGGCAGCCAAGTTGTAGACACCATTTGGCTTATCCA
CCGCGCGGAATGGCCCTTGTCACGCAGAGCCCAAAAAGCGATTACATTTTTAAAGGGGAAGATATAA

Upstream 100 bases:

>100_bases
ATAGAATCAAAAAGGAATGGAATACTGAATGACGACAGACTAGCCTTATCGTGTCATAATTAAAAGCACGCACATATTAT
AGAGTTGATAAAGTTTGCTT

Downstream 100 bases:

>100_bases
AAGCCTTATATTACTCTCCTCCTTGTATGGATAAAGTCCTTAAAAACCCAAGCCAGTGTCAGTGTCGCAATTTAAAGCAA
CTCATTAAAAACGCGATGCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MINPQWLHTFAVLVEKGNFTRTAEAIGITQAAVSQHISRLEEEYGTLFLRRPRQLEITPIGENLLTYVKDVECAQKRLQQ
KLIHDDAEVGDISLITPGSIGLTLFPLLIELQQAFPALNIRHRFAPDSEIVASVLANEHEIGMVTLKPSDPRIHSTHFTE
EPLELVVPACHKQNEWVDLQRLGFISHPDGEAMATRLLSRKYPSNPGIQDIPCKGFTNQISLILEPVAKGLGFTVIPQYA
RKAFERQQDIYVVECGSQVVDTIWLIHRAEWPLSRRAQKAITFLKGKI

Sequences:

>Translated_288_residues
MINPQWLHTFAVLVEKGNFTRTAEAIGITQAAVSQHISRLEEEYGTLFLRRPRQLEITPIGENLLTYVKDVECAQKRLQQ
KLIHDDAEVGDISLITPGSIGLTLFPLLIELQQAFPALNIRHRFAPDSEIVASVLANEHEIGMVTLKPSDPRIHSTHFTE
EPLELVVPACHKQNEWVDLQRLGFISHPDGEAMATRLLSRKYPSNPGIQDIPCKGFTNQISLILEPVAKGLGFTVIPQYA
RKAFERQQDIYVVECGSQVVDTIWLIHRAEWPLSRRAQKAITFLKGKI
>Mature_288_residues
MINPQWLHTFAVLVEKGNFTRTAEAIGITQAAVSQHISRLEEEYGTLFLRRPRQLEITPIGENLLTYVKDVECAQKRLQQ
KLIHDDAEVGDISLITPGSIGLTLFPLLIELQQAFPALNIRHRFAPDSEIVASVLANEHEIGMVTLKPSDPRIHSTHFTE
EPLELVVPACHKQNEWVDLQRLGFISHPDGEAMATRLLSRKYPSNPGIQDIPCKGFTNQISLILEPVAKGLGFTVIPQYA
RKAFERQQDIYVVECGSQVVDTIWLIHRAEWPLSRRAQKAITFLKGKI

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=187, Percent_Identity=28.8770053475936, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32496; Mature: 32496

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINPQWLHTFAVLVEKGNFTRTAEAIGITQAAVSQHISRLEEEYGTLFLRRPRQLEITPI
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEEEEEC
GENLLTYVKDVECAQKRLQQKLIHDDAEVGDISLITPGSIGLTLFPLLIELQQAFPALNI
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCC
RHRFAPDSEIVASVLANEHEIGMVTLKPSDPRIHSTHFTEEPLELVVPACHKQNEWVDLQ
HHCCCCCHHHHHHHHCCCCCEEEEEECCCCCCEECCCCCCCCHHHHHHHHCCCCCCCCHH
RLGFISHPDGEAMATRLLSRKYPSNPGIQDIPCKGFTNQISLILEPVAKGLGFTVIPQYA
HCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEHHHHH
RKAFERQQDIYVVECGSQVVDTIWLIHRAEWPLSRRAQKAITFLKGKI
HHHHHHCCCEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MINPQWLHTFAVLVEKGNFTRTAEAIGITQAAVSQHISRLEEEYGTLFLRRPRQLEITPI
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEEEEEC
GENLLTYVKDVECAQKRLQQKLIHDDAEVGDISLITPGSIGLTLFPLLIELQQAFPALNI
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCC
RHRFAPDSEIVASVLANEHEIGMVTLKPSDPRIHSTHFTEEPLELVVPACHKQNEWVDLQ
HHCCCCCHHHHHHHHCCCCCEEEEEECCCCCCEECCCCCCCCHHHHHHHHCCCCCCCCHH
RLGFISHPDGEAMATRLLSRKYPSNPGIQDIPCKGFTNQISLILEPVAKGLGFTVIPQYA
HCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEHHHHH
RKAFERQQDIYVVECGSQVVDTIWLIHRAEWPLSRRAQKAITFLKGKI
HHHHHHCCCEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]