Click here to switch to the map view.

The map label for this gene is purB [H]

Identifier: 126174693

GI number: 126174693

Start: 2893474

End: 2894844

Strand: Direct

Name: purB [H]

Synonym: Sbal_2480

Alternate gene names: 126174693

Gene position: 2893474-2894844 (Clockwise)

Preceding gene: 126174692

Following gene: 126174694

Centisome position: 56.43

GC content: 46.1

Gene sequence:

>1371_bases
ATGGATCTTTCCGCACTAACTGCTATCTCTCCGGTAGACGGTCGTTATGGTAGTAAAACCGCGTCATTGAGAGGGATTTT
CAGCGAGTTCGGTCTTACCAAGTACCGTGTTCAGGTTGAAATCAATTGGCTAAAACTGTTAGCAAACTGCCCAGACATTA
GCGAAGTGCCAACATTGAGCGCGTCAGCAATCGCTGTGCTCGATGGCATTAAAGACAATTTCAACGAACAAGATGCGCTG
CGTGTTAAGGCTATCGAAAGCACCACTAACCACGATGTGAAAGCGGTTGAATACTTCATCAAAGAAAAAATCGCGGGCAA
TAGCGAATTAGCTGCTGTGGGTGAGTTTGTTCACTTTGCCTGTACTTCTGAAGATATCAACAACTTAAGCCACGGTTTAA
TGTTGACTGAAGCCCGTGAACAAGTATTACTGCCCTACTGCAACGAGTTGCTGACTGCGATTAAAAAGCTGGCGATTGAA
TATCGATCTGTGCCATTAATGTCACGCACCCACGGCCAACCTGCTTCACCTTCAACACTTGGTAAAGAGATGGCGAACGT
AGCGATGCGTCTTGAGCGTCAAATCAAACAAATCGCCAATGTTGAAATCATGGGCAAACTGAACGGTGCCGTAGGTAACT
ACAATGCTCACCTGTCAGCCTATCCTGAAGTTAACTGGCATGAACTGTCTGAGCGTTTTGTGACTAGCCTTGGCCTGCAC
TGGAATGCCTACACAACGCAAATCGAACCACACGATTACATTGCTGAACTGTTTGATGCGGTTGCCCGTTTCAACACAGT
GCTGATTGACTTTGATCGTGACATTTGGGGTTACATTGCCCTAGGTCACTTCAAACAACGCACTATCGCCGGTGAAATCG
GTTCATCGACTATGCCACACAAAGTTAACCCAATCGATTTTGAAAACTCTGAGGGTAACTTAGGTATCGCTAACGCGCTG
ATGCAACATTTAGCGTCTAAGCTGCCGTTATCAAGATGGCAACGTGACTTAACTGACTCAACTGTGCTGCGTAACTTAGG
CGTAGGTATTGCCCATGGGTTGATCGCGTATCAAGCGACCTTAAAAGGCATCAGCAAGTTAGAAGTTAACGAAGCGCACC
TGCGTAATGAGTTAGATCACAACTGGGAAGTGTTAGCAGAACCCGTACAAACGGTAATGCGTCGTTATGGTATTGAAAAG
CCATACGAAAAGTTAAAAGAACTGACGCGCGGTAAACGTATCGACGCGCAGCAACTGTCAGTGTTTATTGACGGACTTGA
ATTACCAGACTCAGTGAAAGCTGAACTGAAGAAAATGACGCCCGCCAATTACATTGGTCGCGCCGAAACGTTTGTCGACG
AACTGAAGTAA

Upstream 100 bases:

>100_bases
GTAAACGTCGACACTTAGTCTTCGCCCGCAAAGCCATAGTCGATACAGCCAAGAAAAGTCTTACCCTATAATAAAATTAG
CGTTCATCAAGGAGCTTCAC

Downstream 100 bases:

>100_bases
GCTTTTAACTGAACTTAGTTTTTAACTGAAATTAGTTATTAGCCGAAATTGGCGATGGACAATACTCTTTCATAAACAGA
AATAAGGATTAAGGCGACTC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MDLSALTAISPVDGRYGSKTASLRGIFSEFGLTKYRVQVEINWLKLLANCPDISEVPTLSASAIAVLDGIKDNFNEQDAL
RVKAIESTTNHDVKAVEYFIKEKIAGNSELAAVGEFVHFACTSEDINNLSHGLMLTEAREQVLLPYCNELLTAIKKLAIE
YRSVPLMSRTHGQPASPSTLGKEMANVAMRLERQIKQIANVEIMGKLNGAVGNYNAHLSAYPEVNWHELSERFVTSLGLH
WNAYTTQIEPHDYIAELFDAVARFNTVLIDFDRDIWGYIALGHFKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGIANAL
MQHLASKLPLSRWQRDLTDSTVLRNLGVGIAHGLIAYQATLKGISKLEVNEAHLRNELDHNWEVLAEPVQTVMRRYGIEK
PYEKLKELTRGKRIDAQQLSVFIDGLELPDSVKAELKKMTPANYIGRAETFVDELK

Sequences:

>Translated_456_residues
MDLSALTAISPVDGRYGSKTASLRGIFSEFGLTKYRVQVEINWLKLLANCPDISEVPTLSASAIAVLDGIKDNFNEQDAL
RVKAIESTTNHDVKAVEYFIKEKIAGNSELAAVGEFVHFACTSEDINNLSHGLMLTEAREQVLLPYCNELLTAIKKLAIE
YRSVPLMSRTHGQPASPSTLGKEMANVAMRLERQIKQIANVEIMGKLNGAVGNYNAHLSAYPEVNWHELSERFVTSLGLH
WNAYTTQIEPHDYIAELFDAVARFNTVLIDFDRDIWGYIALGHFKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGIANAL
MQHLASKLPLSRWQRDLTDSTVLRNLGVGIAHGLIAYQATLKGISKLEVNEAHLRNELDHNWEVLAEPVQTVMRRYGIEK
PYEKLKELTRGKRIDAQQLSVFIDGLELPDSVKAELKKMTPANYIGRAETFVDELK
>Mature_456_residues
MDLSALTAISPVDGRYGSKTASLRGIFSEFGLTKYRVQVEINWLKLLANCPDISEVPTLSASAIAVLDGIKDNFNEQDAL
RVKAIESTTNHDVKAVEYFIKEKIAGNSELAAVGEFVHFACTSEDINNLSHGLMLTEAREQVLLPYCNELLTAIKKLAIE
YRSVPLMSRTHGQPASPSTLGKEMANVAMRLERQIKQIANVEIMGKLNGAVGNYNAHLSAYPEVNWHELSERFVTSLGLH
WNAYTTQIEPHDYIAELFDAVARFNTVLIDFDRDIWGYIALGHFKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGIANAL
MQHLASKLPLSRWQRDLTDSTVLRNLGVGIAHGLIAYQATLKGISKLEVNEAHLRNELDHNWEVLAEPVQTVMRRYGIEK
PYEKLKELTRGKRIDAQQLSVFIDGLELPDSVKAELKKMTPANYIGRAETFVDELK

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=456, Percent_Identity=74.780701754386, Blast_Score=739, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 50901; Mature: 50901

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLSALTAISPVDGRYGSKTASLRGIFSEFGLTKYRVQVEINWLKLLANCPDISEVPTLS
CCCCHHCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEEEHHHHHHHCCCCCCCCCCCC
ASAIAVLDGIKDNFNEQDALRVKAIESTTNHDVKAVEYFIKEKIAGNSELAAVGEFVHFA
HHHHHHHHHHHHCCCCCHHHEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
CTSEDINNLSHGLMLTEAREQVLLPYCNELLTAIKKLAIEYRSVPLMSRTHGQPASPSTL
CCHHHHHHHHHCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCHHHH
GKEMANVAMRLERQIKQIANVEIMGKLNGAVGNYNAHLSAYPEVNWHELSERFVTSLGLH
HHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCC
WNAYTTQIEPHDYIAELFDAVARFNTVLIDFDRDIWGYIALGHFKQRTIAGEIGSSTMPH
CCCEEECCCHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KVNPIDFENSEGNLGIANALMQHLASKLPLSRWQRDLTDSTVLRNLGVGIAHGLIAYQAT
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
LKGISKLEVNEAHLRNELDHNWEVLAEPVQTVMRRYGIEKPYEKLKELTRGKRIDAQQLS
HHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHH
VFIDGLELPDSVKAELKKMTPANYIGRAETFVDELK
HHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDLSALTAISPVDGRYGSKTASLRGIFSEFGLTKYRVQVEINWLKLLANCPDISEVPTLS
CCCCHHCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEEEHHHHHHHCCCCCCCCCCCC
ASAIAVLDGIKDNFNEQDALRVKAIESTTNHDVKAVEYFIKEKIAGNSELAAVGEFVHFA
HHHHHHHHHHHHCCCCCHHHEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
CTSEDINNLSHGLMLTEAREQVLLPYCNELLTAIKKLAIEYRSVPLMSRTHGQPASPSTL
CCHHHHHHHHHCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCHHHH
GKEMANVAMRLERQIKQIANVEIMGKLNGAVGNYNAHLSAYPEVNWHELSERFVTSLGLH
HHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCC
WNAYTTQIEPHDYIAELFDAVARFNTVLIDFDRDIWGYIALGHFKQRTIAGEIGSSTMPH
CCCEEECCCHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KVNPIDFENSEGNLGIANALMQHLASKLPLSRWQRDLTDSTVLRNLGVGIAHGLIAYQAT
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
LKGISKLEVNEAHLRNELDHNWEVLAEPVQTVMRRYGIEKPYEKLKELTRGKRIDAQQLS
HHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHH
VFIDGLELPDSVKAELKKMTPANYIGRAETFVDELK
HHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]