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The map label for this gene is rluE [H]

Identifier: 126174689

GI number: 126174689

Start: 2890043

End: 2890855

Strand: Direct

Name: rluE [H]

Synonym: Sbal_2476

Alternate gene names: 126174689

Gene position: 2890043-2890855 (Clockwise)

Preceding gene: 126174687

Following gene: 126174690

Centisome position: 56.36

GC content: 46.49

Gene sequence:

>813_bases
GTGTCTCAACCTCCTGTTAGCAAAAGTATGAACAAAAACAAGTTTCCACCTAAACGTGCCAATTCCAACGCCAGTTCCCC
AGCCAAGAGCACGATTAAACCCGCTAAGCCCGCCAGCAGAGGCCCTGCCAACCGCGCTACCGCCGTAGATAGCAAAGTGG
CGCCAGCACGTAATGCCAATGGCAGTTTAAAGAAAGAACCCTTTAAAAATCCTAATCGACGCGCAATTGCAGCGGCAAAA
CCAGTGCAGAGCCAAACGCTCGCACCGATTATCGTGCTGTTTAATAAGCCTTTTGATGTACTTTGCCAGTTTACCGATGA
ACAAGGTCGCAAAACTCTAAAAGACTATATTCCCATTGCTGACGTGTATGCCGCAGGCCGCTTAGACAGAGACAGTGAGG
GCTTGTTACTGCTGACCAATGACGGTCAATTACAGGCAAGACTGACAGAACCGAAAAAGAAAACTTTTAAAACCTATTGG
GTGCAAGTAGAAGGCGTGCCGACGGAAGATGCACTGACAAAACTGCGCCAAGGTGTTGAATTAAATGATGGTATGACGCT
GCCTGCTAAAGTCAAAATCATGGATTCACCCGATATTTGGCCACGTAATCCACCGGTGAGAGAACGCAAAAATATCCCAA
CAACTTGGTTAGAAATCCAAATTCATGAAGGCCGTAACCGCCAAGTCAGACGCATGACAGCGCATATCGGTTTTCCGACC
TTAAGATTAATCCGTTATAAAATAGGCCAATGGAGTATAGATAATTTGCCATCGGGCGAGCATAAATGTATCCGTCTCGG
TGATGAAGTCTAA

Upstream 100 bases:

>100_bases
GCCGACATCATAGAGCATTCGCAATAATATTCAAATTCCACTAACGGCGAATGCCAAGTACACTATGACCACATTAACGA
CTCAATCAAGTCAGGTAAGC

Downstream 100 bases:

>100_bases
ATTTCAGCCAAGTTTCTGCGCTCAACGCCACTAAAAATTGGCACCATGTCCACTCAAAGGAGGCACTATGAGTCAACGAT
ATAAACCCAATGTGACCGTC

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MSQPPVSKSMNKNKFPPKRANSNASSPAKSTIKPAKPASRGPANRATAVDSKVAPARNANGSLKKEPFKNPNRRAIAAAK
PVQSQTLAPIIVLFNKPFDVLCQFTDEQGRKTLKDYIPIADVYAAGRLDRDSEGLLLLTNDGQLQARLTEPKKKTFKTYW
VQVEGVPTEDALTKLRQGVELNDGMTLPAKVKIMDSPDIWPRNPPVRERKNIPTTWLEIQIHEGRNRQVRRMTAHIGFPT
LRLIRYKIGQWSIDNLPSGEHKCIRLGDEV

Sequences:

>Translated_270_residues
MSQPPVSKSMNKNKFPPKRANSNASSPAKSTIKPAKPASRGPANRATAVDSKVAPARNANGSLKKEPFKNPNRRAIAAAK
PVQSQTLAPIIVLFNKPFDVLCQFTDEQGRKTLKDYIPIADVYAAGRLDRDSEGLLLLTNDGQLQARLTEPKKKTFKTYW
VQVEGVPTEDALTKLRQGVELNDGMTLPAKVKIMDSPDIWPRNPPVRERKNIPTTWLEIQIHEGRNRQVRRMTAHIGFPT
LRLIRYKIGQWSIDNLPSGEHKCIRLGDEV
>Mature_269_residues
SQPPVSKSMNKNKFPPKRANSNASSPAKSTIKPAKPASRGPANRATAVDSKVAPARNANGSLKKEPFKNPNRRAIAAAKP
VQSQTLAPIIVLFNKPFDVLCQFTDEQGRKTLKDYIPIADVYAAGRLDRDSEGLLLLTNDGQLQARLTEPKKKTFKTYWV
QVEGVPTEDALTKLRQGVELNDGMTLPAKVKIMDSPDIWPRNPPVRERKNIPTTWLEIQIHEGRNRQVRRMTAHIGFPTL
RLIRYKIGQWSIDNLPSGEHKCIRLGDEV

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=175, Percent_Identity=69.1428571428571, Blast_Score=263, Evalue=7e-72,
Organism=Escherichia coli, GI1787524, Length=162, Percent_Identity=33.9506172839506, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788510, Length=185, Percent_Identity=32.4324324324324, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790453, Length=225, Percent_Identity=26.2222222222222, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 30187; Mature: 30056

Theoretical pI: Translated: 10.85; Mature: 10.85

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPPVSKSMNKNKFPPKRANSNASSPAKSTIKPAKPASRGPANRATAVDSKVAPARNAN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCCC
GSLKKEPFKNPNRRAIAAAKPVQSQTLAPIIVLFNKPFDVLCQFTDEQGRKTLKDYIPIA
CCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCHHHEEEECCHHHHHHHHHHCCHH
DVYAAGRLDRDSEGLLLLTNDGQLQARLTEPKKKTFKTYWVQVEGVPTEDALTKLRQGVE
HHHHHCCCCCCCCCEEEEECCCCEEEEECCCHHHCEEEEEEEEECCCCHHHHHHHHCCCC
LNDGMTLPAKVKIMDSPDIWPRNPPVRERKNIPTTWLEIQIHEGRNRQVRRMTAHIGFPT
CCCCCCCCEEEEEECCCCCCCCCCCCHHHCCCCCEEEEEEEECCCCCHHHHHHHHCCCHH
LRLIRYKIGQWSIDNLPSGEHKCIRLGDEV
HHHHHHHCCCEECCCCCCCCCEEEECCCCC
>Mature Secondary Structure 
SQPPVSKSMNKNKFPPKRANSNASSPAKSTIKPAKPASRGPANRATAVDSKVAPARNAN
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCCC
GSLKKEPFKNPNRRAIAAAKPVQSQTLAPIIVLFNKPFDVLCQFTDEQGRKTLKDYIPIA
CCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCHHHEEEECCHHHHHHHHHHCCHH
DVYAAGRLDRDSEGLLLLTNDGQLQARLTEPKKKTFKTYWVQVEGVPTEDALTKLRQGVE
HHHHHCCCCCCCCCEEEEECCCCEEEEECCCHHHCEEEEEEEEECCCCHHHHHHHHCCCC
LNDGMTLPAKVKIMDSPDIWPRNPPVRERKNIPTTWLEIQIHEGRNRQVRRMTAHIGFPT
CCCCCCCCEEEEEECCCCCCCCCCCCHHHCCCCCEEEEEEEECCCCCHHHHHHHHCCCHH
LRLIRYKIGQWSIDNLPSGEHKCIRLGDEV
HHHHHHHCCCEECCCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]