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The map label for this gene is metG [H]

Identifier: 126174678

GI number: 126174678

Start: 2877477

End: 2879546

Strand: Direct

Name: metG [H]

Synonym: Sbal_2465

Alternate gene names: 126174678

Gene position: 2877477-2879546 (Clockwise)

Preceding gene: 126174668

Following gene: 126174679

Centisome position: 56.12

GC content: 48.31

Gene sequence:

>2070_bases
ATGGCAACTTCACAACGTAAAATTCTCGTGACCAGCGCACTTCCCTACGCAAACGGACCGATACATTTAGGTCACATGTT
GGAATACATCCAGACAGATATCTGGTCGCGTTATCAAAAACTTCGTGGACATGAGTGCCACTATATTTGTGCCGATGACG
CCCATGGCACGCCGATCATGCTTAAAGCGCAGCAATTAGGCATAGCGCCTGAAGATATGATCGCCCAAGTGAACAAAGAA
CATCAGCAGGATTTTGCCGATTTTAATGTCGCCTTCGATAACTATCACAGCACTCACAGCGAAGAAAACCGCCTCATGGC
GAGCGATATTTATCTCAAGCTAAGGGACAACGGCTATATCAAGAGCAAGAGCATTTCTCAGTTATTCGATCCTGAGAAAT
CCATGTTCCTGCCCGACCGCTTCGTAAAAGGTACTTGCCCTAAGTGTAAGTCACCTGATCAATACGGCGATAACTGTGAT
TCTTGCGGCGCGACTTACAGCCCAACTGAACTGATCAATCCAAAATCAGCGGTATCGGGTGCAACGCCAGTGATGAAAGA
CACTGAGCACTTTTTCTTCGACCTGCCCGCCTTCGAAGGCATGCTAAAAGAGTGGACTCGCTCTGGCGCACTGCAAGTCG
AAATGGCCAATAAACTCGACGAATGGTTTGAGCAAGGTCTGCAGCAATGGGATATCACCCGTGATGCACCGTATTTTGGC
TTTGAAATTCCCGATGCACCGGGCAAGTATTTCTATGTTTGGTTAGATGCGCCTATCGGCTATATGGGTTCATTCAAAAA
CCTGTGTGCAAAACGTCCTGAGTTGAGCTTCGATGAATTCTGGGGCAAAGATTCTACTGCTGAGGTTTATCACTTCATCG
GTAAAGACATCGTTTACTTCCATAGCCTCTTCTGGCCAGCCATGCTGCACGGCTCAGGTTATCGTCAGCCAAACAGCGTC
TATGCCCACGGTTATGTGACGGTCAATGGCGCGAAGATGTCAAAATCGAAAGGCACGTTCATTAAGGCGCGTACCTATTT
AGATCATTTAGATCCTGAATATTTACGTTACTACTACGCCGCTAAACTCAGCAGCCGTATCGACGATTTAGATTTAAATC
TGGAAGATTTCGCCCAGCGCGTGAACTCAGATTTAGTCGGTAAACTAGTGAACTTAGCCTCGCGCACAGCAGGTTTTATC
ACTAAACGCTTCGATGGCAAGCTAGCGAAAATTAACGACACTACCCTGACCGAAGCGTTTTTAGCCAAGCAAGACGTGAT
TGCCGATTTTTACGAGTCGCGCGAATACGGCAAAGCGATGCGCGAAATCATGGCGCTGGCGGATATTGCCAACGGGTTTG
TGGCTGATGCTGCACCTTGGCAAATGGTGAAACATGACGATCAACAGGAAGCTGCCCATCAAGTGTGTTCAAACGCCTTG
AATCTGTTCCGTATTCTCGTGACGTATCTAAAACCTGTATTGCCACGTTTAGCCCAAGATGTGGAAGCCTTCTTCCAACT
GCCACTCACTTGGGATGCATTGGGCCAAGATTTAGCGGGTCATGAAATCGCCCCATTCAAGGCAATGATGCAACGTGTTG
AGCTGGATAAAGTCAATGCCATGGTTGCTGATTCCAAGGATAACCTGCAAGTCACGGCGGATGCACCAAAGACGGCAGCA
CCTGAAAAAATAGCTAAAGCTTCATCGGTTAGCAGTGAGCCGCTGGTTAGTGATCCTATCAGTGAGACCATCAACTTTGA
TGATTTTGCGAAAATCGACCTGCGTATCGCTCGCATCGTCAAAGCAGAGCATGTTGCTGACGCTGACAAGTTACTCAAGT
TACAACTCGACATCGGTGGTGAAACGCGCCAAGTGTTTGCCGGGATCAAATCGGCCTATTCGCCAGAAGATCTCGAAGGC
AAGTTGACTGTGATGGTGGCGAACTTAGCGCCACGTAAGATGCGTTTTGGCATGTCAGAAGGCATGGTGTTGGCCGCAGG
ACCTGGTGGTAGCGACCTGTGGATTTTAGAACCCCATGAAGGCGCACAGCCTGGCATGCGGGTTAAGTAA

Upstream 100 bases:

>100_bases
GCAGATTTAGCTCAATCGGCTCAGCTTTTGATGAAAATCATCCTGTAATCGGTTAGTATCTCTACCAATATTTTTGGCCC
ACCACGATATTTGAGACAAG

Downstream 100 bases:

>100_bases
TCTGCGCTAGCTAACGCTCGAAAAATCAATAAGGCCGCATTTATGTGGCCTTATTTGTTGGTTTTGACTGTTAGCCAATT
CGTGGCGCCTAGTGTCACTT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 689; Mature: 688

Protein sequence:

>689_residues
MATSQRKILVTSALPYANGPIHLGHMLEYIQTDIWSRYQKLRGHECHYICADDAHGTPIMLKAQQLGIAPEDMIAQVNKE
HQQDFADFNVAFDNYHSTHSEENRLMASDIYLKLRDNGYIKSKSISQLFDPEKSMFLPDRFVKGTCPKCKSPDQYGDNCD
SCGATYSPTELINPKSAVSGATPVMKDTEHFFFDLPAFEGMLKEWTRSGALQVEMANKLDEWFEQGLQQWDITRDAPYFG
FEIPDAPGKYFYVWLDAPIGYMGSFKNLCAKRPELSFDEFWGKDSTAEVYHFIGKDIVYFHSLFWPAMLHGSGYRQPNSV
YAHGYVTVNGAKMSKSKGTFIKARTYLDHLDPEYLRYYYAAKLSSRIDDLDLNLEDFAQRVNSDLVGKLVNLASRTAGFI
TKRFDGKLAKINDTTLTEAFLAKQDVIADFYESREYGKAMREIMALADIANGFVADAAPWQMVKHDDQQEAAHQVCSNAL
NLFRILVTYLKPVLPRLAQDVEAFFQLPLTWDALGQDLAGHEIAPFKAMMQRVELDKVNAMVADSKDNLQVTADAPKTAA
PEKIAKASSVSSEPLVSDPISETINFDDFAKIDLRIARIVKAEHVADADKLLKLQLDIGGETRQVFAGIKSAYSPEDLEG
KLTVMVANLAPRKMRFGMSEGMVLAAGPGGSDLWILEPHEGAQPGMRVK

Sequences:

>Translated_689_residues
MATSQRKILVTSALPYANGPIHLGHMLEYIQTDIWSRYQKLRGHECHYICADDAHGTPIMLKAQQLGIAPEDMIAQVNKE
HQQDFADFNVAFDNYHSTHSEENRLMASDIYLKLRDNGYIKSKSISQLFDPEKSMFLPDRFVKGTCPKCKSPDQYGDNCD
SCGATYSPTELINPKSAVSGATPVMKDTEHFFFDLPAFEGMLKEWTRSGALQVEMANKLDEWFEQGLQQWDITRDAPYFG
FEIPDAPGKYFYVWLDAPIGYMGSFKNLCAKRPELSFDEFWGKDSTAEVYHFIGKDIVYFHSLFWPAMLHGSGYRQPNSV
YAHGYVTVNGAKMSKSKGTFIKARTYLDHLDPEYLRYYYAAKLSSRIDDLDLNLEDFAQRVNSDLVGKLVNLASRTAGFI
TKRFDGKLAKINDTTLTEAFLAKQDVIADFYESREYGKAMREIMALADIANGFVADAAPWQMVKHDDQQEAAHQVCSNAL
NLFRILVTYLKPVLPRLAQDVEAFFQLPLTWDALGQDLAGHEIAPFKAMMQRVELDKVNAMVADSKDNLQVTADAPKTAA
PEKIAKASSVSSEPLVSDPISETINFDDFAKIDLRIARIVKAEHVADADKLLKLQLDIGGETRQVFAGIKSAYSPEDLEG
KLTVMVANLAPRKMRFGMSEGMVLAAGPGGSDLWILEPHEGAQPGMRVK
>Mature_688_residues
ATSQRKILVTSALPYANGPIHLGHMLEYIQTDIWSRYQKLRGHECHYICADDAHGTPIMLKAQQLGIAPEDMIAQVNKEH
QQDFADFNVAFDNYHSTHSEENRLMASDIYLKLRDNGYIKSKSISQLFDPEKSMFLPDRFVKGTCPKCKSPDQYGDNCDS
CGATYSPTELINPKSAVSGATPVMKDTEHFFFDLPAFEGMLKEWTRSGALQVEMANKLDEWFEQGLQQWDITRDAPYFGF
EIPDAPGKYFYVWLDAPIGYMGSFKNLCAKRPELSFDEFWGKDSTAEVYHFIGKDIVYFHSLFWPAMLHGSGYRQPNSVY
AHGYVTVNGAKMSKSKGTFIKARTYLDHLDPEYLRYYYAAKLSSRIDDLDLNLEDFAQRVNSDLVGKLVNLASRTAGFIT
KRFDGKLAKINDTTLTEAFLAKQDVIADFYESREYGKAMREIMALADIANGFVADAAPWQMVKHDDQQEAAHQVCSNALN
LFRILVTYLKPVLPRLAQDVEAFFQLPLTWDALGQDLAGHEIAPFKAMMQRVELDKVNAMVADSKDNLQVTADAPKTAAP
EKIAKASSVSSEPLVSDPISETINFDDFAKIDLRIARIVKAEHVADADKLLKLQLDIGGETRQVFAGIKSAYSPEDLEGK
LTVMVANLAPRKMRFGMSEGMVLAAGPGGSDLWILEPHEGAQPGMRVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=635, Percent_Identity=28.1889763779528, Blast_Score=256, Evalue=4e-68,
Organism=Homo sapiens, GI24308436, Length=535, Percent_Identity=24.8598130841122, Blast_Score=162, Evalue=1e-39,
Organism=Escherichia coli, GI1788432, Length=692, Percent_Identity=66.1849710982659, Blast_Score=983, Evalue=0.0,
Organism=Escherichia coli, GI1789455, Length=108, Percent_Identity=37.962962962963, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17540794, Length=668, Percent_Identity=27.0958083832335, Blast_Score=249, Evalue=3e-66,
Organism=Caenorhabditis elegans, GI212645096, Length=515, Percent_Identity=23.8834951456311, Blast_Score=144, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71992781, Length=410, Percent_Identity=24.1463414634146, Blast_Score=109, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6321704, Length=559, Percent_Identity=28.4436493738819, Blast_Score=249, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6321610, Length=543, Percent_Identity=23.9410681399632, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24655311, Length=582, Percent_Identity=29.3814432989691, Blast_Score=247, Evalue=2e-65,
Organism=Drosophila melanogaster, GI21358483, Length=535, Percent_Identity=23.5514018691589, Blast_Score=129, Evalue=6e-30,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 77282; Mature: 77150

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATSQRKILVTSALPYANGPIHLGHMLEYIQTDIWSRYQKLRGHECHYICADDAHGTPIM
CCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEE
LKAQQLGIAPEDMIAQVNKEHQQDFADFNVAFDNYHSTHSEENRLMASDIYLKLRDNGYI
EEHHHHCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCEEEEEEEEEECCCCCE
KSKSISQLFDPEKSMFLPDRFVKGTCPKCKSPDQYGDNCDSCGATYSPTELINPKSAVSG
ECCCHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCHHHCCCHHHCCC
ATPVMKDTEHFFFDLPAFEGMLKEWTRSGALQVEMANKLDEWFEQGLQQWDITRDAPYFG
CCCCCCCCCHHEEECHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCC
FEIPDAPGKYFYVWLDAPIGYMGSFKNLCAKRPELSFDEFWGKDSTAEVYHFIGKDIVYF
EECCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHH
HSLFWPAMLHGSGYRQPNSVYAHGYVTVNGAKMSKSKGTFIKARTYLDHLDPEYLRYYYA
HHHHHHHHHCCCCCCCCCCEEEEEEEEEECCEECCCCCCEEEEHHHHHHCCHHHHHHHHH
AKLSSRIDDLDLNLEDFAQRVNSDLVGKLVNLASRTAGFITKRFDGKLAKINDTTLTEAF
HHHHCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHH
LAKQDVIADFYESREYGKAMREIMALADIANGFVADAAPWQMVKHDDQQEAAHQVCSNAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHHH
NLFRILVTYLKPVLPRLAQDVEAFFQLPLTWDALGQDLAGHEIAPFKAMMQRVELDKVNA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHE
MVADSKDNLQVTADAPKTAAPEKIAKASSVSSEPLVSDPISETINFDDFAKIDLRIARIV
EEECCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHH
KAEHVADADKLLKLQLDIGGETRQVFAGIKSAYSPEDLEGKLTVMVANLAPRKMRFGMSE
HHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHCCCCC
GMVLAAGPGGSDLWILEPHEGAQPGMRVK
CEEEEECCCCCCEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
ATSQRKILVTSALPYANGPIHLGHMLEYIQTDIWSRYQKLRGHECHYICADDAHGTPIM
CCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEE
LKAQQLGIAPEDMIAQVNKEHQQDFADFNVAFDNYHSTHSEENRLMASDIYLKLRDNGYI
EEHHHHCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCEEEEEEEEEECCCCCE
KSKSISQLFDPEKSMFLPDRFVKGTCPKCKSPDQYGDNCDSCGATYSPTELINPKSAVSG
ECCCHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCHHHCCCHHHCCC
ATPVMKDTEHFFFDLPAFEGMLKEWTRSGALQVEMANKLDEWFEQGLQQWDITRDAPYFG
CCCCCCCCCHHEEECHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCC
FEIPDAPGKYFYVWLDAPIGYMGSFKNLCAKRPELSFDEFWGKDSTAEVYHFIGKDIVYF
EECCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHH
HSLFWPAMLHGSGYRQPNSVYAHGYVTVNGAKMSKSKGTFIKARTYLDHLDPEYLRYYYA
HHHHHHHHHCCCCCCCCCCEEEEEEEEEECCEECCCCCCEEEEHHHHHHCCHHHHHHHHH
AKLSSRIDDLDLNLEDFAQRVNSDLVGKLVNLASRTAGFITKRFDGKLAKINDTTLTEAF
HHHHCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHH
LAKQDVIADFYESREYGKAMREIMALADIANGFVADAAPWQMVKHDDQQEAAHQVCSNAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHHH
NLFRILVTYLKPVLPRLAQDVEAFFQLPLTWDALGQDLAGHEIAPFKAMMQRVELDKVNA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHE
MVADSKDNLQVTADAPKTAAPEKIAKASSVSSEPLVSDPISETINFDDFAKIDLRIARIV
EEECCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHH
KAEHVADADKLLKLQLDIGGETRQVFAGIKSAYSPEDLEGKLTVMVANLAPRKMRFGMSE
HHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHCCCCC
GMVLAAGPGGSDLWILEPHEGAQPGMRVK
CEEEEECCCCCCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA