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The map label for this gene is hisF [H]

Identifier: 126174644

GI number: 126174644

Start: 2832531

End: 2833304

Strand: Direct

Name: hisF [H]

Synonym: Sbal_2431

Alternate gene names: 126174644

Gene position: 2832531-2833304 (Clockwise)

Preceding gene: 126174643

Following gene: 126174645

Centisome position: 55.24

GC content: 51.68

Gene sequence:

>774_bases
ATGCTGGCCAAACGACTAGTCCCCTGTTTAGATGTGAAAGACGGTAAAGTAGTGAAAGGCGTGCAATTTCGTAACCACGA
AATTGTCGGTGATATCGTGCCCTTAGCCGCGCGTTATGCCGAAGAAGGCGCCGATGAGCTAGTGTTTTATGACATTACCG
CCAGCGCCCATGAACGTGTGGTCGATAAGTCGTGGGTGAGCCGCGTGGCGGAGCAGATTGATATTCCCTTCTGCGTGGCA
GGGGGCATTAAAACCATTAGCCAAGCCCGTGAATTATTAGCCTTTGGGGCAGATAAGATTTCGATTAACTCGCCCGCCTT
AACCGATCCGAGTTTGATCTCGCGCCTGCAGGACGAGTTCGGCCGCCAGTGTATCGTGATTGGCATCGACTCCTTTTTCG
ATGCCACCAGCAACAGCTATAAGGTAAAGCAATTTACCGGCGATGAAGCGGCGACTAAGGATACTCAGTGGTTCACCCAA
GACTGGGTTGAAGAAGTGCAAAAACGAGGTTGTGGTGAAATCGTGCTCAATGTGATGAACCAAGACGGCGTGCGCGGCGG
CTACGATATCAAGCAGTTAAGCCTTGTTCGCGCCATCTGCGACGTGCCTTTAATTGCCTCTGGCGGTGCTGGGACTATGG
CGCATTTTCGCGATGTGTTTATTGAAGCTAAGGTCGATGCCGCGCTGGCAGCGAGCGTATTCCACAAGGCGATTATCAAC
ATCGGTGAATTAAAAGCCTATTTAGCGGCCGAAGACATTGCGATTAGGCGCTAG

Upstream 100 bases:

>100_bases
CTCTTGAGGATGTTGCCGCCGTGCGTGATAGCGGCGCGGCGGGCATCATTATAGGCAAAGCCTTGCTTATCAATCAGTTT
AACGTGGTGGAGGCAATCCA

Downstream 100 bases:

>100_bases
CAATGGCAATTAGACGCTCGCGGTTAATCGTGAATAGCGAGCTAATGCCACCTCTAGATACCGCGACAGTAAGAGCATGA
GCAAGCAACCTGAATATGTC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MLAKRLVPCLDVKDGKVVKGVQFRNHEIVGDIVPLAARYAEEGADELVFYDITASAHERVVDKSWVSRVAEQIDIPFCVA
GGIKTISQARELLAFGADKISINSPALTDPSLISRLQDEFGRQCIVIGIDSFFDATSNSYKVKQFTGDEAATKDTQWFTQ
DWVEEVQKRGCGEIVLNVMNQDGVRGGYDIKQLSLVRAICDVPLIASGGAGTMAHFRDVFIEAKVDAALAASVFHKAIIN
IGELKAYLAAEDIAIRR

Sequences:

>Translated_257_residues
MLAKRLVPCLDVKDGKVVKGVQFRNHEIVGDIVPLAARYAEEGADELVFYDITASAHERVVDKSWVSRVAEQIDIPFCVA
GGIKTISQARELLAFGADKISINSPALTDPSLISRLQDEFGRQCIVIGIDSFFDATSNSYKVKQFTGDEAATKDTQWFTQ
DWVEEVQKRGCGEIVLNVMNQDGVRGGYDIKQLSLVRAICDVPLIASGGAGTMAHFRDVFIEAKVDAALAASVFHKAIIN
IGELKAYLAAEDIAIRR
>Mature_257_residues
MLAKRLVPCLDVKDGKVVKGVQFRNHEIVGDIVPLAARYAEEGADELVFYDITASAHERVVDKSWVSRVAEQIDIPFCVA
GGIKTISQARELLAFGADKISINSPALTDPSLISRLQDEFGRQCIVIGIDSFFDATSNSYKVKQFTGDEAATKDTQWFTQ
DWVEEVQKRGCGEIVLNVMNQDGVRGGYDIKQLSLVRAICDVPLIASGGAGTMAHFRDVFIEAKVDAALAASVFHKAIIN
IGELKAYLAAEDIAIRR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=256, Percent_Identity=74.21875, Blast_Score=400, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=27.7070063694268, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 28106; Mature: 28106

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRLVPCLDVKDGKVVKGVQFRNHEIVGDIVPLAARYAEEGADELVFYDITASAHERV
CCHHHCCCCCCCCCCCEEECEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHH
VDKSWVSRVAEQIDIPFCVAGGIKTISQARELLAFGADKISINSPALTDPSLISRLQDEF
HHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHH
GRQCIVIGIDSFFDATSNSYKVKQFTGDEAATKDTQWFTQDWVEEVQKRGCGEIVLNVMN
CCEEEEEEEHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHC
QDGVRGGYDIKQLSLVRAICDVPLIASGGAGTMAHFRDVFIEAKVDAALAASVFHKAIIN
CCCCCCCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGELKAYLAAEDIAIRR
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLAKRLVPCLDVKDGKVVKGVQFRNHEIVGDIVPLAARYAEEGADELVFYDITASAHERV
CCHHHCCCCCCCCCCCEEECEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHH
VDKSWVSRVAEQIDIPFCVAGGIKTISQARELLAFGADKISINSPALTDPSLISRLQDEF
HHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHH
GRQCIVIGIDSFFDATSNSYKVKQFTGDEAATKDTQWFTQDWVEEVQKRGCGEIVLNVMN
CCEEEEEEEHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHC
QDGVRGGYDIKQLSLVRAICDVPLIASGGAGTMAHFRDVFIEAKVDAALAASVFHKAIIN
CCCCCCCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGELKAYLAAEDIAIRR
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA