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The map label for this gene is iscS [H]

Identifier: 126174610

GI number: 126174610

Start: 2786452

End: 2787666

Strand: Reverse

Name: iscS [H]

Synonym: Sbal_2397

Alternate gene names: 126174610

Gene position: 2787666-2786452 (Counterclockwise)

Preceding gene: 126174611

Following gene: 126174609

Centisome position: 54.37

GC content: 46.67

Gene sequence:

>1215_bases
ATGAAGCTTCCTATCTATTTAGATTATGCCGCCACCACGCCTGTTGATCCAAGAGTCGCGGAAAAGATGTTCCAATGCAT
GACAATGGACGGCATCTTTGGTAATCCAGCCTCACGTTCACATCGCTACGGCTGGCAAGCTGAAGAAGCGGTGGATATTG
CTCGCAATCAAATTGCCGAGTTAATTAACGCCGATCACCGCGAAATTGTGTTTACCTCTGGCGCGACGGAATCGAACAAC
TTAGCGATTAAGGGTGTTGCTCATTTCTACCACAAGAAAGGCAAGCACATCATCACCAGTAAGACTGAACATAAAGCCGT
TCTTGATACCTGTCGCCAATTAGAGCGCGAAGGTTTCGAAGTGACTTATTTAGAGCCTGCGGCAAACGGCATCATTCCGA
TGGAACGTTTAGAAACGGCAATGCGTGATGACACTATCCTTGTCAGCATCATGCACGTGAACAACGAAATCGGTGTGATC
CACGATATCGATGCAATCGGTGAGCTATGCCGTTCAAAGGGCATTATTTTCCACATGGATGCAGCGCAAAGTGCAGGCAA
AGTGCCTATCGATGTGCAAGCCACTAAAGTGGATTTGATCTCGATTTCTGGTCACAAAATGTATGGCCCTAAAGGCATCG
GCGCGCTATATGTTCGTCGTAAGCCACGCATTCGCTTAGAAGCGCAAATGCACGGTGGTGGCCATGAGCGTGGTATGCGT
AGCGGTACGTTACCAACGCATCAAATCGTAGGTTTAGGTGAAGCTGCTGCAATCGCAAAAGCGGAAATGGCCTCTGATGA
CGCTCGTATCGGTGCATTACGTGACAAATTATGGAATGGCATCAAGCACATTGAAGAAACCTATATCAATGGTGATGCGA
TTGAGCGCGTTAGCGGTAGCCTCAACGTCAGCTTCAACTATGTTGAAGGCGAGTCGTTAATGATGGCACTGAAAGATTTA
GCGGTTTCATCGGGTTCGGCTTGTACCTCAGCCAGCCTAGAGCCTAGCTACGTATTACGTGCGCTTGGCTTAAACGATGA
AATGGCACATAGCTCGATTCGTTTCTCTATCGGCCGTTTCACAACCGAAGAAGAAATTGATCACGCTATCGAAGTAATTA
CTCAATCTATTGATAAATTAAGAGAAATGTCTCCTTTGTGGGAAATGTTTAAAGATGGAATCGACCTGAACCAAGTTCAA
TGGGCACACCATTAA

Upstream 100 bases:

>100_bases
AACAAAGAGTCAGTTTATAACTGAACTTTGTTATACGATAAAAATAATTTTTGTACGTTGTATGTAGCCTCGGCAGAGAC
TGCTCAGTACGGAGTGTGTT

Downstream 100 bases:

>100_bases
TTCTGACCTTTACAATAGATAATTTTGGAGCAGTACCATGGCTTACAGTGAAAAAGTGATAGATCATTATGAGAACCCAC
GTAACGTTGGTTCTTTTGAT

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MKLPIYLDYAATTPVDPRVAEKMFQCMTMDGIFGNPASRSHRYGWQAEEAVDIARNQIAELINADHREIVFTSGATESNN
LAIKGVAHFYHKKGKHIITSKTEHKAVLDTCRQLEREGFEVTYLEPAANGIIPMERLETAMRDDTILVSIMHVNNEIGVI
HDIDAIGELCRSKGIIFHMDAAQSAGKVPIDVQATKVDLISISGHKMYGPKGIGALYVRRKPRIRLEAQMHGGGHERGMR
SGTLPTHQIVGLGEAAAIAKAEMASDDARIGALRDKLWNGIKHIEETYINGDAIERVSGSLNVSFNYVEGESLMMALKDL
AVSSGSACTSASLEPSYVLRALGLNDEMAHSSIRFSIGRFTTEEEIDHAIEVITQSIDKLREMSPLWEMFKDGIDLNQVQ
WAHH

Sequences:

>Translated_404_residues
MKLPIYLDYAATTPVDPRVAEKMFQCMTMDGIFGNPASRSHRYGWQAEEAVDIARNQIAELINADHREIVFTSGATESNN
LAIKGVAHFYHKKGKHIITSKTEHKAVLDTCRQLEREGFEVTYLEPAANGIIPMERLETAMRDDTILVSIMHVNNEIGVI
HDIDAIGELCRSKGIIFHMDAAQSAGKVPIDVQATKVDLISISGHKMYGPKGIGALYVRRKPRIRLEAQMHGGGHERGMR
SGTLPTHQIVGLGEAAAIAKAEMASDDARIGALRDKLWNGIKHIEETYINGDAIERVSGSLNVSFNYVEGESLMMALKDL
AVSSGSACTSASLEPSYVLRALGLNDEMAHSSIRFSIGRFTTEEEIDHAIEVITQSIDKLREMSPLWEMFKDGIDLNQVQ
WAHH
>Mature_404_residues
MKLPIYLDYAATTPVDPRVAEKMFQCMTMDGIFGNPASRSHRYGWQAEEAVDIARNQIAELINADHREIVFTSGATESNN
LAIKGVAHFYHKKGKHIITSKTEHKAVLDTCRQLEREGFEVTYLEPAANGIIPMERLETAMRDDTILVSIMHVNNEIGVI
HDIDAIGELCRSKGIIFHMDAAQSAGKVPIDVQATKVDLISISGHKMYGPKGIGALYVRRKPRIRLEAQMHGGGHERGMR
SGTLPTHQIVGLGEAAAIAKAEMASDDARIGALRDKLWNGIKHIEETYINGDAIERVSGSLNVSFNYVEGESLMMALKDL
AVSSGSACTSASLEPSYVLRALGLNDEMAHSSIRFSIGRFTTEEEIDHAIEVITQSIDKLREMSPLWEMFKDGIDLNQVQ
WAHH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=402, Percent_Identity=58.955223880597, Blast_Score=509, Evalue=1e-144,
Organism=Homo sapiens, GI156713448, Length=421, Percent_Identity=31.1163895486936, Blast_Score=186, Evalue=3e-47,
Organism=Escherichia coli, GI48994898, Length=404, Percent_Identity=78.960396039604, Blast_Score=681, Evalue=0.0,
Organism=Escherichia coli, GI1787970, Length=225, Percent_Identity=33.3333333333333, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1789175, Length=215, Percent_Identity=30.6976744186047, Blast_Score=90, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25143064, Length=403, Percent_Identity=61.0421836228288, Blast_Score=502, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI17533177, Length=327, Percent_Identity=33.0275229357798, Blast_Score=156, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6319831, Length=401, Percent_Identity=57.85536159601, Blast_Score=489, Evalue=1e-139,
Organism=Drosophila melanogaster, GI20129463, Length=402, Percent_Identity=58.955223880597, Blast_Score=500, Evalue=1e-142,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 44676; Mature: 44676

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPIYLDYAATTPVDPRVAEKMFQCMTMDGIFGNPASRSHRYGWQAEEAVDIARNQIAE
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LINADHREIVFTSGATESNNLAIKGVAHFYHKKGKHIITSKTEHKAVLDTCRQLEREGFE
HHCCCCCEEEEECCCCCCCCEEEEHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHCCCE
VTYLEPAANGIIPMERLETAMRDDTILVSIMHVNNEIGVIHDIDAIGELCRSKGIIFHMD
EEEECCCCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCEEECHHHHHHHHHHCCEEEEEC
AAQSAGKVPIDVQATKVDLISISGHKMYGPKGIGALYVRRKPRIRLEAQMHGGGHERGMR
CCCCCCCCCEEEEEEEEEEEEECCCEEECCCCCCEEEEECCCCEEEEEEECCCCHHCCCC
SGTLPTHQIVGLGEAAAIAKAEMASDDARIGALRDKLWNGIKHIEETYINGDAIERVSGS
CCCCCCHHEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCC
LNVSFNYVEGESLMMALKDLAVSSGSACTSASLEPSYVLRALGLNDEMAHSSIRFSIGRF
EEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHEEECCCC
TTEEEIDHAIEVITQSIDKLREMSPLWEMFKDGIDLNQVQWAHH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCC
>Mature Secondary Structure
MKLPIYLDYAATTPVDPRVAEKMFQCMTMDGIFGNPASRSHRYGWQAEEAVDIARNQIAE
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LINADHREIVFTSGATESNNLAIKGVAHFYHKKGKHIITSKTEHKAVLDTCRQLEREGFE
HHCCCCCEEEEECCCCCCCCEEEEHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHCCCE
VTYLEPAANGIIPMERLETAMRDDTILVSIMHVNNEIGVIHDIDAIGELCRSKGIIFHMD
EEEECCCCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCEEECHHHHHHHHHHCCEEEEEC
AAQSAGKVPIDVQATKVDLISISGHKMYGPKGIGALYVRRKPRIRLEAQMHGGGHERGMR
CCCCCCCCCEEEEEEEEEEEEECCCEEECCCCCCEEEEECCCCEEEEEEECCCCHHCCCC
SGTLPTHQIVGLGEAAAIAKAEMASDDARIGALRDKLWNGIKHIEETYINGDAIERVSGS
CCCCCCHHEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCC
LNVSFNYVEGESLMMALKDLAVSSGSACTSASLEPSYVLRALGLNDEMAHSSIRFSIGRF
EEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHEEECCCC
TTEEEIDHAIEVITQSIDKLREMSPLWEMFKDGIDLNQVQWAHH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA