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The map label for this gene is proW [H]

Identifier: 126174513

GI number: 126174513

Start: 2675802

End: 2676653

Strand: Direct

Name: proW [H]

Synonym: Sbal_2298

Alternate gene names: 126174513

Gene position: 2675802-2676653 (Clockwise)

Preceding gene: 126174512

Following gene: 126174514

Centisome position: 52.19

GC content: 48.83

Gene sequence:

>852_bases
ATGTTCAGTTTTGATGAAAGAGTGATTCCTTTAGATGTGTGGATCACCAATATAGTTAACTGGTTGGTTGAAGGCTATCG
ATGGTTTTTCCAACTGATCAAATGGCCCGTTGACTTTATTCTGTCTGGTGTGTCAGACGGTCTACTGGCAACCTCCCCTC
TGCTTGTTATTGCCCTGTTTGGCGCCATTGCTTGGCGAGTGTCAGGTTTAAAACTGGCCATCTTTACGCTGTTATCATTG
GTGCTCATCGGTCTGCTCGGTTTCTGGTCAGAAACCATGATAACCCTCGCCATGGTCTTTTCATCCGTGGTGTTCTGCAC
CCTTGTTGGTGTGCCATTTGGGATATGGGCTGGACGCAGTGATCGTGTTAACAACGTTCTACGACCCATTTTGGACGCGA
TGCAAACGACGCCAGCCTTTGTCTATTTGGTCCCCATTGTGATGCTTTTTTCTATTGGCAATACCGCGGGTGTGCTGGCG
ACCATCATTTTTGCCCTGCCACCCTTGGTGAGATTAACCAGTCTCGGGATCAGGCAAGTACACCCAGAATTAGTCGAGGC
TGCGCTCGCCTTTGGGGCAACAAGCTCGCAGGTTTTACTTAAAGTTCAGATACCCCTTGCTTTACCGACCATCATGGCGG
GCCTAAATCAGACCATCATGATGTCGCTCTCCATGGTGGTCATCGCTGCAATGATCGGCGCGGGTGGCCTCGGGGCACCT
GTCGTTCTGGGCTTAAATACACTTGATATTGGTTTAGCCACGGTGGGCGGTCTAGCGATTGTTTTATTAGCCATCATTCT
CGATAGGATCACCCAATCAATGGCGACCAAAAAAAATAAGGACGTAAAATGA

Upstream 100 bases:

>100_bases
TTTCTAAAAATCTGTTTCTTCGTACCTTAAACCGTGCGGAAAAGGGGACCTATTTTGAGCAGTCAGCATCCTAGAATACT
CACTTCAGGAGTTTTATACC

Downstream 100 bases:

>100_bases
GACTTAAAAAAGTAATACTGGGTGCAGCGTTAGCTGCAATGACAGTGACGGGATCCGCAGCGCTTGCAGATACTCTCAAG
CCAGGAGAAGGCATAACCGT

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MFSFDERVIPLDVWITNIVNWLVEGYRWFFQLIKWPVDFILSGVSDGLLATSPLLVIALFGAIAWRVSGLKLAIFTLLSL
VLIGLLGFWSETMITLAMVFSSVVFCTLVGVPFGIWAGRSDRVNNVLRPILDAMQTTPAFVYLVPIVMLFSIGNTAGVLA
TIIFALPPLVRLTSLGIRQVHPELVEAALAFGATSSQVLLKVQIPLALPTIMAGLNQTIMMSLSMVVIAAMIGAGGLGAP
VVLGLNTLDIGLATVGGLAIVLLAIILDRITQSMATKKNKDVK

Sequences:

>Translated_283_residues
MFSFDERVIPLDVWITNIVNWLVEGYRWFFQLIKWPVDFILSGVSDGLLATSPLLVIALFGAIAWRVSGLKLAIFTLLSL
VLIGLLGFWSETMITLAMVFSSVVFCTLVGVPFGIWAGRSDRVNNVLRPILDAMQTTPAFVYLVPIVMLFSIGNTAGVLA
TIIFALPPLVRLTSLGIRQVHPELVEAALAFGATSSQVLLKVQIPLALPTIMAGLNQTIMMSLSMVVIAAMIGAGGLGAP
VVLGLNTLDIGLATVGGLAIVLLAIILDRITQSMATKKNKDVK
>Mature_283_residues
MFSFDERVIPLDVWITNIVNWLVEGYRWFFQLIKWPVDFILSGVSDGLLATSPLLVIALFGAIAWRVSGLKLAIFTLLSL
VLIGLLGFWSETMITLAMVFSSVVFCTLVGVPFGIWAGRSDRVNNVLRPILDAMQTTPAFVYLVPIVMLFSIGNTAGVLA
TIIFALPPLVRLTSLGIRQVHPELVEAALAFGATSSQVLLKVQIPLALPTIMAGLNQTIMMSLSMVVIAAMIGAGGLGAP
VVLGLNTLDIGLATVGGLAIVLLAIILDRITQSMATKKNKDVK

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine/L-proline [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=277, Percent_Identity=55.2346570397112, Blast_Score=295, Evalue=2e-81,
Organism=Escherichia coli, GI1788451, Length=189, Percent_Identity=32.8042328042328, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788449, Length=218, Percent_Identity=25.6880733944954, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1786564, Length=152, Percent_Identity=31.5789473684211, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30376; Mature: 30376

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSFDERVIPLDVWITNIVNWLVEGYRWFFQLIKWPVDFILSGVSDGLLATSPLLVIALF
CCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
GAIAWRVSGLKLAIFTLLSLVLIGLLGFWSETMITLAMVFSSVVFCTLVGVPFGIWAGRS
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCC
DRVNNVLRPILDAMQTTPAFVYLVPIVMLFSIGNTAGVLATIIFALPPLVRLTSLGIRQV
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
HPELVEAALAFGATSSQVLLKVQIPLALPTIMAGLNQTIMMSLSMVVIAAMIGAGGLGAP
CHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCH
VVLGLNTLDIGLATVGGLAIVLLAIILDRITQSMATKKNKDVK
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MFSFDERVIPLDVWITNIVNWLVEGYRWFFQLIKWPVDFILSGVSDGLLATSPLLVIALF
CCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
GAIAWRVSGLKLAIFTLLSLVLIGLLGFWSETMITLAMVFSSVVFCTLVGVPFGIWAGRS
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCC
DRVNNVLRPILDAMQTTPAFVYLVPIVMLFSIGNTAGVLATIIFALPPLVRLTSLGIRQV
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
HPELVEAALAFGATSSQVLLKVQIPLALPTIMAGLNQTIMMSLSMVVIAAMIGAGGLGAP
CHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCH
VVLGLNTLDIGLATVGGLAIVLLAIILDRITQSMATKKNKDVK
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2649479; 9205837; 9278503 [H]