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The map label for this gene is seqA [H]

Identifier: 126174442

GI number: 126174442

Start: 2575674

End: 2576216

Strand: Reverse

Name: seqA [H]

Synonym: Sbal_2226

Alternate gene names: 126174442

Gene position: 2576216-2575674 (Counterclockwise)

Preceding gene: 126174449

Following gene: 126174441

Centisome position: 50.24

GC content: 48.99

Gene sequence:

>543_bases
ATGAAATATATCGAAGTTGATGAAGAGCTCTATCGTCATATTGCCAGCAAAACAGAGCGTATTGGTGAGAGCGCCTCTGA
CATTCTTCGTCGATTACTCGGCCTGTCCGTCGATGCGGTCGAACCACCTCAGCCTTTGGCGATAAGCCAACCAAGTTTAG
AGGCTGAGCCGATTCAAGCTGTGGCGGTGCAAACTGCACCTGCTGTGAATGACTTCAGTCGCTTAGTCGATGAACATCTG
TTATCGCAACAAAAAGGCGCGGTAGGGCGTTTCCTCTTTTTACTCGAAAGTCTGTATCAATTAGCCCCAGAGCAGTTCTC
GCAAATTCTGCAGATCCAAGGGCGCGATCGTTTGTATTTTGCCCGCTCGAAAGAGCAATTACTGGAAGCGAGTGCCTCGG
CGAATCCTAAAGAAATCGGTACCAGTGGTTTCTGGGTGACAAGCAACAACAATACCGCCAAGAAACGCACCATTTTATCT
GAAGTCTTAGTGCAATTTGGCTGTGATGCAACGGTTGCAGCTGGCATCGCCGAGCGCGTTTAA

Upstream 100 bases:

>100_bases
GACATGGATACACTGGTAACCGATCGCAATGATCTGCCCCGATCGCTGTGGTACACTTTTTTCGGTTAAGCCTGTCGAAT
AACAAAGGATAATTAAGGTT

Downstream 100 bases:

>100_bases
TCTGCGGGTGAGCCATTTAATCAGCTATTGAGCTAAGTTTAACCATTGTTTTTAATTGTAGTTGAGTGCAGGAGAGCCAT
AGGTGGCAATACATCAAAGG

Product: replication initiation regulator SeqA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 180; Mature: 180

Protein sequence:

>180_residues
MKYIEVDEELYRHIASKTERIGESASDILRRLLGLSVDAVEPPQPLAISQPSLEAEPIQAVAVQTAPAVNDFSRLVDEHL
LSQQKGAVGRFLFLLESLYQLAPEQFSQILQIQGRDRLYFARSKEQLLEASASANPKEIGTSGFWVTSNNNTAKKRTILS
EVLVQFGCDATVAAGIAERV

Sequences:

>Translated_180_residues
MKYIEVDEELYRHIASKTERIGESASDILRRLLGLSVDAVEPPQPLAISQPSLEAEPIQAVAVQTAPAVNDFSRLVDEHL
LSQQKGAVGRFLFLLESLYQLAPEQFSQILQIQGRDRLYFARSKEQLLEASASANPKEIGTSGFWVTSNNNTAKKRTILS
EVLVQFGCDATVAAGIAERV
>Mature_180_residues
MKYIEVDEELYRHIASKTERIGESASDILRRLLGLSVDAVEPPQPLAISQPSLEAEPIQAVAVQTAPAVNDFSRLVDEHL
LSQQKGAVGRFLFLLESLYQLAPEQFSQILQIQGRDRLYFARSKEQLLEASASANPKEIGTSGFWVTSNNNTAKKRTILS
EVLVQFGCDATVAAGIAERV

Specific function: Negative regulator of replication initiation. Required for sequestration, a process that blocks secondary initiation events. Could block the allosteric transition of dnaA from an inactive form to an active form. Binds hemimethylated oriC. This process has

COG id: COG3057

COG function: function code L; Negative regulator of replication initiationR

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786903, Length=178, Percent_Identity=38.7640449438202, Blast_Score=117, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005621
- InterPro:   IPR010985 [H]

Pfam domain/function: PF03925 SeqA [H]

EC number: NA

Molecular weight: Translated: 19842; Mature: 19842

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYIEVDEELYRHIASKTERIGESASDILRRLLGLSVDAVEPPQPLAISQPSLEAEPIQA
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHH
VAVQTAPAVNDFSRLVDEHLLSQQKGAVGRFLFLLESLYQLAPEQFSQILQIQGRDRLYF
HHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCEEE
ARSKEQLLEASASANPKEIGTSGFWVTSNNNTAKKRTILSEVLVQFGCDATVAAGIAERV
HHHHHHHHHHHCCCCHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure
MKYIEVDEELYRHIASKTERIGESASDILRRLLGLSVDAVEPPQPLAISQPSLEAEPIQA
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHH
VAVQTAPAVNDFSRLVDEHLLSQQKGAVGRFLFLLESLYQLAPEQFSQILQIQGRDRLYF
HHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCEEE
ARSKEQLLEASASANPKEIGTSGFWVTSNNNTAKKRTILSEVLVQFGCDATVAAGIAERV
HHHHHHHHHHHCCCCHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]