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The map label for this gene is gloB [H]

Identifier: 126174220

GI number: 126174220

Start: 2318328

End: 2319119

Strand: Reverse

Name: gloB [H]

Synonym: Sbal_1996

Alternate gene names: 126174220

Gene position: 2319119-2318328 (Counterclockwise)

Preceding gene: 126174223

Following gene: 126174219

Centisome position: 45.23

GC content: 49.12

Gene sequence:

>792_bases
ATGCTCACTATAACAGCGATCAAAGCCTTTAATGACAATTATATCTGGGTGTTACAACAACAACCGCATACACAGGTTTA
TGTCGTCGATCCCGGCGATGCGAGTGTGGTGATTGACTATTTAGAGGCCAATCAACTCACGCTAGCGGGCATTTTACTGA
CCCACCACCACAATGACCATACGGGTGGCGTCGCTGAACTTCAAGCCTACAGTCAAAACCGACTCACAGTGTATGGCCCA
GATAATGAGAAGATTGAAGGCATCACCCATCCGTTGAACGCAACAGCACAGCCCAGATTTATACTCGACTATATGAGTGG
CGAGCTGCAAGTCTTAGATGTACCGGGGCACACAGCAGGACATATTGCCTACGTTATTGCAGATGCCTTATTCTGCGGTG
ATACCCTCTTTAGCGCTGGCTGCGGACGCTTATTTGAAGGTACGCCAGCGCAAATGCTCAACTCACTACAGCAACTTGCC
CAGCTTCCCGCCGACACTCGGGTGTATTGTGCCCATGAGTACACACTTTCAAACCTCAAATTTGCCCTCGCGGTCAATCC
CAACAATCGCGCGCTGCAAGACTATAACGAACGCGCCGTAGCGTTGCGTCGCCAAGACAAAGCGACTATCCCCTCCACCA
TCGCCTTAGAACGCGCCATCAATCCCTTTCTACGTGCCAGCGACACTGAGATTGTTGACAGCATAAAACAGCATTTTAGC
GATCTAAATCATGCCAATTTAGACGAACTTGGCGGTTTTACCCTGCTCAGACAGTGGAAAGATAATTTTTGA

Upstream 100 bases:

>100_bases
CCATTTTGCCACAGGGCAAAAGCGGCAAAGGTCGCCAAGGGCCGTTCACTCATGGATAATACCTTTATCTTCGAATTGAA
ATACAGACATAGGCGGTTCT

Downstream 100 bases:

>100_bases
TCACAAAAGCAATATTATAGATTGAAATTTAAACAGTTTTTTCCATTTTTGATGATGTAAATCAAAAATGGCCTTACATT
ATGTACGATAATTAAGGGAC

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MLTITAIKAFNDNYIWVLQQQPHTQVYVVDPGDASVVIDYLEANQLTLAGILLTHHHNDHTGGVAELQAYSQNRLTVYGP
DNEKIEGITHPLNATAQPRFILDYMSGELQVLDVPGHTAGHIAYVIADALFCGDTLFSAGCGRLFEGTPAQMLNSLQQLA
QLPADTRVYCAHEYTLSNLKFALAVNPNNRALQDYNERAVALRRQDKATIPSTIALERAINPFLRASDTEIVDSIKQHFS
DLNHANLDELGGFTLLRQWKDNF

Sequences:

>Translated_263_residues
MLTITAIKAFNDNYIWVLQQQPHTQVYVVDPGDASVVIDYLEANQLTLAGILLTHHHNDHTGGVAELQAYSQNRLTVYGP
DNEKIEGITHPLNATAQPRFILDYMSGELQVLDVPGHTAGHIAYVIADALFCGDTLFSAGCGRLFEGTPAQMLNSLQQLA
QLPADTRVYCAHEYTLSNLKFALAVNPNNRALQDYNERAVALRRQDKATIPSTIALERAINPFLRASDTEIVDSIKQHFS
DLNHANLDELGGFTLLRQWKDNF
>Mature_263_residues
MLTITAIKAFNDNYIWVLQQQPHTQVYVVDPGDASVVIDYLEANQLTLAGILLTHHHNDHTGGVAELQAYSQNRLTVYGP
DNEKIEGITHPLNATAQPRFILDYMSGELQVLDVPGHTAGHIAYVIADALFCGDTLFSAGCGRLFEGTPAQMLNSLQQLA
QLPADTRVYCAHEYTLSNLKFALAVNPNNRALQDYNERAVALRRQDKATIPSTIALERAINPFLRASDTEIVDSIKQHFS
DLNHANLDELGGFTLLRQWKDNF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538320, Length=270, Percent_Identity=34.8148148148148, Blast_Score=158, Evalue=5e-39,
Organism=Homo sapiens, GI94538322, Length=270, Percent_Identity=34.8148148148148, Blast_Score=157, Evalue=6e-39,
Organism=Homo sapiens, GI14150041, Length=237, Percent_Identity=35.4430379746835, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI21703352, Length=234, Percent_Identity=37.1794871794872, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI116642887, Length=234, Percent_Identity=37.1794871794872, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI46361987, Length=195, Percent_Identity=36.4102564102564, Blast_Score=119, Evalue=2e-27,
Organism=Escherichia coli, GI1786406, Length=238, Percent_Identity=45.3781512605042, Blast_Score=196, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17536925, Length=275, Percent_Identity=30.5454545454545, Blast_Score=130, Evalue=9e-31,
Organism=Saccharomyces cerevisiae, GI6320478, Length=239, Percent_Identity=30.5439330543933, Blast_Score=102, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6324614, Length=241, Percent_Identity=31.1203319502075, Blast_Score=90, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24667711, Length=270, Percent_Identity=36.2962962962963, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI21356335, Length=270, Percent_Identity=36.2962962962963, Blast_Score=168, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24667703, Length=270, Percent_Identity=36.2962962962963, Blast_Score=168, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 29213; Mature: 29213

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTITAIKAFNDNYIWVLQQQPHTQVYVVDPGDASVVIDYLEANQLTLAGILLTHHHNDH
CEEEEEEEEECCCEEEEEECCCCCEEEEECCCCCEEEEEEECCCCEEEEEEEEEECCCCC
TGGVAELQAYSQNRLTVYGPDNEKIEGITHPLNATAQPRFILDYMSGELQVLDVPGHTAG
CCCHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCHH
HIAYVIADALFCGDTLFSAGCGRLFEGTPAQMLNSLQQLAQLPADTRVYCAHEYTLSNLK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEECCEE
FALAVNPNNRALQDYNERAVALRRQDKATIPSTIALERAINPFLRASDTEIVDSIKQHFS
EEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCHHHCCCCHHHHHHHHHHHH
DLNHANLDELGGFTLLRQWKDNF
HCCCCCHHHHCCHHHHHHHCCCC
>Mature Secondary Structure
MLTITAIKAFNDNYIWVLQQQPHTQVYVVDPGDASVVIDYLEANQLTLAGILLTHHHNDH
CEEEEEEEEECCCEEEEEECCCCCEEEEECCCCCEEEEEEECCCCEEEEEEEEEECCCCC
TGGVAELQAYSQNRLTVYGPDNEKIEGITHPLNATAQPRFILDYMSGELQVLDVPGHTAG
CCCHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCHH
HIAYVIADALFCGDTLFSAGCGRLFEGTPAQMLNSLQQLAQLPADTRVYCAHEYTLSNLK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEECCEE
FALAVNPNNRALQDYNERAVALRRQDKATIPSTIALERAINPFLRASDTEIVDSIKQHFS
EEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCHHHCCCCHHHHHHHHHHHH
DLNHANLDELGGFTLLRQWKDNF
HCCCCCHHHHCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA