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The map label for this gene is mltD [H]

Identifier: 126174219

GI number: 126174219

Start: 2316536

End: 2318095

Strand: Reverse

Name: mltD [H]

Synonym: Sbal_1995

Alternate gene names: 126174219

Gene position: 2318095-2316536 (Counterclockwise)

Preceding gene: 126174220

Following gene: 126174215

Centisome position: 45.21

GC content: 45.96

Gene sequence:

>1560_bases
ATGAGAGTATCACTTTATGTTGTTGTAGGGGGATTTGCCCTACTCACAGGTTGTCAGACATTAGACAACCATGGCGTTAA
TCAGACAGTTAATCAGACAGAAGTTGAAGCCGTCACAACCGAAAATACAGTCCCAGAATATTACCTAGCAACTGAGGCTC
CGTCGGCGCAAATTACCGATGTGTGGGAGCGAATTCGTCAAGGTATGCAGTTACCGGTTCCAGATCAAAAATTGGTGAAC
CAATATCGCGATTGGTACATCAAAAATCCGCAGCATCTGGCGATCATCTCAGAGCGTGCAGCACCTTATATGTACCTGAT
CGTGGAAGAGCTTGAAAAGCGTCATCTTCCAATTGAGATCGCCCTATTACCTATTATTGAAAGCGCCTTTGATCCATCAG
CGTCCTCAGCAAGTGCGGCATCGGGTTTGTGGCAGTTCACCACGCCAATGGCCAGCCATTTCGGTTTAGAAATGAACTGG
TGGTACGACGGTCGCCGTGATGTACCCGCTTCGACGACTGCGGCGCTCGATCTGTTGGAATATCTCTACGATAAAACCAA
TAACAATTGGCTTTATGCTATTGCGGCCTATAACTCGGGTGAATCTCGGGTGCTCAATGCGATTAAACGTAACGAGAAGA
AAGGCTTAAAAACTGACTTTTGGTCATTGGATTTACCCAGTGAAACCGAACGCTACGTACCGCAACTGCTGGCGCTGGCC
GAAGTCATTAAACACGCCGATGAATATGGCATCACTTTAGCGCCAATCAGCAACAATCCTTCAATTGAAGTGGTTGATAT
TGATAGCCAAATTGATTTAGCCATGGCGGCAGGTTTGGCAAACATGACCACACTTGAGCTGCAAAAACTCAACCCTGGTT
ATAACCGCTGGGCCACTTCGCCACTGGGGCCGCACACTTTAGTATTACCGATTGATAAATCGGAAGAATTTAAAAAGGCG
CTCGCCGAAACAGAAACCGAAGATAGGATCAGTTGGCTACGCTACACGATCAAATCAGGTGACAGTATTGGCAAAATTGC
GAAGCAGCACCACACGACGATTAATGCAATTCGCGCCGCCAACGGCATGAAAAACAATACCATAGTCGCGGGCAAGCATT
TGATTATTCCTGTCGATGCGCAAGACAAACAGCTCTATGCCCTGTCGACTCAGCAACAATTGCCGAAGAAATCTCACGCT
GCGACTGAATCGACAAAAATTGCCTATCAGGTTAAGTCTGGTGACACACTTTCTGAAATCGCCCAAGCCCATAAAGTGAC
AGTGAAGCAACTTAGCGTTTGGAACAAATTAGGTAAGAGTGACAAGCTACAAAATGGTCAAAAACTGGTGATCTTAGCGC
CGAAAAATCTTGAGCAAACTGCGCAAATTCGTACTGTGAGTTATAAGGTCAAATCCGGTGATTCACTCGCCCGCATCGCC
AGCAAATTTAACGTGACTGTGGCCGAGCTATTAGAATGGAATAGCCTAGCGCAATCACAATATTTGCAACCAGGCCAAGT
GATTAAATTAGTAGTCGATGAAAGTAAGTTAAACGCATAA

Upstream 100 bases:

>100_bases
TTGCGTGAAAACTTTGCGCGCTGCGTCGTCTTTATCTTCATATAAAACATACTGTTACTATAAAGAAAAGCTATGCACTA
ACAGAGGACTACCGTTTTCA

Downstream 100 bases:

>100_bases
GGCTAAACAGGTTCATAGTTCGAACTGATTGGTGCAATATAAACCCTTAGCAAGATTGAGCCACAGATAACAAAAAGGCA
GCAACCCTATTGGATTGCTG

Product: MltD domain-containing protein

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MRVSLYVVVGGFALLTGCQTLDNHGVNQTVNQTEVEAVTTENTVPEYYLATEAPSAQITDVWERIRQGMQLPVPDQKLVN
QYRDWYIKNPQHLAIISERAAPYMYLIVEELEKRHLPIEIALLPIIESAFDPSASSASAASGLWQFTTPMASHFGLEMNW
WYDGRRDVPASTTAALDLLEYLYDKTNNNWLYAIAAYNSGESRVLNAIKRNEKKGLKTDFWSLDLPSETERYVPQLLALA
EVIKHADEYGITLAPISNNPSIEVVDIDSQIDLAMAAGLANMTTLELQKLNPGYNRWATSPLGPHTLVLPIDKSEEFKKA
LAETETEDRISWLRYTIKSGDSIGKIAKQHHTTINAIRAANGMKNNTIVAGKHLIIPVDAQDKQLYALSTQQQLPKKSHA
ATESTKIAYQVKSGDTLSEIAQAHKVTVKQLSVWNKLGKSDKLQNGQKLVILAPKNLEQTAQIRTVSYKVKSGDSLARIA
SKFNVTVAELLEWNSLAQSQYLQPGQVIKLVVDESKLNA

Sequences:

>Translated_519_residues
MRVSLYVVVGGFALLTGCQTLDNHGVNQTVNQTEVEAVTTENTVPEYYLATEAPSAQITDVWERIRQGMQLPVPDQKLVN
QYRDWYIKNPQHLAIISERAAPYMYLIVEELEKRHLPIEIALLPIIESAFDPSASSASAASGLWQFTTPMASHFGLEMNW
WYDGRRDVPASTTAALDLLEYLYDKTNNNWLYAIAAYNSGESRVLNAIKRNEKKGLKTDFWSLDLPSETERYVPQLLALA
EVIKHADEYGITLAPISNNPSIEVVDIDSQIDLAMAAGLANMTTLELQKLNPGYNRWATSPLGPHTLVLPIDKSEEFKKA
LAETETEDRISWLRYTIKSGDSIGKIAKQHHTTINAIRAANGMKNNTIVAGKHLIIPVDAQDKQLYALSTQQQLPKKSHA
ATESTKIAYQVKSGDTLSEIAQAHKVTVKQLSVWNKLGKSDKLQNGQKLVILAPKNLEQTAQIRTVSYKVKSGDSLARIA
SKFNVTVAELLEWNSLAQSQYLQPGQVIKLVVDESKLNA
>Mature_519_residues
MRVSLYVVVGGFALLTGCQTLDNHGVNQTVNQTEVEAVTTENTVPEYYLATEAPSAQITDVWERIRQGMQLPVPDQKLVN
QYRDWYIKNPQHLAIISERAAPYMYLIVEELEKRHLPIEIALLPIIESAFDPSASSASAASGLWQFTTPMASHFGLEMNW
WYDGRRDVPASTTAALDLLEYLYDKTNNNWLYAIAAYNSGESRVLNAIKRNEKKGLKTDFWSLDLPSETERYVPQLLALA
EVIKHADEYGITLAPISNNPSIEVVDIDSQIDLAMAAGLANMTTLELQKLNPGYNRWATSPLGPHTLVLPIDKSEEFKKA
LAETETEDRISWLRYTIKSGDSIGKIAKQHHTTINAIRAANGMKNNTIVAGKHLIIPVDAQDKQLYALSTQQQLPKKSHA
ATESTKIAYQVKSGDTLSEIAQAHKVTVKQLSVWNKLGKSDKLQNGQKLVILAPKNLEQTAQIRTVSYKVKSGDSLARIA
SKFNVTVAELLEWNSLAQSQYLQPGQVIKLVVDESKLNA

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=457, Percent_Identity=32.8227571115974, Blast_Score=263, Evalue=2e-71,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 57782; Mature: 57782

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVSLYVVVGGFALLTGCQTLDNHGVNQTVNQTEVEAVTTENTVPEYYLATEAPSAQITD
CEEEEEEEECHHHHHHHHHHHHCCCCCCCCCCHHEEEEECCCCCCCEEEECCCCCCHHHH
VWERIRQGMQLPVPDQKLVNQYRDWYIKNPQHLAIISERAAPYMYLIVEELEKRHLPIEI
HHHHHHCCCCCCCCCHHHHHHHHCCEECCCCEEEEEECCCCCEEEHHHHHHHHCCCCEEE
ALLPIIESAFDPSASSASAASGLWQFTTPMASHFGLEMNWWYDGRRDVPASTTAALDLLE
EEEHHHHHHCCCCCCCCHHHCCCEECCCHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHH
YLYDKTNNNWLYAIAAYNSGESRVLNAIKRNEKKGLKTDFWSLDLPSETERYVPQLLALA
HHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHH
EVIKHADEYGITLAPISNNPSIEVVDIDSQIDLAMAAGLANMTTLELQKLNPGYNRWATS
HHHHHHHHCCEEEEECCCCCCEEEEEECCCHHHHHHHCCCCCEEEEEEECCCCCCCCCCC
PLGPHTLVLPIDKSEEFKKALAETETEDRISWLRYTIKSGDSIGKIAKQHHTTINAIRAA
CCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
NGMKNNTIVAGKHLIIPVDAQDKQLYALSTQQQLPKKSHAATESTKIAYQVKSGDTLSEI
CCCCCCEEEECCEEEEEECCCCCEEEEECCHHHCCCHHHCCCCCCEEEEEECCCCCHHHH
AQAHKVTVKQLSVWNKLGKSDKLQNGQKLVILAPKNLEQTAQIRTVSYKVKSGDSLARIA
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHEEEEEEEEECCCCHHHHHH
SKFNVTVAELLEWNSLAQSQYLQPGQVIKLVVDESKLNA
HHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure
MRVSLYVVVGGFALLTGCQTLDNHGVNQTVNQTEVEAVTTENTVPEYYLATEAPSAQITD
CEEEEEEEECHHHHHHHHHHHHCCCCCCCCCCHHEEEEECCCCCCCEEEECCCCCCHHHH
VWERIRQGMQLPVPDQKLVNQYRDWYIKNPQHLAIISERAAPYMYLIVEELEKRHLPIEI
HHHHHHCCCCCCCCCHHHHHHHHCCEECCCCEEEEEECCCCCEEEHHHHHHHHCCCCEEE
ALLPIIESAFDPSASSASAASGLWQFTTPMASHFGLEMNWWYDGRRDVPASTTAALDLLE
EEEHHHHHHCCCCCCCCHHHCCCEECCCHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHH
YLYDKTNNNWLYAIAAYNSGESRVLNAIKRNEKKGLKTDFWSLDLPSETERYVPQLLALA
HHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHH
EVIKHADEYGITLAPISNNPSIEVVDIDSQIDLAMAAGLANMTTLELQKLNPGYNRWATS
HHHHHHHHCCEEEEECCCCCCEEEEEECCCHHHHHHHCCCCCEEEEEEECCCCCCCCCCC
PLGPHTLVLPIDKSEEFKKALAETETEDRISWLRYTIKSGDSIGKIAKQHHTTINAIRAA
CCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
NGMKNNTIVAGKHLIIPVDAQDKQLYALSTQQQLPKKSHAATESTKIAYQVKSGDTLSEI
CCCCCCEEEECCEEEEEECCCCCEEEEECCHHHCCCHHHCCCCCCEEEEEECCCCCHHHH
AQAHKVTVKQLSVWNKLGKSDKLQNGQKLVILAPKNLEQTAQIRTVSYKVKSGDSLARIA
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHEEEEEEEEECCCCHHHHHH
SKFNVTVAELLEWNSLAQSQYLQPGQVIKLVVDESKLNA
HHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]