Click here to switch to the map view.

The map label for this gene is iadA [H]

Identifier: 126174208

GI number: 126174208

Start: 2300383

End: 2301552

Strand: Reverse

Name: iadA [H]

Synonym: Sbal_1984

Alternate gene names: 126174208

Gene position: 2301552-2300383 (Counterclockwise)

Preceding gene: 126174209

Following gene: 126174207

Centisome position: 44.89

GC content: 48.29

Gene sequence:

>1170_bases
ATGTTAACTTTAATCCGTAACGCCGCCGTTTATGCGCCGCAGTTTTTAGGGCTACGCGACGTGTTATTTTGCGAGGCGAA
AGTCCTTAAAATCGCCCCCGTCATTGATCTTAAGCATGCAGACTTAGTCACAATCATCGAAGCCAATGGCCGCCCTTTAA
TCCCAGGTTTAGTGGACCCCTTAGTGCATATCACAGGCGGTGGCGGTGAAGGCGGTTACCACACGCGTACCCCAGAGATG
CAACTAACAGATGCCAGCCTTGCAGGGGTCACTACGCTCGTCGCAGCATTGGGAACCGACGCCATTAGCCGCAGTTTGAA
CGAACTTGTCGTCAAGGCTAAAGCGCTCACATACGAAGGCTTGTCTGTCTTTTGCTACACTGGCTCCTACGAGTACCCTG
TAAAGACCCTTACTGGCGATGTCACCCAAGATCTGGTGTTAATCGACAATGTTATTGGTGTGGGTGAAATTGCAATTGCA
GATCATCGAGGCTCACAACTCACTGTGCCAGAACTCGCCAGAGTCGCAGCACAAAGCCGCGTCGGCGGCATGCTCAGCGG
TAAAGGCGGCATAGTATTTGTGCATGTGGGCGATGGTGCGAGTGGTATTCAAATATTGCATCTCATCGCGCAGCAATCAG
ATATCCCTTTAACGCAGTTCTATCCCACTCACATGAATCGCTCCCAGCAGCTATTAGAGCAAGGGATTGAATTTGCACGA
CAAGGTGGGGTTATCGATTTCACGACGTCGACCAATACTCAGTATCTAAACGAGGGTGAAGTCGCGGTCGTTAATGCGGT
CCATTTTGCAATCTCGCAAGGTGTTAACTTGAGTCAAATCAGCTTAAGTTCCGACGGAAATGCTAGCCTGCCCATATTTA
GTTCTGACGGGGAATGTATTGGAATAGAAATGGGTAAAGTCAGCAGCCTATTTGAAGCACTAGTGGCTTTAATTAACAGT
GGTTTAACCCTCGAACAAGTGCTTCCTTTAGTGACCTCAAATGCCGCTCGCGTGCTCAAGCTAAAAACCAAGGGCGGAAT
AAGCGAAACACACGATGCCGACTTTGTGTTATTAACGGACAATTTTAATATCGATAAAGTATGGAGCAAAGGGCAACTAC
TCGTTGATGAAGGTAACGCGCTGAAATGGGGAACATTTGAATCGCATTAA

Upstream 100 bases:

>100_bases
CATACGCTCAATGGCTTGCGCTTGTTTGGCGCTTTCAACTATTACTGACCTTGATGGCCAGTGCCGTCATTTTATTTGCC
GTTATTACAGGATATAGCTG

Downstream 100 bases:

>100_bases
CTCAATAATGGCGCACGCCGCGCGCGTGCACCACACTCATTAACTATGGGCAATGCGAGTAGATGACTCGCAAACAGAGA
GCATCAAGCACATTTTCTCA

Product: isoaspartyl dipeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MLTLIRNAAVYAPQFLGLRDVLFCEAKVLKIAPVIDLKHADLVTIIEANGRPLIPGLVDPLVHITGGGGEGGYHTRTPEM
QLTDASLAGVTTLVAALGTDAISRSLNELVVKAKALTYEGLSVFCYTGSYEYPVKTLTGDVTQDLVLIDNVIGVGEIAIA
DHRGSQLTVPELARVAAQSRVGGMLSGKGGIVFVHVGDGASGIQILHLIAQQSDIPLTQFYPTHMNRSQQLLEQGIEFAR
QGGVIDFTTSTNTQYLNEGEVAVVNAVHFAISQGVNLSQISLSSDGNASLPIFSSDGECIGIEMGKVSSLFEALVALINS
GLTLEQVLPLVTSNAARVLKLKTKGGISETHDADFVLLTDNFNIDKVWSKGQLLVDEGNALKWGTFESH

Sequences:

>Translated_389_residues
MLTLIRNAAVYAPQFLGLRDVLFCEAKVLKIAPVIDLKHADLVTIIEANGRPLIPGLVDPLVHITGGGGEGGYHTRTPEM
QLTDASLAGVTTLVAALGTDAISRSLNELVVKAKALTYEGLSVFCYTGSYEYPVKTLTGDVTQDLVLIDNVIGVGEIAIA
DHRGSQLTVPELARVAAQSRVGGMLSGKGGIVFVHVGDGASGIQILHLIAQQSDIPLTQFYPTHMNRSQQLLEQGIEFAR
QGGVIDFTTSTNTQYLNEGEVAVVNAVHFAISQGVNLSQISLSSDGNASLPIFSSDGECIGIEMGKVSSLFEALVALINS
GLTLEQVLPLVTSNAARVLKLKTKGGISETHDADFVLLTDNFNIDKVWSKGQLLVDEGNALKWGTFESH
>Mature_389_residues
MLTLIRNAAVYAPQFLGLRDVLFCEAKVLKIAPVIDLKHADLVTIIEANGRPLIPGLVDPLVHITGGGGEGGYHTRTPEM
QLTDASLAGVTTLVAALGTDAISRSLNELVVKAKALTYEGLSVFCYTGSYEYPVKTLTGDVTQDLVLIDNVIGVGEIAIA
DHRGSQLTVPELARVAAQSRVGGMLSGKGGIVFVHVGDGASGIQILHLIAQQSDIPLTQFYPTHMNRSQQLLEQGIEFAR
QGGVIDFTTSTNTQYLNEGEVAVVNAVHFAISQGVNLSQISLSSDGNASLPIFSSDGECIGIEMGKVSSLFEALVALINS
GLTLEQVLPLVTSNAARVLKLKTKGGISETHDADFVLLTDNFNIDKVWSKGQLLVDEGNALKWGTFESH

Specific function: Catalyzes the hydrolytic cleavage of a subset of L- isoaspartyl (L-beta-aspartyl) dipeptides. Used to degrade proteins damaged by L-isoaspartyl residues formation. The best substrate for the enzyme reported thus far is iso-Asp-Leu [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M38 family [H]

Homologues:

Organism=Escherichia coli, GI1790784, Length=388, Percent_Identity=39.4329896907216, Blast_Score=285, Evalue=5e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR011059
- InterPro:   IPR010229 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: 3.4.19.- [C]

Molecular weight: Translated: 41319; Mature: 41319

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTLIRNAAVYAPQFLGLRDVLFCEAKVLKIAPVIDLKHADLVTIIEANGRPLIPGLVDP
CEEEEECCHHHCHHHCCHHHHHHHHHEEEEEEEEEEECCCCEEEEEECCCCEECCHHHCC
LVHITGGGGEGGYHTRTPEMQLTDASLAGVTTLVAALGTDAISRSLNELVVKAKALTYEG
EEEEECCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECC
LSVFCYTGSYEYPVKTLTGDVTQDLVLIDNVIGVGEIAIADHRGSQLTVPELARVAAQSR
CEEEEEECCCCCCHHHHCCCHHHHHHEECCCCCCCEEEEECCCCCEECHHHHHHHHHHHH
VGGMLSGKGGIVFVHVGDGASGIQILHLIAQQSDIPLTQFYPTHMNRSQQLLEQGIEFAR
CCCEEECCCCEEEEEECCCCCHHHHHHHHHHCCCCCHHEECCCCCCHHHHHHHHHHHHHH
QGGVIDFTTSTNTQYLNEGEVAVVNAVHFAISQGVNLSQISLSSDGNASLPIFSSDGECI
CCCEEEEECCCCCEEECCCCEEEEEEEHHHHHCCCCEEEEEECCCCCCCCEEECCCCCEE
GIEMGKVSSLFEALVALINSGLTLEQVLPLVTSNAARVLKLKTKGGISETHDADFVLLTD
EEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCEEEEEC
NFNIDKVWSKGQLLVDEGNALKWGTFESH
CCCHHEECCCCEEEEECCCEEECCCCCCC
>Mature Secondary Structure
MLTLIRNAAVYAPQFLGLRDVLFCEAKVLKIAPVIDLKHADLVTIIEANGRPLIPGLVDP
CEEEEECCHHHCHHHCCHHHHHHHHHEEEEEEEEEEECCCCEEEEEECCCCEECCHHHCC
LVHITGGGGEGGYHTRTPEMQLTDASLAGVTTLVAALGTDAISRSLNELVVKAKALTYEG
EEEEECCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECC
LSVFCYTGSYEYPVKTLTGDVTQDLVLIDNVIGVGEIAIADHRGSQLTVPELARVAAQSR
CEEEEEECCCCCCHHHHCCCHHHHHHEECCCCCCCEEEEECCCCCEECHHHHHHHHHHHH
VGGMLSGKGGIVFVHVGDGASGIQILHLIAQQSDIPLTQFYPTHMNRSQQLLEQGIEFAR
CCCEEECCCCEEEEEECCCCCHHHHHHHHHHCCCCCHHEECCCCCCHHHHHHHHHHHHHH
QGGVIDFTTSTNTQYLNEGEVAVVNAVHFAISQGVNLSQISLSSDGNASLPIFSSDGECI
CCCEEEEECCCCCEEECCCCEEEEEEEHHHHHCCCCEEEEEECCCCCCCCEEECCCCCEE
GIEMGKVSSLFEALVALINSGLTLEQVLPLVTSNAARVLKLKTKGGISETHDADFVLLTD
EEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCEEEEEC
NFNIDKVWSKGQLLVDEGNALKWGTFESH
CCCHHEECCCCEEEEECCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7876157; 7610040; 9278503 [H]