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The map label for this gene is hydC [H]

Identifier: 126174113

GI number: 126174113

Start: 2187601

End: 2188275

Strand: Reverse

Name: hydC [H]

Synonym: Sbal_1887

Alternate gene names: 126174113

Gene position: 2188275-2187601 (Counterclockwise)

Preceding gene: 126174114

Following gene: 126174112

Centisome position: 42.68

GC content: 47.85

Gene sequence:

>675_bases
ATGAACCATTCTGCAACCCGCATTCGGACACTGGTTTTTAGTCCGGCAATACGGATTTTCCACTGGCTCAGGGCACTATC
AATTTTAGTGCTGGTGATCACCGGATTCTATATTGCTTGGCCGTTTCTTGTGGCGCCTGAAAGTACGGATGTGCTCGTAC
AAGGCTGGATCCGCTTCGCGCATCTGATCTGCGGTTTTGTATTGACCGCCATCACCTTAGTCCGCTTCTACCTGTATTTT
TTCAGTCGAAGCGACATTGAGCGGCGCTCATTTCGCGATGTGATGAGTGTAAAAAGCTGGATCACTCAGCTGAAATCTTA
CTTATGGATGGGTCACTTACATAAAGCGGGTGTATATGGACCGCTGCAATTTGTGACTTATGTAGCAATCTCACTGGTCG
CTCTCGTGATATGTATAACGGGTCTGGTGTTGTACGCCAATGTGTATCACCAAGGTTTAGGTGGGATGCTTTGGGGCAGC
GCCGCTTGGATTACGGCACAAATGGGTGGGCTGGCACAGGTTAGGATTTTCCATCACTACTTAACTTGGGCATTTGTTAT
CTTTGTGGTTATCCACGTCTATATGGCGGTTTGGTCAGGGATACGCTTCAAACATAACTCAGTTGACTCGATTGTCTCTG
GCTATGACTACCCAAAGCCAGACTCACACCACTAG

Upstream 100 bases:

>100_bases
TGCATGGCGTGTTCTGTACACGTGATGGATTTTAAAGGTCAGGCATTGAGCGAGTTCCGTGTCAGTCCCAATGGTCAATA
ATCGAGGGAGGGAACGCAAA

Downstream 100 bases:

>100_bases
GAGTCCAATGAAGATATTGCTACTCGGTATTGGCAATGTCCTGTACGCCGATGAAGGCATCGGCGTACATTTTGTCAATT
ATATAGCTGATAATTATCAG

Product: Ni/Fe-hydrogenase, b-type cytochrome subunit

Products: oxidized ferredoxin; H2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MNHSATRIRTLVFSPAIRIFHWLRALSILVLVITGFYIAWPFLVAPESTDVLVQGWIRFAHLICGFVLTAITLVRFYLYF
FSRSDIERRSFRDVMSVKSWITQLKSYLWMGHLHKAGVYGPLQFVTYVAISLVALVICITGLVLYANVYHQGLGGMLWGS
AAWITAQMGGLAQVRIFHHYLTWAFVIFVVIHVYMAVWSGIRFKHNSVDSIVSGYDYPKPDSHH

Sequences:

>Translated_224_residues
MNHSATRIRTLVFSPAIRIFHWLRALSILVLVITGFYIAWPFLVAPESTDVLVQGWIRFAHLICGFVLTAITLVRFYLYF
FSRSDIERRSFRDVMSVKSWITQLKSYLWMGHLHKAGVYGPLQFVTYVAISLVALVICITGLVLYANVYHQGLGGMLWGS
AAWITAQMGGLAQVRIFHHYLTWAFVIFVVIHVYMAVWSGIRFKHNSVDSIVSGYDYPKPDSHH
>Mature_224_residues
MNHSATRIRTLVFSPAIRIFHWLRALSILVLVITGFYIAWPFLVAPESTDVLVQGWIRFAHLICGFVLTAITLVRFYLYF
FSRSDIERRSFRDVMSVKSWITQLKSYLWMGHLHKAGVYGPLQFVTYVAISLVALVICITGLVLYANVYHQGLGGMLWGS
AAWITAQMGGLAQVRIFHHYLTWAFVIFVVIHVYMAVWSGIRFKHNSVDSIVSGYDYPKPDSHH

Specific function: B-type cytochrome that interacts with quinones and anchor the hydrogenase to the membrane [H]

COG id: COG1969

COG function: function code C; Ni,Fe-hydrogenase I cytochrome b subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hupC/hyaC/hydC family [H]

Homologues:

Organism=Escherichia coli, GI1787208, Length=220, Percent_Identity=26.3636363636364, Blast_Score=68, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR000516 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: NA

Molecular weight: Translated: 25658; Mature: 25658

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS00882 NI_HGENASE_CYTB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHSATRIRTLVFSPAIRIFHWLRALSILVLVITGFYIAWPFLVAPESTDVLVQGWIRFA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
HLICGFVLTAITLVRFYLYFFSRSDIERRSFRDVMSVKSWITQLKSYLWMGHLHKAGVYG
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
PLQFVTYVAISLVALVICITGLVLYANVYHQGLGGMLWGSAAWITAQMGGLAQVRIFHHY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHH
LTWAFVIFVVIHVYMAVWSGIRFKHNSVDSIVSGYDYPKPDSHH
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNHSATRIRTLVFSPAIRIFHWLRALSILVLVITGFYIAWPFLVAPESTDVLVQGWIRFA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
HLICGFVLTAITLVRFYLYFFSRSDIERRSFRDVMSVKSWITQLKSYLWMGHLHKAGVYG
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
PLQFVTYVAISLVALVICITGLVLYANVYHQGLGGMLWGSAAWITAQMGGLAQVRIFHHY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHH
LTWAFVIFVVIHVYMAVWSGIRFKHNSVDSIVSGYDYPKPDSHH
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced ferredoxin; 2 H+ [C]

Specific reaction: Reduced ferredoxin + 2 H+ = 2 oxidized ferredoxin + H2 [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1587288; 8319698; 14500908 [H]