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The map label for this gene is hydD [H]

Identifier: 126174112

GI number: 126174112

Start: 2187036

End: 2187593

Strand: Reverse

Name: hydD [H]

Synonym: Sbal_1886

Alternate gene names: 126174112

Gene position: 2187593-2187036 (Counterclockwise)

Preceding gene: 126174113

Following gene: 126174111

Centisome position: 42.66

GC content: 48.57

Gene sequence:

>558_bases
ATGAAGATATTGCTACTCGGTATTGGCAATGTCCTGTACGCCGATGAAGGCATCGGCGTACATTTTGTCAATTATATAGC
TGATAATTATCAGTTTACCCATGAGTCTCACCAGCTGAAGCTGCTCGATGGCGGCACCTTGGCTCAGGGACTCATTCCCA
TTATTTGCCAATACGATTATTTAATCGTGGTCGATACTGTCAATGCCAATGGCGTAGAAGCGGGCGAAGTCTACTTCTTC
GATTTTGATAAGGCACCGAAGGAAATCGACTGGCAAGGCAGCGCCCATGAAGTCGAAATGCTGCAAACCCTCAATATGAT
GGAAATGGTTGGCGACAGACCTAAGACCTTTGTGCTGGGCGTCACCCCAACCGTGCTCGAACCTATGGTGCTGGGCTTGA
CCCCTAAAGTCGCCGCCGCAGTGCCGTTAATGGAATCAAGCTTGTTATCCCACTTAGTCTCCCTTGGGTTTACTGCGACG
CGTATTGCCGATCACAGTATCGAATCGCTGATCCCTGACTCTTATAAACGCGGCGTTACTACTGGTGAAAGTATATAG

Upstream 100 bases:

>100_bases
ATGGCGGTTTGGTCAGGGATACGCTTCAAACATAACTCAGTTGACTCGATTGTCTCTGGCTATGACTACCCAAAGCCAGA
CTCACACCACTAGGAGTCCA

Downstream 100 bases:

>100_bases
AAAATGAAAAACATCAGATTCGATTTTACCTGCTCACGTCAGGTGCCTTTGTACGCGCATTTATGTAATCAATACCTAAA
TTACGATGCGCTGAACATCA

Product: hydrogenase expression/formation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MKILLLGIGNVLYADEGIGVHFVNYIADNYQFTHESHQLKLLDGGTLAQGLIPIICQYDYLIVVDTVNANGVEAGEVYFF
DFDKAPKEIDWQGSAHEVEMLQTLNMMEMVGDRPKTFVLGVTPTVLEPMVLGLTPKVAAAVPLMESSLLSHLVSLGFTAT
RIADHSIESLIPDSYKRGVTTGESI

Sequences:

>Translated_185_residues
MKILLLGIGNVLYADEGIGVHFVNYIADNYQFTHESHQLKLLDGGTLAQGLIPIICQYDYLIVVDTVNANGVEAGEVYFF
DFDKAPKEIDWQGSAHEVEMLQTLNMMEMVGDRPKTFVLGVTPTVLEPMVLGLTPKVAAAVPLMESSLLSHLVSLGFTAT
RIADHSIESLIPDSYKRGVTTGESI
>Mature_185_residues
MKILLLGIGNVLYADEGIGVHFVNYIADNYQFTHESHQLKLLDGGTLAQGLIPIICQYDYLIVVDTVNANGVEAGEVYFF
DFDKAPKEIDWQGSAHEVEMLQTLNMMEMVGDRPKTFVLGVTPTVLEPMVLGLTPKVAAAVPLMESSLLSHLVSLGFTAT
RIADHSIESLIPDSYKRGVTTGESI

Specific function: Protease Involved In The C-Terminal Processing Of Hybc, The Large Subunit Of Hydrogenase 2. Specifically Cleaves Off A 15 Amino Acid Peptide From The C Terminus Of The Precursor Of Hybc. [C]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1789367, Length=150, Percent_Identity=32.6666666666667, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004419
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 20228; Mature: 20228

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILLLGIGNVLYADEGIGVHFVNYIADNYQFTHESHQLKLLDGGTLAQGLIPIICQYDY
CEEEEEECCCEEEECCCCCEEEEEEHHCCEEEECCCCEEEEEECCCHHHHHHHHHCCCCE
LIVVDTVNANGVEAGEVYFFDFDKAPKEIDWQGSAHEVEMLQTLNMMEMVGDRPKTFVLG
EEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEE
VTPTVLEPMVLGLTPKVAAAVPLMESSLLSHLVSLGFTATRIADHSIESLIPDSYKRGVT
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHCCCC
TGESI
CCCCC
>Mature Secondary Structure
MKILLLGIGNVLYADEGIGVHFVNYIADNYQFTHESHQLKLLDGGTLAQGLIPIICQYDY
CEEEEEECCCEEEECCCCCEEEEEEHHCCEEEECCCCEEEEEECCCHHHHHHHHHCCCCE
LIVVDTVNANGVEAGEVYFFDFDKAPKEIDWQGSAHEVEMLQTLNMMEMVGDRPKTFVLG
EEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEE
VTPTVLEPMVLGLTPKVAAAVPLMESSLLSHLVSLGFTATRIADHSIESLIPDSYKRGVT
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHCCCC
TGESI
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1587288; 9639603; 14500908 [H]