Click here to switch to the map view.

The map label for this gene is bioB

Identifier: 126173985

GI number: 126173985

Start: 2048031

End: 2049083

Strand: Direct

Name: bioB

Synonym: Sbal_1758

Alternate gene names: 126173985

Gene position: 2048031-2049083 (Clockwise)

Preceding gene: 126173982

Following gene: 126173986

Centisome position: 39.94

GC content: 49.1

Gene sequence:

>1053_bases
ATGTCGCAGTTGCAAGTTCGTCATGATTGGAAGCGGGAAGAAATCGAAGCCTTATTTGCGCTGCCGATGAATGATTTGTT
ATTTAAAGCCCACAGCATTCACCGTGAAGTGTACGATCCCAATGAAGTGCAAATTAGCCGCTTGTTATCGATTAAAACCG
GTGCTTGCCCTGAGGATTGTAAATACTGCCCGCAGAGCGCCCGTTATGACACAGGTCTAGAGAAAGAGCGTTTGCTGGCA
ATGGAAACTGTGCTGACCGAAGCGCGCAGCGCTAAAGCCGCGGGGGCTTCTCGTTTCTGCATGGGGGCCGCTTGGCGTAA
CCCAAAAGAAAAAGACATGCCGTACCTTAAGCAAATGGTACAAGAAGTCAAAGCCTTAGGCATGGAAACTTGCATGACGC
TCGGCATGCTTTCTGAAGATCAAGCGAACGATTTAGCCAGCGCAGGCTTAGATTATTACAACCACAATTTAGATACTTCG
CCTGAATACTACGGCGATGTGATCACCACACGTACCTATCAAAATCGTCTAGATACACTGACAAATGTGCGCGCCTCAGG
CATGAAAGTCTGCTCTGGCGGGATTGTCGGCATGGGTGAAAAGGCGACGGATCGTGCAGGCTTACTGCAACAGTTGGCCA
ATTTACCGCAGCACCCTGATTCAGTGCCGATCAATATGTTAGTCAAAGTGGCGGGCACGCCGTTTGAGAAACTTGACGAT
CTCGATCCACTCGAGTTTGTGCGTACTATTGCTGTAGCGCGTATTTTAATGCCACTTTCGCGCGTGCGTTTATCGGCTGG
CCGCGAAAATATGAGTGATGAACTGCAGGCCATGTGTTTCTTTGCTGGCGCCAACTCGATTTTCTACGGTTGCAAATTGC
TGACTACGCCAAACCCAGAAGAAAGCGATGATATGGGGCTATTCCGACGTTTAGGTTTACGTCCAGAGCAGGGCGCTGCG
GCAAAATTGGAAGAAGAAAGTGCAGTATTAGCCAAAGCAGCAGCCTATCAAGATAAGTCATCGGCACAATTCTATGACGC
GGGTGCTTTGTAA

Upstream 100 bases:

>100_bases
AAAATAGCCATTAGTAAACTGGATTCACTGGTTGGCGTTGACAGTTTAAGGGCTGAGGTTATCCTAGCCAAGATAAAAGA
CAGAATAAAAAGGGTGTTCA

Downstream 100 bases:

>100_bases
GAACACTGAGTTCATGTTGTTTGACGCACATTGTTGGATAACATGTCAATCCTGTTGGTCGTAGTCTGTTTTGACTGCGG
CCGACAGGCTTATTAAATGT

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MSQLQVRHDWKREEIEALFALPMNDLLFKAHSIHREVYDPNEVQISRLLSIKTGACPEDCKYCPQSARYDTGLEKERLLA
METVLTEARSAKAAGASRFCMGAAWRNPKEKDMPYLKQMVQEVKALGMETCMTLGMLSEDQANDLASAGLDYYNHNLDTS
PEYYGDVITTRTYQNRLDTLTNVRASGMKVCSGGIVGMGEKATDRAGLLQQLANLPQHPDSVPINMLVKVAGTPFEKLDD
LDPLEFVRTIAVARILMPLSRVRLSAGRENMSDELQAMCFFAGANSIFYGCKLLTTPNPEESDDMGLFRRLGLRPEQGAA
AKLEEESAVLAKAAAYQDKSSAQFYDAGAL

Sequences:

>Translated_350_residues
MSQLQVRHDWKREEIEALFALPMNDLLFKAHSIHREVYDPNEVQISRLLSIKTGACPEDCKYCPQSARYDTGLEKERLLA
METVLTEARSAKAAGASRFCMGAAWRNPKEKDMPYLKQMVQEVKALGMETCMTLGMLSEDQANDLASAGLDYYNHNLDTS
PEYYGDVITTRTYQNRLDTLTNVRASGMKVCSGGIVGMGEKATDRAGLLQQLANLPQHPDSVPINMLVKVAGTPFEKLDD
LDPLEFVRTIAVARILMPLSRVRLSAGRENMSDELQAMCFFAGANSIFYGCKLLTTPNPEESDDMGLFRRLGLRPEQGAA
AKLEEESAVLAKAAAYQDKSSAQFYDAGAL
>Mature_349_residues
SQLQVRHDWKREEIEALFALPMNDLLFKAHSIHREVYDPNEVQISRLLSIKTGACPEDCKYCPQSARYDTGLEKERLLAM
ETVLTEARSAKAAGASRFCMGAAWRNPKEKDMPYLKQMVQEVKALGMETCMTLGMLSEDQANDLASAGLDYYNHNLDTSP
EYYGDVITTRTYQNRLDTLTNVRASGMKVCSGGIVGMGEKATDRAGLLQQLANLPQHPDSVPINMLVKVAGTPFEKLDDL
DPLEFVRTIAVARILMPLSRVRLSAGRENMSDELQAMCFFAGANSIFYGCKLLTTPNPEESDDMGLFRRLGLRPEQGAAA
KLEEESAVLAKAAAYQDKSSAQFYDAGAL

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=345, Percent_Identity=66.3768115942029, Blast_Score=481, Evalue=1e-137,
Organism=Saccharomyces cerevisiae, GI6321725, Length=316, Percent_Identity=46.8354430379747, Blast_Score=312, Evalue=6e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_SHEB2 (B8EAJ2)

Other databases:

- EMBL:   CP001252
- RefSeq:   YP_002358444.1
- ProteinModelPortal:   B8EAJ2
- SMR:   B8EAJ2
- GeneID:   7087063
- GenomeReviews:   CP001252_GR
- KEGG:   sbp:Sbal223_2527
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- ProtClustDB:   CLSK2304484
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 38784; Mature: 38653

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQLQVRHDWKREEIEALFALPMNDLLFKAHSIHREVYDPNEVQISRLLSIKTGACPEDC
CCCCCCHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHH
KYCPQSARYDTGLEKERLLAMETVLTEARSAKAAGASRFCMGAAWRNPKEKDMPYLKQMV
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHH
QEVKALGMETCMTLGMLSEDQANDLASAGLDYYNHNLDTSPEYYGDVITTRTYQNRLDTL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHH
TNVRASGMKVCSGGIVGMGEKATDRAGLLQQLANLPQHPDSVPINMLVKVAGTPFEKLDD
HHHHHCCCEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCHHHHCC
LDPLEFVRTIAVARILMPLSRVRLSAGRENMSDELQAMCFFAGANSIFYGCKLLTTPNPE
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEECEEEEECCCCC
ESDDMGLFRRLGLRPEQGAAAKLEEESAVLAKAAAYQDKSSAQFYDAGAL
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SQLQVRHDWKREEIEALFALPMNDLLFKAHSIHREVYDPNEVQISRLLSIKTGACPEDC
CCCCCHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHH
KYCPQSARYDTGLEKERLLAMETVLTEARSAKAAGASRFCMGAAWRNPKEKDMPYLKQMV
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHH
QEVKALGMETCMTLGMLSEDQANDLASAGLDYYNHNLDTSPEYYGDVITTRTYQNRLDTL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHH
TNVRASGMKVCSGGIVGMGEKATDRAGLLQQLANLPQHPDSVPINMLVKVAGTPFEKLDD
HHHHHCCCEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCHHHHCC
LDPLEFVRTIAVARILMPLSRVRLSAGRENMSDELQAMCFFAGANSIFYGCKLLTTPNPE
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEECEEEEECCCCC
ESDDMGLFRRLGLRPEQGAAAKLEEESAVLAKAAAYQDKSSAQFYDAGAL
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA