Click here to switch to the map view.

The map label for this gene is gph [H]

Identifier: 126173941

GI number: 126173941

Start: 1993692

End: 1994411

Strand: Direct

Name: gph [H]

Synonym: Sbal_1712

Alternate gene names: 126173941

Gene position: 1993692-1994411 (Clockwise)

Preceding gene: 126173940

Following gene: 126173943

Centisome position: 38.88

GC content: 47.08

Gene sequence:

>720_bases
ATGAAGATAAAGCAATACGATTTAGTGATTTTCGATTGGGATGGCACCCTGATGGACTCCATCGGTAAAATTATCACCTG
TATTGAAAATATGGCTAAGGCATTGCAGTTACCTATCCCAACCGAAAGCGATATTCGCGATATCATCGGTCTTTCTATGA
CAGAAGCGCTGCGAGTCCTATTTCCTCAGGGGCTCAATCTCAGCGCTTCTTCTGCCTATTCTCAGCATCAGCAGCATCCT
AAAAATGCATTTAGCCAAGGCGAAGATGATCAATATCAGCAGATGCGCGTTGAGTTTAAAGCGCAATATTTGCATTTAGA
CACCACGCCAACGCCACTCTTTGCACAAGCACCAGTGTTAATCGATGAGTTACATACTCAAGGTTATCAATTAGCCGTTG
CAACCGGTAAGGCCCGTGCGGGACTTGATCGCGTTTTTGCGCAAACGGGCTTAGGTCGATACTTTGTTGCATCACGCTGC
GCCGATGAAGTGCACAGTAAACCCCATCCTGAAATGATTTCAAGCTTGCTGAAAGAATTGAATATTGCCCCGAACCGAGC
CCTAATGGTGGGTGATTCTCTGCTGGATTTGACCATGGCGGCCAATGCCGGGATTGATAGTATTGGCGTGACCTATGGCG
CCCACAGTGCCGACAAGTTACTGCAAGCAGGACCTATCGCTTTGATTGACTCTCCTGCGCAATTGCTGCAATACCTCTAA

Upstream 100 bases:

>100_bases
AATGATGAAGTGATGCGGGTCTCTGCGCCTTTAGATGAAAACTTGTCGCAGTTATTGGATAAGTTAAAGCGTGTGTAATC
GACGGGGATAGGTTTCAAGG

Downstream 100 bases:

>100_bases
CACTGCTATTTAATGCTGAAGCGGCAATACATGCCGCTACGCATGAGTACTTGCTGGTTACCTTTATTTACGTCCCATCT
TTCCGTCAATGAAACGAATC

Product: HAD family hydrolase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MKIKQYDLVIFDWDGTLMDSIGKIITCIENMAKALQLPIPTESDIRDIIGLSMTEALRVLFPQGLNLSASSAYSQHQQHP
KNAFSQGEDDQYQQMRVEFKAQYLHLDTTPTPLFAQAPVLIDELHTQGYQLAVATGKARAGLDRVFAQTGLGRYFVASRC
ADEVHSKPHPEMISSLLKELNIAPNRALMVGDSLLDLTMAANAGIDSIGVTYGAHSADKLLQAGPIALIDSPAQLLQYL

Sequences:

>Translated_239_residues
MKIKQYDLVIFDWDGTLMDSIGKIITCIENMAKALQLPIPTESDIRDIIGLSMTEALRVLFPQGLNLSASSAYSQHQQHP
KNAFSQGEDDQYQQMRVEFKAQYLHLDTTPTPLFAQAPVLIDELHTQGYQLAVATGKARAGLDRVFAQTGLGRYFVASRC
ADEVHSKPHPEMISSLLKELNIAPNRALMVGDSLLDLTMAANAGIDSIGVTYGAHSADKLLQAGPIALIDSPAQLLQYL
>Mature_239_residues
MKIKQYDLVIFDWDGTLMDSIGKIITCIENMAKALQLPIPTESDIRDIIGLSMTEALRVLFPQGLNLSASSAYSQHQQHP
KNAFSQGEDDQYQQMRVEFKAQYLHLDTTPTPLFAQAPVLIDELHTQGYQLAVATGKARAGLDRVFAQTGLGRYFVASRC
ADEVHSKPHPEMISSLLKELNIAPNRALMVGDSLLDLTMAANAGIDSIGVTYGAHSADKLLQAGPIALIDSPAQLLQYL

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=234, Percent_Identity=26.9230769230769, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 26182; Mature: 26182

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKQYDLVIFDWDGTLMDSIGKIITCIENMAKALQLPIPTESDIRDIIGLSMTEALRVL
CCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
FPQGLNLSASSAYSQHQQHPKNAFSQGEDDQYQQMRVEFKAQYLHLDTTPTPLFAQAPVL
CCCCCCCCHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHHHHHEEEECCCCCCCHHCCCHH
IDELHTQGYQLAVATGKARAGLDRVFAQTGLGRYFVASRCADEVHSKPHPEMISSLLKEL
HHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHC
NIAPNRALMVGDSLLDLTMAANAGIDSIGVTYGAHSADKLLQAGPIALIDSPAQLLQYL
CCCCCCEEEECCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCEEEECCHHHHHHCC
>Mature Secondary Structure
MKIKQYDLVIFDWDGTLMDSIGKIITCIENMAKALQLPIPTESDIRDIIGLSMTEALRVL
CCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
FPQGLNLSASSAYSQHQQHPKNAFSQGEDDQYQQMRVEFKAQYLHLDTTPTPLFAQAPVL
CCCCCCCCHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHHHHHEEEECCCCCCCHHCCCHH
IDELHTQGYQLAVATGKARAGLDRVFAQTGLGRYFVASRCADEVHSKPHPEMISSLLKEL
HHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHC
NIAPNRALMVGDSLLDLTMAANAGIDSIGVTYGAHSADKLLQAGPIALIDSPAQLLQYL
CCCCCCEEEECCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCEEEECCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA