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The map label for this gene is mdeA [H]

Identifier: 126173856

GI number: 126173856

Start: 1894149

End: 1895342

Strand: Direct

Name: mdeA [H]

Synonym: Sbal_1622

Alternate gene names: 126173856

Gene position: 1894149-1895342 (Clockwise)

Preceding gene: 126173855

Following gene: 126173857

Centisome position: 36.94

GC content: 49.41

Gene sequence:

>1194_bases
ATGCAAGATGAGTCAGGCAAGATGTGGAAAGCGGCAACACAGGTTATTCATGGCGGCCATGAGCGTGAGGCCTTTGGCGC
CTTAGTAACGCCGCTATATCAAAGTGCGACTTTTGTGTTTGAGTCTGCACAGCAAGGCGGTGAGCGATTTGCGGGTAATG
AGCCGGGGTATATTTACACGCGCTTAGGGAATCCGACAACCGCCGAGCTTGAACGTAAAATGGTGATTTTAGAAGGGGCA
GAGGCAGCCGCTGCAACCGCATCTGGTATGGGCGCAGTATCCGCCGCATTACTGGCTAACTTGCAGATGGGCGATCACTT
AGTGGCATCCAATGCCGTATACGGTTGTACTTATGCGCTAATGATGACTCAGTTCGCTCGCTTTGGGATTGAAGTGTCCC
TAGTGGACTTTTCATCGGTAGTAGAAATAGAAGCGGCGATTAAATCCAATACTAAGGTCATTTTCTGTGAAACTCCAGTG
AATCCCCATTTGCAGGTGTTTGATTTGGCGGCAATTGCGACTATTGCCAAACGTCATCAGTTGGTCAGTATTGTCGATAA
CACTTTTATGACCCCATTACTGCAGAGACCGCTGGATTTTGGTATCGATTTGGTGATCCACAGTGCGACTAAATATCTGA
ATGGTCATGGCGATGTGATTGCGGGGATAGTGTGTGGCAGCGAAGAACAGCTCCATAAAGTGAAGTATGAAATCTTAAAA
GATATTGGCGCTGTGATATCGCCACATGATGCTTGGCTGATCTTGCGCGGCCTTAAAACCTTAGATGTACGTTTACAGCG
CCATTGCGATAGTGCCCAGCGAGTGGCCGAGTTTTTGGCCGAACATCCCAGTATTAACCGCGTGTATTATCCCGGACTTG
CCTCTCACCCAGGGCATAAATTTATTGGTCAGCAGATGAAACGTGCCGGCGGGGTGATCGCGTTTGAACTGGCGGCAAAT
TTAGAAGAGGCTATGGCCTTTGTGGGGCATTTAAGCTTGTTCTCTATTGCGGTGAGCCTAGGCGATGCAGAATCCCTCAT
TCAACATCCAGCGTCGATGACGCACTCTCCCTATACGCCAGAGGCGAGAGCGGCGGCTGGCATTGGTGATAATCTGCTGC
GAATTTCCATTGGCTTAGAAGATTGTGACGACATTATCGCCGACCTGAATCAAGCACTCGCGCGTTTGGCATAA

Upstream 100 bases:

>100_bases
TGTTATCCATTGAATAGTCCATAACGCAGGCATTGAGCATCCCAAACGAACAATCAATCAAGAAGGGGTTCAGTTCAATG
CCAATTACAAGGAGCTAGGT

Downstream 100 bases:

>100_bases
CGAGTGTCACTAATTTAAACCACTAACTATTTGAATGATAAAAGGGATGCGATTTAGCATCCCTTTTTCTATGGTTAGGT
CTAGGTTCATTGGGTTAAGA

Product: methionine gamma-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MQDESGKMWKAATQVIHGGHEREAFGALVTPLYQSATFVFESAQQGGERFAGNEPGYIYTRLGNPTTAELERKMVILEGA
EAAAATASGMGAVSAALLANLQMGDHLVASNAVYGCTYALMMTQFARFGIEVSLVDFSSVVEIEAAIKSNTKVIFCETPV
NPHLQVFDLAAIATIAKRHQLVSIVDNTFMTPLLQRPLDFGIDLVIHSATKYLNGHGDVIAGIVCGSEEQLHKVKYEILK
DIGAVISPHDAWLILRGLKTLDVRLQRHCDSAQRVAEFLAEHPSINRVYYPGLASHPGHKFIGQQMKRAGGVIAFELAAN
LEEAMAFVGHLSLFSIAVSLGDAESLIQHPASMTHSPYTPEARAAAGIGDNLLRISIGLEDCDDIIADLNQALARLA

Sequences:

>Translated_397_residues
MQDESGKMWKAATQVIHGGHEREAFGALVTPLYQSATFVFESAQQGGERFAGNEPGYIYTRLGNPTTAELERKMVILEGA
EAAAATASGMGAVSAALLANLQMGDHLVASNAVYGCTYALMMTQFARFGIEVSLVDFSSVVEIEAAIKSNTKVIFCETPV
NPHLQVFDLAAIATIAKRHQLVSIVDNTFMTPLLQRPLDFGIDLVIHSATKYLNGHGDVIAGIVCGSEEQLHKVKYEILK
DIGAVISPHDAWLILRGLKTLDVRLQRHCDSAQRVAEFLAEHPSINRVYYPGLASHPGHKFIGQQMKRAGGVIAFELAAN
LEEAMAFVGHLSLFSIAVSLGDAESLIQHPASMTHSPYTPEARAAAGIGDNLLRISIGLEDCDDIIADLNQALARLA
>Mature_397_residues
MQDESGKMWKAATQVIHGGHEREAFGALVTPLYQSATFVFESAQQGGERFAGNEPGYIYTRLGNPTTAELERKMVILEGA
EAAAATASGMGAVSAALLANLQMGDHLVASNAVYGCTYALMMTQFARFGIEVSLVDFSSVVEIEAAIKSNTKVIFCETPV
NPHLQVFDLAAIATIAKRHQLVSIVDNTFMTPLLQRPLDFGIDLVIHSATKYLNGHGDVIAGIVCGSEEQLHKVKYEILK
DIGAVISPHDAWLILRGLKTLDVRLQRHCDSAQRVAEFLAEHPSINRVYYPGLASHPGHKFIGQQMKRAGGVIAFELAAN
LEEAMAFVGHLSLFSIAVSLGDAESLIQHPASMTHSPYTPEARAAAGIGDNLLRISIGLEDCDDIIADLNQALARLA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=370, Percent_Identity=40.2702702702703, Blast_Score=269, Evalue=3e-72,
Organism=Homo sapiens, GI299473759, Length=370, Percent_Identity=37.5675675675676, Blast_Score=241, Evalue=6e-64,
Organism=Homo sapiens, GI25453487, Length=369, Percent_Identity=35.5013550135501, Blast_Score=211, Evalue=1e-54,
Organism=Escherichia coli, GI1790375, Length=386, Percent_Identity=39.6373056994819, Blast_Score=271, Evalue=6e-74,
Organism=Escherichia coli, GI1789383, Length=400, Percent_Identity=33.5, Blast_Score=199, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI71980708, Length=374, Percent_Identity=39.8395721925134, Blast_Score=279, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17560056, Length=386, Percent_Identity=42.2279792746114, Blast_Score=274, Evalue=5e-74,
Organism=Caenorhabditis elegans, GI17538055, Length=386, Percent_Identity=41.1917098445596, Blast_Score=270, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6319307, Length=394, Percent_Identity=39.3401015228426, Blast_Score=276, Evalue=5e-75,
Organism=Saccharomyces cerevisiae, GI6323333, Length=426, Percent_Identity=31.6901408450704, Blast_Score=213, Evalue=5e-56,
Organism=Saccharomyces cerevisiae, GI6321254, Length=402, Percent_Identity=31.0945273631841, Blast_Score=162, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6321170, Length=309, Percent_Identity=31.7152103559871, Blast_Score=162, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6321904, Length=412, Percent_Identity=28.6407766990291, Blast_Score=133, Evalue=5e-32,
Organism=Drosophila melanogaster, GI19922554, Length=386, Percent_Identity=39.119170984456, Blast_Score=271, Evalue=6e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 42776; Mature: 42776

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDESGKMWKAATQVIHGGHEREAFGALVTPLYQSATFVFESAQQGGERFAGNEPGYIYT
CCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCEEEE
RLGNPTTAELERKMVILEGAEAAAATASGMGAVSAALLANLQMGDHLVASNAVYGCTYAL
ECCCCCHHHHHCEEEEEECCCHHHHHHCCHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHH
MMTQFARFGIEVSLVDFSSVVEIEAAIKSNTKVIFCETPVNPHLQVFDLAAIATIAKRHQ
HHHHHHHHCCEEEEECHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHH
LVSIVDNTFMTPLLQRPLDFGIDLVIHSATKYLNGHGDVIAGIVCGSEEQLHKVKYEILK
HHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHH
DIGAVISPHDAWLILRGLKTLDVRLQRHCDSAQRVAEFLAEHPSINRVYYPGLASHPGHK
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEECCCCCCCCCHH
FIGQQMKRAGGVIAFELAANLEEAMAFVGHLSLFSIAVSLGDAESLIQHPASMTHSPYTP
HHHHHHHHHCCEEEEHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHCCCCCCCC
EARAAAGIGDNLLRISIGLEDCDDIIADLNQALARLA
CHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQDESGKMWKAATQVIHGGHEREAFGALVTPLYQSATFVFESAQQGGERFAGNEPGYIYT
CCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCEEEE
RLGNPTTAELERKMVILEGAEAAAATASGMGAVSAALLANLQMGDHLVASNAVYGCTYAL
ECCCCCHHHHHCEEEEEECCCHHHHHHCCHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHH
MMTQFARFGIEVSLVDFSSVVEIEAAIKSNTKVIFCETPVNPHLQVFDLAAIATIAKRHQ
HHHHHHHHCCEEEEECHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHH
LVSIVDNTFMTPLLQRPLDFGIDLVIHSATKYLNGHGDVIAGIVCGSEEQLHKVKYEILK
HHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHH
DIGAVISPHDAWLILRGLKTLDVRLQRHCDSAQRVAEFLAEHPSINRVYYPGLASHPGHK
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEECCCCCCCCCHH
FIGQQMKRAGGVIAFELAANLEEAMAFVGHLSLFSIAVSLGDAESLIQHPASMTHSPYTP
HHHHHHHHHCCEEEEHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHCCCCCCCC
EARAAAGIGDNLLRISIGLEDCDDIIADLNQALARLA
CHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]