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The map label for this gene is purU [H]

Identifier: 126173683

GI number: 126173683

Start: 1675219

End: 1676085

Strand: Direct

Name: purU [H]

Synonym: Sbal_1445

Alternate gene names: 126173683

Gene position: 1675219-1676085 (Clockwise)

Preceding gene: 126173682

Following gene: 126173684

Centisome position: 32.67

GC content: 47.52

Gene sequence:

>867_bases
ATGACTGAAACTCGCGGTAATAATTCGCCATCCATAGAACGTAAAATCTTACTGACGGATTGCGCCGATGCCCAAGGCCT
CATCGCCAAAATCACCAGTGTTTGTTTTACCCATAAACTGAATATCATTAAAAACAGTGAGTTTGTGGATAATGCCCAAG
GGCGTTTCTTTATGCGAACTGAGTTAGAGGGCATTTTTAACAGCGAACAGTTATTGCAGGATCTGCGCGACGTGCTGCCT
GCTAAGAATCACATGACCTTGGTCAGCGCCGGCAAAAAGCGCATTGTGGTGTTAGTGACGAAAGAAGCGCACTGTTTAGG
CGATCTATTGATGAAAGCCTATTACGGTGGTTTAAGTGTCGAGATTGCCGCTGTGGTGGGCAACCATGATGCGCTGCGCG
AACTTGCCGAAAAATTTAACATTCCTTTTCATCTGGTGAGTCACGAAGGCTTAGACCGAATTCAACACGAGCAAGCACTG
TTGGCTGCGGTATCTCAATACGAACCCGATTATTTAGTCTTGGCTAAGTACATGCGGGTATTAACGCCTGATTTTGTGGC
GCAATATCCTAATCGCATCATCAACATTCACCATTCTTTCTTACCAGCCTTTATCGGCGCAGCACCCTATCGCCAGGCTT
GGGAGCGCGGGGTGAAAATTATCGGTGCGACGGCGCATTTTGTGAATAATTGCCTCGATGAAGGGCCGATCATCAAGCAG
GACGTAATTCCCGTCGATCACAGTTATAGCGCCTTAGAAATGGCGCGTGCAGGGCGCGATGTTGAAAAGAGTGTGCTGAG
CAAAGCGCTGCAGTTAGTGCTTAACGAGCAAGTGGTCGTGTACGGCAATAAAACGATCGTCTTCTAA

Upstream 100 bases:

>100_bases
CATTTTTATTTTCGCATTATAGGATTTTCATGCTGTTCAGCACGATTTGCTATGGCAAACTCGATGTAGGGACAATATAG
GAGATCCAACTTTGCAACAA

Downstream 100 bases:

>100_bases
AGCGTAGGCAGTCGCGCTAAGGTAATGACAAGCGTAGCGATAACATGACATTCAGTTAAGCCTATTTTAACCCGCGTATG
TCATGGGATCAGCTATCATT

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MTETRGNNSPSIERKILLTDCADAQGLIAKITSVCFTHKLNIIKNSEFVDNAQGRFFMRTELEGIFNSEQLLQDLRDVLP
AKNHMTLVSAGKKRIVVLVTKEAHCLGDLLMKAYYGGLSVEIAAVVGNHDALRELAEKFNIPFHLVSHEGLDRIQHEQAL
LAAVSQYEPDYLVLAKYMRVLTPDFVAQYPNRIINIHHSFLPAFIGAAPYRQAWERGVKIIGATAHFVNNCLDEGPIIKQ
DVIPVDHSYSALEMARAGRDVEKSVLSKALQLVLNEQVVVYGNKTIVF

Sequences:

>Translated_288_residues
MTETRGNNSPSIERKILLTDCADAQGLIAKITSVCFTHKLNIIKNSEFVDNAQGRFFMRTELEGIFNSEQLLQDLRDVLP
AKNHMTLVSAGKKRIVVLVTKEAHCLGDLLMKAYYGGLSVEIAAVVGNHDALRELAEKFNIPFHLVSHEGLDRIQHEQAL
LAAVSQYEPDYLVLAKYMRVLTPDFVAQYPNRIINIHHSFLPAFIGAAPYRQAWERGVKIIGATAHFVNNCLDEGPIIKQ
DVIPVDHSYSALEMARAGRDVEKSVLSKALQLVLNEQVVVYGNKTIVF
>Mature_287_residues
TETRGNNSPSIERKILLTDCADAQGLIAKITSVCFTHKLNIIKNSEFVDNAQGRFFMRTELEGIFNSEQLLQDLRDVLPA
KNHMTLVSAGKKRIVVLVTKEAHCLGDLLMKAYYGGLSVEIAAVVGNHDALRELAEKFNIPFHLVSHEGLDRIQHEQALL
AAVSQYEPDYLVLAKYMRVLTPDFVAQYPNRIINIHHSFLPAFIGAAPYRQAWERGVKIIGATAHFVNNCLDEGPIIKQD
VIPVDHSYSALEMARAGRDVEKSVLSKALQLVLNEQVVVYGNKTIVF

Specific function: Produces formate from formyl-tetrahydrofolate. Provides the major source of formate for the purT-dependent synthesis of 5'-phosphoribosyl-N-formylglycinamide (FGAR) during aerobic growth. Has a role in regulating the one-carbon pool [H]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=209, Percent_Identity=30.1435406698565, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI209869995, Length=209, Percent_Identity=30.1435406698565, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI209869993, Length=209, Percent_Identity=30.1435406698565, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI1787483, Length=278, Percent_Identity=68.3453237410072, Blast_Score=405, Evalue=1e-114,
Organism=Escherichia coli, GI1788846, Length=154, Percent_Identity=35.7142857142857, Blast_Score=88, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17567511, Length=157, Percent_Identity=33.7579617834395, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24582400, Length=199, Percent_Identity=27.6381909547739, Blast_Score=78, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32166; Mature: 32034

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETRGNNSPSIERKILLTDCADAQGLIAKITSVCFTHKLNIIKNSEFVDNAQGRFFMRT
CCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHEECCCHHCCCCCCEEEEE
ELEGIFNSEQLLQDLRDVLPAKNHMTLVSAGKKRIVVLVTKEAHCLGDLLMKAYYGGLSV
HHHHHCCHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEECCHHHHHHHHHHHHHCCCEE
EIAAVVGNHDALRELAEKFNIPFHLVSHEGLDRIQHEQALLAAVSQYEPDYLVLAKYMRV
EEEEEECCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LTPDFVAQYPNRIINIHHSFLPAFIGAAPYRQAWERGVKIIGATAHFVNNCLDEGPIIKQ
HCHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEHHHHHHHHHHCCCCCCHH
DVIPVDHSYSALEMARAGRDVEKSVLSKALQLVLNEQVVVYGNKTIVF
CCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEECCCEEEC
>Mature Secondary Structure 
TETRGNNSPSIERKILLTDCADAQGLIAKITSVCFTHKLNIIKNSEFVDNAQGRFFMRT
CCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHEECCCHHCCCCCCEEEEE
ELEGIFNSEQLLQDLRDVLPAKNHMTLVSAGKKRIVVLVTKEAHCLGDLLMKAYYGGLSV
HHHHHCCHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEECCHHHHHHHHHHHHHCCCEE
EIAAVVGNHDALRELAEKFNIPFHLVSHEGLDRIQHEQALLAAVSQYEPDYLVLAKYMRV
EEEEEECCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LTPDFVAQYPNRIINIHHSFLPAFIGAAPYRQAWERGVKIIGATAHFVNNCLDEGPIIKQ
HCHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEHHHHHHHHHHCCCCCCHH
DVIPVDHSYSALEMARAGRDVEKSVLSKALQLVLNEQVVVYGNKTIVF
CCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 8335255 [H]