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The map label for this gene is aceA [H]

Identifier: 126173555

GI number: 126173555

Start: 1500139

End: 1501464

Strand: Direct

Name: aceA [H]

Synonym: Sbal_1317

Alternate gene names: 126173555

Gene position: 1500139-1501464 (Clockwise)

Preceding gene: 126173554

Following gene: 126173556

Centisome position: 29.26

GC content: 49.02

Gene sequence:

>1326_bases
ATGACTAAGGCAACGACTCAGAATTCACGTCAAGAGCAGATTGATGCAATCAAGAGAGATTGGGCAGAAAACCCACGTTG
GAAAAACGTGCGTCGTCCTTACACCGCGGAAGAAGTGGTTGCACTTCGCGGTTCAATCGTGCCTGAGAATACTATTGCCA
AGCGTGGTGCGGCTAAGTTATGGGAACTGGTTAACGGCGGCGCTAAAAAAGGCTATGTGAATTCGCTCGGTGCGTTAACG
GGCGGTCAAGCGGTACAACAGGCGAAAGCGGGTATCGAAGCGATTTACCTTTCTGGTTGGCAAGTGGCTGCAGATGCTAA
CTTAGCCGGCACTATGTACCCAGATCAATCTTTATACCCAGCGAACTCAGTGCCAGCTGTGGTATCCCGTATCAATAACT
CTTTCCGCCGTGCTGACCAAATTCAATGGGGCAATGGCGTTAATCCAGAAGATGAAAAATTTGTTGATTACTTCCTGCCG
ATTATTGCTGATGCAGAAGCGGGTTTTGGTGGCGTATTAAATGCCTTCGAACTGATGAAGTCGATGATCGATGCGGGCGC
TGCCGGCGTTCACTTTGAAGATCAGTTAGCTTCAGTGAAGAAATGTGGTCACATGGGCGGCAAAGTATTAGTGCCAACCC
AAGAAGCGGTACAAAAGCTGGTTGCTGCACGTTTAGCGGCGGATGTGAGCGGTGTGGATACACTGGTTATCGCCCGTACC
GATGCTAACGCGGCCGATTTGTTAACTTCAGATTGCGACCCATACGACCGTGATTTCGTGACTGGCGAGCGTACTAATGA
AGGTTTCTACCGTGTTAAAGCGGGTCTTGACCAAGCGATTTCTCGCGGTCTTGCCTATGCCCCGTATGCGGATTTGATTT
GGTGTGAAACCGCTAAACCGGATCTTGAAGAAGCGCGTGTTTTTGCTGAAGCGATTCATGCTCAGTACCCAGATCAACTG
TTAGCGTATAACTGTTCGCCTTCGTTCAACTGGAAGAAAAATCTGGACGACGCCACTATCGCGCGCTTCCAACAAGCATT
GTCAGACATGGGCTACAAGTACCAGTTCATCACTTTAGCCGGCATCCACAACATGTGGTACAACATGTTCGACCTCGCTT
ACGACTATGCTCGTGGCGAAGGCATGAAGCATTATGTTGAGAAAGTTCAAGAAGTTGAGTTTGCTGCAGCGAAGAAAGGT
TACACCTTCGTCGCGCATCAACAGGAAGTGGGCACAGGTTATTTTGACCAAGTGACTACGGTTATTCAAGGTGGCCATTC
ATCAGTGACCGCACTGACAGGTTCTACCGAAGAAGAGCAGTTTTAA

Upstream 100 bases:

>100_bases
TTAGCTGTGGCGGGGCTACCGCCAACCAAACAATTTAGCAGAGTGAAACTGGGGGGCAGTTTCATGATAGCTACAACATC
AAAGAGATAGGAGCGACATT

Downstream 100 bases:

>100_bases
GCAGTAAGCAGCGCTTAGGATGATCCATTGTGATTGTGGATCACTCCTCAGAACGCCTTATGCCGATGTATCTGGTGAGG
CGTGGTTAAAAACTCGAAAT

Product: isocitrate lyase

Products: NA

Alternate protein names: ICL; Isocitrase; Isocitratase [H]

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MTKATTQNSRQEQIDAIKRDWAENPRWKNVRRPYTAEEVVALRGSIVPENTIAKRGAAKLWELVNGGAKKGYVNSLGALT
GGQAVQQAKAGIEAIYLSGWQVAADANLAGTMYPDQSLYPANSVPAVVSRINNSFRRADQIQWGNGVNPEDEKFVDYFLP
IIADAEAGFGGVLNAFELMKSMIDAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLVAARLAADVSGVDTLVIART
DANAADLLTSDCDPYDRDFVTGERTNEGFYRVKAGLDQAISRGLAYAPYADLIWCETAKPDLEEARVFAEAIHAQYPDQL
LAYNCSPSFNWKKNLDDATIARFQQALSDMGYKYQFITLAGIHNMWYNMFDLAYDYARGEGMKHYVEKVQEVEFAAAKKG
YTFVAHQQEVGTGYFDQVTTVIQGGHSSVTALTGSTEEEQF

Sequences:

>Translated_441_residues
MTKATTQNSRQEQIDAIKRDWAENPRWKNVRRPYTAEEVVALRGSIVPENTIAKRGAAKLWELVNGGAKKGYVNSLGALT
GGQAVQQAKAGIEAIYLSGWQVAADANLAGTMYPDQSLYPANSVPAVVSRINNSFRRADQIQWGNGVNPEDEKFVDYFLP
IIADAEAGFGGVLNAFELMKSMIDAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLVAARLAADVSGVDTLVIART
DANAADLLTSDCDPYDRDFVTGERTNEGFYRVKAGLDQAISRGLAYAPYADLIWCETAKPDLEEARVFAEAIHAQYPDQL
LAYNCSPSFNWKKNLDDATIARFQQALSDMGYKYQFITLAGIHNMWYNMFDLAYDYARGEGMKHYVEKVQEVEFAAAKKG
YTFVAHQQEVGTGYFDQVTTVIQGGHSSVTALTGSTEEEQF
>Mature_440_residues
TKATTQNSRQEQIDAIKRDWAENPRWKNVRRPYTAEEVVALRGSIVPENTIAKRGAAKLWELVNGGAKKGYVNSLGALTG
GQAVQQAKAGIEAIYLSGWQVAADANLAGTMYPDQSLYPANSVPAVVSRINNSFRRADQIQWGNGVNPEDEKFVDYFLPI
IADAEAGFGGVLNAFELMKSMIDAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLVAARLAADVSGVDTLVIARTD
ANAADLLTSDCDPYDRDFVTGERTNEGFYRVKAGLDQAISRGLAYAPYADLIWCETAKPDLEEARVFAEAIHAQYPDQLL
AYNCSPSFNWKKNLDDATIARFQQALSDMGYKYQFITLAGIHNMWYNMFDLAYDYARGEGMKHYVEKVQEVEFAAAKKGY
TFVAHQQEVGTGYFDQVTTVIQGGHSSVTALTGSTEEEQF

Specific function: Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase- positive serine pathway [H]

COG id: COG2224

COG function: function code C; Isocitrate lyase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1790445, Length=434, Percent_Identity=78.3410138248848, Blast_Score=737, Evalue=0.0,
Organism=Escherichia coli, GI1786525, Length=266, Percent_Identity=28.9473684210526, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71982926, Length=423, Percent_Identity=58.8652482269503, Blast_Score=489, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI17561814, Length=423, Percent_Identity=58.8652482269503, Blast_Score=489, Evalue=1e-138,
Organism=Saccharomyces cerevisiae, GI6320908, Length=302, Percent_Identity=34.7682119205298, Blast_Score=176, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6325263, Length=254, Percent_Identity=37.7952755905512, Blast_Score=161, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006254
- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.1 [H]

Molecular weight: Translated: 48397; Mature: 48265

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00074 GLFV_DEHYDROGENASE ; PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKATTQNSRQEQIDAIKRDWAENPRWKNVRRPYTAEEVVALRGSIVPENTIAKRGAAKL
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHH
WELVNGGAKKGYVNSLGALTGGQAVQQAKAGIEAIYLSGWQVAADANLAGTMYPDQSLYP
HHHHCCCCCCCHHHHHHHCCCHHHHHHHHCCCEEEEECCEEEEECCCCCCCCCCCCCCCC
ANSVPAVVSRINNSFRRADQIQWGNGVNPEDEKFVDYFLPIIADAEAGFGGVLNAFELMK
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
SMIDAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLVAARLAADVSGVDTLVIART
HHHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCCCEEEEEEC
DANAADLLTSDCDPYDRDFVTGERTNEGFYRVKAGLDQAISRGLAYAPYADLIWCETAKP
CCCHHHHHHCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCCCCEEEECCCCC
DLEEARVFAEAIHAQYPDQLLAYNCSPSFNWKKNLDDATIARFQQALSDMGYKYQFITLA
CHHHHHHHHHHHHHHCCCHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEH
GIHNMWYNMFDLAYDYARGEGMKHYVEKVQEVEFAAAKKGYTFVAHQQEVGTGYFDQVTT
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCHHHHHHH
VIQGGHSSVTALTGSTEEEQF
HHHCCCCCEEEEECCCCCCCC
>Mature Secondary Structure 
TKATTQNSRQEQIDAIKRDWAENPRWKNVRRPYTAEEVVALRGSIVPENTIAKRGAAKL
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHH
WELVNGGAKKGYVNSLGALTGGQAVQQAKAGIEAIYLSGWQVAADANLAGTMYPDQSLYP
HHHHCCCCCCCHHHHHHHCCCHHHHHHHHCCCEEEEECCEEEEECCCCCCCCCCCCCCCC
ANSVPAVVSRINNSFRRADQIQWGNGVNPEDEKFVDYFLPIIADAEAGFGGVLNAFELMK
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
SMIDAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLVAARLAADVSGVDTLVIART
HHHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCCCEEEEEEC
DANAADLLTSDCDPYDRDFVTGERTNEGFYRVKAGLDQAISRGLAYAPYADLIWCETAKP
CCCHHHHHHCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCCCCEEEECCCCC
DLEEARVFAEAIHAQYPDQLLAYNCSPSFNWKKNLDDATIARFQQALSDMGYKYQFITLA
CHHHHHHHHHHHHHHCCCHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEH
GIHNMWYNMFDLAYDYARGEGMKHYVEKVQEVEFAAAKKGYTFVAHQQEVGTGYFDQVTT
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCHHHHHHH
VIQGGHSSVTALTGSTEEEQF
HHHCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]