Click here to switch to the map view.

The map label for this gene is lepA [H]

Identifier: 126173437

GI number: 126173437

Start: 1380541

End: 1382331

Strand: Direct

Name: lepA [H]

Synonym: Sbal_1199

Alternate gene names: 126173437

Gene position: 1380541-1382331 (Clockwise)

Preceding gene: 126173436

Following gene: 126173438

Centisome position: 26.92

GC content: 45.39

Gene sequence:

>1791_bases
ATGAAACAAATTAGAAACTTCTCAATTATTGCCCATATCGACCATGGTAAATCGACGCTATCAGATCGTCTTATTCAGGT
TTGTGGCGGTTTAACCGACCGTGAAATGGATGCTCAAGTTTTAGATTCTATGGATCTAGAACGTGAGCGTGGTATTACGA
TTAAGGCACAAAGTGTCACTTTGGATTACAAAGCCAAAGATGGCCTAGTCTATCAACTCAACTTTATTGATACCCCAGGT
CACGTTGACTTCTCCTACGAGGTCTCGCGCTCATTAGCAGCCTGTGAAGGCGCTTTGCTGGTGGTTGATGCGGGTCAGGG
CGTTGAAGCTCAGACACTGGCAAACTGTTACACCGCGCTGGATATGAATCTAGATGTGGTACCTATTTTAAACAAGATTG
ACTTACCACAAGCCGATCCAGAACGTGTTGCTGCCGAGATTGAAGATATCGTCGGTATCGATGCCATGGATGCTGTGCGT
TGTTCGGCTAAAACCGGTGTCGGTGTCGACGAGGTTTTAGAAGTCATTGTTGCCAAAATTCCACCGCCAGAAGGCGATCC
TAACGCACCATTGCAAGCCTTGATCATCGATTCTTGGTTTGATAACTACCTAGGCGTAGTGTCTTTAGTGCGAATTAAAC
ACGGTAGCCTGAAAAAAGGTGACAAGTTTAAGGTGATGTCTACAGGACAAAACCACACTGCGGATCGCGTCGGTATTTTC
ACCCCAAAACAAACTGATAAAACCGAACTTAAAACCGGTGAAGTAGGCTTTGTGATTGCGGGTCTGAAAGAAATTCACGG
CGCGCCTGTGGGCGATACATTAACTTTAGCCAAAAACGGTGCTGAAAAGCCGTTACCTGGTTTTAAGAAAGTAAAGCCAC
AGGTATACGCTGGCGTATTCCCTATTTCAACCGACGAATATGAAAACTTCCGTGATGCGCTGAACAAGCTCAGCCTAAAC
GATGCGTCATTATTCTTCGAACCAGAAAGTTCATCGGCTCTCGGTTTTGGTTTCCGCATTGGCTATTTAGGCCTGCTGCA
CATGGAAATCGTACAAGAGCGTTTAGAACGTGAATACAACCTAGAGTTGATCACCACAGCGCCAACCGTAGTGTACGAAG
TCGTGATGACCAGCGGCGAAACGATTTACGTTGATAACCCGTCTGATCTACCAGCGATTAACAACATCGAAGAAATGCGT
GAGCCGATTGTTGAGGCCAACATTTTAGTGCCAAAAGAATACTTAGGTAACGTGATTACCTTGTGTATTGAAAAACGTGG
TACGCAAGTGAACATGGTTTACCACGGTAACCAAGTGGCTGTGACGTATCATCTGCCAATGGCGGAAGTGGTCATGGACT
TCTTCGACCGTTTGAAATCCACTAGCCGTGGCTATGCATCACTCGAATATAACTTTATTCGTTTCGATCCTGCAGACATG
GTGCGTTTAGATATTCTCATCAACGGCGACAGAGTTGATGCCTTGGCTATGGTTATCCATAGATCCAACATTCGTCACCG
TGGTTTAGCCTTAGTCGATAAGATGAAAGAGCTGATCCCGCGTCAGATGTTTGATATCGCGATTCAAGCGGCTGTGGGCA
GTCAAATTATTGCTCGTTCAACCGTGAAAGCCTTGCGTAAAGACGTAACGGCTAAATGTTATGGCGGCGACGTTTCTCGT
AAGAAAAAACTCTTAAATAAACAAAAAGAGGGTAAGAAACGGATGAAGCAAGTGGGTAACGTTGAGGTGCCACAGGAGGC
ATTCTTAGCGGTACTTAAGCTAAACGAGTAA

Upstream 100 bases:

>100_bases
TCCTAAGTAGGAGTGAATGTGATTGGTATTGAAACCGCAATCAGGTACAATTTCGCTCCTTTGAACCGTATCCATTTTCA
GTATTAGTCATTGCAGCATA

Downstream 100 bases:

>100_bases
TCGTTAGCATAAGAAATGACCATTTAGTTGACTATTGCGCCGCTGTTTGCGGCGCTTTAGTTAGGTTATCGCATTGGTTT
ATCACTTTGGTTTACCAAGG

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
MKQIRNFSIIAHIDHGKSTLSDRLIQVCGGLTDREMDAQVLDSMDLERERGITIKAQSVTLDYKAKDGLVYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTALDMNLDVVPILNKIDLPQADPERVAAEIEDIVGIDAMDAVR
CSAKTGVGVDEVLEVIVAKIPPPEGDPNAPLQALIIDSWFDNYLGVVSLVRIKHGSLKKGDKFKVMSTGQNHTADRVGIF
TPKQTDKTELKTGEVGFVIAGLKEIHGAPVGDTLTLAKNGAEKPLPGFKKVKPQVYAGVFPISTDEYENFRDALNKLSLN
DASLFFEPESSSALGFGFRIGYLGLLHMEIVQERLEREYNLELITTAPTVVYEVVMTSGETIYVDNPSDLPAINNIEEMR
EPIVEANILVPKEYLGNVITLCIEKRGTQVNMVYHGNQVAVTYHLPMAEVVMDFFDRLKSTSRGYASLEYNFIRFDPADM
VRLDILINGDRVDALAMVIHRSNIRHRGLALVDKMKELIPRQMFDIAIQAAVGSQIIARSTVKALRKDVTAKCYGGDVSR
KKKLLNKQKEGKKRMKQVGNVEVPQEAFLAVLKLNE

Sequences:

>Translated_596_residues
MKQIRNFSIIAHIDHGKSTLSDRLIQVCGGLTDREMDAQVLDSMDLERERGITIKAQSVTLDYKAKDGLVYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTALDMNLDVVPILNKIDLPQADPERVAAEIEDIVGIDAMDAVR
CSAKTGVGVDEVLEVIVAKIPPPEGDPNAPLQALIIDSWFDNYLGVVSLVRIKHGSLKKGDKFKVMSTGQNHTADRVGIF
TPKQTDKTELKTGEVGFVIAGLKEIHGAPVGDTLTLAKNGAEKPLPGFKKVKPQVYAGVFPISTDEYENFRDALNKLSLN
DASLFFEPESSSALGFGFRIGYLGLLHMEIVQERLEREYNLELITTAPTVVYEVVMTSGETIYVDNPSDLPAINNIEEMR
EPIVEANILVPKEYLGNVITLCIEKRGTQVNMVYHGNQVAVTYHLPMAEVVMDFFDRLKSTSRGYASLEYNFIRFDPADM
VRLDILINGDRVDALAMVIHRSNIRHRGLALVDKMKELIPRQMFDIAIQAAVGSQIIARSTVKALRKDVTAKCYGGDVSR
KKKLLNKQKEGKKRMKQVGNVEVPQEAFLAVLKLNE
>Mature_596_residues
MKQIRNFSIIAHIDHGKSTLSDRLIQVCGGLTDREMDAQVLDSMDLERERGITIKAQSVTLDYKAKDGLVYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTALDMNLDVVPILNKIDLPQADPERVAAEIEDIVGIDAMDAVR
CSAKTGVGVDEVLEVIVAKIPPPEGDPNAPLQALIIDSWFDNYLGVVSLVRIKHGSLKKGDKFKVMSTGQNHTADRVGIF
TPKQTDKTELKTGEVGFVIAGLKEIHGAPVGDTLTLAKNGAEKPLPGFKKVKPQVYAGVFPISTDEYENFRDALNKLSLN
DASLFFEPESSSALGFGFRIGYLGLLHMEIVQERLEREYNLELITTAPTVVYEVVMTSGETIYVDNPSDLPAINNIEEMR
EPIVEANILVPKEYLGNVITLCIEKRGTQVNMVYHGNQVAVTYHLPMAEVVMDFFDRLKSTSRGYASLEYNFIRFDPADM
VRLDILINGDRVDALAMVIHRSNIRHRGLALVDKMKELIPRQMFDIAIQAAVGSQIIARSTVKALRKDVTAKCYGGDVSR
KKKLLNKQKEGKKRMKQVGNVEVPQEAFLAVLKLNE

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=602, Percent_Identity=48.3388704318937, Blast_Score=608, Evalue=1e-174,
Organism=Homo sapiens, GI25306283, Length=137, Percent_Identity=45.985401459854, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI25306287, Length=137, Percent_Identity=45.985401459854, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI94966754, Length=132, Percent_Identity=44.6969696969697, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI19923640, Length=137, Percent_Identity=45.985401459854, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI4503483, Length=143, Percent_Identity=41.2587412587413, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI18390331, Length=152, Percent_Identity=36.8421052631579, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI53729339, Length=226, Percent_Identity=32.7433628318584, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI53729337, Length=226, Percent_Identity=32.7433628318584, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI310132016, Length=110, Percent_Identity=41.8181818181818, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI310110807, Length=110, Percent_Identity=41.8181818181818, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI310123363, Length=110, Percent_Identity=41.8181818181818, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI217272894, Length=133, Percent_Identity=36.0902255639098, Blast_Score=80, Evalue=6e-15,
Organism=Homo sapiens, GI217272892, Length=133, Percent_Identity=36.0902255639098, Blast_Score=80, Evalue=6e-15,
Organism=Homo sapiens, GI34147630, Length=313, Percent_Identity=27.1565495207668, Blast_Score=67, Evalue=6e-11,
Organism=Escherichia coli, GI1788922, Length=596, Percent_Identity=80.5369127516778, Blast_Score=977, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=508, Percent_Identity=28.1496062992126, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI1789738, Length=159, Percent_Identity=33.9622641509434, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1789559, Length=229, Percent_Identity=31.4410480349345, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790835, Length=156, Percent_Identity=33.3333333333333, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1790412, Length=330, Percent_Identity=24.8484848484848, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789737, Length=330, Percent_Identity=24.8484848484848, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17557151, Length=612, Percent_Identity=40.8496732026144, Blast_Score=488, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI17556745, Length=466, Percent_Identity=24.8927038626609, Blast_Score=112, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17533571, Length=146, Percent_Identity=37.6712328767123, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17506493, Length=157, Percent_Identity=35.6687898089172, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=34.3283582089552, Blast_Score=83, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=34.3283582089552, Blast_Score=83, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17552882, Length=145, Percent_Identity=35.8620689655172, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71994658, Length=226, Percent_Identity=28.3185840707965, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=602, Percent_Identity=46.1794019933555, Blast_Score=547, Evalue=1e-156,
Organism=Saccharomyces cerevisiae, GI6323098, Length=159, Percent_Identity=38.3647798742138, Blast_Score=107, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6324707, Length=143, Percent_Identity=42.6573426573427, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6320593, Length=143, Percent_Identity=42.6573426573427, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322359, Length=115, Percent_Identity=37.3913043478261, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324166, Length=144, Percent_Identity=38.8888888888889, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324761, Length=336, Percent_Identity=25.297619047619, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6325337, Length=161, Percent_Identity=29.1925465838509, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319594, Length=161, Percent_Identity=29.1925465838509, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI78706572, Length=602, Percent_Identity=44.6843853820598, Blast_Score=540, Evalue=1e-154,
Organism=Drosophila melanogaster, GI24582462, Length=161, Percent_Identity=36.0248447204969, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI28574573, Length=138, Percent_Identity=42.7536231884058, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI221458488, Length=150, Percent_Identity=38.6666666666667, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24585709, Length=170, Percent_Identity=34.7058823529412, Blast_Score=94, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24585711, Length=170, Percent_Identity=34.7058823529412, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24585713, Length=170, Percent_Identity=34.7058823529412, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21357743, Length=133, Percent_Identity=36.0902255639098, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI28572034, Length=243, Percent_Identity=29.2181069958848, Blast_Score=74, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 65880; Mature: 65880

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQIRNFSIIAHIDHGKSTLSDRLIQVCGGLTDREMDAQVLDSMDLERERGITIKAQSVT
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHCCCEEEEEEEE
LDYKAKDGLVYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAL
EEEECCCCEEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
DMNLDVVPILNKIDLPQADPERVAAEIEDIVGIDAMDAVRCSAKTGVGVDEVLEVIVAKI
CCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHHHHC
PPPEGDPNAPLQALIIDSWFDNYLGVVSLVRIKHGSLKKGDKFKVMSTGQNHTADRVGIF
CCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCEEEEE
TPKQTDKTELKTGEVGFVIAGLKEIHGAPVGDTLTLAKNGAEKPLPGFKKVKPQVYAGVF
CCCCCCCCCCCCCCCEEEEECHHHHCCCCCCCEEEEECCCCCCCCCCHHHCCCCCEEEEE
PISTDEYENFRDALNKLSLNDASLFFEPESSSALGFGFRIGYLGLLHMEIVQERLEREYN
CCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCC
LELITTAPTVVYEVVMTSGETIYVDNPSDLPAINNIEEMREPIVEANILVPKEYLGNVIT
EEEEECCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCEEECCHHHHHHHHH
LCIEKRGTQVNMVYHGNQVAVTYHLPMAEVVMDFFDRLKSTSRGYASLEYNFIRFDPADM
HHHHCCCCEEEEEEECCEEEEEEECCHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCE
VRLDILINGDRVDALAMVIHRSNIRHRGLALVDKMKELIPRQMFDIAIQAAVGSQIIARS
EEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVKALRKDVTAKCYGGDVSRKKKLLNKQKEGKKRMKQVGNVEVPQEAFLAVLKLNE
HHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEEEEEECC
>Mature Secondary Structure
MKQIRNFSIIAHIDHGKSTLSDRLIQVCGGLTDREMDAQVLDSMDLERERGITIKAQSVT
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHCCCEEEEEEEE
LDYKAKDGLVYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAL
EEEECCCCEEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
DMNLDVVPILNKIDLPQADPERVAAEIEDIVGIDAMDAVRCSAKTGVGVDEVLEVIVAKI
CCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHHHHC
PPPEGDPNAPLQALIIDSWFDNYLGVVSLVRIKHGSLKKGDKFKVMSTGQNHTADRVGIF
CCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCEEEEE
TPKQTDKTELKTGEVGFVIAGLKEIHGAPVGDTLTLAKNGAEKPLPGFKKVKPQVYAGVF
CCCCCCCCCCCCCCCEEEEECHHHHCCCCCCCEEEEECCCCCCCCCCHHHCCCCCEEEEE
PISTDEYENFRDALNKLSLNDASLFFEPESSSALGFGFRIGYLGLLHMEIVQERLEREYN
CCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCC
LELITTAPTVVYEVVMTSGETIYVDNPSDLPAINNIEEMREPIVEANILVPKEYLGNVIT
EEEEECCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCEEECCHHHHHHHHH
LCIEKRGTQVNMVYHGNQVAVTYHLPMAEVVMDFFDRLKSTSRGYASLEYNFIRFDPADM
HHHHCCCCEEEEEEECCEEEEEEECCHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCE
VRLDILINGDRVDALAMVIHRSNIRHRGLALVDKMKELIPRQMFDIAIQAAVGSQIIARS
EEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVKALRKDVTAKCYGGDVSRKKKLLNKQKEGKKRMKQVGNVEVPQEAFLAVLKLNE
HHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA