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The map label for this gene is mdeA [H]

Identifier: 126173161

GI number: 126173161

Start: 1050432

End: 1051730

Strand: Direct

Name: mdeA [H]

Synonym: Sbal_0916

Alternate gene names: 126173161

Gene position: 1050432-1051730 (Clockwise)

Preceding gene: 126173160

Following gene: 126173164

Centisome position: 20.49

GC content: 50.89

Gene sequence:

>1299_bases
ATGAAGCTTGAATCACTGGCATTACACTACGGTTATGAGTCAGAAGCGACCACAAAAGCGGCCGCGGTACCTATCTATCA
AACCACCTCTTATACCTTCGATGATACCCAGCATGGTGCTGATTTATTTGACTTAAAAGTCGCAGGTAACATTTATACGC
GGATCATGAATCCTACTACTAGCGTGCTAGAGCAGCGTCTCGCAGCCATTGAAGGCGGCATAGGTGCGCTGGCCGTGGCA
TCGGGTATGGCGGCGATTACCTATGCTATTCAAGCCCTGACTCAAGTTGGCGATAATATTGTCAGCACCAGTCAGCTCTA
TGGCGGCACTTACAATTTGTTTGCCCATACGCTGCCGCGCCAAGGCGTAGAAGTGCGTATGGCGGCCTTCGATGATTTTG
AAGGCCTCGATGAGCTGATCGACGACAAAACTAAGGCCTTGTTCTGCGAATCGATTGGTAATCCCGCGGGCAATATTGTC
GATTTACAGCGTTTAGCCGAGATTGCCCATAAGCATGGCGTGCCTTTAATTGTCGATAATACGGTCGCAACCCCAGTGTT
ATGTCGCCCGTTTGAGCATGGCGCCGATATTGTTATCCACTCTTTAACCAAATACATAGGTGGCCATGGCACGACGATTG
GCGGGGTGATTATCGATTCTGGCAAGTTCGATTGGGTGGCCAATAAAGAAAGATTTGCCTTACTCAACCAAGCCGATCCT
TCTTACCACGGTGTGGTTTACACAGAAGCCTTTGGCCCTGCGGCGTTTATTGGTCGCTGCCGTGTGGTACCGCTACGTAA
TACCGGCGCCGCGCTTTCACCGCACAGCGCATTCTTACTGTTGCAAGGCCTTGAAACTCTAAGCCTTAGAATAGAGCGCC
ATTGCTCGAACGCGCTAGCATTAGCCGAATATTTGGTGTTGCATCCGGCGGTGAGTTGGGTGAATTACGGCGCGTTGCCG
AGCAGCCCTTATCAGGATAATTGCCACAAGATCACTGGCGGAAAAGCTTCTGGGATCATCAGCTTTGGGATTAAAGCGGC
CACGCCTGAAGAAGGTAAAATTGCCGGTGGACGTTTTATCGATGCGCTGCAGATGATCCTGCGTTTAGTGAACATCGGCG
ATGCGAAATCCTTGGCTTGTCACCCTGCGAGTACCACGCACAGACAGCTCGATGCCGATGAACTGGCAAGGGCGGGCGTG
TCTGAAGATCTTATCCGTATCTCAGTTGGGATTGAGCATATCGACGACATCATTAACGATGTGGCGCAGGCACTCGATAA
GGCACTGGTTAGCAAGTAG

Upstream 100 bases:

>100_bases
AGACTTCCAGATGTCTATCTGTTAGCTTGTTGAACATGCACAAATCTTGTGCAATCCTTTGGGTTCGAATAAGGACTGCA
CTCTGTTAAAGGAAGGCAAA

Downstream 100 bases:

>100_bases
TTCTTATTAAGTAGCCCATGTCGAATAGCTGATACCCCATCGATTTATTAAATCGTTAACAGTAAAAAGCCGCCATCGAT
TCAATTCGATGGCGGCTTTT

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MKLESLALHYGYESEATTKAAAVPIYQTTSYTFDDTQHGADLFDLKVAGNIYTRIMNPTTSVLEQRLAAIEGGIGALAVA
SGMAAITYAIQALTQVGDNIVSTSQLYGGTYNLFAHTLPRQGVEVRMAAFDDFEGLDELIDDKTKALFCESIGNPAGNIV
DLQRLAEIAHKHGVPLIVDNTVATPVLCRPFEHGADIVIHSLTKYIGGHGTTIGGVIIDSGKFDWVANKERFALLNQADP
SYHGVVYTEAFGPAAFIGRCRVVPLRNTGAALSPHSAFLLLQGLETLSLRIERHCSNALALAEYLVLHPAVSWVNYGALP
SSPYQDNCHKITGGKASGIISFGIKAATPEEGKIAGGRFIDALQMILRLVNIGDAKSLACHPASTTHRQLDADELARAGV
SEDLIRISVGIEHIDDIINDVAQALDKALVSK

Sequences:

>Translated_432_residues
MKLESLALHYGYESEATTKAAAVPIYQTTSYTFDDTQHGADLFDLKVAGNIYTRIMNPTTSVLEQRLAAIEGGIGALAVA
SGMAAITYAIQALTQVGDNIVSTSQLYGGTYNLFAHTLPRQGVEVRMAAFDDFEGLDELIDDKTKALFCESIGNPAGNIV
DLQRLAEIAHKHGVPLIVDNTVATPVLCRPFEHGADIVIHSLTKYIGGHGTTIGGVIIDSGKFDWVANKERFALLNQADP
SYHGVVYTEAFGPAAFIGRCRVVPLRNTGAALSPHSAFLLLQGLETLSLRIERHCSNALALAEYLVLHPAVSWVNYGALP
SSPYQDNCHKITGGKASGIISFGIKAATPEEGKIAGGRFIDALQMILRLVNIGDAKSLACHPASTTHRQLDADELARAGV
SEDLIRISVGIEHIDDIINDVAQALDKALVSK
>Mature_432_residues
MKLESLALHYGYESEATTKAAAVPIYQTTSYTFDDTQHGADLFDLKVAGNIYTRIMNPTTSVLEQRLAAIEGGIGALAVA
SGMAAITYAIQALTQVGDNIVSTSQLYGGTYNLFAHTLPRQGVEVRMAAFDDFEGLDELIDDKTKALFCESIGNPAGNIV
DLQRLAEIAHKHGVPLIVDNTVATPVLCRPFEHGADIVIHSLTKYIGGHGTTIGGVIIDSGKFDWVANKERFALLNQADP
SYHGVVYTEAFGPAAFIGRCRVVPLRNTGAALSPHSAFLLLQGLETLSLRIERHCSNALALAEYLVLHPAVSWVNYGALP
SSPYQDNCHKITGGKASGIISFGIKAATPEEGKIAGGRFIDALQMILRLVNIGDAKSLACHPASTTHRQLDADELARAGV
SEDLIRISVGIEHIDDIINDVAQALDKALVSK

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=424, Percent_Identity=32.311320754717, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI299473759, Length=424, Percent_Identity=28.7735849056604, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI25453487, Length=423, Percent_Identity=28.6052009456265, Blast_Score=125, Evalue=9e-29,
Organism=Escherichia coli, GI1790375, Length=430, Percent_Identity=30.4651162790698, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1789383, Length=430, Percent_Identity=25.5813953488372, Blast_Score=108, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI71980708, Length=415, Percent_Identity=33.4939759036145, Blast_Score=199, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI17538055, Length=429, Percent_Identity=33.7995337995338, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17560056, Length=429, Percent_Identity=33.3333333333333, Blast_Score=179, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6323333, Length=438, Percent_Identity=47.945205479452, Blast_Score=379, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6319307, Length=385, Percent_Identity=33.5064935064935, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6321254, Length=449, Percent_Identity=26.5033407572383, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6321170, Length=311, Percent_Identity=29.2604501607717, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6321904, Length=433, Percent_Identity=26.7898383371824, Blast_Score=98, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19922554, Length=432, Percent_Identity=32.1759259259259, Blast_Score=172, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46231; Mature: 46231

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLESLALHYGYESEATTKAAAVPIYQTTSYTFDDTQHGADLFDLKVAGNIYTRIMNPTT
CCCHHEEEECCCCCCCCCHHHEEEEEECCCCCCCCCCCCCCEEEEEECCCHHHHHCCCHH
SVLEQRLAAIEGGIGALAVASGMAAITYAIQALTQVGDNIVSTSQLYGGTYNLFAHTLPR
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHCCC
QGVEVRMAAFDDFEGLDELIDDKTKALFCESIGNPAGNIVDLQRLAEIAHKHGVPLIVDN
CCCEEEEEECCCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEECC
TVATPVLCRPFEHGADIVIHSLTKYIGGHGTTIGGVIIDSGKFDWVANKERFALLNQADP
CCCCCCEECCCCCCHHHHHHHHHHHHCCCCCEECEEEEECCCCCEEECCCCEEEEECCCC
SYHGVVYTEAFGPAAFIGRCRVVPLRNTGAALSPHSAFLLLQGLETLSLRIERHCSNALA
CCCEEEEECCCCCHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LAEYLVLHPAVSWVNYGALPSSPYQDNCHKITGGKASGIISFGIKAATPEEGKIAGGRFI
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCEEEECEEECCCCCCCCCCHHHH
DALQMILRLVNIGDAKSLACHPASTTHRQLDADELARAGVSEDLIRISVGIEHIDDIIND
HHHHHHHHHHCCCCCCCEEECCCCCCHHCCCHHHHHHCCCCHHHEEEECCHHHHHHHHHH
VAQALDKALVSK
HHHHHHHHHHCC
>Mature Secondary Structure
MKLESLALHYGYESEATTKAAAVPIYQTTSYTFDDTQHGADLFDLKVAGNIYTRIMNPTT
CCCHHEEEECCCCCCCCCHHHEEEEEECCCCCCCCCCCCCCEEEEEECCCHHHHHCCCHH
SVLEQRLAAIEGGIGALAVASGMAAITYAIQALTQVGDNIVSTSQLYGGTYNLFAHTLPR
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHCCC
QGVEVRMAAFDDFEGLDELIDDKTKALFCESIGNPAGNIVDLQRLAEIAHKHGVPLIVDN
CCCEEEEEECCCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEECC
TVATPVLCRPFEHGADIVIHSLTKYIGGHGTTIGGVIIDSGKFDWVANKERFALLNQADP
CCCCCCEECCCCCCHHHHHHHHHHHHCCCCCEECEEEEECCCCCEEECCCCEEEEECCCC
SYHGVVYTEAFGPAAFIGRCRVVPLRNTGAALSPHSAFLLLQGLETLSLRIERHCSNALA
CCCEEEEECCCCCHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LAEYLVLHPAVSWVNYGALPSSPYQDNCHKITGGKASGIISFGIKAATPEEGKIAGGRFI
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCEEEECEEECCCCCCCCCCHHHH
DALQMILRLVNIGDAKSLACHPASTTHRQLDADELARAGVSEDLIRISVGIEHIDDIIND
HHHHHHHHHHCCCCCCCEEECCCCCCHHCCCHHHHHHCCCCHHHEEEECCHHHHHHHHHH
VAQALDKALVSK
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]