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The map label for this gene is prfA

Identifier: 126172944

GI number: 126172944

Start: 790631

End: 791722

Strand: Direct

Name: prfA

Synonym: Sbal_0696

Alternate gene names: 126172944

Gene position: 790631-791722 (Clockwise)

Preceding gene: 126172943

Following gene: 126172945

Centisome position: 15.42

GC content: 50.46

Gene sequence:

>1092_bases
ATGAAGGAATCCGTTATCCGCAAGCTGGAAGGCTTGCTCGAGCGCAATGAAGAAGTCATGGCTTTGCTGGGTGATGCTAG
CGTCATTTCGGATCAAGACAGGTTTCGCGCGTTATCGAAAGAATATGCCCAGTTAGAAGATGTTGTTGCTGGCTTTAAAG
CCTATCAGCAAGCGCAGGTCGATCTCGATTCTGCCAAAGAAATGCTGGAAGAAGACGATGCTGAAATGCGCGAAATGGCG
CAGGAAGAGATGAAAGCCGCTAAGGCTAAGCTTGAGCATCTTGAAGATGAACTGCAAATTCTATTATTGCCAAAAGATCC
CGACGACGATAAAAACGCCTTCGTTGAGATCCGTGCTGGCGCAGGCGGCGATGAAGCGGCGATTTTTGCCGGTGACTTAT
TCCGCATGTATAGCCGTTATGCCGAAGCAAATCGCTGGCAGATCGAAATCATGAGTTGTAACGAAGGCGAACATGGTGGC
TTCAAAGAAGTGATCATGAAGGTGAGTGGCGACGGCGTATATGGCAAACTTAAGTTTGAGTCTGGCGGTCACCGCGTACA
ACGTGTGCCAGAAACCGAATCTCAAGGCCGTGTGCATACTTCAGCGGTTACTGTGGTTGTATTGCATGAAGTGCCTGAAG
CAGAAGCGATTTCAATTAACCCAGCCGATCTGAAAGTTGATACTTTCCGTTCATCGGGCGCGGGTGGTCAGCACGTTAAC
AAAACCGATTCTGCCATTCGTATAACCCACATTCCAACGGGGATCGTGGTGGAATGCCAAGATCAACGCTCGCAACATAA
GAACCGTGCCCAAGCCATGAGTGTACTGGCCGCACGTATTCAAGCTCTTGAAGACGAAAAACGCCGTAGCGCCGAAGAAT
CGACCCGTCGTAGTCTGGTTGCCAGTGGCGATCGCTCCGAACGTGTGCGTACTTACAACTTCCCGCAAGGCCGCGTGAGC
GAACACCGCATCAACTTGACCTTATATCGCTTGAACGAAGTGATGGAAGGCGATCTCGATGCTATTTTATTGCCTCTGAT
GCAAGAGCATCAAGCCGATCAGTTAGCCGCACTGGCTGACGAGCAGGGGTAG

Upstream 100 bases:

>100_bases
CACAGGCCCTCACGGCAGCGAGTCGTCAGGGCGATTTGAATACTCTCGGTCAGTTAAGATCAGCGCTTGGATTAGATAAA
AACTAAGGTTAGCGAGTTAA

Downstream 100 bases:

>100_bases
TTTTGTCAGATCAATCTAGCATAGCCGAAGCCCTGCAATGGGCTTATGTGCAGTTAGCATCAACCTCTGAATCCGCTCAT
CTGGATGCAGAGGTTTTGTT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MKESVIRKLEGLLERNEEVMALLGDASVISDQDRFRALSKEYAQLEDVVAGFKAYQQAQVDLDSAKEMLEEDDAEMREMA
QEEMKAAKAKLEHLEDELQILLLPKDPDDDKNAFVEIRAGAGGDEAAIFAGDLFRMYSRYAEANRWQIEIMSCNEGEHGG
FKEVIMKVSGDGVYGKLKFESGGHRVQRVPETESQGRVHTSAVTVVVLHEVPEAEAISINPADLKVDTFRSSGAGGQHVN
KTDSAIRITHIPTGIVVECQDQRSQHKNRAQAMSVLAARIQALEDEKRRSAEESTRRSLVASGDRSERVRTYNFPQGRVS
EHRINLTLYRLNEVMEGDLDAILLPLMQEHQADQLAALADEQG

Sequences:

>Translated_363_residues
MKESVIRKLEGLLERNEEVMALLGDASVISDQDRFRALSKEYAQLEDVVAGFKAYQQAQVDLDSAKEMLEEDDAEMREMA
QEEMKAAKAKLEHLEDELQILLLPKDPDDDKNAFVEIRAGAGGDEAAIFAGDLFRMYSRYAEANRWQIEIMSCNEGEHGG
FKEVIMKVSGDGVYGKLKFESGGHRVQRVPETESQGRVHTSAVTVVVLHEVPEAEAISINPADLKVDTFRSSGAGGQHVN
KTDSAIRITHIPTGIVVECQDQRSQHKNRAQAMSVLAARIQALEDEKRRSAEESTRRSLVASGDRSERVRTYNFPQGRVS
EHRINLTLYRLNEVMEGDLDAILLPLMQEHQADQLAALADEQG
>Mature_363_residues
MKESVIRKLEGLLERNEEVMALLGDASVISDQDRFRALSKEYAQLEDVVAGFKAYQQAQVDLDSAKEMLEEDDAEMREMA
QEEMKAAKAKLEHLEDELQILLLPKDPDDDKNAFVEIRAGAGGDEAAIFAGDLFRMYSRYAEANRWQIEIMSCNEGEHGG
FKEVIMKVSGDGVYGKLKFESGGHRVQRVPETESQGRVHTSAVTVVVLHEVPEAEAISINPADLKVDTFRSSGAGGQHVN
KTDSAIRITHIPTGIVVECQDQRSQHKNRAQAMSVLAARIQALEDEKRRSAEESTRRSLVASGDRSERVRTYNFPQGRVS
EHRINLTLYRLNEVMEGDLDAILLPLMQEHQADQLAALADEQG

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=292, Percent_Identity=41.7808219178082, Blast_Score=261, Evalue=6e-70,
Organism=Homo sapiens, GI34577120, Length=346, Percent_Identity=39.8843930635838, Blast_Score=246, Evalue=3e-65,
Organism=Homo sapiens, GI166795305, Length=207, Percent_Identity=40.0966183574879, Blast_Score=180, Evalue=2e-45,
Organism=Escherichia coli, GI1787462, Length=362, Percent_Identity=71.8232044198895, Blast_Score=531, Evalue=1e-152,
Organism=Escherichia coli, GI2367172, Length=333, Percent_Identity=39.6396396396396, Blast_Score=217, Evalue=8e-58,
Organism=Caenorhabditis elegans, GI17542784, Length=336, Percent_Identity=33.9285714285714, Blast_Score=183, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6321295, Length=313, Percent_Identity=42.8115015974441, Blast_Score=249, Evalue=4e-67,
Organism=Drosophila melanogaster, GI19921226, Length=331, Percent_Identity=39.8791540785499, Blast_Score=218, Evalue=8e-57,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_SHEB2 (B8E815)

Other databases:

- EMBL:   CP001252
- RefSeq:   YP_002359451.1
- ProteinModelPortal:   B8E815
- SMR:   B8E815
- GeneID:   7088659
- GenomeReviews:   CP001252_GR
- KEGG:   sbp:Sbal223_3546
- HOGENOM:   HBG629764
- OMA:   NTNEGEH
- ProtClustDB:   PRK00591
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40506; Mature: 40506

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKESVIRKLEGLLERNEEVMALLGDASVISDQDRFRALSKEYAQLEDVVAGFKAYQQAQV
CCHHHHHHHHHHHHCCHHHEEEECCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DLDSAKEMLEEDDAEMREMAQEEMKAAKAKLEHLEDELQILLLPKDPDDDKNAFVEIRAG
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEEEEC
AGGDEAAIFAGDLFRMYSRYAEANRWQIEIMSCNEGEHGGFKEVIMKVSGDGVYGKLKFE
CCCCCCEEHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHCCCCCEEEEEEEC
SGGHRVQRVPETESQGRVHTSAVTVVVLHEVPEAEAISINPADLKVDTFRSSGAGGQHVN
CCCCCEEECCCCCCCCCEEEEEEEEEEEECCCCCCEEECCCCCEEEEECCCCCCCCCCCC
KTDSAIRITHIPTGIVVECQDQRSQHKNRAQAMSVLAARIQALEDEKRRSAEESTRRSLV
CCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASGDRSERVRTYNFPQGRVSEHRINLTLYRLNEVMEGDLDAILLPLMQEHQADQLAALAD
HCCCCCCCCEEECCCCCCCCCEEEEEEEEEHHHHHHCCHHHHHHHHHHHCCHHHHHHHHC
EQG
CCC
>Mature Secondary Structure
MKESVIRKLEGLLERNEEVMALLGDASVISDQDRFRALSKEYAQLEDVVAGFKAYQQAQV
CCHHHHHHHHHHHHCCHHHEEEECCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DLDSAKEMLEEDDAEMREMAQEEMKAAKAKLEHLEDELQILLLPKDPDDDKNAFVEIRAG
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEEEEC
AGGDEAAIFAGDLFRMYSRYAEANRWQIEIMSCNEGEHGGFKEVIMKVSGDGVYGKLKFE
CCCCCCEEHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHCCCCCEEEEEEEC
SGGHRVQRVPETESQGRVHTSAVTVVVLHEVPEAEAISINPADLKVDTFRSSGAGGQHVN
CCCCCEEECCCCCCCCCEEEEEEEEEEEECCCCCCEEECCCCCEEEEECCCCCCCCCCCC
KTDSAIRITHIPTGIVVECQDQRSQHKNRAQAMSVLAARIQALEDEKRRSAEESTRRSLV
CCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASGDRSERVRTYNFPQGRVSEHRINLTLYRLNEVMEGDLDAILLPLMQEHQADQLAALAD
HCCCCCCCCEEECCCCCCCCCEEEEEEEEEHHHHHHCCHHHHHHHHHHHCCHHHHHHHHC
EQG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA