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The map label for this gene is hemK [H]

Identifier: 126172945

GI number: 126172945

Start: 791725

End: 792573

Strand: Direct

Name: hemK [H]

Synonym: Sbal_0697

Alternate gene names: 126172945

Gene position: 791725-792573 (Clockwise)

Preceding gene: 126172944

Following gene: 126172948

Centisome position: 15.44

GC content: 48.88

Gene sequence:

>849_bases
TTGTCAGATCAATCTAGCATAGCCGAAGCCCTGCAATGGGCTTATGTGCAGTTAGCATCAACCTCTGAATCCGCTCATCT
GGATGCAGAGGTTTTGTTGTTATATTGCCTCAATAAAAGTCGCAGCTATTTATATACTTGGCCTGAAAAATCCCTGACGG
TTGAGCAGTGGAAACGCTTCCAGCAAATGGTACAGAAACGCCAAAAGGGCGTGCCTGTGGCACATATTGTCGGTGAGCGC
GAATTTTGGTCACTGCCTTTTATCGTCAACGACACGACTTTGATCCCGCGTCCTGATACGGAAATTTTAGTCGAAACCGC
CTTGAACATGCCGCTTTCTACCTACGCTAACGTGCTCGACTTAGGTACAGGCACTGGGGCCATTGCGCTGGCGTTGGCGT
CTGAGCGTGAGTCTTGGCAAATCACGGCGGTCGATAAAGTCGATGATGCCGTGGCTTTGGCTATCGCGAACCGTGAAAAC
TTAAAGTTGCCACAGGTCGATATTCTTCAAAGTGACTGGTTTAGCGCGGTAAAGTCGCGTGACTTTGACTTAATCGTCTC
AAATCCGCCTTATATTGATGAGACTGACGAACATTTGCACCAAGGTGACGTGCGTTTCGAGCCCCAAAGCGCGTTAACCG
CCGCCGAGGAAGGCTTTGCCGATCTGTATTATATTGCCAACACTGCCCGAGATTACTTAAAGCCTAACGGCTATATCCTG
CTCGAGCATGGCTTCGAACAGGCGGTAAGACTCAGAGCTAAGCTTATCGAACTGGGCTATGAAAATGTCGCCACAGTGCG
AGACTTTGGCAGCAATGACAGATGTACTATGGGTAAATGGGCTGAATAA

Upstream 100 bases:

>100_bases
ACGAAGTGATGGAAGGCGATCTCGATGCTATTTTATTGCCTCTGATGCAAGAGCATCAAGCCGATCAGTTAGCCGCACTG
GCTGACGAGCAGGGGTAGTT

Downstream 100 bases:

>100_bases
ATTTTAAAAATAAATTCTATGTTTCAATAGAACAATGATGAGCAGTTAGACATATTACCTTGCTACCTTAAATATAACGC
TATTGCTAACTTGCAATGGC

Product: HemK family modification methylase

Products: NA

Alternate protein names: M.EcoKHemKP; Protein-(glutamine-N(5)) MTase hemK; Protein-glutamine N-methyltransferase hemK [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MSDQSSIAEALQWAYVQLASTSESAHLDAEVLLLYCLNKSRSYLYTWPEKSLTVEQWKRFQQMVQKRQKGVPVAHIVGER
EFWSLPFIVNDTTLIPRPDTEILVETALNMPLSTYANVLDLGTGTGAIALALASERESWQITAVDKVDDAVALAIANREN
LKLPQVDILQSDWFSAVKSRDFDLIVSNPPYIDETDEHLHQGDVRFEPQSALTAAEEGFADLYYIANTARDYLKPNGYIL
LEHGFEQAVRLRAKLIELGYENVATVRDFGSNDRCTMGKWAE

Sequences:

>Translated_282_residues
MSDQSSIAEALQWAYVQLASTSESAHLDAEVLLLYCLNKSRSYLYTWPEKSLTVEQWKRFQQMVQKRQKGVPVAHIVGER
EFWSLPFIVNDTTLIPRPDTEILVETALNMPLSTYANVLDLGTGTGAIALALASERESWQITAVDKVDDAVALAIANREN
LKLPQVDILQSDWFSAVKSRDFDLIVSNPPYIDETDEHLHQGDVRFEPQSALTAAEEGFADLYYIANTARDYLKPNGYIL
LEHGFEQAVRLRAKLIELGYENVATVRDFGSNDRCTMGKWAE
>Mature_281_residues
SDQSSIAEALQWAYVQLASTSESAHLDAEVLLLYCLNKSRSYLYTWPEKSLTVEQWKRFQQMVQKRQKGVPVAHIVGERE
FWSLPFIVNDTTLIPRPDTEILVETALNMPLSTYANVLDLGTGTGAIALALASERESWQITAVDKVDDAVALAIANRENL
KLPQVDILQSDWFSAVKSRDFDLIVSNPPYIDETDEHLHQGDVRFEPQSALTAAEEGFADLYYIANTARDYLKPNGYILL
EHGFEQAVRLRAKLIELGYENVATVRDFGSNDRCTMGKWAE

Specific function: Methylates the translation termination release factor RF1/PrfA on 'Gln-235' and RF2/PrfB on 'Gln-252' [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=236, Percent_Identity=31.7796610169492, Blast_Score=89, Evalue=4e-18,
Organism=Escherichia coli, GI1787463, Length=270, Percent_Identity=50.3703703703704, Blast_Score=273, Evalue=9e-75,
Organism=Escherichia coli, GI87082085, Length=192, Percent_Identity=30.2083333333333, Blast_Score=90, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324266, Length=199, Percent_Identity=29.6482412060301, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24582226, Length=264, Percent_Identity=25, Blast_Score=88, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 31805; Mature: 31674

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDQSSIAEALQWAYVQLASTSESAHLDAEVLLLYCLNKSRSYLYTWPEKSLTVEQWKRF
CCCHHHHHHHHHHHHHHEECCCCCCCCHHHHHHEEHHCCCCCEEEECCCCCCCHHHHHHH
QQMVQKRQKGVPVAHIVGEREFWSLPFIVNDTTLIPRPDTEILVETALNMPLSTYANVLD
HHHHHHHHCCCCEEEEECCCHHEECCEEECCCEECCCCCHHHHHHHHHCCCHHHHHHHHH
LGTGTGAIALALASERESWQITAVDKVDDAVALAIANRENLKLPQVDILQSDWFSAVKSR
CCCCCCEEEEEEECCCCCEEEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCC
DFDLIVSNPPYIDETDEHLHQGDVRFEPQSALTAAEEGFADLYYIANTARDYLKPNGYIL
CCEEEECCCCCCCCCHHHHHCCCCEECCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEE
LEHGFEQAVRLRAKLIELGYENVATVRDFGSNDRCTMGKWAE
EECCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDQSSIAEALQWAYVQLASTSESAHLDAEVLLLYCLNKSRSYLYTWPEKSLTVEQWKRF
CCHHHHHHHHHHHHHHEECCCCCCCCHHHHHHEEHHCCCCCEEEECCCCCCCHHHHHHH
QQMVQKRQKGVPVAHIVGEREFWSLPFIVNDTTLIPRPDTEILVETALNMPLSTYANVLD
HHHHHHHHCCCCEEEEECCCHHEECCEEECCCEECCCCCHHHHHHHHHCCCHHHHHHHHH
LGTGTGAIALALASERESWQITAVDKVDDAVALAIANRENLKLPQVDILQSDWFSAVKSR
CCCCCCEEEEEEECCCCCEEEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCC
DFDLIVSNPPYIDETDEHLHQGDVRFEPQSALTAAEEGFADLYYIANTARDYLKPNGYIL
CCEEEECCCCCCCCCHHHHHCCCCEECCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEE
LEHGFEQAVRLRAKLIELGYENVATVRDFGSNDRCTMGKWAE
EECCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7883187; 7543480; 11805295; 8905232; 9278503 [H]