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The map label for this gene is dacA [H]

Identifier: 125625313

GI number: 125625313

Start: 2519311

End: 2520615

Strand: Direct

Name: dacA [H]

Synonym: llmg_2560

Alternate gene names: 125625313

Gene position: 2519311-2520615 (Clockwise)

Preceding gene: 125625303

Following gene: NA

Centisome position: 99.6

GC content: 35.86

Gene sequence:

>1305_bases
ATGAAAAAAATTGCAATTATTTTTTGTACTTTACTGATGAGTCTATCAGTCCTTTCGAGCTTTGCTGTCAGTGCTGATAC
TACTACAACAAATAACTTTACAGTTGCCGCAAAATCAGCAATTGCTATTGACGCCTCAAGTGGTAAAATTTTATACGCGC
AAAATGCTGACGATGCAAGCACACGAATTGCTTCTATCACAAAATTACTGACGGCTTATTTAGTTTACAAAAATGTAGCT
GAGAATAAATTAACTTGGACTACTAAGGTTCCTATTTCAGACTATGCTTATGAATTAACTCAAAATTCTAATGCTTCCAA
CATTCCGCTAGCTCAAAATGGTCAGTATACTGTCAAAGATTTATTGAATGCGTTGCTTCTTCCATCCGCAAATTCTGCTG
CTATCGCTCTAGCTGAAAAAATTGCTGGCTCAGAACCAAAATTTGTTGATATGATGACAAAACAAATGAAAGATTGGGGA
ATTACTGACAGCCATCTCGTCAATGCTTCCGGCTTACCAAATGATGATTTGAATGGTCATATTTATCCAGGTTCTGGCGC
CTCAGCAACAAATACAATGAGTGCAAAAGCCGTTGCAACACTCAGTTATCACTTAATTCAAGATTTTCCAGAAGTTCTTC
AAATTACTAAGAAAACAGAAATTCCTTTTGATACAGGTAATGAAGAAAAAATGACTTTAACCAATACCAACCAAATGCTT
AGTGGATTTTCTACTTCAAGAACTGGTGTAGATGGTTTAAAAACGGGGTCAACTAGTTTCCAAATTGACTGTTTTGCTGG
AACAACCAATCAAAATGGTTTCAGAATTATCACAGTTGTTTTAGAAGCCACAGATCCTGCCGCTGATAACTCAACACCGT
TTACCCTAACAAATCAGCTAATGAACTATGTTTACGGGCATTGGCGTACAACCAACCTTGTACAAAAAAATCAATCTTTA
GATGATTTTAAAGAAATTGCTGTGACTGATGGAAAAGAAAAATCAGTTCAACTTGTTGCGCCACAAAATGTCACCCCTGT
TGTTCCCTACGCTACAGATGGTAGTGCTGACACGAAACCATTAGTAATCAAGTTCGATAAGAAAAAGAATGCCACAGTTG
AAGCAACAATCACTAAAAATCAAAAATTAGTCACTCTTTCTACTACAGTGAAAGATAGTTTAGGCTATCTTCCAAATTGT
ACAGCAGAGAAGATTCCTTTAGTTGCTAAAAAAACAGTACCACGTAGTAGTGCTCCAAAAGTTTTTTGGAATCATTTTGT
TAATTTTGTTAATGAAAAACTCTAA

Upstream 100 bases:

>100_bases
GCTAGAGTTTTTCTAGCTTTTTACTTGTGGGTTTAAAAAGTGCTGATACATTATTTGAGGGCTATTTGTATCTCTTAACT
CACTATGTTATAATCAAATC

Downstream 100 bases:

>100_bases
AATTTACTGACAGAAAAATTGGTCAGTAAAATTAGCGAATAAAAAAGCTGTCTATTTACTGACAGCTTTTTTTATTAACC
TTTAGATTTCACAATCGCAA

Product: DacA protein

Products: NA

Alternate protein names: CPase; DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MKKIAIIFCTLLMSLSVLSSFAVSADTTTTNNFTVAAKSAIAIDASSGKILYAQNADDASTRIASITKLLTAYLVYKNVA
ENKLTWTTKVPISDYAYELTQNSNASNIPLAQNGQYTVKDLLNALLLPSANSAAIALAEKIAGSEPKFVDMMTKQMKDWG
ITDSHLVNASGLPNDDLNGHIYPGSGASATNTMSAKAVATLSYHLIQDFPEVLQITKKTEIPFDTGNEEKMTLTNTNQML
SGFSTSRTGVDGLKTGSTSFQIDCFAGTTNQNGFRIITVVLEATDPAADNSTPFTLTNQLMNYVYGHWRTTNLVQKNQSL
DDFKEIAVTDGKEKSVQLVAPQNVTPVVPYATDGSADTKPLVIKFDKKKNATVEATITKNQKLVTLSTTVKDSLGYLPNC
TAEKIPLVAKKTVPRSSAPKVFWNHFVNFVNEKL

Sequences:

>Translated_434_residues
MKKIAIIFCTLLMSLSVLSSFAVSADTTTTNNFTVAAKSAIAIDASSGKILYAQNADDASTRIASITKLLTAYLVYKNVA
ENKLTWTTKVPISDYAYELTQNSNASNIPLAQNGQYTVKDLLNALLLPSANSAAIALAEKIAGSEPKFVDMMTKQMKDWG
ITDSHLVNASGLPNDDLNGHIYPGSGASATNTMSAKAVATLSYHLIQDFPEVLQITKKTEIPFDTGNEEKMTLTNTNQML
SGFSTSRTGVDGLKTGSTSFQIDCFAGTTNQNGFRIITVVLEATDPAADNSTPFTLTNQLMNYVYGHWRTTNLVQKNQSL
DDFKEIAVTDGKEKSVQLVAPQNVTPVVPYATDGSADTKPLVIKFDKKKNATVEATITKNQKLVTLSTTVKDSLGYLPNC
TAEKIPLVAKKTVPRSSAPKVFWNHFVNFVNEKL
>Mature_434_residues
MKKIAIIFCTLLMSLSVLSSFAVSADTTTTNNFTVAAKSAIAIDASSGKILYAQNADDASTRIASITKLLTAYLVYKNVA
ENKLTWTTKVPISDYAYELTQNSNASNIPLAQNGQYTVKDLLNALLLPSANSAAIALAEKIAGSEPKFVDMMTKQMKDWG
ITDSHLVNASGLPNDDLNGHIYPGSGASATNTMSAKAVATLSYHLIQDFPEVLQITKKTEIPFDTGNEEKMTLTNTNQML
SGFSTSRTGVDGLKTGSTSFQIDCFAGTTNQNGFRIITVVLEATDPAADNSTPFTLTNQLMNYVYGHWRTTNLVQKNQSL
DDFKEIAVTDGKEKSVQLVAPQNVTPVVPYATDGSADTKPLVIKFDKKKNATVEATITKNQKLVTLSTTVKDSLGYLPNC
TAEKIPLVAKKTVPRSSAPKVFWNHFVNFVNEKL

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=286, Percent_Identity=30.7692307692308, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87082022, Length=371, Percent_Identity=25.3369272237197, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1786851, Length=435, Percent_Identity=23.9080459770115, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 46984; Mature: 46984

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIAIIFCTLLMSLSVLSSFAVSADTTTTNNFTVAAKSAIAIDASSGKILYAQNADDAS
CCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEEEEEEEEEECCCCEEEEEECCCCHH
TRIASITKLLTAYLVYKNVAENKLTWTTKVPISDYAYELTQNSNASNIPLAQNGQYTVKD
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHEEECCCCCCCCCCCCCCCCCHHHH
LLNALLLPSANSAAIALAEKIAGSEPKFVDMMTKQMKDWGITDSHLVNASGLPNDDLNGH
HHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHEEECCCCCCCCCCCE
IYPGSGASATNTMSAKAVATLSYHLIQDFPEVLQITKKTEIPFDTGNEEKMTLTNTNQML
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHH
SGFSTSRTGVDGLKTGSTSFQIDCFAGTTNQNGFRIITVVLEATDPAADNSTPFTLTNQL
HCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHH
MNYVYGHWRTTNLVQKNQSLDDFKEIAVTDGKEKSVQLVAPQNVTPVVPYATDGSADTKP
HHHHHCCCCCCHHHHCCCCHHHHHHHHCCCCCCCCEEEECCCCCCCEEEECCCCCCCCCC
LVIKFDKKKNATVEATITKNQKLVTLSTTVKDSLGYLPNCTAEKIPLVAKKTVPRSSAPK
EEEEECCCCCCEEEEEEECCCEEEEEEECHHHHCCCCCCCCHHHCCEEEECCCCCCCCCH
VFWNHFVNFVNEKL
HHHHHHHHHHHHCC
>Mature Secondary Structure
MKKIAIIFCTLLMSLSVLSSFAVSADTTTTNNFTVAAKSAIAIDASSGKILYAQNADDAS
CCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEEEEEEEEEECCCCEEEEEECCCCHH
TRIASITKLLTAYLVYKNVAENKLTWTTKVPISDYAYELTQNSNASNIPLAQNGQYTVKD
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHEEECCCCCCCCCCCCCCCCCHHHH
LLNALLLPSANSAAIALAEKIAGSEPKFVDMMTKQMKDWGITDSHLVNASGLPNDDLNGH
HHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHEEECCCCCCCCCCCE
IYPGSGASATNTMSAKAVATLSYHLIQDFPEVLQITKKTEIPFDTGNEEKMTLTNTNQML
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHH
SGFSTSRTGVDGLKTGSTSFQIDCFAGTTNQNGFRIITVVLEATDPAADNSTPFTLTNQL
HCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHH
MNYVYGHWRTTNLVQKNQSLDDFKEIAVTDGKEKSVQLVAPQNVTPVVPYATDGSADTKP
HHHHHCCCCCCHHHHCCCCHHHHHHHHCCCCCCCCEEEECCCCCCCEEEECCCCCCCCCC
LVIKFDKKKNATVEATITKNQKLVTLSTTVKDSLGYLPNCTAEKIPLVAKKTVPRSSAPK
EEEEECCCCCCEEEEEEECCCEEEEEEECHHHHCCCCCCCCHHHCCEEEECCCCCCCCCH
VFWNHFVNFVNEKL
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8370539; 6780559; 111240 [H]