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The map label for this gene is gltX [H]

Identifier: 125625093

GI number: 125625093

Start: 2290608

End: 2292059

Strand: Reverse

Name: gltX [H]

Synonym: llmg_2332

Alternate gene names: 125625093

Gene position: 2292059-2290608 (Counterclockwise)

Preceding gene: 125625094

Following gene: 125625092

Centisome position: 90.61

GC content: 39.19

Gene sequence:

>1452_bases
ATGAACAAAAAAATCCGCGTCCGTTACGCACCATCACCAACAGGGCTTTTGCATATCGGGAACGCCCGTACAGCCCTTTT
TAACTATCTTTTTGCCCGTCATCACGGGGGAGATTTCATCATCCGTATTGAAGATACAGACCGTGAACGCCATGTTGAAG
ATGGTGAACGTTCACAACTTGAAAACCTTCGTTGGTTGGGAATGGATTGGGATGAAAGCCCTGAAACACACGAAAATTAT
CGTCAATCAGAACGCTTGCCTTTGTATCAAAAATATATTGACCAATTGTTAGCTGAGGGTAAAGCTTATTATTCTTACAA
AACTCCTGAAGAACTAGAAGCTGACCATGCTAAACAAGAAGCAGCAGGAATTCCCCCACATTATATCAATGAATATGCTG
GCATGAGCGATGACGAAAAAGCAGCTTATATTGCTGAACGTAAAGCACAAAATATTGAACCTGTCGTTCGTATCTCTGTT
GATGAAAAAGCCATTTACAAATGGAATGACATCGTTAAAGGTGAAATTGAATTTGAAGGTGGCAATATCGGTGGTGATTG
GGTTATCCAAAAACGTGATGGTTACCCAACTTACAACTTTGCTGTTGTTGTTGATGACCACGATATGCAAATTTCACATG
TCATTCGTGGAGACGACCATATTGCCAATACGCCAAAACAATTGGTTGTTTATAATGCTTTAGGTTGGGAAGCTCCACAA
TTTGGTCACATGACTTTGATTATTAATTCTGAAACAGGAAAAAAATTGTCAAAACGTGACACAAACACACTCCAATTTAT
CGAAGACTACCGTAAAAAAGGATATATGTCTGATGCCATTTTCAACTTTATTGCGCTTTTGGGTTGGAATCCTGGTGGAG
AAAAAGAAATTTTCTCTTGTGAAGAATTGATTGAACTTTTTGACGAAAATCGTTTGTCTAAATCACCAGCAGCCTTTGAC
CAAAAGAAATTGGACTGGATGGACAATGAATACATCAAAAATGCCGATTTTGCGAAAGTTTTTGAATTGACAAAACCATT
CCTCGTTGCCGCTAATCGTTTTGATGAACGTGCTAAAGAATTGGTCAAACTTTATCAACCACAAATGAAATCAGCAGATG
AGATTGTTGAATTAACAGAGCTTTTCTACGGAGATTTCCCAGAATTGACTGATGAAGCACGCGAAATGTTAGCCGCTGAA
ACAACACCACTTGTATTAAGTACTTTCCGTGCAAAATTGGCTGAACTTCCTGAAAGTGACTTTACGGTTGAGAATATTTT
CCCACTTTTTAAAGCCACTCAAAAAGAAACAGGCGTGAAAGGTAAAATGCTCTGGATGCCAATCCGTATTGCTGCTTCAG
GCTCAATGCACGGTCCAGAATTACCAGAAACGATTGCTCTTCTTGGAAAAGAAAAGGTATTGGCTCACTTGGATGCCGCT
TTAAATAAATAA

Upstream 100 bases:

>100_bases
TAGATGTGATTGTTAGAGTAGTAATTACTATTTAAAACCAATAAAGATTCATTTCTGATAAAAAAGAAGTGAAGAAATCA
ATGAGGAGAATTGGATTAAA

Downstream 100 bases:

>100_bases
AATAATAAAAAGACGCCTTGTGGCGTTTTTTTATTAAGTCATGATGAACCACACAGACCTTCAAAAGTTATAGAATAGAA
GCATAAAGAAAAGGGAGGCA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MNKKIRVRYAPSPTGLLHIGNARTALFNYLFARHHGGDFIIRIEDTDRERHVEDGERSQLENLRWLGMDWDESPETHENY
RQSERLPLYQKYIDQLLAEGKAYYSYKTPEELEADHAKQEAAGIPPHYINEYAGMSDDEKAAYIAERKAQNIEPVVRISV
DEKAIYKWNDIVKGEIEFEGGNIGGDWVIQKRDGYPTYNFAVVVDDHDMQISHVIRGDDHIANTPKQLVVYNALGWEAPQ
FGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYMSDAIFNFIALLGWNPGGEKEIFSCEELIELFDENRLSKSPAAFD
QKKLDWMDNEYIKNADFAKVFELTKPFLVAANRFDERAKELVKLYQPQMKSADEIVELTELFYGDFPELTDEAREMLAAE
TTPLVLSTFRAKLAELPESDFTVENIFPLFKATQKETGVKGKMLWMPIRIAASGSMHGPELPETIALLGKEKVLAHLDAA
LNK

Sequences:

>Translated_483_residues
MNKKIRVRYAPSPTGLLHIGNARTALFNYLFARHHGGDFIIRIEDTDRERHVEDGERSQLENLRWLGMDWDESPETHENY
RQSERLPLYQKYIDQLLAEGKAYYSYKTPEELEADHAKQEAAGIPPHYINEYAGMSDDEKAAYIAERKAQNIEPVVRISV
DEKAIYKWNDIVKGEIEFEGGNIGGDWVIQKRDGYPTYNFAVVVDDHDMQISHVIRGDDHIANTPKQLVVYNALGWEAPQ
FGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYMSDAIFNFIALLGWNPGGEKEIFSCEELIELFDENRLSKSPAAFD
QKKLDWMDNEYIKNADFAKVFELTKPFLVAANRFDERAKELVKLYQPQMKSADEIVELTELFYGDFPELTDEAREMLAAE
TTPLVLSTFRAKLAELPESDFTVENIFPLFKATQKETGVKGKMLWMPIRIAASGSMHGPELPETIALLGKEKVLAHLDAA
LNK
>Mature_483_residues
MNKKIRVRYAPSPTGLLHIGNARTALFNYLFARHHGGDFIIRIEDTDRERHVEDGERSQLENLRWLGMDWDESPETHENY
RQSERLPLYQKYIDQLLAEGKAYYSYKTPEELEADHAKQEAAGIPPHYINEYAGMSDDEKAAYIAERKAQNIEPVVRISV
DEKAIYKWNDIVKGEIEFEGGNIGGDWVIQKRDGYPTYNFAVVVDDHDMQISHVIRGDDHIANTPKQLVVYNALGWEAPQ
FGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYMSDAIFNFIALLGWNPGGEKEIFSCEELIELFDENRLSKSPAAFD
QKKLDWMDNEYIKNADFAKVFELTKPFLVAANRFDERAKELVKLYQPQMKSADEIVELTELFYGDFPELTDEAREMLAAE
TTPLVLSTFRAKLAELPESDFTVENIFPLFKATQKETGVKGKMLWMPIRIAASGSMHGPELPETIALLGKEKVLAHLDAA
LNK

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=323, Percent_Identity=37.7708978328173, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI62241042, Length=236, Percent_Identity=24.5762711864407, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788743, Length=483, Percent_Identity=37.888198757764, Blast_Score=315, Evalue=5e-87,
Organism=Escherichia coli, GI226510925, Length=310, Percent_Identity=29.0322580645161, Blast_Score=115, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI71993394, Length=490, Percent_Identity=31.8367346938775, Blast_Score=200, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17543332, Length=298, Percent_Identity=24.1610738255034, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324540, Length=484, Percent_Identity=31.6115702479339, Blast_Score=227, Evalue=3e-60,
Organism=Saccharomyces cerevisiae, GI6321192, Length=217, Percent_Identity=26.2672811059908, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324742, Length=313, Percent_Identity=24.2811501597444, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24665321, Length=503, Percent_Identity=29.8210735586481, Blast_Score=219, Evalue=5e-57,
Organism=Drosophila melanogaster, GI17864488, Length=300, Percent_Identity=26, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 55356; Mature: 55356

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKIRVRYAPSPTGLLHIGNARTALFNYLFARHHGGDFIIRIEDTDRERHVEDGERSQL
CCCCEEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCHHCCCCCCHHHH
ENLRWLGMDWDESPETHENYRQSERLPLYQKYIDQLLAEGKAYYSYKTPEELEADHAKQE
HCCEEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHH
AAGIPPHYINEYAGMSDDEKAAYIAERKAQNIEPVVRISVDEKAIYKWNDIVKGEIEFEG
HCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEEECCHHHEECCCEEEEEEEECC
GNIGGDWVIQKRDGYPTYNFAVVVDDHDMQISHVIRGDDHIANTPKQLVVYNALGWEAPQ
CCCCCCEEEEECCCCCCEEEEEEEECCCCEEEEEECCCCHHCCCCCCEEEEECCCCCCCC
FGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYMSDAIFNFIALLGWNPGGEKEIFSC
CCCEEEEEECCCCCHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHH
EELIELFDENRLSKSPAAFDQKKLDWMDNEYIKNADFAKVFELTKPFLVAANRFDERAKE
HHHHHHHHCCCCCCCCCCCCHHHHCCCCCHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHH
LVKLYQPQMKSADEIVELTELFYGDFPELTDEAREMLAAETTPLVLSTFRAKLAELPESD
HHHHHCHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCC
FTVENIFPLFKATQKETGVKGKMLWMPIRIAASGSMHGPELPETIALLGKEKVLAHLDAA
CCHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
LNK
HCC
>Mature Secondary Structure
MNKKIRVRYAPSPTGLLHIGNARTALFNYLFARHHGGDFIIRIEDTDRERHVEDGERSQL
CCCCEEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCHHCCCCCCHHHH
ENLRWLGMDWDESPETHENYRQSERLPLYQKYIDQLLAEGKAYYSYKTPEELEADHAKQE
HCCEEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHH
AAGIPPHYINEYAGMSDDEKAAYIAERKAQNIEPVVRISVDEKAIYKWNDIVKGEIEFEG
HCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEEECCHHHEECCCEEEEEEEECC
GNIGGDWVIQKRDGYPTYNFAVVVDDHDMQISHVIRGDDHIANTPKQLVVYNALGWEAPQ
CCCCCCEEEEECCCCCCEEEEEEEECCCCEEEEEECCCCHHCCCCCCEEEEECCCCCCCC
FGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYMSDAIFNFIALLGWNPGGEKEIFSC
CCCEEEEEECCCCCHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHH
EELIELFDENRLSKSPAAFDQKKLDWMDNEYIKNADFAKVFELTKPFLVAANRFDERAKE
HHHHHHHHCCCCCCCCCCCCHHHHCCCCCHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHH
LVKLYQPQMKSADEIVELTELFYGDFPELTDEAREMLAAETTPLVLSTFRAKLAELPESD
HHHHHCHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCC
FTVENIFPLFKATQKETGVKGKMLWMPIRIAASGSMHGPELPETIALLGKEKVLAHLDAA
CCHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
LNK
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]