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The map label for this gene is ackA2 [H]

Identifier: 125625052

GI number: 125625052

Start: 2241211

End: 2242398

Strand: Reverse

Name: ackA2 [H]

Synonym: llmg_2288

Alternate gene names: 125625052

Gene position: 2242398-2241211 (Counterclockwise)

Preceding gene: 125625053

Following gene: 125625051

Centisome position: 88.65

GC content: 40.24

Gene sequence:

>1188_bases
ATGGAAAAAACGCTCGCTGTCAATGCAGGCTCCTCATCATTAAAATGGCAACTCTATGAAATGCCAGAAGAAACCGTGAT
TGCTAAGGGGATTTTTGAACGTATTGGTCTATCTGGCTCAATTTCAACAACTAAATTCAACAACGAAGAACACGTTAGAA
AACAAGAAATTGTGGACCATCGTCAAGCTGTTTTATTGCTGATGGATGAGTTAATCCATTTTCAATTAATTCATGAGTTC
CGTGAAATCACAGGAATTGGGCATCGTGTTGTTGCAGGTGGGGAATTTTTTAAAGACTCTACAATTATTACACCTGAGGT
TTTAGCTGAGATAAAAAACCTTTCAACTTTGGCTCCTTTACATAATCCGGCCAACGTTTTAGGGATTGAAGCCTTCCAAC
GTCTCTTACCCGATGCACTTGCGGTCGCTGTTTTCGATACGGCTTTTCATAGCACTTTACCTGAAAAAGCTTATCGTTAT
CCTATTCCAACAAAATATTATGAAGATTATTCAATCAGAAAATATGGGGCACATGGGACTTCACATATGTATGTGGCGCA
AGAAGCAGCAAAAGTCTTAGGAAAACCACTGGAAGAATTGAAGATAATTACGGCCCACATTGGAAATGGAGCTTCAATTA
CAGCGATTGAAGGTGGTAAATCTGTTGATACTTCAATGGGATTCACACCACTTGCTGGTGTAATGATGGGAACACGTGCG
GGGGAAATGGATGCTTCAGTCATTCCTTATATGTTGGAGAGCGATCCGAGTCTTAGAAATGCTCAAGATGTGATTGACAT
TCTCAACAAAGAGTCTGGAGTGCTTGGGGTATCTGAACTTTCTAGTGATATGCGTGACCTTTCAGAAGCAGTAGCCAAAG
GAAATCCTAAAGCGATTCTTGCCTATGAAATGTACGTTGACCGTCTCAAAAAATTTATTGCCCAATATTTTGGCGTATTA
AATGGAGCTGATGCACTTATTTTCACTGCAGGTGTCGGAGAAAATGATACGGCTGTTCGGACGGATGTAGTTAATGGACT
TTCGTGGTTTGGAATGGAAATTGATGAAAGTAAAAATGTTCGTGGAGCTTTTGGCATCATTTCTAAGCCAGAGTCTAAAG
TGAAAGTACTTGTTGTGCCAACAAACGAAGAATTGGTTATTGCTCGTGATGTCGAAGCGGCTAAATAA

Upstream 100 bases:

>100_bases
GATACAAAGTTGTAGGAATCATTGATAGAGGAGAAATGAAATCAACTCTAAAGTATGATATAATTTTAAGAATAAAACGC
ATACATTAAGGAGAGTAAGT

Downstream 100 bases:

>100_bases
TGAAATAAAAATTACTGACCAGGCTGTCAGTAATTTTTTACTTAAGAAAAAGCTGTCAGTAAAATTTATCCAAAAGCTTG
TCATCTATAAAAGAAAGCGC

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase 2 [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MEKTLAVNAGSSSLKWQLYEMPEETVIAKGIFERIGLSGSISTTKFNNEEHVRKQEIVDHRQAVLLLMDELIHFQLIHEF
REITGIGHRVVAGGEFFKDSTIITPEVLAEIKNLSTLAPLHNPANVLGIEAFQRLLPDALAVAVFDTAFHSTLPEKAYRY
PIPTKYYEDYSIRKYGAHGTSHMYVAQEAAKVLGKPLEELKIITAHIGNGASITAIEGGKSVDTSMGFTPLAGVMMGTRA
GEMDASVIPYMLESDPSLRNAQDVIDILNKESGVLGVSELSSDMRDLSEAVAKGNPKAILAYEMYVDRLKKFIAQYFGVL
NGADALIFTAGVGENDTAVRTDVVNGLSWFGMEIDESKNVRGAFGIISKPESKVKVLVVPTNEELVIARDVEAAK

Sequences:

>Translated_395_residues
MEKTLAVNAGSSSLKWQLYEMPEETVIAKGIFERIGLSGSISTTKFNNEEHVRKQEIVDHRQAVLLLMDELIHFQLIHEF
REITGIGHRVVAGGEFFKDSTIITPEVLAEIKNLSTLAPLHNPANVLGIEAFQRLLPDALAVAVFDTAFHSTLPEKAYRY
PIPTKYYEDYSIRKYGAHGTSHMYVAQEAAKVLGKPLEELKIITAHIGNGASITAIEGGKSVDTSMGFTPLAGVMMGTRA
GEMDASVIPYMLESDPSLRNAQDVIDILNKESGVLGVSELSSDMRDLSEAVAKGNPKAILAYEMYVDRLKKFIAQYFGVL
NGADALIFTAGVGENDTAVRTDVVNGLSWFGMEIDESKNVRGAFGIISKPESKVKVLVVPTNEELVIARDVEAAK
>Mature_395_residues
MEKTLAVNAGSSSLKWQLYEMPEETVIAKGIFERIGLSGSISTTKFNNEEHVRKQEIVDHRQAVLLLMDELIHFQLIHEF
REITGIGHRVVAGGEFFKDSTIITPEVLAEIKNLSTLAPLHNPANVLGIEAFQRLLPDALAVAVFDTAFHSTLPEKAYRY
PIPTKYYEDYSIRKYGAHGTSHMYVAQEAAKVLGKPLEELKIITAHIGNGASITAIEGGKSVDTSMGFTPLAGVMMGTRA
GEMDASVIPYMLESDPSLRNAQDVIDILNKESGVLGVSELSSDMRDLSEAVAKGNPKAILAYEMYVDRLKKFIAQYFGVL
NGADALIFTAGVGENDTAVRTDVVNGLSWFGMEIDESKNVRGAFGIISKPESKVKVLVVPTNEELVIARDVEAAK

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=394, Percent_Identity=41.6243654822335, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI145693184, Length=394, Percent_Identity=41.6243654822335, Blast_Score=281, Evalue=7e-77,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 43175; Mature: 43175

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKTLAVNAGSSSLKWQLYEMPEETVIAKGIFERIGLSGSISTTKFNNEEHVRKQEIVDH
CCCEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHH
RQAVLLLMDELIHFQLIHEFREITGIGHRVVAGGEFFKDSTIITPEVLAEIKNLSTLAPL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCC
HNPANVLGIEAFQRLLPDALAVAVFDTAFHSTLPEKAYRYPIPTKYYEDYSIRKYGAHGT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCEEEECCCCC
SHMYVAQEAAKVLGKPLEELKIITAHIGNGASITAIEGGKSVDTSMGFTPLAGVMMGTRA
CCHHHHHHHHHHHCCCHHHHEEEEEECCCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCC
GEMDASVIPYMLESDPSLRNAQDVIDILNKESGVLGVSELSSDMRDLSEAVAKGNPKAIL
CCCCCHHCCHHCCCCCCCCCHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHCCCCCEEE
AYEMYVDRLKKFIAQYFGVLNGADALIFTAGVGENDTAVRTDVVNGLSWFGMEIDESKNV
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHCCHHHCCCEECCCCCC
RGAFGIISKPESKVKVLVVPTNEELVIARDVEAAK
CEEECCCCCCCCCEEEEEEECCCCEEEEECCCCCC
>Mature Secondary Structure
MEKTLAVNAGSSSLKWQLYEMPEETVIAKGIFERIGLSGSISTTKFNNEEHVRKQEIVDH
CCCEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHH
RQAVLLLMDELIHFQLIHEFREITGIGHRVVAGGEFFKDSTIITPEVLAEIKNLSTLAPL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCC
HNPANVLGIEAFQRLLPDALAVAVFDTAFHSTLPEKAYRYPIPTKYYEDYSIRKYGAHGT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCEEEECCCCC
SHMYVAQEAAKVLGKPLEELKIITAHIGNGASITAIEGGKSVDTSMGFTPLAGVMMGTRA
CCHHHHHHHHHHHCCCHHHHEEEEEECCCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCC
GEMDASVIPYMLESDPSLRNAQDVIDILNKESGVLGVSELSSDMRDLSEAVAKGNPKAIL
CCCCCHHCCHHCCCCCCCCCHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHCCCCCEEE
AYEMYVDRLKKFIAQYFGVLNGADALIFTAGVGENDTAVRTDVVNGLSWFGMEIDESKNV
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHCCHHHCCCEECCCCCC
RGAFGIISKPESKVKVLVVPTNEELVIARDVEAAK
CEEECCCCCCCCCEEEEEEECCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]