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The map label for this gene is ackA1 [H]

Identifier: 125625053

GI number: 125625053

Start: 2242557

End: 2243744

Strand: Reverse

Name: ackA1 [H]

Synonym: llmg_2289

Alternate gene names: 125625053

Gene position: 2243744-2242557 (Counterclockwise)

Preceding gene: 125625054

Following gene: 125625052

Centisome position: 88.7

GC content: 38.13

Gene sequence:

>1188_bases
ATGACCAAAACATTAGCAGTAAACGCTGGTTCATCATCTATGAAATGGCAAATGTATGAAATGCCAGAAGAAAAAGTTCT
TGCCAAAGGATTAATTGAACGGATTGGCTTGAAAGATTCAATCGTCACCGTTAAATTTGGAGAAGAAAAAGAAGAACGAG
TTTTTGATATTCCAAATCATACAGAAGCAGTAGAAGTTTTACTTGAAGATTTAAAACGTTTGAAAATAGTAGAGGAATTT
ACTGAAATTACTGGTGTGGGACACCGCGTGGTTGCTGGGGGTGAAATTTTCCAAAAATCAACAGTAGTTACACCAGAAGT
TTTGCAACAAGTTAAAGATTTGTCAGCTCTTGCGCCTTTGCACAATCCAGCAAATGCTGCCGGCATTGAAGCTTTTTTGA
ATTTGTTGCCTGATGCTACTTCAGTAGTTGTTTTTGATACAGCTTTCCATACAACAATGCCTGAAAAAGCTTTCCGTTAT
CCATTGCCTAAAAAATATTATACAGATTATGCCGTACGTAAGTATGGGGCTCATGGAACTTCACATATGTATGTTTCGCA
AGAAGCCGCAAAATTACTTGGTAAACCAATTGAAGAAACAAAAATTATCACGGCGCATATTGGGAATGGCGCATCAATTA
CCGCAGTAAAAGGCGGAGAATCTGTTGATACATCAATGGGCTTCACACCTCTTGCTGGAGTGATGATGGGAACTCGCACA
GGTGAAATGGATCCTTCTGTTTTTCCTTATTTGATTGAAAATGATTCTGAACTCAAAGATGCTCAAGATGTTGTTGATAT
GATGAACAAAGAATCTGGTCTTTATGGGGTATCCGGTATTTCATCAGATATGCGCGATATCATTGCTGCTAAAGATACAG
ATGCTGATGCAAAACTTGCATTTGAGATGTATGTTGACCGCATTCAAAAATTCATCGGTCAATATTTGGCAGTCTTAAAT
GGAGCTGATGCACTTGTCTTTACTGCTGGAATTGGTGAAAATTCTGTTCCTGTTCGAGAAGCAATTCTTTCTGGATTGAC
TTGGTTTGGTATTGAAGTAGACCCTGAAAAAAATGTTTTTGGTGTTGAGGGAGAAATTTCAAAACCAAGCTCACGCCTAA
AAGTTTTTGTAATTCCAACGGATGAAGAACTTGTGATTGCTCGCGACGTTGAGGCACTTAAAAAATAA

Upstream 100 bases:

>100_bases
TAAAAAAATAACTAAGTGAATCATGGCTGAAAAACACAAAAGAAATTGTAATTGTGTTATAATTTAACCGTATTTCAAAT
TCAAGGAAGGTTTATTAAAC

Downstream 100 bases:

>100_bases
TAAATCAAAAGATTTTGTCATCAAACAAGATCTTTTTTTATTTTAAAAATGACAGCAAGATACAAAGTTGTAGGAATCAT
TGATAGAGGAGAAATGAAAT

Product: AckA1 protein

Products: NA

Alternate protein names: Acetokinase 1 [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MTKTLAVNAGSSSMKWQMYEMPEEKVLAKGLIERIGLKDSIVTVKFGEEKEERVFDIPNHTEAVEVLLEDLKRLKIVEEF
TEITGVGHRVVAGGEIFQKSTVVTPEVLQQVKDLSALAPLHNPANAAGIEAFLNLLPDATSVVVFDTAFHTTMPEKAFRY
PLPKKYYTDYAVRKYGAHGTSHMYVSQEAAKLLGKPIEETKIITAHIGNGASITAVKGGESVDTSMGFTPLAGVMMGTRT
GEMDPSVFPYLIENDSELKDAQDVVDMMNKESGLYGVSGISSDMRDIIAAKDTDADAKLAFEMYVDRIQKFIGQYLAVLN
GADALVFTAGIGENSVPVREAILSGLTWFGIEVDPEKNVFGVEGEISKPSSRLKVFVIPTDEELVIARDVEALKK

Sequences:

>Translated_395_residues
MTKTLAVNAGSSSMKWQMYEMPEEKVLAKGLIERIGLKDSIVTVKFGEEKEERVFDIPNHTEAVEVLLEDLKRLKIVEEF
TEITGVGHRVVAGGEIFQKSTVVTPEVLQQVKDLSALAPLHNPANAAGIEAFLNLLPDATSVVVFDTAFHTTMPEKAFRY
PLPKKYYTDYAVRKYGAHGTSHMYVSQEAAKLLGKPIEETKIITAHIGNGASITAVKGGESVDTSMGFTPLAGVMMGTRT
GEMDPSVFPYLIENDSELKDAQDVVDMMNKESGLYGVSGISSDMRDIIAAKDTDADAKLAFEMYVDRIQKFIGQYLAVLN
GADALVFTAGIGENSVPVREAILSGLTWFGIEVDPEKNVFGVEGEISKPSSRLKVFVIPTDEELVIARDVEALKK
>Mature_394_residues
TKTLAVNAGSSSMKWQMYEMPEEKVLAKGLIERIGLKDSIVTVKFGEEKEERVFDIPNHTEAVEVLLEDLKRLKIVEEFT
EITGVGHRVVAGGEIFQKSTVVTPEVLQQVKDLSALAPLHNPANAAGIEAFLNLLPDATSVVVFDTAFHTTMPEKAFRYP
LPKKYYTDYAVRKYGAHGTSHMYVSQEAAKLLGKPIEETKIITAHIGNGASITAVKGGESVDTSMGFTPLAGVMMGTRTG
EMDPSVFPYLIENDSELKDAQDVVDMMNKESGLYGVSGISSDMRDIIAAKDTDADAKLAFEMYVDRIQKFIGQYLAVLNG
ADALVFTAGIGENSVPVREAILSGLTWFGIEVDPEKNVFGVEGEISKPSSRLKVFVIPTDEELVIARDVEALKK

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=396, Percent_Identity=43.6868686868687, Blast_Score=319, Evalue=2e-88,
Organism=Escherichia coli, GI145693184, Length=398, Percent_Identity=40.7035175879397, Blast_Score=288, Evalue=5e-79,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 43145; Mature: 43014

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTLAVNAGSSSMKWQMYEMPEEKVLAKGLIERIGLKDSIVTVKFGEEKEERVFDIPNH
CCCEEEEECCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHEECCCCH
TEAVEVLLEDLKRLKIVEEFTEITGVGHRVVAGGEIFQKSTVVTPEVLQQVKDLSALAPL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHCCCCCCHHHHHHHHHHHHHCCC
HNPANAAGIEAFLNLLPDATSVVVFDTAFHTTMPEKAFRYPLPKKYYTDYAVRKYGAHGT
CCCCCHHHHHHHHHHCCCCCEEEEEECHHHCCCCHHHHCCCCCHHHHHHHHHHHHCCCCC
SHMYVSQEAAKLLGKPIEETKIITAHIGNGASITAVKGGESVDTSMGFTPLAGVMMGTRT
CEEEECHHHHHHHCCCCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCC
GEMDPSVFPYLIENDSELKDAQDVVDMMNKESGLYGVSGISSDMRDIIAAKDTDADAKLA
CCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHCCCCCCHHHHH
FEMYVDRIQKFIGQYLAVLNGADALVFTAGIGENSVPVREAILSGLTWFGIEVDPEKNVF
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCEEEEEEECCCCCEE
GVEGEISKPSSRLKVFVIPTDEELVIARDVEALKK
ECCCCCCCCCCCEEEEEEECCCCEEEEECHHHHCC
>Mature Secondary Structure 
TKTLAVNAGSSSMKWQMYEMPEEKVLAKGLIERIGLKDSIVTVKFGEEKEERVFDIPNH
CCEEEEECCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHEECCCCH
TEAVEVLLEDLKRLKIVEEFTEITGVGHRVVAGGEIFQKSTVVTPEVLQQVKDLSALAPL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHCCCCCCHHHHHHHHHHHHHCCC
HNPANAAGIEAFLNLLPDATSVVVFDTAFHTTMPEKAFRYPLPKKYYTDYAVRKYGAHGT
CCCCCHHHHHHHHHHCCCCCEEEEEECHHHCCCCHHHHCCCCCHHHHHHHHHHHHCCCCC
SHMYVSQEAAKLLGKPIEETKIITAHIGNGASITAVKGGESVDTSMGFTPLAGVMMGTRT
CEEEECHHHHHHHCCCCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCC
GEMDPSVFPYLIENDSELKDAQDVVDMMNKESGLYGVSGISSDMRDIIAAKDTDADAKLA
CCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHCCCCCCHHHHH
FEMYVDRIQKFIGQYLAVLNGADALVFTAGIGENSVPVREAILSGLTWFGIEVDPEKNVF
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCEEEEEEECCCCCEE
GVEGEISKPSSRLKVFVIPTDEELVIARDVEALKK
ECCCCCCCCCCCEEEEEEECCCCEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]