Click here to switch to the map view.

The map label for this gene is purA [H]

Identifier: 125624968

GI number: 125624968

Start: 2167331

End: 2168623

Strand: Reverse

Name: purA [H]

Synonym: llmg_2201

Alternate gene names: 125624968

Gene position: 2168623-2167331 (Counterclockwise)

Preceding gene: 125624969

Following gene: 125624963

Centisome position: 85.73

GC content: 40.37

Gene sequence:

>1293_bases
ATGCCATCAGTAGTAGTTGTCGGAACGCAATGGGGAGACGAAGGAAAAGGTAAAATTACAGATTTCCTAAGCTCAAACGC
AGAAGTTATTGCCCGTTATCAAGGGGGAGACAATGCAGGTCATACCATCGTCATCGACGGCAAAAAGTTCAAACTTCACC
TCATTCCATCAGGGATTTTCTTTCCTGAAAAAATTTCCGTTATCGGAAATGGTGTCGTTGTTAACCCAAAATCTCTGATT
GAAGAAATCGCTTATCTTGCTGAAAATGGAGTTTCTGCAAACAGTTTACGTATTTCTGACCGTGCACACGTTATCTTGCC
ATATCATAAAAAATTAGACTTCCTTCAAGAAGAAGCTAAAGGTGATAAAAAGATTGGGACAACCATTAAAGGGATTGGTC
CAGCCTATATGGATAAAGCGGCTCGTGTCGGAATTCGTATTGCAGACTTACTTGACAAAGAAATCTTCGAGGAACGTTTA
CGTACAAACCTCGAAGTTAAAAACCGTGAATTTGTAAAAATGTATGATTCAGAACCTCTTGAATTTGAAGAAATTTTTGA
AGAATACTATGAGTATGGTCAACAACTTAAAAAATACGTTACAGATACTTCGGTTATCTTGAACGATGCCCTTGATGCAG
GGAAACGTGTCCTTTTTGAAGGGGCACAAGGGGTCATGCTTGATATTGATCAAGGAACTTATCCATTTGTCACTTCATCA
AATCCAGTAGCAGGTGGGGTAACAATCGGTTCTGGTGTTGGTCCATCAAAAATTTCAAAAGTTGTTGGTGTATGTAAAGC
CTATACTTCACGTGTAGGTGACGGTCCATTCCCAACAGAGCTTTTTGATGAAGTGGGACATCAAATTCGTGAAGTTGGAC
ATGAATATGGAACAACAACTGGACGCCCACGTCGTGTTGGTTGGTTTGATTCAGTCGTAATGCGTCACGCAAAACGTGTT
TCAGGTTTAACAAATCTTAGTTTGAACTCAATTGACGTTCTTTCAGGTCTTGAAACAGTAAAAATCTGTGTGGCTTACGA
ACGTAGCAATGGTGAACAAATTACCCACTATCCAGCCTCACTTAAGGAATTGGCAGATTGTAAGCCAATTTATGAAGAAT
TACCGGGTTGGTCAGAAGACATTACTTCATGCCGTACTTTGGAAGAAATACCAGAAGCTGCTCGCAACTATGTTCGTCGT
GTTGGCGAATTAGTTGGTGTACGTATCTCAACTTTCTCTGTAGGACCTGGTCGTGAACAAACTAACGTTTTAGAATCAGT
TTGGGGCGTATAA

Upstream 100 bases:

>100_bases
GAAAATAGATAAAAATGGGAACCTGCCTCAGGCAGAACCACCATCTTTCCTAATTTTCTAGGAAGCTAAGCAGTCGGGCT
CACATCTTAAGGAGAATATT

Downstream 100 bases:

>100_bases
AGTCAAAATAAAAAAGTCAGAGCATGCTCTGACTTTTTTTGTTTACAAGAAGGGTCCAAAGACTTGTGGACTAGAATTTT
TATTATTGACGTTGACATCA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MPSVVVVGTQWGDEGKGKITDFLSSNAEVIARYQGGDNAGHTIVIDGKKFKLHLIPSGIFFPEKISVIGNGVVVNPKSLI
EEIAYLAENGVSANSLRISDRAHVILPYHKKLDFLQEEAKGDKKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
RTNLEVKNREFVKMYDSEPLEFEEIFEEYYEYGQQLKKYVTDTSVILNDALDAGKRVLFEGAQGVMLDIDQGTYPFVTSS
NPVAGGVTIGSGVGPSKISKVVGVCKAYTSRVGDGPFPTELFDEVGHQIREVGHEYGTTTGRPRRVGWFDSVVMRHAKRV
SGLTNLSLNSIDVLSGLETVKICVAYERSNGEQITHYPASLKELADCKPIYEELPGWSEDITSCRTLEEIPEAARNYVRR
VGELVGVRISTFSVGPGREQTNVLESVWGV

Sequences:

>Translated_430_residues
MPSVVVVGTQWGDEGKGKITDFLSSNAEVIARYQGGDNAGHTIVIDGKKFKLHLIPSGIFFPEKISVIGNGVVVNPKSLI
EEIAYLAENGVSANSLRISDRAHVILPYHKKLDFLQEEAKGDKKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
RTNLEVKNREFVKMYDSEPLEFEEIFEEYYEYGQQLKKYVTDTSVILNDALDAGKRVLFEGAQGVMLDIDQGTYPFVTSS
NPVAGGVTIGSGVGPSKISKVVGVCKAYTSRVGDGPFPTELFDEVGHQIREVGHEYGTTTGRPRRVGWFDSVVMRHAKRV
SGLTNLSLNSIDVLSGLETVKICVAYERSNGEQITHYPASLKELADCKPIYEELPGWSEDITSCRTLEEIPEAARNYVRR
VGELVGVRISTFSVGPGREQTNVLESVWGV
>Mature_429_residues
PSVVVVGTQWGDEGKGKITDFLSSNAEVIARYQGGDNAGHTIVIDGKKFKLHLIPSGIFFPEKISVIGNGVVVNPKSLIE
EIAYLAENGVSANSLRISDRAHVILPYHKKLDFLQEEAKGDKKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERLR
TNLEVKNREFVKMYDSEPLEFEEIFEEYYEYGQQLKKYVTDTSVILNDALDAGKRVLFEGAQGVMLDIDQGTYPFVTSSN
PVAGGVTIGSGVGPSKISKVVGVCKAYTSRVGDGPFPTELFDEVGHQIREVGHEYGTTTGRPRRVGWFDSVVMRHAKRVS
GLTNLSLNSIDVLSGLETVKICVAYERSNGEQITHYPASLKELADCKPIYEELPGWSEDITSCRTLEEIPEAARNYVRRV
GELVGVRISTFSVGPGREQTNVLESVWGV

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=422, Percent_Identity=45.0236966824645, Blast_Score=353, Evalue=1e-97,
Organism=Homo sapiens, GI34577063, Length=424, Percent_Identity=41.9811320754717, Blast_Score=334, Evalue=1e-91,
Organism=Homo sapiens, GI40316944, Length=393, Percent_Identity=43.765903307888, Blast_Score=310, Evalue=1e-84,
Organism=Escherichia coli, GI1790620, Length=423, Percent_Identity=48.4633569739953, Blast_Score=421, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI25146553, Length=426, Percent_Identity=42.7230046948357, Blast_Score=331, Evalue=4e-91,
Organism=Caenorhabditis elegans, GI25146555, Length=426, Percent_Identity=42.7230046948357, Blast_Score=331, Evalue=5e-91,
Organism=Saccharomyces cerevisiae, GI6324109, Length=427, Percent_Identity=40.9836065573771, Blast_Score=341, Evalue=1e-94,
Organism=Drosophila melanogaster, GI21356033, Length=418, Percent_Identity=44.2583732057416, Blast_Score=369, Evalue=1e-102,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 47353; Mature: 47222

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSVVVVGTQWGDEGKGKITDFLSSNAEVIARYQGGDNAGHTIVIDGKKFKLHLIPSGIF
CCCEEEEECCCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCEEEEECCEEEEEEECCCCC
FPEKISVIGNGVVVNPKSLIEEIAYLAENGVSANSLRISDRAHVILPYHKKLDFLQEEAK
CCCHHHEECCCEEECHHHHHHHHHHHHHCCCCCCEEEECCCCEEEECCHHHHHHHHHHHC
GDKKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERLRTNLEVKNREFVKMYDSEPL
CCHHHCCHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCC
EFEEIFEEYYEYGQQLKKYVTDTSVILNDALDAGKRVLFEGAQGVMLDIDQGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEECCCCCCEEECC
NPVAGGVTIGSGVGPSKISKVVGVCKAYTSRVGDGPFPTELFDEVGHQIREVGHEYGTTT
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
GRPRRVGWFDSVVMRHAKRVSGLTNLSLNSIDVLSGLETVKICVAYERSNGEQITHYPAS
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCEEEEEEEEECCCCCEEEECCHH
LKELADCKPIYEELPGWSEDITSCRTLEEIPEAARNYVRRVGELVGVRISTFSVGPGREQ
HHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHH
TNVLESVWGV
HHHHHHHHCC
>Mature Secondary Structure 
PSVVVVGTQWGDEGKGKITDFLSSNAEVIARYQGGDNAGHTIVIDGKKFKLHLIPSGIF
CCEEEEECCCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCEEEEECCEEEEEEECCCCC
FPEKISVIGNGVVVNPKSLIEEIAYLAENGVSANSLRISDRAHVILPYHKKLDFLQEEAK
CCCHHHEECCCEEECHHHHHHHHHHHHHCCCCCCEEEECCCCEEEECCHHHHHHHHHHHC
GDKKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERLRTNLEVKNREFVKMYDSEPL
CCHHHCCHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCC
EFEEIFEEYYEYGQQLKKYVTDTSVILNDALDAGKRVLFEGAQGVMLDIDQGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEECCCCCCEEECC
NPVAGGVTIGSGVGPSKISKVVGVCKAYTSRVGDGPFPTELFDEVGHQIREVGHEYGTTT
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
GRPRRVGWFDSVVMRHAKRVSGLTNLSLNSIDVLSGLETVKICVAYERSNGEQITHYPAS
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCEEEEEEEEECCCCCEEEECCHH
LKELADCKPIYEELPGWSEDITSCRTLEEIPEAARNYVRRVGELVGVRISTFSVGPGREQ
HHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHH
TNVLESVWGV
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]