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The map label for this gene is pheS [H]

Identifier: 125624963

GI number: 125624963

Start: 2162127

End: 2163164

Strand: Reverse

Name: pheS [H]

Synonym: llmg_2196

Alternate gene names: 125624963

Gene position: 2163164-2162127 (Counterclockwise)

Preceding gene: 125624968

Following gene: 125624962

Centisome position: 85.52

GC content: 38.63

Gene sequence:

>1038_bases
ATGAACTTACAAGAAAAAATTGAAGACCTTCGCAAGCGGACTTTGTCTGACTTACTCTCTGTTGCAGACGAAAAAACGCT
CAACAATCTTAGAACAGTAATGCTTGGTAAAAAAGGAGAGTTGACTGAAATCCTAAAAGGGATGAAAGATTTAACAAATG
AAGAACGACCAGTTATTGGTGCACTTGCTAATGCTTTTCGTGATGAATTTGGAGCGAAATTTGAAGCGAAAAAACTTGAA
ATTGAACAAGCAGTGATGAACGCGGCTTTGGAGTCTGAAACTTTGGATGTTACTTTACCTGGAAAAGCTCAGAAAAAAGG
AAGTCGTCATATTTTAACTCAAACTCAAGAAGAAATTGAAGAAATTTTCCTTGGAATGGGTTATGAAATTGTTGATGGTT
ATGAAGTCGAAACCGATCATTATAACTTTGAACGAATGAATCTTCCTAAAGATCACCCAGCTCGTGATATGCAAGATACT
TTTTATATTACCAATGAAGTTTTGCTTCGTACGCATACTTCACCAATGCAAGCTCGGACAATGGATGCTCATGATTTTTC
AAAAGGAGGCTTGCGTATGATTGCTCCTGGTCGTGTTTATCGTCGTGATACAGATGATGCGACACACAGTCATCAATTCC
ATCAAATTGAAGGCTTGGTTGTAGATAAAAATATCACAATGGCTGACCTTAAAGGGACACTTGACCTTGTCATGAAAAAA
ATGTTTGGTCAAGAGCGTGAACTACGCTGGCGCCCAAGTTATTTCCCATTTACTGAACCTTCTGTTGAGGTTGATATTTC
TTGTTTCAAATGCGGGGGTAAAGGATGTAATGTCTGCAAACATACCGGTTGGATTGAAATCCTTGGTGCAGGTATGGTCC
ATCCAAACGTACTTGAAATGTCTGGTTTAGATTCTTCTGTTTATTCTGGTTTTGCTTTTGGACTCGGTCAAGAACGTATT
GCCATGTTACGCTATGGAATCAACGATATTCGTGGATTCTATCAAGGCGATGTAAGATTCTTAGAACAATTCGACTGA

Upstream 100 bases:

>100_bases
TTATTAAATAGAAATTGCTCAAATCTCCCTAAAGCTCCATATTTTATATGGACCTTGTCTAATTTTAAATATATATTTTT
TATAAGTTGAAAGGATAAAA

Downstream 100 bases:

>100_bases
CGCTTTAGTCGCATTGTCGATTTCGACATTCGAAAAGGGAGGACTCCCTTCGATGGAGCGATTTGGAAAATAAATAGTAT
AAAAAACTGTGAATAGAAAG

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MNLQEKIEDLRKRTLSDLLSVADEKTLNNLRTVMLGKKGELTEILKGMKDLTNEERPVIGALANAFRDEFGAKFEAKKLE
IEQAVMNAALESETLDVTLPGKAQKKGSRHILTQTQEEIEEIFLGMGYEIVDGYEVETDHYNFERMNLPKDHPARDMQDT
FYITNEVLLRTHTSPMQARTMDAHDFSKGGLRMIAPGRVYRRDTDDATHSHQFHQIEGLVVDKNITMADLKGTLDLVMKK
MFGQERELRWRPSYFPFTEPSVEVDISCFKCGGKGCNVCKHTGWIEILGAGMVHPNVLEMSGLDSSVYSGFAFGLGQERI
AMLRYGINDIRGFYQGDVRFLEQFD

Sequences:

>Translated_345_residues
MNLQEKIEDLRKRTLSDLLSVADEKTLNNLRTVMLGKKGELTEILKGMKDLTNEERPVIGALANAFRDEFGAKFEAKKLE
IEQAVMNAALESETLDVTLPGKAQKKGSRHILTQTQEEIEEIFLGMGYEIVDGYEVETDHYNFERMNLPKDHPARDMQDT
FYITNEVLLRTHTSPMQARTMDAHDFSKGGLRMIAPGRVYRRDTDDATHSHQFHQIEGLVVDKNITMADLKGTLDLVMKK
MFGQERELRWRPSYFPFTEPSVEVDISCFKCGGKGCNVCKHTGWIEILGAGMVHPNVLEMSGLDSSVYSGFAFGLGQERI
AMLRYGINDIRGFYQGDVRFLEQFD
>Mature_345_residues
MNLQEKIEDLRKRTLSDLLSVADEKTLNNLRTVMLGKKGELTEILKGMKDLTNEERPVIGALANAFRDEFGAKFEAKKLE
IEQAVMNAALESETLDVTLPGKAQKKGSRHILTQTQEEIEEIFLGMGYEIVDGYEVETDHYNFERMNLPKDHPARDMQDT
FYITNEVLLRTHTSPMQARTMDAHDFSKGGLRMIAPGRVYRRDTDDATHSHQFHQIEGLVVDKNITMADLKGTLDLVMKK
MFGQERELRWRPSYFPFTEPSVEVDISCFKCGGKGCNVCKHTGWIEILGAGMVHPNVLEMSGLDSSVYSGFAFGLGQERI
AMLRYGINDIRGFYQGDVRFLEQFD

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5729820, Length=293, Percent_Identity=31.740614334471, Blast_Score=128, Evalue=8e-30,
Organism=Homo sapiens, GI4758340, Length=270, Percent_Identity=31.4814814814815, Blast_Score=123, Evalue=3e-28,
Organism=Escherichia coli, GI1788007, Length=324, Percent_Identity=46.9135802469136, Blast_Score=311, Evalue=4e-86,
Organism=Caenorhabditis elegans, GI32563657, Length=277, Percent_Identity=31.0469314079422, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17508957, Length=277, Percent_Identity=31.0469314079422, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI32566635, Length=251, Percent_Identity=35.0597609561753, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6321087, Length=366, Percent_Identity=28.9617486338798, Blast_Score=138, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6325304, Length=296, Percent_Identity=28.7162162162162, Blast_Score=107, Evalue=3e-24,
Organism=Drosophila melanogaster, GI18858079, Length=277, Percent_Identity=31.4079422382672, Blast_Score=134, Evalue=6e-32,
Organism=Drosophila melanogaster, GI17137424, Length=293, Percent_Identity=30.3754266211604, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 39231; Mature: 39231

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLQEKIEDLRKRTLSDLLSVADEKTLNNLRTVMLGKKGELTEILKGMKDLTNEERPVIG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHH
ALANAFRDEFGAKFEAKKLEIEQAVMNAALESETLDVTLPGKAQKKGSRHILTQTQEEIE
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHCCCCCCHHHHHHHHHH
EIFLGMGYEIVDGYEVETDHYNFERMNLPKDHPARDMQDTFYITNEVLLRTHTSPMQART
HHHHHCCCEEECCEEEECCCCCCCCCCCCCCCCCCCCCHHEEEECCEEEEECCCCCHHHC
MDAHDFSKGGLRMIAPGRVYRRDTDDATHSHQFHQIEGLVVDKNITMADLKGTLDLVMKK
CCCCCCCCCCEEEECCCCEEECCCCCCHHCCHHHEECCEEEECCCCHHHHHHHHHHHHHH
MFGQERELRWRPSYFPFTEPSVEVDISCFKCGGKGCNVCKHTGWIEILGAGMVHPNVLEM
HHCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHCCCHHEEEECCCCCCCEEEE
SGLDSSVYSGFAFGLGQERIAMLRYGINDIRGFYQGDVRFLEQFD
CCCCHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLQEKIEDLRKRTLSDLLSVADEKTLNNLRTVMLGKKGELTEILKGMKDLTNEERPVIG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHH
ALANAFRDEFGAKFEAKKLEIEQAVMNAALESETLDVTLPGKAQKKGSRHILTQTQEEIE
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHCCCCCCHHHHHHHHHH
EIFLGMGYEIVDGYEVETDHYNFERMNLPKDHPARDMQDTFYITNEVLLRTHTSPMQART
HHHHHCCCEEECCEEEECCCCCCCCCCCCCCCCCCCCCHHEEEECCEEEEECCCCCHHHC
MDAHDFSKGGLRMIAPGRVYRRDTDDATHSHQFHQIEGLVVDKNITMADLKGTLDLVMKK
CCCCCCCCCCEEEECCCCEEECCCCCCHHCCHHHEECCEEEECCCCHHHHHHHHHHHHHH
MFGQERELRWRPSYFPFTEPSVEVDISCFKCGGKGCNVCKHTGWIEILGAGMVHPNVLEM
HHCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHCCCHHEEEECCCCCCCEEEE
SGLDSSVYSGFAFGLGQERIAMLRYGINDIRGFYQGDVRFLEQFD
CCCCHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]