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The map label for this gene is menE [H]

Identifier: 125624621

GI number: 125624621

Start: 1812710

End: 1814065

Strand: Direct

Name: menE [H]

Synonym: llmg_1832

Alternate gene names: 125624621

Gene position: 1812710-1814065 (Clockwise)

Preceding gene: 125624620

Following gene: 125624622

Centisome position: 71.66

GC content: 37.39

Gene sequence:

>1356_bases
ATGAAATGGTTAAAAAAACAGGCGGAACTTTATCCCCAAAAAACATTTTTAAATGACTTTACTTTTGAACAAATCAATCA
ACGAGTGGATAAGATAGCAAGACATTTAGCTCCACTCGTCGCTAAAAAGACTAGAGTTGCGCTCCTTTCAGAGAATTCGG
TTGAAATGGCGGTCCTGTTGTTTGCCTTACTCGGTTTGTCAAAAGAGATTCTTCTTTTGAATACACATCTAACCACATAC
GAACTGACTGAGCAAGTCAATGAACTCGAAATTGATAGAGTCTTCAGCTCTGATTTATTGAAAGAAAAAATGACTGACAG
CATTTCTTTTTCAGATATTTTGGCAACAACTGCTGAACCTGTAAGTCTGTCAGTAAATTTTCCTGACGAAAAAATCGCTG
TTATCATGAATACTTCGGCGACTACCGGAAAATTTAAATCCGTGCCCATCACATGGGGCATGATTTCAAACCATGTTAAA
GCCTCGCAAAAGACGCTCGGTCTTTACGAAGATGATAATTGGCTCATCATCTTACCTATGTTTCACGTCAGCGGATTATC
AATCATCATGCGTAGTCTATACAACGCAACATCAGCCACAATTTTTGATAAATTTGATGAAAATCAGCTTCTAGAAGTGG
TCAATTCTGGAAAAATAAACATGATTTCACTCGTTCCGACCATTCTGACCAGAATTGCCAACAATTTGAATGGAAATAAT
TTACGATTAATTTTACTTGGTGGTGAATTTATTCCTCAACCTTTAATCCAAAAATGCCAAGAATTAGCTTTGCCAGTCTA
TAAAACATATGGAATGACCGAAAGTTTTTCCCAATCTGTTACTTTTAATATTTTAGATTTTCCAGATAAAACAAGCTCTG
TTGGTCGACCTTTACCAGGAGTTAAAATTGAAATTCGCAATCCTGATCTTTCTGGCGTTGGCGAAATTTGGCTAAAATCT
CCCATGCTGATGACCGCTTATTTAGGGCAAAAGCCTTATGGTGCTGCTTTTGAAACAGGCGATATTGGCTATCTTGATAG
GGATGGCTTTCTCTATCTGCTCAACCGCAGAAAAGATATCATCATTTCTGGTGGCGAAAATATTTATCCCAAAGAAATCG
AAGATTTAGTATATTCTCTTCCTGAAATTAAGGAATGTGCCGTCGTTGCAAAAACTGATGCCAAATGGGGTCAAGTGCCG
ATTCTCTTTGTTTCAGGCAATATTTCCCAAGAAAAATTAGAAAACTTTCTCACTGAAAAGCTTGCAAAATATAAGCGTCC
GCAGACCATCATTTTCATGGATGAATTGCCCAAAAACGCTTCTGGAAAAATCTTAAGAAAAGAGCTCAAAGCATGA

Upstream 100 bases:

>100_bases
ACTGACAGCTTTTTAAAATTATGAAAAATTACTGACAAGATTTCTGTCAGTAAAAAAATACAAATGAAGGGAAAAATTGA
GTTCACTCACATCTTAAATA

Downstream 100 bases:

>100_bases
AAATTGAAAAAATCACAATGTTTCATGTTCAACTCCCCATGAAATTCAATTTTAAGACCGCCAAAGGTTCCTTAAATCTC
CGTGATACAATTATTATAAA

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MKWLKKQAELYPQKTFLNDFTFEQINQRVDKIARHLAPLVAKKTRVALLSENSVEMAVLLFALLGLSKEILLLNTHLTTY
ELTEQVNELEIDRVFSSDLLKEKMTDSISFSDILATTAEPVSLSVNFPDEKIAVIMNTSATTGKFKSVPITWGMISNHVK
ASQKTLGLYEDDNWLIILPMFHVSGLSIIMRSLYNATSATIFDKFDENQLLEVVNSGKINMISLVPTILTRIANNLNGNN
LRLILLGGEFIPQPLIQKCQELALPVYKTYGMTESFSQSVTFNILDFPDKTSSVGRPLPGVKIEIRNPDLSGVGEIWLKS
PMLMTAYLGQKPYGAAFETGDIGYLDRDGFLYLLNRRKDIIISGGENIYPKEIEDLVYSLPEIKECAVVAKTDAKWGQVP
ILFVSGNISQEKLENFLTEKLAKYKRPQTIIFMDELPKNASGKILRKELKA

Sequences:

>Translated_451_residues
MKWLKKQAELYPQKTFLNDFTFEQINQRVDKIARHLAPLVAKKTRVALLSENSVEMAVLLFALLGLSKEILLLNTHLTTY
ELTEQVNELEIDRVFSSDLLKEKMTDSISFSDILATTAEPVSLSVNFPDEKIAVIMNTSATTGKFKSVPITWGMISNHVK
ASQKTLGLYEDDNWLIILPMFHVSGLSIIMRSLYNATSATIFDKFDENQLLEVVNSGKINMISLVPTILTRIANNLNGNN
LRLILLGGEFIPQPLIQKCQELALPVYKTYGMTESFSQSVTFNILDFPDKTSSVGRPLPGVKIEIRNPDLSGVGEIWLKS
PMLMTAYLGQKPYGAAFETGDIGYLDRDGFLYLLNRRKDIIISGGENIYPKEIEDLVYSLPEIKECAVVAKTDAKWGQVP
ILFVSGNISQEKLENFLTEKLAKYKRPQTIIFMDELPKNASGKILRKELKA
>Mature_451_residues
MKWLKKQAELYPQKTFLNDFTFEQINQRVDKIARHLAPLVAKKTRVALLSENSVEMAVLLFALLGLSKEILLLNTHLTTY
ELTEQVNELEIDRVFSSDLLKEKMTDSISFSDILATTAEPVSLSVNFPDEKIAVIMNTSATTGKFKSVPITWGMISNHVK
ASQKTLGLYEDDNWLIILPMFHVSGLSIIMRSLYNATSATIFDKFDENQLLEVVNSGKINMISLVPTILTRIANNLNGNN
LRLILLGGEFIPQPLIQKCQELALPVYKTYGMTESFSQSVTFNILDFPDKTSSVGRPLPGVKIEIRNPDLSGVGEIWLKS
PMLMTAYLGQKPYGAAFETGDIGYLDRDGFLYLLNRRKDIIISGGENIYPKEIEDLVYSLPEIKECAVVAKTDAKWGQVP
ILFVSGNISQEKLENFLTEKLAKYKRPQTIIFMDELPKNASGKILRKELKA

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=399, Percent_Identity=27.0676691729323, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI187761345, Length=387, Percent_Identity=26.3565891472868, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI187761343, Length=387, Percent_Identity=26.3565891472868, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI42544132, Length=279, Percent_Identity=28.3154121863799, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI38505220, Length=353, Percent_Identity=24.929178470255, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI115511026, Length=392, Percent_Identity=25.2551020408163, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI122937307, Length=237, Percent_Identity=29.957805907173, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI13375727, Length=363, Percent_Identity=22.8650137741047, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI157311624, Length=394, Percent_Identity=24.6192893401015, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI157311622, Length=394, Percent_Identity=24.6192893401015, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI27477105, Length=124, Percent_Identity=33.0645161290323, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788595, Length=325, Percent_Identity=30.4615384615385, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1788107, Length=514, Percent_Identity=26.0700389105058, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI145693145, Length=376, Percent_Identity=27.6595744680851, Blast_Score=125, Evalue=8e-30,
Organism=Escherichia coli, GI221142682, Length=507, Percent_Identity=24.0631163708087, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1789201, Length=464, Percent_Identity=24.1379310344828, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1786810, Length=485, Percent_Identity=24.3298969072165, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786529, Length=551, Percent_Identity=23.049001814882, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17558820, Length=341, Percent_Identity=33.7243401759531, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17559526, Length=494, Percent_Identity=26.7206477732794, Blast_Score=148, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI32563687, Length=445, Percent_Identity=28.5393258426966, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17531443, Length=493, Percent_Identity=24.9492900608519, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17557194, Length=379, Percent_Identity=27.1767810026385, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17538037, Length=381, Percent_Identity=24.6719160104987, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71985884, Length=348, Percent_Identity=27.0114942528736, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI25141359, Length=474, Percent_Identity=23.8396624472574, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71996755, Length=297, Percent_Identity=29.6296296296296, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17560140, Length=489, Percent_Identity=24.5398773006135, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17560308, Length=355, Percent_Identity=24.7887323943662, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71994690, Length=357, Percent_Identity=24.9299719887955, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71994694, Length=357, Percent_Identity=24.9299719887955, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71994703, Length=357, Percent_Identity=24.9299719887955, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17556356, Length=333, Percent_Identity=26.1261261261261, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=349, Percent_Identity=30.0859598853868, Blast_Score=129, Evalue=9e-31,
Organism=Saccharomyces cerevisiae, GI6319264, Length=368, Percent_Identity=23.0978260869565, Blast_Score=77, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6323182, Length=390, Percent_Identity=22.8205128205128, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI21355181, Length=466, Percent_Identity=27.2532188841202, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI18859661, Length=344, Percent_Identity=27.3255813953488, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI20130357, Length=357, Percent_Identity=27.4509803921569, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24653035, Length=465, Percent_Identity=23.6559139784946, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI21356947, Length=372, Percent_Identity=25.2688172043011, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI161076582, Length=469, Percent_Identity=23.0277185501066, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24581924, Length=357, Percent_Identity=26.0504201680672, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24648255, Length=468, Percent_Identity=23.0769230769231, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24648260, Length=355, Percent_Identity=23.6619718309859, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24648253, Length=390, Percent_Identity=24.1025641025641, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356441, Length=399, Percent_Identity=26.5664160401003, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24648257, Length=512, Percent_Identity=22.0703125, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281365686, Length=364, Percent_Identity=24.7252747252747, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19921316, Length=162, Percent_Identity=31.4814814814815, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21355225, Length=407, Percent_Identity=26.044226044226, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24656500, Length=360, Percent_Identity=23.0555555555556, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI19922652, Length=358, Percent_Identity=22.3463687150838, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI21358303, Length=322, Percent_Identity=25.4658385093168, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 50696; Mature: 50696

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKWLKKQAELYPQKTFLNDFTFEQINQRVDKIARHLAPLVAKKTRVALLSENSVEMAVLL
CCHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHH
FALLGLSKEILLLNTHLTTYELTEQVNELEIDRVFSSDLLKEKMTDSISFSDILATTAEP
HHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCC
VSLSVNFPDEKIAVIMNTSATTGKFKSVPITWGMISNHVKASQKTLGLYEDDNWLIILPM
EEEEEECCCCEEEEEEECCCCCCCCEECCEEHHHHHHHHHHHHHHCCEEECCCEEEEEEC
FHVSGLSIIMRSLYNATSATIFDKFDENQLLEVVNSGKINMISLVPTILTRIANNLNGNN
HHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCC
LRLILLGGEFIPQPLIQKCQELALPVYKTYGMTESFSQSVTFNILDFPDKTSSVGRPLPG
EEEEEECCCCCCHHHHHHHHHHCCCHHHHCCCCCHHCCCEEEEEEECCCCCCCCCCCCCC
VKIEIRNPDLSGVGEIWLKSPMLMTAYLGQKPYGAAFETGDIGYLDRDGFLYLLNRRKDI
CEEEEECCCCCCCHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCEECCCCEEEEEECCCEE
IISGGENIYPKEIEDLVYSLPEIKECAVVAKTDAKWGQVPILFVSGNISQEKLENFLTEK
EEECCCCCCHHHHHHHHHCCCCHHHCEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHH
LAKYKRPQTIIFMDELPKNASGKILRKELKA
HHHHCCCCEEEEEECCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MKWLKKQAELYPQKTFLNDFTFEQINQRVDKIARHLAPLVAKKTRVALLSENSVEMAVLL
CCHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHH
FALLGLSKEILLLNTHLTTYELTEQVNELEIDRVFSSDLLKEKMTDSISFSDILATTAEP
HHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCC
VSLSVNFPDEKIAVIMNTSATTGKFKSVPITWGMISNHVKASQKTLGLYEDDNWLIILPM
EEEEEECCCCEEEEEEECCCCCCCCEECCEEHHHHHHHHHHHHHHCCEEECCCEEEEEEC
FHVSGLSIIMRSLYNATSATIFDKFDENQLLEVVNSGKINMISLVPTILTRIANNLNGNN
HHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCC
LRLILLGGEFIPQPLIQKCQELALPVYKTYGMTESFSQSVTFNILDFPDKTSSVGRPLPG
EEEEEECCCCCCHHHHHHHHHHCCCHHHHCCCCCHHCCCEEEEEEECCCCCCCCCCCCCC
VKIEIRNPDLSGVGEIWLKSPMLMTAYLGQKPYGAAFETGDIGYLDRDGFLYLLNRRKDI
CEEEEECCCCCCCHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCEECCCCEEEEEECCCEE
IISGGENIYPKEIEDLVYSLPEIKECAVVAKTDAKWGQVPILFVSGNISQEKLENFLTEK
EEECCCCCCHHHHHHHHHCCCCHHHCEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHH
LAKYKRPQTIIFMDELPKNASGKILRKELKA
HHHHCCCCEEEEEECCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]