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The map label for this gene is thrA [H]

Identifier: 125624609

GI number: 125624609

Start: 1800198

End: 1801550

Strand: Direct

Name: thrA [H]

Synonym: llmg_1820

Alternate gene names: 125624609

Gene position: 1800198-1801550 (Clockwise)

Preceding gene: 125624608

Following gene: 125624612

Centisome position: 71.17

GC content: 38.36

Gene sequence:

>1353_bases
GTGAAAGTTGCAAAATTTGGGGGTTCATCACTCGCATCAGCTACTCAGATCAAAAGAGTTTTTGATATTGTCCAAGCGGA
TAAATTAAGAAAATTTATTGTTGTTTCTGCACCAGGAAAACGTTCGGGGGCTGATACTAAAGTTACCGATGCTTTAATTT
CTTATTATAAAGCTTACAAAACAGCCGATGATAAAGGAATTCAATATTATCAAAATTGGATTATTGCAAGATTTCGTGAA
ATCTATGATGAACTTGAGCTAAAAAGTTCTGTCATTGATGAAATTGCAGACAATATCCACAACTTAGCTCATTTAGATTT
GGAGAACGTCTTTACTTATGACACTTTTTTAGCTGCTGGAGAAAATAATAACGCTAAATTAATTGCTGACTTCTTTACAC
ATTCTGGCTTACCGAGTCGATACGTTCATCCATCAGATGCTGGAATCACTGTTTCTTCTGACCCAGGAAATGCTCGCTTA
CTTGTAGGGGCTTATGAACATATCAAGTATCTTAATGACCTTGAAGAAACCCTAATTATTCCCGGATTTTTTGGTGTTAC
CAAGGAAGGCGAAGTCTGTACTTTTTCTCGTGGGGGTTCTGACATTACTGGCTCAATTATTGCAGCTGGTGTGAGCGCTG
AACTTTATGAAAATTTCACTGATGTCAATGGAATTTTTGCCGCGCATCCCGGAATCGTAAAAAATCCAGCTTATATCGCT
GAATTAACTTATCGTGAAATGCGGGAGTTGGCTTATTCTGGTTTTTCTGTCTTGCATGATGAAGCCCTTGTCCCTGCTTA
TCGTGCCCGTGTCCCACTGGTTATTAAAAATACGAATAATCCTGACCATCCAGGTACAAAAATTGTCGTGCAAAGGGAAA
CTAAAGGTTCTCCCGTAGTCGGAATTGCTTCATCTAGCGCCTTCTCTTCTCTTTATGTCAGTAAATATCTGATGAATCGC
GAAGTAGGATTTGGACGAAAGGTGCTTTCCGTTTTGGAAAATTTAGGTGTTCGTTTTGAACATATGCCCACAGGAATTGA
TGACATGTCTATCATTATTAGAAGTCGTTATTTGACTCAACCTATTTCCAAAGCCTTAATTTCAAATCTCACCGAGTCCC
TTCATCCTGATGAAGTTTATATTGACCGTGATTTCTCAATTATTACCATTGTAGGTGAAGACATGAAAGAACATGTCGGT
GTCACAGCTAGGGCAACTGCTGCACTTTCTAAGCAAGGTATCTCTATTGCAATGCTTTCCCAAGGTTCAAGTGAAGTTTC
TGTGATGTTTATTGTCAAAGAAAAATATGAGCAAGAGGCATTGAAAGCCATTTATTCAGCCTTTTTTGATTAA

Upstream 100 bases:

>100_bases
CTCCAAAATAAAATGAGTTATTTATTGAAAATTATCTGAAAATTAGCTATAATAAATCAATTTATAATTTAATTTAATCT
TGAAGGGAAGGTAATTTTAA

Downstream 100 bases:

>100_bases
TTATTTAAATAAAAAATAGCTCTAAGAGCTATTTTTTTTATTCGTTTATTTTTTCCACAGTTTCCAATTTCTCACTGGAA
GTTACACGATTAGAAAAATT

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MKVAKFGGSSLASATQIKRVFDIVQADKLRKFIVVSAPGKRSGADTKVTDALISYYKAYKTADDKGIQYYQNWIIARFRE
IYDELELKSSVIDEIADNIHNLAHLDLENVFTYDTFLAAGENNNAKLIADFFTHSGLPSRYVHPSDAGITVSSDPGNARL
LVGAYEHIKYLNDLEETLIIPGFFGVTKEGEVCTFSRGGSDITGSIIAAGVSAELYENFTDVNGIFAAHPGIVKNPAYIA
ELTYREMRELAYSGFSVLHDEALVPAYRARVPLVIKNTNNPDHPGTKIVVQRETKGSPVVGIASSSAFSSLYVSKYLMNR
EVGFGRKVLSVLENLGVRFEHMPTGIDDMSIIIRSRYLTQPISKALISNLTESLHPDEVYIDRDFSIITIVGEDMKEHVG
VTARATAALSKQGISIAMLSQGSSEVSVMFIVKEKYEQEALKAIYSAFFD

Sequences:

>Translated_450_residues
MKVAKFGGSSLASATQIKRVFDIVQADKLRKFIVVSAPGKRSGADTKVTDALISYYKAYKTADDKGIQYYQNWIIARFRE
IYDELELKSSVIDEIADNIHNLAHLDLENVFTYDTFLAAGENNNAKLIADFFTHSGLPSRYVHPSDAGITVSSDPGNARL
LVGAYEHIKYLNDLEETLIIPGFFGVTKEGEVCTFSRGGSDITGSIIAAGVSAELYENFTDVNGIFAAHPGIVKNPAYIA
ELTYREMRELAYSGFSVLHDEALVPAYRARVPLVIKNTNNPDHPGTKIVVQRETKGSPVVGIASSSAFSSLYVSKYLMNR
EVGFGRKVLSVLENLGVRFEHMPTGIDDMSIIIRSRYLTQPISKALISNLTESLHPDEVYIDRDFSIITIVGEDMKEHVG
VTARATAALSKQGISIAMLSQGSSEVSVMFIVKEKYEQEALKAIYSAFFD
>Mature_450_residues
MKVAKFGGSSLASATQIKRVFDIVQADKLRKFIVVSAPGKRSGADTKVTDALISYYKAYKTADDKGIQYYQNWIIARFRE
IYDELELKSSVIDEIADNIHNLAHLDLENVFTYDTFLAAGENNNAKLIADFFTHSGLPSRYVHPSDAGITVSSDPGNARL
LVGAYEHIKYLNDLEETLIIPGFFGVTKEGEVCTFSRGGSDITGSIIAAGVSAELYENFTDVNGIFAAHPGIVKNPAYIA
ELTYREMRELAYSGFSVLHDEALVPAYRARVPLVIKNTNNPDHPGTKIVVQRETKGSPVVGIASSSAFSSLYVSKYLMNR
EVGFGRKVLSVLENLGVRFEHMPTGIDDMSIIIRSRYLTQPISKALISNLTESLHPDEVYIDRDFSIITIVGEDMKEHVG
VTARATAALSKQGISIAMLSQGSSEVSVMFIVKEKYEQEALKAIYSAFFD

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=480, Percent_Identity=26.4583333333333, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1790455, Length=466, Percent_Identity=23.175965665236, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1790376, Length=473, Percent_Identity=22.8329809725159, Blast_Score=73, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6320893, Length=374, Percent_Identity=24.331550802139, Blast_Score=104, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 49561; Mature: 49561

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAKFGGSSLASATQIKRVFDIVQADKLRKFIVVSAPGKRSGADTKVTDALISYYKAYK
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHC
TADDKGIQYYQNWIIARFREIYDELELKSSVIDEIADNIHNLAHLDLENVFTYDTFLAAG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEEC
ENNNAKLIADFFTHSGLPSRYVHPSDAGITVSSDPGNARLLVGAYEHIKYLNDLEETLII
CCCCCEEEEEEHHCCCCCCCCCCCCCCCEEEECCCCCEEEEEEHHHHHHHHHHHHHHEEC
PGFFGVTKEGEVCTFSRGGSDITGSIIAAGVSAELYENFTDVNGIFAAHPGIVKNPAYIA
CCCCCCCCCCCEEEECCCCCCCCCHHHEECCHHHHHHHHHCCCCEEECCCCCCCCCHHHH
ELTYREMRELAYSGFSVLHDEALVPAYRARVPLVIKNTNNPDHPGTKIVVQRETKGSPVV
HHHHHHHHHHHHCCHHHHHHHHHCCHHHCCCCEEEECCCCCCCCCCEEEEEECCCCCCEE
GIASSSAFSSLYVSKYLMNREVGFGRKVLSVLENLGVRFEHMPTGIDDMSIIIRSRYLTQ
EEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHH
PISKALISNLTESLHPDEVYIDRDFSIITIVGEDMKEHVGVTARATAALSKQGISIAMLS
HHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCHHHHCCCCHHHHHHHHHCCCEEEEEC
QGSSEVSVMFIVKEKYEQEALKAIYSAFFD
CCCCCEEEEEEEHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVAKFGGSSLASATQIKRVFDIVQADKLRKFIVVSAPGKRSGADTKVTDALISYYKAYK
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHC
TADDKGIQYYQNWIIARFREIYDELELKSSVIDEIADNIHNLAHLDLENVFTYDTFLAAG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEEC
ENNNAKLIADFFTHSGLPSRYVHPSDAGITVSSDPGNARLLVGAYEHIKYLNDLEETLII
CCCCCEEEEEEHHCCCCCCCCCCCCCCCEEEECCCCCEEEEEEHHHHHHHHHHHHHHEEC
PGFFGVTKEGEVCTFSRGGSDITGSIIAAGVSAELYENFTDVNGIFAAHPGIVKNPAYIA
CCCCCCCCCCCEEEECCCCCCCCCHHHEECCHHHHHHHHHCCCCEEECCCCCCCCCHHHH
ELTYREMRELAYSGFSVLHDEALVPAYRARVPLVIKNTNNPDHPGTKIVVQRETKGSPVV
HHHHHHHHHHHHCCHHHHHHHHHCCHHHCCCCEEEECCCCCCCCCCEEEEEECCCCCCEE
GIASSSAFSSLYVSKYLMNREVGFGRKVLSVLENLGVRFEHMPTGIDDMSIIIRSRYLTQ
EEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHH
PISKALISNLTESLHPDEVYIDRDFSIITIVGEDMKEHVGVTARATAALSKQGISIAMLS
HHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCHHHHCCCCHHHHHHHHHCCCEEEEEC
QGSSEVSVMFIVKEKYEQEALKAIYSAFFD
CCCCCEEEEEEEHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]