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The map label for this gene is prsA [H]

Identifier: 125624533

GI number: 125624533

Start: 1724489

End: 1725463

Strand: Reverse

Name: prsA [H]

Synonym: llmg_1743

Alternate gene names: 125624533

Gene position: 1725463-1724489 (Counterclockwise)

Preceding gene: 125624534

Following gene: 125624532

Centisome position: 68.21

GC content: 41.13

Gene sequence:

>975_bases
ATGTCGTATTCAAATTTGAAGTTGTTTTCGCTCTCTTCTAACCACGAATTAGCCCAAAAAGTGGCTAAAGAAATTGGAAT
TGAGCTTGGAAAAGTAAGTGTTGGTGCACATTCGGATGGCGAAACTGTCGTTCATATTGACGAATCAGTTCGTGGTGATC
ATGTGTTTATCCTTCAATCAACAAGTGACCCTGTCAATGATAACTTGATGGAACTCTTGATTATGATGGATGCATTGCGT
CGTGCCTCTGCTGCATCAATTAACATTGTCTTACCTTACTATGGTTATGCTCGTCAAGACCGTAAAGCTCGTGCTCGTGA
ACCAATCACATCAAAACTCGTAGCAAACATGCTTCAAATTGCTGGTGCCGACCGTTTAATTACTTTCGACCTTCACGCAC
CACAAATTCAAGGATTCTTCAACATTCCAGTTGACCACCTTATGGGTTCACCATTGATTGCTGAATATTTCCGTCGTCAA
TTGGTTTCTGCTGGTGATGATATCGTTGTCGTTTCGCCTGACCATGGTGGAGTAGGACGTGCGCGCAAGTTAGCAAACTT
CTTGAAAGCACCACTTGCTATCATTGATAAACGTCGTCCTCGTGCCAATGTGGCAGAAATTATGAATATCATCGGTGATG
TTCAAGGGAAAAAATGTATCTTGATTGATGACATGATTGATACGGCCGGAACAATTACTTTAGCGGCCAATGCCCTTAAA
GAACTTGGTGCAACAGAAGTCTACGCTTCATGTACTCATGCCGTACTTTCTGGACCAGCTATCGAGAGAATTAATAATTC
AGCAATTACTAAACTCGTTGTTTTGGATACTATTGAAATGCCAGAAGAACGTCAAAGTGAAAAAATTGTTCAACTTTCAA
TTGCTCACTTGCTTGCTGATGCGATTATTCGTATTCACGAACGCCGTCCATTGTCACCACTCTTTGAATTACATCTTCCT
TCAGAACAAATCTAA

Upstream 100 bases:

>100_bases
TAAAGCCGATGAGCGTTTGAAAAAATTGAGAATTTAATGTAAAATAGAATTACATAAAATAGAACTGCTATGTTCTGTAG
GAATTAAAGAAAGGCTTTTC

Downstream 100 bases:

>100_bases
ACTAGTAATTGTTGGAATTCAAAGTGGATTTAGGATAAGACTTCAAGCGACCAGAGGTCGCTTTTTTAAAAAATGGAACT
AGAAAAAATTAGAAAACTTT

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK 1; Phosphoribosyl pyrophosphate synthase 1; P-Rib-PP synthase 1; PRPP synthase 1 [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MSYSNLKLFSLSSNHELAQKVAKEIGIELGKVSVGAHSDGETVVHIDESVRGDHVFILQSTSDPVNDNLMELLIMMDALR
RASAASINIVLPYYGYARQDRKARAREPITSKLVANMLQIAGADRLITFDLHAPQIQGFFNIPVDHLMGSPLIAEYFRRQ
LVSAGDDIVVVSPDHGGVGRARKLANFLKAPLAIIDKRRPRANVAEIMNIIGDVQGKKCILIDDMIDTAGTITLAANALK
ELGATEVYASCTHAVLSGPAIERINNSAITKLVVLDTIEMPEERQSEKIVQLSIAHLLADAIIRIHERRPLSPLFELHLP
SEQI

Sequences:

>Translated_324_residues
MSYSNLKLFSLSSNHELAQKVAKEIGIELGKVSVGAHSDGETVVHIDESVRGDHVFILQSTSDPVNDNLMELLIMMDALR
RASAASINIVLPYYGYARQDRKARAREPITSKLVANMLQIAGADRLITFDLHAPQIQGFFNIPVDHLMGSPLIAEYFRRQ
LVSAGDDIVVVSPDHGGVGRARKLANFLKAPLAIIDKRRPRANVAEIMNIIGDVQGKKCILIDDMIDTAGTITLAANALK
ELGATEVYASCTHAVLSGPAIERINNSAITKLVVLDTIEMPEERQSEKIVQLSIAHLLADAIIRIHERRPLSPLFELHLP
SEQI
>Mature_323_residues
SYSNLKLFSLSSNHELAQKVAKEIGIELGKVSVGAHSDGETVVHIDESVRGDHVFILQSTSDPVNDNLMELLIMMDALRR
ASAASINIVLPYYGYARQDRKARAREPITSKLVANMLQIAGADRLITFDLHAPQIQGFFNIPVDHLMGSPLIAEYFRRQL
VSAGDDIVVVSPDHGGVGRARKLANFLKAPLAIIDKRRPRANVAEIMNIIGDVQGKKCILIDDMIDTAGTITLAANALKE
LGATEVYASCTHAVLSGPAIERINNSAITKLVVLDTIEMPEERQSEKIVQLSIAHLLADAIIRIHERRPLSPLFELHLPS
EQI

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506127, Length=319, Percent_Identity=48.58934169279, Blast_Score=296, Evalue=3e-80,
Organism=Homo sapiens, GI4506129, Length=319, Percent_Identity=48.2758620689655, Blast_Score=293, Evalue=1e-79,
Organism=Homo sapiens, GI28557709, Length=317, Percent_Identity=47.9495268138801, Blast_Score=293, Evalue=1e-79,
Organism=Homo sapiens, GI84875539, Length=322, Percent_Identity=47.8260869565217, Blast_Score=288, Evalue=5e-78,
Organism=Homo sapiens, GI4506133, Length=350, Percent_Identity=37.7142857142857, Blast_Score=189, Evalue=2e-48,
Organism=Homo sapiens, GI194018537, Length=347, Percent_Identity=36.0230547550432, Blast_Score=174, Evalue=8e-44,
Organism=Homo sapiens, GI310128524, Length=150, Percent_Identity=35.3333333333333, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI310115209, Length=150, Percent_Identity=35.3333333333333, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI310118259, Length=150, Percent_Identity=35.3333333333333, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI310119946, Length=150, Percent_Identity=35.3333333333333, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1787458, Length=315, Percent_Identity=52.0634920634921, Blast_Score=318, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI25149168, Length=317, Percent_Identity=46.6876971608833, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI17554702, Length=317, Percent_Identity=46.6876971608833, Blast_Score=284, Evalue=4e-77,
Organism=Caenorhabditis elegans, GI71989924, Length=317, Percent_Identity=46.6876971608833, Blast_Score=283, Evalue=9e-77,
Organism=Caenorhabditis elegans, GI17554704, Length=314, Percent_Identity=46.4968152866242, Blast_Score=281, Evalue=3e-76,
Organism=Caenorhabditis elegans, GI17570245, Length=343, Percent_Identity=34.1107871720117, Blast_Score=182, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6319403, Length=322, Percent_Identity=44.0993788819876, Blast_Score=254, Evalue=9e-69,
Organism=Saccharomyces cerevisiae, GI6320946, Length=322, Percent_Identity=42.8571428571429, Blast_Score=251, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6321776, Length=318, Percent_Identity=43.0817610062893, Blast_Score=243, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6322667, Length=198, Percent_Identity=41.9191919191919, Blast_Score=155, Evalue=9e-39,
Organism=Saccharomyces cerevisiae, GI6324511, Length=112, Percent_Identity=41.9642857142857, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21355239, Length=314, Percent_Identity=47.7707006369427, Blast_Score=286, Evalue=1e-77,
Organism=Drosophila melanogaster, GI45551540, Length=337, Percent_Identity=44.8071216617211, Blast_Score=275, Evalue=4e-74,
Organism=Drosophila melanogaster, GI24651458, Length=358, Percent_Identity=34.0782122905028, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24651456, Length=358, Percent_Identity=34.0782122905028, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI281362873, Length=358, Percent_Identity=34.0782122905028, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24651454, Length=358, Percent_Identity=34.0782122905028, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI45552010, Length=206, Percent_Identity=38.3495145631068, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24651462, Length=206, Percent_Identity=38.3495145631068, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24651464, Length=206, Percent_Identity=38.3495145631068, Blast_Score=136, Evalue=2e-32,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 35530; Mature: 35398

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYSNLKLFSLSSNHELAQKVAKEIGIELGKVSVGAHSDGETVVHIDESVRGDHVFILQS
CCCCCEEEEEECCCHHHHHHHHHHHCCEECEEEECCCCCCCEEEEECCCCCCCEEEEEEC
TSDPVNDNLMELLIMMDALRRASAASINIVLPYYGYARQDRKARAREPITSKLVANMLQI
CCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHH
AGADRLITFDLHAPQIQGFFNIPVDHLMGSPLIAEYFRRQLVSAGDDIVVVSPDHGGVGR
CCCCEEEEEECCCCCCCCEECCCHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCHH
ARKLANFLKAPLAIIDKRRPRANVAEIMNIIGDVQGKKCILIDDMIDTAGTITLAANALK
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEEECHHCCCCHHHHHHHHHH
ELGATEVYASCTHAVLSGPAIERINNSAITKLVVLDTIEMPEERQSEKIVQLSIAHLLAD
HCCCHHHHHHHHHHHHCCHHHHHCCCHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
AIIRIHERRPLSPLFELHLPSEQI
HHHHHHCCCCCCHHHHCCCCCCCC
>Mature Secondary Structure 
SYSNLKLFSLSSNHELAQKVAKEIGIELGKVSVGAHSDGETVVHIDESVRGDHVFILQS
CCCCEEEEEECCCHHHHHHHHHHHCCEECEEEECCCCCCCEEEEECCCCCCCEEEEEEC
TSDPVNDNLMELLIMMDALRRASAASINIVLPYYGYARQDRKARAREPITSKLVANMLQI
CCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHH
AGADRLITFDLHAPQIQGFFNIPVDHLMGSPLIAEYFRRQLVSAGDDIVVVSPDHGGVGR
CCCCEEEEEECCCCCCCCEECCCHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCHH
ARKLANFLKAPLAIIDKRRPRANVAEIMNIIGDVQGKKCILIDDMIDTAGTITLAANALK
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEEECHHCCCCHHHHHHHHHH
ELGATEVYASCTHAVLSGPAIERINNSAITKLVVLDTIEMPEERQSEKIVQLSIAHLLAD
HCCCHHHHHHHHHHHHCCHHHHHCCCHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
AIIRIHERRPLSPLFELHLPSEQI
HHHHHHCCCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]