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The map label for this gene is folE [H]

Identifier: 125624157

GI number: 125624157

Start: 1313335

End: 1314384

Strand: Reverse

Name: folE [H]

Synonym: llmg_1337

Alternate gene names: 125624157

Gene position: 1314384-1313335 (Counterclockwise)

Preceding gene: 125624158

Following gene: 125624156

Centisome position: 51.96

GC content: 33.81

Gene sequence:

>1050_bases
ATGCAAACAACTTATTTAAGCATGGGAAGTAATATTGGGGACCGTCAGTATTATTTACATGAAGCCATTCGTTTATTGGG
AAAACACCCGAAAATTATGATTGAAAAAGTATCAAATTTTTATGAAAGTTCTCCAGTTGGTGGCGTCAAACAAGATGATT
TTACAAATTTAGCTTTAAAGGTGGCCACACTACTTGAACCCTTAGAATTATTAGATTTTATTCATGAAGTTGAACTTTCT
TTAAATCGTGAGCGAAAAATTCACTGGGGACCAAGAACAATTGATATTGATATTATTTTCTATGGAAATTCAGAAATTCA
AGAAGAAAATTTGATTGTTCCACATAAAGAAGCTTTTAATCGTTTATTTGTTTTAAAGCCTATATTTGAGCTTTTAAGCA
ATGATTTTAAGTATTATGAGCCAATTCGCGAGGCGATTGCTAAACTTTCAGAAAGTGAGCAAGAACTGCATGTGATTGAA
GAAGAAAAGTCTCCAAAAAATCGTATTGAAGAGGCGGTCAAAGAAATTCTTTTTGCAGTGGGTGAGAATCCAAATCGAGA
GGGATTGCTTGAAACTCCTGCCAGAGTGGCTAAAATGTATGAAGAAATTCTTTCATCACAACGTTTGACAAACTTTAATG
AATACAAACTTTTTGAAATTGATTCTTCTAAGAATGATTCAATTGTGCTGATTAAAGATATTCCATTTTATTCAATGTGT
GAGCATCACATGCTACCATTCTTTGGAAAGGCTCATGTTGCTTATATTCCTGATGGTGGACGCATCATTGGATTATCGAA
AATTCCCCGTCTAGTTAATTATGTTTCTCGTAAACTTTCGGTGCAAGAAAATATTACTCATGATATTGCCGATATTTTGA
CGGATATTTTAAAACCCAAAGGAGTGGCTGTTCTTGTTGAGGGACGTCACATGTGCGTGGAAATGCGCGGTGTGAAAAAA
GTAAATTCGTTGACCAAAACTTCTTATTTCTTAGGTGAATTTAAAGAAAACGGCGAAAAAAGAATGGAATTTTTAGAAAG
TCTTTTATGA

Upstream 100 bases:

>100_bases
GAAATCGAGATGAGAGGGTAGATTTATCTGCTAAGATAAACATTTTACAGACAAAGTAGGAGAACATAATCATAAAAGAT
TGTGTCTAAATGGAGGACTT

Downstream 100 bases:

>100_bases
AAATCTTAGAACTTAATCAAGAATCTTTTTCTTTAAAAAATATTGTGATAAAATTTGAAGAATTAAGTAATGATGAATTG
ACAGGCTTACAAAAAAAGCT

Product: FolE protein

Products: NA

Alternate protein names: 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase; 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8 dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK; GTP cyclohydrolase 1; GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MQTTYLSMGSNIGDRQYYLHEAIRLLGKHPKIMIEKVSNFYESSPVGGVKQDDFTNLALKVATLLEPLELLDFIHEVELS
LNRERKIHWGPRTIDIDIIFYGNSEIQEENLIVPHKEAFNRLFVLKPIFELLSNDFKYYEPIREAIAKLSESEQELHVIE
EEKSPKNRIEEAVKEILFAVGENPNREGLLETPARVAKMYEEILSSQRLTNFNEYKLFEIDSSKNDSIVLIKDIPFYSMC
EHHMLPFFGKAHVAYIPDGGRIIGLSKIPRLVNYVSRKLSVQENITHDIADILTDILKPKGVAVLVEGRHMCVEMRGVKK
VNSLTKTSYFLGEFKENGEKRMEFLESLL

Sequences:

>Translated_349_residues
MQTTYLSMGSNIGDRQYYLHEAIRLLGKHPKIMIEKVSNFYESSPVGGVKQDDFTNLALKVATLLEPLELLDFIHEVELS
LNRERKIHWGPRTIDIDIIFYGNSEIQEENLIVPHKEAFNRLFVLKPIFELLSNDFKYYEPIREAIAKLSESEQELHVIE
EEKSPKNRIEEAVKEILFAVGENPNREGLLETPARVAKMYEEILSSQRLTNFNEYKLFEIDSSKNDSIVLIKDIPFYSMC
EHHMLPFFGKAHVAYIPDGGRIIGLSKIPRLVNYVSRKLSVQENITHDIADILTDILKPKGVAVLVEGRHMCVEMRGVKK
VNSLTKTSYFLGEFKENGEKRMEFLESLL
>Mature_349_residues
MQTTYLSMGSNIGDRQYYLHEAIRLLGKHPKIMIEKVSNFYESSPVGGVKQDDFTNLALKVATLLEPLELLDFIHEVELS
LNRERKIHWGPRTIDIDIIFYGNSEIQEENLIVPHKEAFNRLFVLKPIFELLSNDFKYYEPIREAIAKLSESEQELHVIE
EEKSPKNRIEEAVKEILFAVGENPNREGLLETPARVAKMYEEILSSQRLTNFNEYKLFEIDSSKNDSIVLIKDIPFYSMC
EHHMLPFFGKAHVAYIPDGGRIIGLSKIPRLVNYVSRKLSVQENITHDIADILTDILKPKGVAVLVEGRHMCVEMRGVKK
VNSLTKTSYFLGEFKENGEKRMEFLESLL

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=243, Percent_Identity=39.5061728395062, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI4503949, Length=243, Percent_Identity=39.5061728395062, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI66932972, Length=211, Percent_Identity=36.0189573459716, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI66932970, Length=206, Percent_Identity=36.4077669902913, Blast_Score=118, Evalue=7e-27,
Organism=Escherichia coli, GI1788476, Length=189, Percent_Identity=37.5661375661376, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1786335, Length=132, Percent_Identity=40.9090909090909, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17560486, Length=176, Percent_Identity=43.1818181818182, Blast_Score=154, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6321706, Length=185, Percent_Identity=45.945945945946, Blast_Score=159, Evalue=8e-40,
Organism=Saccharomyces cerevisiae, GI6324073, Length=136, Percent_Identity=37.5, Blast_Score=92, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24656782, Length=197, Percent_Identity=42.6395939086294, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24656796, Length=197, Percent_Identity=42.6395939086294, Blast_Score=141, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24656791, Length=197, Percent_Identity=42.6395939086294, Blast_Score=141, Evalue=6e-34,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- InterPro:   IPR000550 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI; PF01288 HPPK [H]

EC number: =2.7.6.3; =3.5.4.16 [H]

Molecular weight: Translated: 40262; Mature: 40262

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00794 HPPK ; PS00859 GTP_CYCLOHYDROL_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTTYLSMGSNIGDRQYYLHEAIRLLGKHPKIMIEKVSNFYESSPVGGVKQDDFTNLALK
CCCEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
VATLLEPLELLDFIHEVELSLNRERKIHWGPRTIDIDIIFYGNSEIQEENLIVPHKEAFN
HHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEEEEEECCCCCCCCCCCCCCHHHHH
RLFVLKPIFELLSNDFKYYEPIREAIAKLSESEQELHVIEEEKSPKNRIEEAVKEILFAV
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHH
GENPNREGLLETPARVAKMYEEILSSQRLTNFNEYKLFEIDSSKNDSIVLIKDIPFYSMC
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEECCCHHHHH
EHHMLPFFGKAHVAYIPDGGRIIGLSKIPRLVNYVSRKLSVQENITHDIADILTDILKPK
HHCCCCCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GVAVLVEGRHMCVEMRGVKKVNSLTKTSYFLGEFKENGEKRMEFLESLL
CEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCC
>Mature Secondary Structure
MQTTYLSMGSNIGDRQYYLHEAIRLLGKHPKIMIEKVSNFYESSPVGGVKQDDFTNLALK
CCCEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
VATLLEPLELLDFIHEVELSLNRERKIHWGPRTIDIDIIFYGNSEIQEENLIVPHKEAFN
HHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEEEEEECCCCCCCCCCCCCCHHHHH
RLFVLKPIFELLSNDFKYYEPIREAIAKLSESEQELHVIEEEKSPKNRIEEAVKEILFAV
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHH
GENPNREGLLETPARVAKMYEEILSSQRLTNFNEYKLFEIDSSKNDSIVLIKDIPFYSMC
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEECCCHHHHH
EHHMLPFFGKAHVAYIPDGGRIIGLSKIPRLVNYVSRKLSVQENITHDIADILTDILKPK
HHCCCCCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GVAVLVEGRHMCVEMRGVKKVNSLTKTSYFLGEFKENGEKRMEFLESLL
CEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]