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The map label for this gene is ilvD [H]

Identifier: 125624104

GI number: 125624104

Start: 1250090

End: 1251802

Strand: Reverse

Name: ilvD [H]

Synonym: llmg_1280

Alternate gene names: 125624104

Gene position: 1251802-1250090 (Counterclockwise)

Preceding gene: 125624105

Following gene: 125624103

Centisome position: 49.49

GC content: 43.61

Gene sequence:

>1713_bases
ATGGAATTCAAATATAACGGAAAAGTTGAATCAATAGAGCTCAATAAGTATTCAAAAACATTGACACAAGACCCAACACA
GCCAGCGACCCAAGCCATGTACTATGGCATTGGTTTTAAAGATGAGGACTTCAAAAAAGCTCAGGTCGGAATCGTCAGCA
TGGATTGGGACGGAAATCCCTGTAACATGCACTTGGGGACACTTGGTAGTAAAATCAAAAATTCTGTCAATCAAACTGAC
GGACTGATTGGACTTCAATTTCACACGATTGGGGTTTCTGATGGGATTGCCAACGGAAAACTTGGCATGAGATATTCCTT
GGTCAGTCGTGAAGTTATTGCTGACAGCATTGAAACCAACGCTGGTGCCGAATATTACGATGCAATTGTCGCTGTTCCCG
GTTGTGACAAAAATATGCCAGGCTCAATTATCGGTATGGCTCGGCTCAATCGTCCGTCAATTATGGTTTATGGTGGAACG
ATTGAACATGGCGAATACAAAGGCGAAAAGTTAAATATTGTTTCGGCCTTTGAAGCTCTGGGGCAAAAAATCACTGGAAA
TATTTCTGATGAAGATTATCATGGCGTTATTTGCAATGCCATTCCAGGACAAGGTGCTTGCGGAGGAATGTACACTGCCA
ATACCCTGGCTGCTGCTATTGAAACTTTAGGAATGAGTTTACCTTATTCATCTTCAAATCCAGCGGTCAGTCAAGAAAAA
GAAGACGAATGTGATGAAATTGGTCTGGCAATCAAAAATTTGCTAGAAAAAGACATCAAGCCAAGCGATATCATGACCAA
GGAAGCTTTTGAAAATGCCATAACGATCGTCATGGTTCTCGGTGGTTCAACTAATGCTGTGCTTCATATCATTGCCATGG
CTAATGCCATCGGTGTCGAAATTACGCAAGATGATTTTCAACGTATTTCCGATGTCACGCCTGTGCTTGGCGACTTCAAG
CCAAGTGGCAAGTACATGGTGGAAGATTTGCACAAAATTGGTGGCGTGCCTGCTGTTTTGAAATATTTGCTCAAAGAGGG
CAAGCTTCACGGCGACTGTTTGACGGTTACTGGTAAAACTCTAGCTGAAAATGTTAAAACAGCACTGGATTTGGACTTTG
AAAGCCAAGACATTATGCGACCACTTGAAAATCCAATCAAAGCAACAGGTCATTTGCAAATTCTTTATGGCAATCTTGCT
GAAGGTGGTTCTGTGGCAAAAATTTCTGGAAAAGAAGGCGAATTTTTTAAAGGAACGGCCCGTGTCTTTGATGGCGAACA
ACATTTTATTGACGGAATTGAGTCAGGTCGTTTGCACGCAGGAGATGTCGCTGTCATCCGTAATATCGGTCCAGTCGGAG
GCCCAGGAATGCCAGAAATGTTAAAACCGACCTCAGCCTTGATTGGAGCAGGTCTTGGAAAATCTTGTGCTTTGATTACT
GACGGACGTTTCTCTGGTGGCACACATGGCTTTGTGGTTGGCCACATTGTCCCCGAAGCGGTTGAGGGCGGATTGATTGG
CTTGGTCGAAGATGATGATATTATCGAGATTGATGCGGTCAATAACAGCATTAGTTTGAAGGTTGCTGACGATGAGATTG
CTAGACGACGTGCCAATTATCAAAAACCTGCTCCTAAAGCAACGCGTGGGGTTCTTGCTAAATTTGCTAAACTCACGCGC
CCAGCCAGTGAAGGCTGTGTGACTGATTTATAG

Upstream 100 bases:

>100_bases
TATAGACTTTATTGAATGTTCTGACAAATTATTGTATTTTCAATTTTTTAATGATAAAATAACTCTATAAAAATTTACGG
GGAGGTCAAAAAGATAACAT

Downstream 100 bases:

>100_bases
AAAGAAAGGATTGCATGAACAAAATAAAGTTAGAAAAACCAACTTCTGGCTCTCAGCTCGTCCTTGAAACTCTAAAAAAT
CTTGGAGTAGAGATTATATT

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MEFKYNGKVESIELNKYSKTLTQDPTQPATQAMYYGIGFKDEDFKKAQVGIVSMDWDGNPCNMHLGTLGSKIKNSVNQTD
GLIGLQFHTIGVSDGIANGKLGMRYSLVSREVIADSIETNAGAEYYDAIVAVPGCDKNMPGSIIGMARLNRPSIMVYGGT
IEHGEYKGEKLNIVSAFEALGQKITGNISDEDYHGVICNAIPGQGACGGMYTANTLAAAIETLGMSLPYSSSNPAVSQEK
EDECDEIGLAIKNLLEKDIKPSDIMTKEAFENAITIVMVLGGSTNAVLHIIAMANAIGVEITQDDFQRISDVTPVLGDFK
PSGKYMVEDLHKIGGVPAVLKYLLKEGKLHGDCLTVTGKTLAENVKTALDLDFESQDIMRPLENPIKATGHLQILYGNLA
EGGSVAKISGKEGEFFKGTARVFDGEQHFIDGIESGRLHAGDVAVIRNIGPVGGPGMPEMLKPTSALIGAGLGKSCALIT
DGRFSGGTHGFVVGHIVPEAVEGGLIGLVEDDDIIEIDAVNNSISLKVADDEIARRRANYQKPAPKATRGVLAKFAKLTR
PASEGCVTDL

Sequences:

>Translated_570_residues
MEFKYNGKVESIELNKYSKTLTQDPTQPATQAMYYGIGFKDEDFKKAQVGIVSMDWDGNPCNMHLGTLGSKIKNSVNQTD
GLIGLQFHTIGVSDGIANGKLGMRYSLVSREVIADSIETNAGAEYYDAIVAVPGCDKNMPGSIIGMARLNRPSIMVYGGT
IEHGEYKGEKLNIVSAFEALGQKITGNISDEDYHGVICNAIPGQGACGGMYTANTLAAAIETLGMSLPYSSSNPAVSQEK
EDECDEIGLAIKNLLEKDIKPSDIMTKEAFENAITIVMVLGGSTNAVLHIIAMANAIGVEITQDDFQRISDVTPVLGDFK
PSGKYMVEDLHKIGGVPAVLKYLLKEGKLHGDCLTVTGKTLAENVKTALDLDFESQDIMRPLENPIKATGHLQILYGNLA
EGGSVAKISGKEGEFFKGTARVFDGEQHFIDGIESGRLHAGDVAVIRNIGPVGGPGMPEMLKPTSALIGAGLGKSCALIT
DGRFSGGTHGFVVGHIVPEAVEGGLIGLVEDDDIIEIDAVNNSISLKVADDEIARRRANYQKPAPKATRGVLAKFAKLTR
PASEGCVTDL
>Mature_570_residues
MEFKYNGKVESIELNKYSKTLTQDPTQPATQAMYYGIGFKDEDFKKAQVGIVSMDWDGNPCNMHLGTLGSKIKNSVNQTD
GLIGLQFHTIGVSDGIANGKLGMRYSLVSREVIADSIETNAGAEYYDAIVAVPGCDKNMPGSIIGMARLNRPSIMVYGGT
IEHGEYKGEKLNIVSAFEALGQKITGNISDEDYHGVICNAIPGQGACGGMYTANTLAAAIETLGMSLPYSSSNPAVSQEK
EDECDEIGLAIKNLLEKDIKPSDIMTKEAFENAITIVMVLGGSTNAVLHIIAMANAIGVEITQDDFQRISDVTPVLGDFK
PSGKYMVEDLHKIGGVPAVLKYLLKEGKLHGDCLTVTGKTLAENVKTALDLDFESQDIMRPLENPIKATGHLQILYGNLA
EGGSVAKISGKEGEFFKGTARVFDGEQHFIDGIESGRLHAGDVAVIRNIGPVGGPGMPEMLKPTSALIGAGLGKSCALIT
DGRFSGGTHGFVVGHIVPEAVEGGLIGLVEDDDIIEIDAVNNSISLKVADDEIARRRANYQKPAPKATRGVLAKFAKLTR
PASEGCVTDL

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=611, Percent_Identity=36.3338788870704, Blast_Score=337, Evalue=1e-93,
Organism=Escherichia coli, GI2367371, Length=460, Percent_Identity=33.9130434782609, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1788157, Length=471, Percent_Identity=30.7855626326964, Blast_Score=176, Evalue=5e-45,
Organism=Escherichia coli, GI1786464, Length=498, Percent_Identity=31.7269076305221, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6322476, Length=565, Percent_Identity=58.0530973451327, Blast_Score=648, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 60659; Mature: 60659

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00886 ILVD_EDD_1 ; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFKYNGKVESIELNKYSKTLTQDPTQPATQAMYYGIGFKDEDFKKAQVGIVSMDWDGNP
CCEEECCEEEEEECCHHHHHHCCCCCCHHHHHEEEECCCCCCCCCEEEEEEEEECCCCCC
CNMHLGTLGSKIKNSVNQTDGLIGLQFHTIGVSDGIANGKLGMRYSLVSREVIADSIETN
CEEEHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCEEEHHHHHHHHHHHHCCC
AGAEYYDAIVAVPGCDKNMPGSIIGMARLNRPSIMVYGGTIEHGEYKGEKLNIVSAFEAL
CCCCEEEEEEECCCCCCCCCCCEEEEEECCCCEEEEECCEEECCCCCCCEEHHHHHHHHH
GQKITGNISDEDYHGVICNAIPGQGACGGMYTANTLAAAIETLGMSLPYSSSNPAVSQEK
CHHHCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
EDECDEIGLAIKNLLEKDIKPSDIMTKEAFENAITIVMVLGGSTNAVLHIIAMANAIGVE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEEEEECCCCHHHEEHEEHHHHHCEE
ITQDDFQRISDVTPVLGDFKPSGKYMVEDLHKIGGVPAVLKYLLKEGKLHGDCLTVTGKT
ECHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEECCHH
LAENVKTALDLDFESQDIMRPLENPIKATGHLQILYGNLAEGGSVAKISGKEGEFFKGTA
HHHHHHHHEECCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEECCCCCCEEECHH
RVFDGEQHFIDGIESGRLHAGDVAVIRNIGPVGGPGMPEMLKPTSALIGAGLGKSCALIT
HEECCHHHHHCCCCCCCEECCCEEEEECCCCCCCCCCHHHHCHHHHHHHCCCCCCEEEEE
DGRFSGGTHGFVVGHIVPEAVEGGLIGLVEDDDIIEIDAVNNSISLKVADDEIARRRANY
CCCCCCCCCCEEEEEECHHHHCCCEEEEECCCCEEEEEECCCEEEEEECCHHHHHHHCCC
QKPAPKATRGVLAKFAKLTRPASEGCVTDL
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MEFKYNGKVESIELNKYSKTLTQDPTQPATQAMYYGIGFKDEDFKKAQVGIVSMDWDGNP
CCEEECCEEEEEECCHHHHHHCCCCCCHHHHHEEEECCCCCCCCCEEEEEEEEECCCCCC
CNMHLGTLGSKIKNSVNQTDGLIGLQFHTIGVSDGIANGKLGMRYSLVSREVIADSIETN
CEEEHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCEEEHHHHHHHHHHHHCCC
AGAEYYDAIVAVPGCDKNMPGSIIGMARLNRPSIMVYGGTIEHGEYKGEKLNIVSAFEAL
CCCCEEEEEEECCCCCCCCCCCEEEEEECCCCEEEEECCEEECCCCCCCEEHHHHHHHHH
GQKITGNISDEDYHGVICNAIPGQGACGGMYTANTLAAAIETLGMSLPYSSSNPAVSQEK
CHHHCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
EDECDEIGLAIKNLLEKDIKPSDIMTKEAFENAITIVMVLGGSTNAVLHIIAMANAIGVE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEEEEECCCCHHHEEHEEHHHHHCEE
ITQDDFQRISDVTPVLGDFKPSGKYMVEDLHKIGGVPAVLKYLLKEGKLHGDCLTVTGKT
ECHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEECCHH
LAENVKTALDLDFESQDIMRPLENPIKATGHLQILYGNLAEGGSVAKISGKEGEFFKGTA
HHHHHHHHEECCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEECCCCCCEEECHH
RVFDGEQHFIDGIESGRLHAGDVAVIRNIGPVGGPGMPEMLKPTSALIGAGLGKSCALIT
HEECCHHHHHCCCCCCCEECCCEEEEECCCCCCCCCCHHHHCHHHHHHHCCCCCCEEEEE
DGRFSGGTHGFVVGHIVPEAVEGGLIGLVEDDDIIEIDAVNNSISLKVADDEIARRRANY
CCCCCCCCCCEEEEEECHHHHCCCEEEEECCCCEEEEEECCCEEEEEECCHHHHHHHCCC
QKPAPKATRGVLAKFAKLTRPASEGCVTDL
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1400210; 11337471 [H]