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The map label for this gene is ilvB [H]

Identifier: 125624103

GI number: 125624103

Start: 1248351

End: 1250075

Strand: Reverse

Name: ilvB [H]

Synonym: llmg_1279

Alternate gene names: 125624103

Gene position: 1250075-1248351 (Counterclockwise)

Preceding gene: 125624104

Following gene: 125624102

Centisome position: 49.42

GC content: 41.22

Gene sequence:

>1725_bases
ATGAACAAAATAAAGTTAGAAAAACCAACTTCTGGCTCTCAGCTCGTCCTTGAAACTCTAAAAAATCTTGGAGTAGAGAT
TATATTTGGCTACCCCGGTGGGGCTATGCTTCCTTTATATGACGCGATTCATAGTTTTGAAGGAATTAAGCACATCTTAG
CTCGTCATGAGCAAGGGGCGACTCATGAGGCTGAAGGTTACGCCAAATCTTCTGGAAAAGTTGGAGTGGTTGTGGTTACT
TCTGGGCCGGGCGCAACTAATGCGGTGACGGGAATTGCTGATGCTTATCTTGATTCCGTTCCACTGTTAGTTTTTACTGG
ACAAGTCGGACGCCAATCCATTGGTAAGGATGCATTTCAAGAAGCTGATACTGTTGGCATTACCGCACCCATCACAAAAT
ATAATTATCAAATCAGAGATACAGCCGATATTCCAAGAATCGTGACCGAAGCCTATTACTTGGCCCAAACAGGGCGTCCC
GGACCGGTAGAAATTGATTTACCTAAGGATGTTTCAACACTTGAAGTCACTGAAATCAATGACCCAACAATAAACCTGCC
TCATTACAGGCCCACTGAAAGTGCAAACAATCATCAATTGAACGAATTACTGACAAAACTTTCTGTCAGTAAATGTCCAG
TCCTTATTGCGGGTGGTGGCATTAATTATTCAGATTCTGTCAGTGAATTTCGTGAATTTGTTGAAAAATATCAAATCCCC
GTGGTTTCAACTTTACTAGGTTTAGGAACTTTGCCGATTGATCACGAATTGCAATTGGGTATGGCAGGAATGCATGGTTC
TTATGCAGCCAATATGGCTCTGGTCAAAGCTGATTATATTATCAACTTTGGCTCCCGTTTTGATGATCGAGTTGTTTCCA
ATCCATTAAAATTCGCTGAGAATGCAGTCGTTGCTCATATTGATATTGACGCTGCCGAACTTGGAAAAATCGTAAAAACA
GATATTCCCATTCATTCAGATTTGAAAGATGCACTGACGAAACTTCTTAATTTTCCTAAAGTTGATACAGATTTTACTGA
CTGGGTTCAATTGGTCACAGAAAACAAGAAAAAAGTACCATTTGCTTACGAAACGCCACAAGATGAAATTCGTCCACAAG
AAGCTATCGAATTAATTGGAAAATATACTGACGGAAACTCAATTATCGTTACTGACGTTGGTCAGCACCAGATGTGGGCG
GCGCAATATTATCCTTACAAAAACGCTCGTCAGCTGATTACGTCTGGCGGAATGGGAACAATGGGCTTTGGCATCCCAGC
GGCAATTGGTGCCAAACTGGCCAATCCTGATAAAAATGTCATCGTTTTTGTCGGAGATGGTGGTTTTCAAATGACCAATC
AAGAACTGGCTTTACTTAATGGTTATGGCATTGCAATCAAAGTGGTGTTGATTAATAATCATTCGCTAGGAATGGTTCGT
CAGTGGCAAGAATCTTTCTATGAGGAGCGCCGCTCACAATCCGTTTTTGATGCCGAACCCAATTTTCAATTATTGGCCGA
GGCGTACGGAATCAAACACTTGAAGCTAGAAAATCCCAAGACTTTGGCTGAAGATTTGAAAATCATTACAAAAGATGAAC
CAATGCTTATCGAAATTGTCATTTCAAAATCTGAACATGTTTTGCCAATGATACCGGCAGGATTGCATAATGACGAGATG
ATTGGTCTGACTTTTACTGACGATAGGGAGGTGGACAATGCGTAG

Upstream 100 bases:

>100_bases
CTGCTCCTAAAGCAACGCGTGGGGTTCTTGCTAAATTTGCTAAACTCACGCGCCCAGCCAGTGAAGGCTGTGTGACTGAT
TTATAGAAAGAAAGGATTGC

Downstream 100 bases:

>100_bases
AATGATTATTGCAAAGCTTCATAATGTCACAGGAATCATGAATCGTTTTACGGCTGTACTCAACCGTAGGCAGGTAAATA
TCATCTCAATTACCGCTGGT

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MNKIKLEKPTSGSQLVLETLKNLGVEIIFGYPGGAMLPLYDAIHSFEGIKHILARHEQGATHEAEGYAKSSGKVGVVVVT
SGPGATNAVTGIADAYLDSVPLLVFTGQVGRQSIGKDAFQEADTVGITAPITKYNYQIRDTADIPRIVTEAYYLAQTGRP
GPVEIDLPKDVSTLEVTEINDPTINLPHYRPTESANNHQLNELLTKLSVSKCPVLIAGGGINYSDSVSEFREFVEKYQIP
VVSTLLGLGTLPIDHELQLGMAGMHGSYAANMALVKADYIINFGSRFDDRVVSNPLKFAENAVVAHIDIDAAELGKIVKT
DIPIHSDLKDALTKLLNFPKVDTDFTDWVQLVTENKKKVPFAYETPQDEIRPQEAIELIGKYTDGNSIIVTDVGQHQMWA
AQYYPYKNARQLITSGGMGTMGFGIPAAIGAKLANPDKNVIVFVGDGGFQMTNQELALLNGYGIAIKVVLINNHSLGMVR
QWQESFYEERRSQSVFDAEPNFQLLAEAYGIKHLKLENPKTLAEDLKIITKDEPMLIEIVISKSEHVLPMIPAGLHNDEM
IGLTFTDDREVDNA

Sequences:

>Translated_574_residues
MNKIKLEKPTSGSQLVLETLKNLGVEIIFGYPGGAMLPLYDAIHSFEGIKHILARHEQGATHEAEGYAKSSGKVGVVVVT
SGPGATNAVTGIADAYLDSVPLLVFTGQVGRQSIGKDAFQEADTVGITAPITKYNYQIRDTADIPRIVTEAYYLAQTGRP
GPVEIDLPKDVSTLEVTEINDPTINLPHYRPTESANNHQLNELLTKLSVSKCPVLIAGGGINYSDSVSEFREFVEKYQIP
VVSTLLGLGTLPIDHELQLGMAGMHGSYAANMALVKADYIINFGSRFDDRVVSNPLKFAENAVVAHIDIDAAELGKIVKT
DIPIHSDLKDALTKLLNFPKVDTDFTDWVQLVTENKKKVPFAYETPQDEIRPQEAIELIGKYTDGNSIIVTDVGQHQMWA
AQYYPYKNARQLITSGGMGTMGFGIPAAIGAKLANPDKNVIVFVGDGGFQMTNQELALLNGYGIAIKVVLINNHSLGMVR
QWQESFYEERRSQSVFDAEPNFQLLAEAYGIKHLKLENPKTLAEDLKIITKDEPMLIEIVISKSEHVLPMIPAGLHNDEM
IGLTFTDDREVDNA
>Mature_574_residues
MNKIKLEKPTSGSQLVLETLKNLGVEIIFGYPGGAMLPLYDAIHSFEGIKHILARHEQGATHEAEGYAKSSGKVGVVVVT
SGPGATNAVTGIADAYLDSVPLLVFTGQVGRQSIGKDAFQEADTVGITAPITKYNYQIRDTADIPRIVTEAYYLAQTGRP
GPVEIDLPKDVSTLEVTEINDPTINLPHYRPTESANNHQLNELLTKLSVSKCPVLIAGGGINYSDSVSEFREFVEKYQIP
VVSTLLGLGTLPIDHELQLGMAGMHGSYAANMALVKADYIINFGSRFDDRVVSNPLKFAENAVVAHIDIDAAELGKIVKT
DIPIHSDLKDALTKLLNFPKVDTDFTDWVQLVTENKKKVPFAYETPQDEIRPQEAIELIGKYTDGNSIIVTDVGQHQMWA
AQYYPYKNARQLITSGGMGTMGFGIPAAIGAKLANPDKNVIVFVGDGGFQMTNQELALLNGYGIAIKVVLINNHSLGMVR
QWQESFYEERRSQSVFDAEPNFQLLAEAYGIKHLKLENPKTLAEDLKIITKDEPMLIEIVISKSEHVLPMIPAGLHNDEM
IGLTFTDDREVDNA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=568, Percent_Identity=26.7605633802817, Blast_Score=169, Evalue=8e-42,
Organism=Homo sapiens, GI21361361, Length=493, Percent_Identity=26.5720081135903, Blast_Score=141, Evalue=2e-33,
Organism=Escherichia coli, GI87081685, Length=562, Percent_Identity=43.4163701067616, Blast_Score=499, Evalue=1e-142,
Organism=Escherichia coli, GI1790104, Length=558, Percent_Identity=43.1899641577061, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI1786717, Length=553, Percent_Identity=32.7305605786618, Blast_Score=311, Evalue=6e-86,
Organism=Escherichia coli, GI1787096, Length=539, Percent_Identity=26.7161410018553, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1788716, Length=492, Percent_Identity=25, Blast_Score=126, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17531299, Length=573, Percent_Identity=26.3525305410122, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17531301, Length=573, Percent_Identity=26.3525305410122, Blast_Score=145, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17542570, Length=481, Percent_Identity=25.7796257796258, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6323755, Length=585, Percent_Identity=41.3675213675214, Blast_Score=436, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6320816, Length=494, Percent_Identity=24.6963562753036, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6320123, Length=574, Percent_Identity=22.2996515679443, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323163, Length=479, Percent_Identity=24.6346555323591, Blast_Score=100, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6321524, Length=489, Percent_Identity=26.5848670756646, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323073, Length=495, Percent_Identity=24.4444444444444, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19922626, Length=574, Percent_Identity=25.4355400696864, Blast_Score=155, Evalue=7e-38,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62849; Mature: 62849

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIKLEKPTSGSQLVLETLKNLGVEIIFGYPGGAMLPLYDAIHSFEGIKHILARHEQGA
CCCEECCCCCCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
THEAEGYAKSSGKVGVVVVTSGPGATNAVTGIADAYLDSVPLLVFTGQVGRQSIGKDAFQ
CCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHCCHHHHH
EADTVGITAPITKYNYQIRDTADIPRIVTEAYYLAQTGRPGPVEIDLPKDVSTLEVTEIN
HCCCCEEEECEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEECC
DPTINLPHYRPTESANNHQLNELLTKLSVSKCPVLIAGGGINYSDSVSEFREFVEKYQIP
CCEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHCCC
VVSTLLGLGTLPIDHELQLGMAGMHGSYAANMALVKADYIINFGSRFDDRVVSNPLKFAE
HHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCEEEEEEEEEEECCCCCCHHHHCCCHHHCC
NAVVAHIDIDAAELGKIVKTDIPIHSDLKDALTKLLNFPKVDTDFTDWVQLVTENKKKVP
CEEEEEEECCHHHHCHHHCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCC
FAYETPQDEIRPQEAIELIGKYTDGNSIIVTDVGQHQMWAAQYYPYKNARQLITSGGMGT
EEECCCHHHCCHHHHHHHHHEECCCCEEEEEECCCCCEEEHHCCCCHHHHHHHHCCCCCC
MGFGIPAAIGAKLANPDKNVIVFVGDGGFQMTNQELALLNGYGIAIKVVLINNHSLGMVR
CCCCCHHHHCCCCCCCCCCEEEEECCCCCEECCCCEEEEECCCEEEEEEEEECCCCHHHH
QWQESFYEERRSQSVFDAEPNFQLLAEAYGIKHLKLENPKTLAEDLKIITKDEPMLIEIV
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHCEEEEEECCCHHHHHHHEEEECCCCEEEEEE
ISKSEHVLPMIPAGLHNDEMIGLTFTDDREVDNA
ECCCCCEEEECCCCCCCCCEEEEEECCCCCCCCC
>Mature Secondary Structure
MNKIKLEKPTSGSQLVLETLKNLGVEIIFGYPGGAMLPLYDAIHSFEGIKHILARHEQGA
CCCEECCCCCCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
THEAEGYAKSSGKVGVVVVTSGPGATNAVTGIADAYLDSVPLLVFTGQVGRQSIGKDAFQ
CCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHCCHHHHH
EADTVGITAPITKYNYQIRDTADIPRIVTEAYYLAQTGRPGPVEIDLPKDVSTLEVTEIN
HCCCCEEEECEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEECC
DPTINLPHYRPTESANNHQLNELLTKLSVSKCPVLIAGGGINYSDSVSEFREFVEKYQIP
CCEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHCCC
VVSTLLGLGTLPIDHELQLGMAGMHGSYAANMALVKADYIINFGSRFDDRVVSNPLKFAE
HHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCEEEEEEEEEEECCCCCCHHHHCCCHHHCC
NAVVAHIDIDAAELGKIVKTDIPIHSDLKDALTKLLNFPKVDTDFTDWVQLVTENKKKVP
CEEEEEEECCHHHHCHHHCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCC
FAYETPQDEIRPQEAIELIGKYTDGNSIIVTDVGQHQMWAAQYYPYKNARQLITSGGMGT
EEECCCHHHCCHHHHHHHHHEECCCCEEEEEECCCCCEEEHHCCCCHHHHHHHHCCCCCC
MGFGIPAAIGAKLANPDKNVIVFVGDGGFQMTNQELALLNGYGIAIKVVLINNHSLGMVR
CCCCCHHHHCCCCCCCCCCEEEEECCCCCEECCCCEEEEECCCEEEEEEEEECCCCHHHH
QWQESFYEERRSQSVFDAEPNFQLLAEAYGIKHLKLENPKTLAEDLKIITKDEPMLIEIV
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHCEEEEEECCCHHHHHHHEEEECCCCEEEEEE
ISKSEHVLPMIPAGLHNDEMIGLTFTDDREVDNA
ECCCCCEEEECCCCCCCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1400210; 11337471 [H]