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The map label for this gene is purD [H]

Identifier: 125623834

GI number: 125623834

Start: 963606

End: 964847

Strand: Direct

Name: purD [H]

Synonym: llmg_0997

Alternate gene names: 125623834

Gene position: 963606-964847 (Clockwise)

Preceding gene: 125623832

Following gene: 125623835

Centisome position: 38.1

GC content: 38.97

Gene sequence:

>1242_bases
ATGAAAATTTTGGTAATTGGCTCAGGTGGCCGTGAACATGCTCTGGCAAAAAAATTTATGGAAAGCCCTCAAGTTGAAAA
AGTATTTGTTGCTCCAGGCAACTCAGGAATGAAAAAAGATGGAATTCAAATAGTTGGTATTTCCGAACTTTCAAATGATG
AATTGGTTGAATTTGCTCAAAAAGAGAACATTGACTTAAGTTTTGTCGGACCAGAAACAGCGCTTATGAATGGTGTAGTC
GATGCTTTTGAAGAGAAAAATCTAATTATCTTTGGACCAAGAAAAAAAGCTGCCGAACTTGAAGGCTCTAAAGATTTTGC
AAAGTCAATCATGAAAAAATATGGTGTTCCTACGGCAGATTATGCAACTTTTGATTCTTTAGAACCAGCGCTCTCACATC
TTAAACAACATGGGGTTCCCATTGTGATTAAAGCAGATGGATTAGCGGCCGGAAAAGGAGTAACAGTTGCTTTTGATATT
GCAAGTGCGCAAGCAGCATTAGCAGATATATTTTCTGGAAGCCAAGGGAAAGTTGTGATTGAAGAGTTTCTTGATGGGGA
GGAGTTTTCTTTATTTAGTTTTGTTCATCAAGGAAAGATTTATCCAATGCCTATTGCTCAAGACCATAAACGTGCTTTTG
ATGGTGATAAAGGACCAAATACAGGTGGTATGGGGGCCTACTCCCCAGTTCCTCACCTTCCTAAAGAAGTGATAAATGAA
GCTTTAGAAAAAGTTGTGAAACCCACGGTTGCGGGATTGATTAACGAGGGAAATTCGTTTACTGGTGTTCTTTATGCTGG
ATTGATTTTGACAGCAGATGGGGTCAAGACAATCGAATTTAATGCAAGGTTTGGTGATCCTGAAACCCAAGTTGTTCTGC
CTAGGCTTAAAAGTGATTTGGCACAGGCAATCATTGATATTTTAGCTGGAGATGTGCCAACATTAGAATGGCTTGAAACA
GGCGTAACTTTGGGAGTAGTTGTTGCCTCAGAGGGTTATCCAGCTCAAGCAAAAACAGGAAGCATTCTTCCTGATATTCC
TAAAGAGCTGAATGTCTATTATGCAGGTGTAACAGAGAATGAAGCCAATCAACTTGTAAGTAGTGGTGGACGTGTATATC
TTCTTTCTGAAAGTGGAAAAGATATCGCAAGTACGCAGAAATCACTCTATGATAAACTTGATAAATTAGATAATCAAGGG
CTCTTTTATCGGCATGACATTGGTTCAAAAGCCGTTAAATAA

Upstream 100 bases:

>100_bases
GAGCAACTAGAGAAGTGAAAAAACAATAAAATATTTAAATGAATTGCTGTATTAACTGCAATCACAAGAATTTGGAAGAA
TCGAAAATATCGGAGAAATG

Downstream 100 bases:

>100_bases
CTTAATCAAAGTTTTGAAAGATTGCTGATAGAATTTTTACTCTTGAAAAGTAGTTTAGAAATGGAGCTGACATGATTTTT
GTTTTTGACCTTGATGGTAC

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MKILVIGSGGREHALAKKFMESPQVEKVFVAPGNSGMKKDGIQIVGISELSNDELVEFAQKENIDLSFVGPETALMNGVV
DAFEEKNLIIFGPRKKAAELEGSKDFAKSIMKKYGVPTADYATFDSLEPALSHLKQHGVPIVIKADGLAAGKGVTVAFDI
ASAQAALADIFSGSQGKVVIEEFLDGEEFSLFSFVHQGKIYPMPIAQDHKRAFDGDKGPNTGGMGAYSPVPHLPKEVINE
ALEKVVKPTVAGLINEGNSFTGVLYAGLILTADGVKTIEFNARFGDPETQVVLPRLKSDLAQAIIDILAGDVPTLEWLET
GVTLGVVVASEGYPAQAKTGSILPDIPKELNVYYAGVTENEANQLVSSGGRVYLLSESGKDIASTQKSLYDKLDKLDNQG
LFYRHDIGSKAVK

Sequences:

>Translated_413_residues
MKILVIGSGGREHALAKKFMESPQVEKVFVAPGNSGMKKDGIQIVGISELSNDELVEFAQKENIDLSFVGPETALMNGVV
DAFEEKNLIIFGPRKKAAELEGSKDFAKSIMKKYGVPTADYATFDSLEPALSHLKQHGVPIVIKADGLAAGKGVTVAFDI
ASAQAALADIFSGSQGKVVIEEFLDGEEFSLFSFVHQGKIYPMPIAQDHKRAFDGDKGPNTGGMGAYSPVPHLPKEVINE
ALEKVVKPTVAGLINEGNSFTGVLYAGLILTADGVKTIEFNARFGDPETQVVLPRLKSDLAQAIIDILAGDVPTLEWLET
GVTLGVVVASEGYPAQAKTGSILPDIPKELNVYYAGVTENEANQLVSSGGRVYLLSESGKDIASTQKSLYDKLDKLDNQG
LFYRHDIGSKAVK
>Mature_413_residues
MKILVIGSGGREHALAKKFMESPQVEKVFVAPGNSGMKKDGIQIVGISELSNDELVEFAQKENIDLSFVGPETALMNGVV
DAFEEKNLIIFGPRKKAAELEGSKDFAKSIMKKYGVPTADYATFDSLEPALSHLKQHGVPIVIKADGLAAGKGVTVAFDI
ASAQAALADIFSGSQGKVVIEEFLDGEEFSLFSFVHQGKIYPMPIAQDHKRAFDGDKGPNTGGMGAYSPVPHLPKEVINE
ALEKVVKPTVAGLINEGNSFTGVLYAGLILTADGVKTIEFNARFGDPETQVVLPRLKSDLAQAIIDILAGDVPTLEWLET
GVTLGVVVASEGYPAQAKTGSILPDIPKELNVYYAGVTENEANQLVSSGGRVYLLSESGKDIASTQKSLYDKLDKLDNQG
LFYRHDIGSKAVK

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=424, Percent_Identity=41.5094339622642, Blast_Score=309, Evalue=2e-84,
Organism=Homo sapiens, GI4503915, Length=424, Percent_Identity=41.5094339622642, Blast_Score=309, Evalue=2e-84,
Organism=Homo sapiens, GI209869995, Length=424, Percent_Identity=41.5094339622642, Blast_Score=309, Evalue=2e-84,
Organism=Homo sapiens, GI209869993, Length=424, Percent_Identity=41.5094339622642, Blast_Score=309, Evalue=2e-84,
Organism=Escherichia coli, GI1790438, Length=426, Percent_Identity=47.1830985915493, Blast_Score=373, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17567511, Length=423, Percent_Identity=38.0614657210402, Blast_Score=255, Evalue=3e-68,
Organism=Saccharomyces cerevisiae, GI6321203, Length=443, Percent_Identity=38.6004514672686, Blast_Score=276, Evalue=5e-75,
Organism=Drosophila melanogaster, GI62471619, Length=431, Percent_Identity=37.3549883990719, Blast_Score=258, Evalue=7e-69,
Organism=Drosophila melanogaster, GI24582400, Length=431, Percent_Identity=37.3549883990719, Blast_Score=256, Evalue=1e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 44158; Mature: 44158

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVIGSGGREHALAKKFMESPQVEKVFVAPGNSGMKKDGIQIVGISELSNDELVEFAQ
CEEEEECCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHH
KENIDLSFVGPETALMNGVVDAFEEKNLIIFGPRKKAAELEGSKDFAKSIMKKYGVPTAD
HCCCCEEEECCHHHHHHHHHHHHCCCCEEEECCCCHHHHCCCCHHHHHHHHHHHCCCCCC
YATFDSLEPALSHLKQHGVPIVIKADGLAAGKGVTVAFDIASAQAALADIFSGSQGKVVI
CCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCEEH
EEFLDGEEFSLFSFVHQGKIYPMPIAQDHKRAFDGDKGPNTGGMGAYSPVPHLPKEVINE
HHHCCCCCHHHHHHHHCCCEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHH
ALEKVVKPTVAGLINEGNSFTGVLYAGLILTADGVKTIEFNARFGDPETQVVLPRLKSDL
HHHHHHHHHHHHHHCCCCCCHHHHHHCCCEECCCCEEEEEECCCCCCCCEEEHHHHHHHH
AQAIIDILAGDVPTLEWLETGVTLGVVVASEGYPAQAKTGSILPDIPKELNVYYAGVTEN
HHHHHHHHCCCCCHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
EANQLVSSGGRVYLLSESGKDIASTQKSLYDKLDKLDNQGLFYRHDIGSKAVK
HHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCC
>Mature Secondary Structure
MKILVIGSGGREHALAKKFMESPQVEKVFVAPGNSGMKKDGIQIVGISELSNDELVEFAQ
CEEEEECCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHH
KENIDLSFVGPETALMNGVVDAFEEKNLIIFGPRKKAAELEGSKDFAKSIMKKYGVPTAD
HCCCCEEEECCHHHHHHHHHHHHCCCCEEEECCCCHHHHCCCCHHHHHHHHHHHCCCCCC
YATFDSLEPALSHLKQHGVPIVIKADGLAAGKGVTVAFDIASAQAALADIFSGSQGKVVI
CCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCEEH
EEFLDGEEFSLFSFVHQGKIYPMPIAQDHKRAFDGDKGPNTGGMGAYSPVPHLPKEVINE
HHHCCCCCHHHHHHHHCCCEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHH
ALEKVVKPTVAGLINEGNSFTGVLYAGLILTADGVKTIEFNARFGDPETQVVLPRLKSDL
HHHHHHHHHHHHHHCCCCCCHHHHHHCCCEECCCCEEEEEECCCCCCCCEEEHHHHHHHH
AQAIIDILAGDVPTLEWLETGVTLGVVVASEGYPAQAKTGSILPDIPKELNVYYAGVTEN
HHHHHHHHCCCCCHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
EANQLVSSGGRVYLLSESGKDIASTQKSLYDKLDKLDNQGLFYRHDIGSKAVK
HHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9797284; 11337471 [H]