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The map label for this gene is purH [H]

Identifier: 125623831

GI number: 125623831

Start: 958681

End: 960237

Strand: Direct

Name: purH [H]

Synonym: llmg_0994

Alternate gene names: 125623831

Gene position: 958681-960237 (Clockwise)

Preceding gene: 125623830

Following gene: 125623832

Centisome position: 37.9

GC content: 39.56

Gene sequence:

>1557_bases
ATGTCAAAACGTGCACTTATCAGTGTTTCAGACAAAGAAGGTATTGTAGAATTTGCAAAGAAAATTCAGGAGTTAGGTTG
GGAAATTATCTCAACAGGCGGAACAAAAGCAGTTCTGGACCAAGAGGAAATTCCGAATATTGCAATTGATGAAGTAACTG
GCTTTCCAGAAATGATGGATGGTCGAGTTAAAACATTACATCCGCTGATTCATGGTGCACTCCTTGGACGTAGAGATTTG
GACAGTCACATGAAATCAATGGCTGAACATCATATTTCTCCGATTGACTTAGTTGTTGTAAATTTATATCCTTTCAAAGA
AACCTTGCTTGCTGGTGGTAGCCAAGCAGAAATGATTGAAAAAATTGATATTGGTGGGCCGTCGATGTTACGTTCGGCAG
CTAAAAACCATGCGGCAGTCACAGTTGTCTGTGACCCGATGGATTATGAAAAAGTAGAAAAAGAGCTTTCAGCAGACGGG
GAAACAAGTTTAGAACTTCGTCAGCAACTTGCAGCAAAAGTTTTTCGTCATACTGCAAGTTATGATGCCCTCATTGCGCA
ATATTTGACAGAAGAATTTCCAGTAGAAGATACAAAACCCGAAAAATTAACCTTGACTTATGACCTTAAACAAGGGATGC
GTTATGGTGAAAATCCACAACAAAGTGCTGATTTTTATGAATCAGGATTACCAACAACTTACTCTATTGCGCAAAGTCAT
CAACTTCATGGTAAAGAATTAAGTTATAACAATGTTCGTGATGCTGATGCAGCTTTGCAAATTGCAAGGGATTTTGATGA
GCCAACCGTTGTGGCCCTCAAACATATGAACCCTTGTGGGATTGGAACGGCTAAAAATATTGAACAAGCTTGGGATTATG
CTTATGAAGCGGACCCTGTTTCGATTTTTGGTGGGATTATCGTTTTAAATAGAGAAGTCACTGAGGAAACGGCTCAAAAA
ATGAGCAAGATTTTCCTTGAGATTATTATTGCTCCATCATATAGTAAAGAGGCTTTAGAAATTTTGAGTAAAAAGAAAAA
TATTCGTCTTTTAACCGTTGATTTTTCTAAAAAAGAGCAATCAGAAAAAGAAGCGCTTGTAACAGGGGTACTTGGTGGGC
TTCTTGTTCAAAATCAAGATGTCATTGTTGAAAATCCGAAAGAATGGACAGTGGCGACTAAAGTTCAACCAACTGACAGA
CAAATGGAAGCCATGAAGTTTGCTTGGAAAGCAGTAAAATTTGTCAAATCAAATGGAATCATTGTAACAAATGACCACCA
AACGCTTGGCGTTGGCCCTGGGCAAACTAATCGTGTCGGCTCGGTAAAAATTGCCTTGGAAGCGACAGCTGATAAGGACG
CCCTTCTTCAAGAAAATGCCGTACTTGGCTCAGATGCCTTTTTCCCATTTGCGGACAATATTGATGAAATTGCCAAAGCG
GGAATTAAAGCAATCGTTCAACCGGGTGGCTCTGTTCGTGACCAAGAAGTGATTGAAGCTTGTGATAAATATGGTATTGC
GATGGTCTTTACTGGACTCCGCCATTTTAGACATTAA

Upstream 100 bases:

>100_bases
AAAATAAAGACATGATAAATAGTTACTGACAGACTTATCAGTACTTTTAGCAGAAAAATCTTGACTTACAGAACGGTAAG
TAGAAAACTTGGAGGAAATC

Downstream 100 bases:

>100_bases
TTAAAAATCTGCCTTAGGGTAGAATTTTTGTCATCTACAATCTAAAAAATAATTTTCGATACTTTTTTCTGCTATAATGT
AAGGAATGCAAAGAATACAT

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MSKRALISVSDKEGIVEFAKKIQELGWEIISTGGTKAVLDQEEIPNIAIDEVTGFPEMMDGRVKTLHPLIHGALLGRRDL
DSHMKSMAEHHISPIDLVVVNLYPFKETLLAGGSQAEMIEKIDIGGPSMLRSAAKNHAAVTVVCDPMDYEKVEKELSADG
ETSLELRQQLAAKVFRHTASYDALIAQYLTEEFPVEDTKPEKLTLTYDLKQGMRYGENPQQSADFYESGLPTTYSIAQSH
QLHGKELSYNNVRDADAALQIARDFDEPTVVALKHMNPCGIGTAKNIEQAWDYAYEADPVSIFGGIIVLNREVTEETAQK
MSKIFLEIIIAPSYSKEALEILSKKKNIRLLTVDFSKKEQSEKEALVTGVLGGLLVQNQDVIVENPKEWTVATKVQPTDR
QMEAMKFAWKAVKFVKSNGIIVTNDHQTLGVGPGQTNRVGSVKIALEATADKDALLQENAVLGSDAFFPFADNIDEIAKA
GIKAIVQPGGSVRDQEVIEACDKYGIAMVFTGLRHFRH

Sequences:

>Translated_518_residues
MSKRALISVSDKEGIVEFAKKIQELGWEIISTGGTKAVLDQEEIPNIAIDEVTGFPEMMDGRVKTLHPLIHGALLGRRDL
DSHMKSMAEHHISPIDLVVVNLYPFKETLLAGGSQAEMIEKIDIGGPSMLRSAAKNHAAVTVVCDPMDYEKVEKELSADG
ETSLELRQQLAAKVFRHTASYDALIAQYLTEEFPVEDTKPEKLTLTYDLKQGMRYGENPQQSADFYESGLPTTYSIAQSH
QLHGKELSYNNVRDADAALQIARDFDEPTVVALKHMNPCGIGTAKNIEQAWDYAYEADPVSIFGGIIVLNREVTEETAQK
MSKIFLEIIIAPSYSKEALEILSKKKNIRLLTVDFSKKEQSEKEALVTGVLGGLLVQNQDVIVENPKEWTVATKVQPTDR
QMEAMKFAWKAVKFVKSNGIIVTNDHQTLGVGPGQTNRVGSVKIALEATADKDALLQENAVLGSDAFFPFADNIDEIAKA
GIKAIVQPGGSVRDQEVIEACDKYGIAMVFTGLRHFRH
>Mature_517_residues
SKRALISVSDKEGIVEFAKKIQELGWEIISTGGTKAVLDQEEIPNIAIDEVTGFPEMMDGRVKTLHPLIHGALLGRRDLD
SHMKSMAEHHISPIDLVVVNLYPFKETLLAGGSQAEMIEKIDIGGPSMLRSAAKNHAAVTVVCDPMDYEKVEKELSADGE
TSLELRQQLAAKVFRHTASYDALIAQYLTEEFPVEDTKPEKLTLTYDLKQGMRYGENPQQSADFYESGLPTTYSIAQSHQ
LHGKELSYNNVRDADAALQIARDFDEPTVVALKHMNPCGIGTAKNIEQAWDYAYEADPVSIFGGIIVLNREVTEETAQKM
SKIFLEIIIAPSYSKEALEILSKKKNIRLLTVDFSKKEQSEKEALVTGVLGGLLVQNQDVIVENPKEWTVATKVQPTDRQ
MEAMKFAWKAVKFVKSNGIIVTNDHQTLGVGPGQTNRVGSVKIALEATADKDALLQENAVLGSDAFFPFADNIDEIAKAG
IKAIVQPGGSVRDQEVIEACDKYGIAMVFTGLRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=473, Percent_Identity=34.46088794926, Blast_Score=233, Evalue=5e-61,
Organism=Escherichia coli, GI1790439, Length=538, Percent_Identity=47.7695167286245, Blast_Score=479, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI71985564, Length=614, Percent_Identity=30.1302931596091, Blast_Score=240, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI71985556, Length=62, Percent_Identity=50, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71985574, Length=309, Percent_Identity=23.6245954692557, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323768, Length=476, Percent_Identity=33.6134453781513, Blast_Score=231, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6323056, Length=495, Percent_Identity=33.1313131313131, Blast_Score=225, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24649832, Length=493, Percent_Identity=34.0770791075051, Blast_Score=232, Evalue=5e-61,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 57159; Mature: 57028

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRALISVSDKEGIVEFAKKIQELGWEIISTGGTKAVLDQEEIPNIAIDEVTGFPEMMD
CCCCEEEEECCCCHHHHHHHHHHHCCCEEECCCCCEEEECHHHCCCEEHHHHCCCHHHHC
GRVKTLHPLIHGALLGRRDLDSHMKSMAEHHISPIDLVVVNLYPFKETLLAGGSQAEMIE
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHCCCCHHHHHH
KIDIGGPSMLRSAAKNHAAVTVVCDPMDYEKVEKELSADGETSLELRQQLAAKVFRHTAS
HHCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
YDALIAQYLTEEFPVEDTKPEKLTLTYDLKQGMRYGENPQQSADFYESGLPTTYSIAQSH
HHHHHHHHHHHHCCCCCCCCCEEEEEEEHHHCCCCCCCCCHHHHHHHCCCCCEEHHHHHH
QLHGKELSYNNVRDADAALQIARDFDEPTVVALKHMNPCGIGTAKNIEQAWDYAYEADPV
CCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCH
SIFGGIIVLNREVTEETAQKMSKIFLEIIIAPSYSKEALEILSKKKNIRLLTVDFSKKEQ
HHCCCEEEEECHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHCCCCEEEEEEECCCCHH
SEKEALVTGVLGGLLVQNQDVIVENPKEWTVATKVQPTDRQMEAMKFAWKAVKFVKSNGI
HHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCE
IVTNDHQTLGVGPGQTNRVGSVKIALEATADKDALLQENAVLGSDAFFPFADNIDEIAKA
EEECCCCEEECCCCCCCCCCEEEEEEEECCCHHHHHHCCCEECCCCCCCCCCCHHHHHHH
GIKAIVQPGGSVRDQEVIEACDKYGIAMVFTGLRHFRH
HHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SKRALISVSDKEGIVEFAKKIQELGWEIISTGGTKAVLDQEEIPNIAIDEVTGFPEMMD
CCCEEEEECCCCHHHHHHHHHHHCCCEEECCCCCEEEECHHHCCCEEHHHHCCCHHHHC
GRVKTLHPLIHGALLGRRDLDSHMKSMAEHHISPIDLVVVNLYPFKETLLAGGSQAEMIE
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHCCCCHHHHHH
KIDIGGPSMLRSAAKNHAAVTVVCDPMDYEKVEKELSADGETSLELRQQLAAKVFRHTAS
HHCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
YDALIAQYLTEEFPVEDTKPEKLTLTYDLKQGMRYGENPQQSADFYESGLPTTYSIAQSH
HHHHHHHHHHHHCCCCCCCCCEEEEEEEHHHCCCCCCCCCHHHHHHHCCCCCEEHHHHHH
QLHGKELSYNNVRDADAALQIARDFDEPTVVALKHMNPCGIGTAKNIEQAWDYAYEADPV
CCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCH
SIFGGIIVLNREVTEETAQKMSKIFLEIIIAPSYSKEALEILSKKKNIRLLTVDFSKKEQ
HHCCCEEEEECHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHCCCCEEEEEEECCCCHH
SEKEALVTGVLGGLLVQNQDVIVENPKEWTVATKVQPTDRQMEAMKFAWKAVKFVKSNGI
HHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCE
IVTNDHQTLGVGPGQTNRVGSVKIALEATADKDALLQENAVLGSDAFFPFADNIDEIAKA
EEECCCCEEECCCCCCCCCCEEEEEEEECCCHHHHHHCCCEECCCCCCCCCCCHHHHHHH
GIKAIVQPGGSVRDQEVIEACDKYGIAMVFTGLRHFRH
HHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]