Click here to switch to the map view.

The map label for this gene is thyA [H]

Identifier: 125623803

GI number: 125623803

Start: 930251

End: 931090

Strand: Direct

Name: thyA [H]

Synonym: llmg_0964

Alternate gene names: 125623803

Gene position: 930251-931090 (Clockwise)

Preceding gene: 125623802

Following gene: 125623804

Centisome position: 36.78

GC content: 35.12

Gene sequence:

>840_bases
ATGACTTACGCAGATCAAGTTTTTAAACAAAATATCCAAAATATCCTAGATAATGGTGTTTTTTCAGAAAATGCAAGACC
AAAGTATAAGGATGGTCAAATGGCGAATAGCAAATATGTCACTGGTTCATTCGTTACTTATGATTTGCAAAAGGGGGAGT
TTCCAATTACCACTTTGCGTCCAATTCCAATCAAATCTGCTATTAAAGAATTGATGTGGATATACCAAGACCAAACAAGT
GAACTTTCTGTTCTCGAAGAGAAGTATGGAGTCAAATACTGGGGAGAATGGGGAATTGGTGATGGTACGATTGGGCAACG
TTATGGTGCAACAGTCAAAAAATATAATATCATTGGTAAATTATTAGAAGGCTTGGCCAAAAATCCATGGAATCGTCGTA
ATATCATCAACCTTTGGCAGTATGAAGATTTTGAGGAAACAGAAGGTCTTTTACCATGTGCTTTCCAAACGATGTTTGAT
GTCCGTCGAGAAAAAGATGGTCAGATTTATTTGGATGCCACACTGATTCAACGTTCAAACGATATGCTTGTAGCCCACCA
TATCAATGCGATGCAATATGTTGCTTTGCAAATGATGATTGCAAAACATTTTTCTTGGAAAGTTGGGAAATTCTTTTATT
TTGTAAATAATTTACATATTTATGATAATCAGTTTGAGCAGGCAAATGAATTAATGAAGCGAACAGCTTCTGAAAAAGAA
CCTCGTTTGGTCCTTAATGTTCCTGATGGTACAAACTTTTTCGATATTAAACCTGAAGATTTTGAACTTGTGGACTATGA
GCCAGTAAAACCTCAATTGAAATTTGATTTAGCAATTTAA

Upstream 100 bases:

>100_bases
CTATCCTGATAATTCTGAGAGGTTATTTTGGGAAATACTATTGAACCATATCGAGGTGGTGTGGTATAATGAAGGGAATT
AAAAAAGATAGGAAAATTTC

Downstream 100 bases:

>100_bases
ATTAATCTATAAGTTACTGACAAAACTGTCAGTAACTTTTTTTGTGGGAAAAATGTATTTTTATGACCGTAAAGAATCTG
TCAGTAGAAGTCTGAAATTC

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MTYADQVFKQNIQNILDNGVFSENARPKYKDGQMANSKYVTGSFVTYDLQKGEFPITTLRPIPIKSAIKELMWIYQDQTS
ELSVLEEKYGVKYWGEWGIGDGTIGQRYGATVKKYNIIGKLLEGLAKNPWNRRNIINLWQYEDFEETEGLLPCAFQTMFD
VRREKDGQIYLDATLIQRSNDMLVAHHINAMQYVALQMMIAKHFSWKVGKFFYFVNNLHIYDNQFEQANELMKRTASEKE
PRLVLNVPDGTNFFDIKPEDFELVDYEPVKPQLKFDLAI

Sequences:

>Translated_279_residues
MTYADQVFKQNIQNILDNGVFSENARPKYKDGQMANSKYVTGSFVTYDLQKGEFPITTLRPIPIKSAIKELMWIYQDQTS
ELSVLEEKYGVKYWGEWGIGDGTIGQRYGATVKKYNIIGKLLEGLAKNPWNRRNIINLWQYEDFEETEGLLPCAFQTMFD
VRREKDGQIYLDATLIQRSNDMLVAHHINAMQYVALQMMIAKHFSWKVGKFFYFVNNLHIYDNQFEQANELMKRTASEKE
PRLVLNVPDGTNFFDIKPEDFELVDYEPVKPQLKFDLAI
>Mature_278_residues
TYADQVFKQNIQNILDNGVFSENARPKYKDGQMANSKYVTGSFVTYDLQKGEFPITTLRPIPIKSAIKELMWIYQDQTSE
LSVLEEKYGVKYWGEWGIGDGTIGQRYGATVKKYNIIGKLLEGLAKNPWNRRNIINLWQYEDFEETEGLLPCAFQTMFDV
RREKDGQIYLDATLIQRSNDMLVAHHINAMQYVALQMMIAKHFSWKVGKFFYFVNNLHIYDNQFEQANELMKRTASEKEP
RLVLNVPDGTNFFDIKPEDFELVDYEPVKPQLKFDLAI

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=303, Percent_Identity=27.0627062706271, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI1789191, Length=276, Percent_Identity=27.536231884058, Blast_Score=94, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324648, Length=195, Percent_Identity=28.2051282051282, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17137556, Length=268, Percent_Identity=27.2388059701493, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 32578; Mature: 32446

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYADQVFKQNIQNILDNGVFSENARPKYKDGQMANSKYVTGSFVTYDLQKGEFPITTLR
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECEEEEEEECCCCCCEEECC
PIPIKSAIKELMWIYQDQTSELSVLEEKYGVKYWGEWGIGDGTIGQRYGATVKKYNIIGK
CCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCCCCCCCCHHHCCHHHHHHHHHH
LLEGLAKNPWNRRNIINLWQYEDFEETEGLLPCAFQTMFDVRREKDGQIYLDATLIQRSN
HHHHHHCCCCCCCCCEEEECCCCHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEEEEECCC
DMLVAHHINAMQYVALQMMIAKHFSWKVGKFFYFVNNLHIYDNQFEQANELMKRTASEKE
CEEEEEHHHHHHHHHHHHHHHHHCCCHHHEEEEEEEEEEEECCHHHHHHHHHHHHCCCCC
PRLVLNVPDGTNFFDIKPEDFELVDYEPVKPQLKFDLAI
CEEEEECCCCCCEEECCCCCCEEECCCCCCCCEEEEECC
>Mature Secondary Structure 
TYADQVFKQNIQNILDNGVFSENARPKYKDGQMANSKYVTGSFVTYDLQKGEFPITTLR
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECEEEEEEECCCCCCEEECC
PIPIKSAIKELMWIYQDQTSELSVLEEKYGVKYWGEWGIGDGTIGQRYGATVKKYNIIGK
CCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCCCCCCCCHHHCCHHHHHHHHHH
LLEGLAKNPWNRRNIINLWQYEDFEETEGLLPCAFQTMFDVRREKDGQIYLDATLIQRSN
HHHHHHCCCCCCCCCEEEECCCCHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEEEEECCC
DMLVAHHINAMQYVALQMMIAKHFSWKVGKFFYFVNNLHIYDNQFEQANELMKRTASEKE
CEEEEEHHHHHHHHHHHHHHHHHCCCHHHEEEEEEEEEEEECCHHHHHHHHHHHHCCCCC
PRLVLNVPDGTNFFDIKPEDFELVDYEPVKPQLKFDLAI
CEEEEECCCCCCEEECCCCCCEEECCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2117882; 11337471 [H]