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The map label for this gene is trmD [H]

Identifier: 125623778

GI number: 125623778

Start: 905761

End: 906516

Strand: Direct

Name: trmD [H]

Synonym: llmg_0937

Alternate gene names: 125623778

Gene position: 905761-906516 (Clockwise)

Preceding gene: 125623777

Following gene: 125623779

Centisome position: 35.81

GC content: 38.76

Gene sequence:

>756_bases
ATGACTAAGGGCATGAGAATTGATATTTTAAGTATTTTTCCTGATATGTTTGGTCCACTTAACCAGTCAATAGTTGGCAA
GGCTCAAGATAAAGGTATTTTAGAATTACATACTCATGATTTTCGCGAAAATGCCACAAATAAACAGCGACATGTCGATG
ATATGCCTTATGGTGGGGGACAAGGGATGTTGTTGATGCCTCAACCTATCTTTGATACGATGGATAAAATTCCACAAAAA
TCTGAAAAGCCTGCACGGGTGATTCTTTTAGATCCAGCCGGAAAAAAATTTGATCAAAAAATGGCTGAGGAATTGTCAGA
AGAAGAACAACTAATCTTTATTTGTGGACACTACGAAGGCTATGATGAACGAATTAAAACTTTGGTGACAGATGAGGTAT
CTTTGGGAGACTTTGTCTTAACAGGAGGAGAAGTGGCAGCAACTGTAATGATTGATGCTGTTGTTCGTCTTATACCTGGC
GTTTTGGGGAAAGCGGCTAGTCATGAAGATGATTCATTTTCTAGTGGGCTTTTAGAATATCCCCAATACACTCGCCCAGA
AGATTTTAGAGGAATGAAGGTTCCCGAAGTCTTGATGAGCGGTCATCATGAGAATATTCGCAAGTGGCGTTTGAGAGAAT
CTTTGAAAAAAACACTAGAGCGCCGTCCAGATTTATTGGATACATACGAACCTAATGACGAAGAACTAAAAATGCTACAA
TTATTGCGTGAAAATGTTCAAGATGTGGTAAAATGA

Upstream 100 bases:

>100_bases
AAAGGAAAACGTGATTTACTTTTACCTTATATCCCACCTGTTGTCCTGAAAGTAGATGTGGCTCAGCATCGAGTTGATGT
GGACATCATGGAAGGATTGG

Downstream 100 bases:

>100_bases
AGTAGTGTTAAGAATTTTAGTGAATATTGGGGATGCCTATTGAATTAGGATAATGAAATGTGTGTTCTTATTGTTCATGA
ATTGGAATATGACTAAATTT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MTKGMRIDILSIFPDMFGPLNQSIVGKAQDKGILELHTHDFRENATNKQRHVDDMPYGGGQGMLLMPQPIFDTMDKIPQK
SEKPARVILLDPAGKKFDQKMAEELSEEEQLIFICGHYEGYDERIKTLVTDEVSLGDFVLTGGEVAATVMIDAVVRLIPG
VLGKAASHEDDSFSSGLLEYPQYTRPEDFRGMKVPEVLMSGHHENIRKWRLRESLKKTLERRPDLLDTYEPNDEELKMLQ
LLRENVQDVVK

Sequences:

>Translated_251_residues
MTKGMRIDILSIFPDMFGPLNQSIVGKAQDKGILELHTHDFRENATNKQRHVDDMPYGGGQGMLLMPQPIFDTMDKIPQK
SEKPARVILLDPAGKKFDQKMAEELSEEEQLIFICGHYEGYDERIKTLVTDEVSLGDFVLTGGEVAATVMIDAVVRLIPG
VLGKAASHEDDSFSSGLLEYPQYTRPEDFRGMKVPEVLMSGHHENIRKWRLRESLKKTLERRPDLLDTYEPNDEELKMLQ
LLRENVQDVVK
>Mature_250_residues
TKGMRIDILSIFPDMFGPLNQSIVGKAQDKGILELHTHDFRENATNKQRHVDDMPYGGGQGMLLMPQPIFDTMDKIPQKS
EKPARVILLDPAGKKFDQKMAEELSEEEQLIFICGHYEGYDERIKTLVTDEVSLGDFVLTGGEVAATVMIDAVVRLIPGV
LGKAASHEDDSFSSGLLEYPQYTRPEDFRGMKVPEVLMSGHHENIRKWRLRESLKKTLERRPDLLDTYEPNDEELKMLQL
LRENVQDVVK

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=237, Percent_Identity=46.4135021097046, Blast_Score=220, Evalue=6e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 28570; Mature: 28439

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKGMRIDILSIFPDMFGPLNQSIVGKAQDKGILELHTHDFRENATNKQRHVDDMPYGGG
CCCCCEEHHHHHHHHHHCCCCHHHHCCCCCCCEEEEECHHHHHCCCCHHHCCCCCCCCCC
QGMLLMPQPIFDTMDKIPQKSEKPARVILLDPAGKKFDQKMAEELSEEEQLIFICGHYEG
CCEEECCCHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCC
YDERIKTLVTDEVSLGDFVLTGGEVAATVMIDAVVRLIPGVLGKAASHEDDSFSSGLLEY
HHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHCC
PQYTRPEDFRGMKVPEVLMSGHHENIRKWRLRESLKKTLERRPDLLDTYEPNDEELKMLQ
CCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
LLRENVQDVVK
HHHHHHHHHHC
>Mature Secondary Structure 
TKGMRIDILSIFPDMFGPLNQSIVGKAQDKGILELHTHDFRENATNKQRHVDDMPYGGG
CCCCEEHHHHHHHHHHCCCCHHHHCCCCCCCEEEEECHHHHHCCCCHHHCCCCCCCCCC
QGMLLMPQPIFDTMDKIPQKSEKPARVILLDPAGKKFDQKMAEELSEEEQLIFICGHYEG
CCEEECCCHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCC
YDERIKTLVTDEVSLGDFVLTGGEVAATVMIDAVVRLIPGVLGKAASHEDDSFSSGLLEY
HHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHCC
PQYTRPEDFRGMKVPEVLMSGHHENIRKWRLRESLKKTLERRPDLLDTYEPNDEELKMLQ
CCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
LLRENVQDVVK
HHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]