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The map label for this gene is ptnD [H]

Identifier: 125623582

GI number: 125623582

Start: 709596

End: 710519

Strand: Reverse

Name: ptnD [H]

Synonym: llmg_0727

Alternate gene names: 125623582

Gene position: 710519-709596 (Counterclockwise)

Preceding gene: 125623583

Following gene: 125623581

Centisome position: 28.09

GC content: 41.88

Gene sequence:

>924_bases
ATGTCTGATAAAAAAGTAACTCTTGATAAGAAAATCCGTCGTAGCGTTATGTGGCGTTCAATGTTCCTCCAAGGTTCTTG
GAACTACGAACGTATGCAAAATGGTGGTTGGGCTTACTCGCTCATTCCAGCATTGAAAAAACTCTACCCTTCTGGCGAAG
AAGCTAAAGAAGCTTTGAAACGTCACTTGGAATTCTTTAATACTCACCCATACGTTGCTGCTCCTATCATCGGTGTAACT
CTTGCCCTTGAAGAAGAACGTGCTAACGGTGCTGATATCGATGATGCCGCTATTCAAGGGGTAAAAGTTGGTATGATGGG
TCCTCTTGCCGGTATCGGTGACCCAGTCTTCTGGTTTACAGTTCGCCCTATCGTTGGTGCTATCGCAGCTTCATTGGCTG
TTGGTGGTTCAATCATCGCTCCACTCTTCTTCTTCATCGTGTGGAATGCAATCCGTATCGCTTTCTTGTGGTACACTCAA
GAATTTGGTTATAAATCAGGTTCTGCAATCACTAAAGACCTTGGTGGAGGACTTCTCCAAACTGTTACTAAAGGTGCATC
TATCCTTGGTATGTTCGTCCTTGGTGTATTGATTCAACGTTGGGTAACAACCAACTTCAACGGTGCTAACGCTGTTGTTT
CAAAAATTCCTTTACAAAAAGGTGCTTACGTAGAATTCCCTAAAGGTTCTGTATCTGGTACACAACTTCATGAGATTCTT
GGTCAAGTAGGTAACAAACTTTCTCTTGATCCTACAAAAGTAACTTATCTTCAAGATAACTTGAATCAATTGATTCCTGG
TCTTGCTGGTTTGCTTATCACATTCCTTTGTATGTGGTTGCTTAAGAAAAAAGTTTCTCCAATCGTTATTATCTTTGGTC
TCTTCATCGTGGGTATCCTCGGTCGTTGGGCTCAAATCATGTAA

Upstream 100 bases:

>100_bases
CCTTCAATCTTCTGGTGGTTCTGGAAATGGTACTGCATCATCATCAGGTGACCCAATTGGCGACATCTTGAACGACTACT
AAGAAAGGAGGATCTAAAAA

Downstream 100 bases:

>100_bases
TCAATGGATTAACAAAAACCAATCTTCGGATTGGTTTTTTTATTATAATTTATCACTTGAACTTTAGAATATTTAGAGCA
AGTAAAGCGTTTTTATGGTA

Product: mannose-specific PTS system component IID

Products: protein histidine; sugar phosphate; D-glucosamine-6-phosphate [Cytoplasm]; pyruvate; glucose-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EII-M-Man; EIID-Man; PTS system mannose-specific EIID component [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSDKKVTLDKKIRRSVMWRSMFLQGSWNYERMQNGGWAYSLIPALKKLYPSGEEAKEALKRHLEFFNTHPYVAAPIIGVT
LALEEERANGADIDDAAIQGVKVGMMGPLAGIGDPVFWFTVRPIVGAIAASLAVGGSIIAPLFFFIVWNAIRIAFLWYTQ
EFGYKSGSAITKDLGGGLLQTVTKGASILGMFVLGVLIQRWVTTNFNGANAVVSKIPLQKGAYVEFPKGSVSGTQLHEIL
GQVGNKLSLDPTKVTYLQDNLNQLIPGLAGLLITFLCMWLLKKKVSPIVIIFGLFIVGILGRWAQIM

Sequences:

>Translated_307_residues
MSDKKVTLDKKIRRSVMWRSMFLQGSWNYERMQNGGWAYSLIPALKKLYPSGEEAKEALKRHLEFFNTHPYVAAPIIGVT
LALEEERANGADIDDAAIQGVKVGMMGPLAGIGDPVFWFTVRPIVGAIAASLAVGGSIIAPLFFFIVWNAIRIAFLWYTQ
EFGYKSGSAITKDLGGGLLQTVTKGASILGMFVLGVLIQRWVTTNFNGANAVVSKIPLQKGAYVEFPKGSVSGTQLHEIL
GQVGNKLSLDPTKVTYLQDNLNQLIPGLAGLLITFLCMWLLKKKVSPIVIIFGLFIVGILGRWAQIM
>Mature_306_residues
SDKKVTLDKKIRRSVMWRSMFLQGSWNYERMQNGGWAYSLIPALKKLYPSGEEAKEALKRHLEFFNTHPYVAAPIIGVTL
ALEEERANGADIDDAAIQGVKVGMMGPLAGIGDPVFWFTVRPIVGAIAASLAVGGSIIAPLFFFIVWNAIRIAFLWYTQE
FGYKSGSAITKDLGGGLLQTVTKGASILGMFVLGVLIQRWVTTNFNGANAVVSKIPLQKGAYVEFPKGSVSGTQLHEILG
QVGNKLSLDPTKVTYLQDNLNQLIPGLAGLLITFLCMWLLKKKVSPIVIIFGLFIVGILGRWAQIM

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3716

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IID

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIID domain [H]

Homologues:

Organism=Escherichia coli, GI1788122, Length=306, Percent_Identity=49.3464052287582, Blast_Score=298, Evalue=3e-82,
Organism=Escherichia coli, GI1789529, Length=297, Percent_Identity=31.3131313131313, Blast_Score=133, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004704
- InterPro:   IPR018405 [H]

Pfam domain/function: PF03613 EIID-AGA [H]

EC number: NA

Molecular weight: Translated: 33621; Mature: 33490

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: PS51108 PTS_EIID

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKKVTLDKKIRRSVMWRSMFLQGSWNYERMQNGGWAYSLIPALKKLYPSGEEAKEALK
CCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHH
RHLEFFNTHPYVAAPIIGVTLALEEERANGADIDDAAIQGVKVGMMGPLAGIGDPVFWFT
HHHHHHCCCCCHHHHHHHHHEEEECCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHH
VRPIVGAIAASLAVGGSIIAPLFFFIVWNAIRIAFLWYTQEFGYKSGSAITKDLGGGLLQ
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHH
TVTKGASILGMFVLGVLIQRWVTTNFNGANAVVSKIPLQKGAYVEFPKGSVSGTQLHEIL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHH
GQVGNKLSLDPTKVTYLQDNLNQLIPGLAGLLITFLCMWLLKKKVSPIVIIFGLFIVGIL
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
GRWAQIM
HHHHHCC
>Mature Secondary Structure 
SDKKVTLDKKIRRSVMWRSMFLQGSWNYERMQNGGWAYSLIPALKKLYPSGEEAKEALK
CCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHH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HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N p -phosphohistidine; sugar; phosphoenolpyruvate; glucosamine [Periplasm]; phosphoenolpyruvate; beta-D-glucose [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; fructose [Periplasm] [C]

Specific reaction: protein N p -phosphohistidine + sugar = protein histidine + sugar phosphate phosphoenolpyruvate + glucosamine [Periplasm] = D-glucosamine-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm]

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]