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The map label for this gene is icaA [H]

Identifier: 125623506

GI number: 125623506

Start: 641521

End: 642735

Strand: Direct

Name: icaA [H]

Synonym: llmg_0646

Alternate gene names: 125623506

Gene position: 641521-642735 (Clockwise)

Preceding gene: 125623505

Following gene: 125623509

Centisome position: 25.36

GC content: 33.42

Gene sequence:

>1215_bases
ATGATTATTTCAAAAATGGTAGATATTTATGCTGTTTATTATCCAATTGTTTTATCATTTATTTGGGCAAGTGGTACTTT
CTTATCTCGCTGGAAAGATAAAAGAAGAGCTAGGGGATTAAATGATAGAGAGAAAATTAGTATTGTCATTTCTGCATATA
ATGAAGAAGAAACGATAGAAGAAGTATTATTATCTTTGAGAAATTTAAATTATCCTGCACTTGAAATTTTCGTTGTTGAT
GTTAAAAGCAGTGACCGAACATTGCAAAAACTTCATGCGTTCAAAAGGCGTTTCAATAATTGGGAAGCTTTAACAATTTT
AGAGCAAAAAGAAAATAAAGGGAAAGCGACGGCCCTAAATGTTGCTTTGAATCAGGTTACGAGTAAATATATGCTTGTTA
TTGATGCAGATTCTTATCTTTCTGCGGATGCTTTAGATTATTTATTAGCAGAATTAGTTTCTGATTCGCGTAATGGTGCG
GTTACAGGTCGTCCAATTGTGCGAAATAGAACCACTTTACTTGGTAAACTACAGACCGTAGAATATTTGAGTGTAATTGA
TGCAATTAAGCGAGCACAAAGTCTATTTTTAGGTGCTATTATGACTGTATCTGGCGTTATTGTCATGTATCGTGTTGAAG
CTTTGAAAGAAATAGGTGGTTTTGATACTGAGGTAATGACAGAGGACATTGATGTAACTTGGCGATTACATAAGCAAAAT
TGGAAGGTAAAATATGTTCCCAAAGCCTTGAGCTATATCCTTGTGCCAGAAAATTTAAAATCACTTGTGAAACAACGCCG
AAGATGGGCAGTTGGAGGAGTAGAAGTTTTGATTTCAAATTTTTCTTGGGTCTTTAAAAGAGGGGATTTTAAACATCGAT
TTCTTTTATTTGAAATGATTTGTAGTCATATTTGGAGTTGGTTCTTTCTTATAGAGTCTTACCAATACTTTTTACAGATG
CTATTAAATCAAGAATTTAAAGTATCGGGACAGATTATAGTAATTTTTGTTTTGATTTCGTTTGCTGTTTTCTTTTTGGG
TTTGAACAACGATAGAGGTGAGAGTAAATTGTCTTTATCTGACAAAATGATTTTACCAGTCTATCTTGTTGCTTATTGGA
TTTTGAATCTTGTCAGCGCAATCTCGGCTGAAATTGTTGTCTTAAGTAATCGGACTAACAAAGGAAAATGGGAAAGTCCA
GATAGGGGAATATAA

Upstream 100 bases:

>100_bases
ATACCAGTCGCTTTTAGCAGCGCAGCTTTGGTAGGAATATTAGTCGGGAAAATTTTAGGCCTTCCTTTTAAAAAGAAGGA
AAATAGAGGAAGTTAAAATT

Downstream 100 bases:

>100_bases
AATGGAATTAGTTATACGTAAGAAAAAGAAAAAAAGACATTTTTTTGAATTTATGGCTGTCTTCATGTGTTGGGTCTTTA
TTTTAAGTATTTTTATATTC

Product: intercellular adhesion protein A

Products: NA

Alternate protein names: PNAG synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm polysaccharide intercellular adhesin synthesis protein IcaA; Biofilm PIA synthesis protein IcaA; Intercellular adhesion protein A; N-acetylglucosaminyltransferase IcaA [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MIISKMVDIYAVYYPIVLSFIWASGTFLSRWKDKRRARGLNDREKISIVISAYNEEETIEEVLLSLRNLNYPALEIFVVD
VKSSDRTLQKLHAFKRRFNNWEALTILEQKENKGKATALNVALNQVTSKYMLVIDADSYLSADALDYLLAELVSDSRNGA
VTGRPIVRNRTTLLGKLQTVEYLSVIDAIKRAQSLFLGAIMTVSGVIVMYRVEALKEIGGFDTEVMTEDIDVTWRLHKQN
WKVKYVPKALSYILVPENLKSLVKQRRRWAVGGVEVLISNFSWVFKRGDFKHRFLLFEMICSHIWSWFFLIESYQYFLQM
LLNQEFKVSGQIIVIFVLISFAVFFLGLNNDRGESKLSLSDKMILPVYLVAYWILNLVSAISAEIVVLSNRTNKGKWESP
DRGI

Sequences:

>Translated_404_residues
MIISKMVDIYAVYYPIVLSFIWASGTFLSRWKDKRRARGLNDREKISIVISAYNEEETIEEVLLSLRNLNYPALEIFVVD
VKSSDRTLQKLHAFKRRFNNWEALTILEQKENKGKATALNVALNQVTSKYMLVIDADSYLSADALDYLLAELVSDSRNGA
VTGRPIVRNRTTLLGKLQTVEYLSVIDAIKRAQSLFLGAIMTVSGVIVMYRVEALKEIGGFDTEVMTEDIDVTWRLHKQN
WKVKYVPKALSYILVPENLKSLVKQRRRWAVGGVEVLISNFSWVFKRGDFKHRFLLFEMICSHIWSWFFLIESYQYFLQM
LLNQEFKVSGQIIVIFVLISFAVFFLGLNNDRGESKLSLSDKMILPVYLVAYWILNLVSAISAEIVVLSNRTNKGKWESP
DRGI
>Mature_404_residues
MIISKMVDIYAVYYPIVLSFIWASGTFLSRWKDKRRARGLNDREKISIVISAYNEEETIEEVLLSLRNLNYPALEIFVVD
VKSSDRTLQKLHAFKRRFNNWEALTILEQKENKGKATALNVALNQVTSKYMLVIDADSYLSADALDYLLAELVSDSRNGA
VTGRPIVRNRTTLLGKLQTVEYLSVIDAIKRAQSLFLGAIMTVSGVIVMYRVEALKEIGGFDTEVMTEDIDVTWRLHKQN
WKVKYVPKALSYILVPENLKSLVKQRRRWAVGGVEVLISNFSWVFKRGDFKHRFLLFEMICSHIWSWFFLIESYQYFLQM
LLNQEFKVSGQIIVIFVLISFAVFFLGLNNDRGESKLSLSDKMILPVYLVAYWILNLVSAISAEIVVLSNRTNKGKWESP
DRGI

Specific function: N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N-acetylglucosamine to produce the linear homopolymer poly-beta-1,6-N-acetyl-D- glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide.

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787259, Length=413, Percent_Identity=34.1404358353511, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI1786560, Length=230, Percent_Identity=27.3913043478261, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 46544; Mature: 46544

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIISKMVDIYAVYYPIVLSFIWASGTFLSRWKDKRRARGLNDREKISIVISAYNEEETIE
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHH
EVLLSLRNLNYPALEIFVVDVKSSDRTLQKLHAFKRRFNNWEALTILEQKENKGKATALN
HHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHH
VALNQVTSKYMLVIDADSYLSADALDYLLAELVSDSRNGAVTGRPIVRNRTTLLGKLQTV
HHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHH
EYLSVIDAIKRAQSLFLGAIMTVSGVIVMYRVEALKEIGGFDTEVMTEDIDVTWRLHKQN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEECCCCEEEEEECCC
WKVKYVPKALSYILVPENLKSLVKQRRRWAVGGVEVLISNFSWVFKRGDFKHRFLLFEMI
CEEEEECHHHHEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHH
CSHIWSWFFLIESYQYFLQMLLNQEFKVSGQIIVIFVLISFAVFFLGLNNDRGESKLSLS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEECCC
DKMILPVYLVAYWILNLVSAISAEIVVLSNRTNKGKWESPDRGI
CCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MIISKMVDIYAVYYPIVLSFIWASGTFLSRWKDKRRARGLNDREKISIVISAYNEEETIE
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHH
EVLLSLRNLNYPALEIFVVDVKSSDRTLQKLHAFKRRFNNWEALTILEQKENKGKATALN
HHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHH
VALNQVTSKYMLVIDADSYLSADALDYLLAELVSDSRNGAVTGRPIVRNRTTLLGKLQTV
HHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHH
EYLSVIDAIKRAQSLFLGAIMTVSGVIVMYRVEALKEIGGFDTEVMTEDIDVTWRLHKQN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEECCCCEEEEEECCC
WKVKYVPKALSYILVPENLKSLVKQRRRWAVGGVEVLISNFSWVFKRGDFKHRFLLFEMI
CEEEEECHHHHEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHH
CSHIWSWFFLIESYQYFLQMLLNQEFKVSGQIIVIFVLISFAVFFLGLNNDRGESKLSLS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEECCC
DKMILPVYLVAYWILNLVSAISAEIVVLSNRTNKGKWESPDRGI
CCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10496925 [H]