Click here to switch to the map view.

The map label for this gene is icaB [H]

Identifier: 125623509

GI number: 125623509

Start: 644291

End: 645115

Strand: Direct

Name: icaB [H]

Synonym: llmg_0649

Alternate gene names: 125623509

Gene position: 644291-645115 (Clockwise)

Preceding gene: 125623506

Following gene: 125623517

Centisome position: 25.47

GC content: 32.0

Gene sequence:

>825_bases
GTGACTCTAAGATTTAAGGATAACATCAAATATGTGAAAGAGAGCAAAAAAGAACAACCTGATTTCGCTTATGAGAGTTT
ACTTCAGGATAATGATGGAATTATTGTCCTTTGTTATCATCGTGTTCTCAAAGACACACCTATCACAAGCTTTGCTTCAA
CAATTTCTCAAAATTCGCAGTTACATGAATACAATGTTTTTTTAGAGGATTTTGAAAAACAAATGGAATTTTTGAAAGAA
AATCACATCTCGGTTTTAACGTCTGAACAACTCATCTCTAAAATTAATAAAAAAGAGGTAATAGGCAAAGCGGTTGTCAT
TACTTTTGATGATATCGATAAATCTCTGCCAGAAAATGCTTATCCAGTAATGAAAGAATTTGACTTACCTTTTACCCAAT
TTATTATTACCGGAAAAGTTGGTCAAACGATTGATGGTTCACAAATGTCTTCGTGGAAAGAAATAAAGGAGATGGATGAA
AATCCTTTGGTGATATCTGGTTTGCACACAAACGACTTACATTATCAGGAAAATCTTGAACCAGTTTTATCAACAAATAT
TTCGAAAAAAGTAGTCCAAAAAGATTATTTAAAAAGTCAAAAAACTTTTCAAGAAGAATTAAATAAAAAGGGGAATGTTT
TTGCTTATCCTTATGGGGCTCAAAATAAAGATTTAGAAAATTATATGCTCAAAGACGGAATCAGTGGAATATTTACGCTT
TCCCCAGGAGTGGTTACTAATGAAACTAAGTATAGTAGTATCCCTAGACTGATTGTTACGAAGGAAAATTGGAAAACGAT
TGAGCATTGGCTTGTAAAGAAATGA

Upstream 100 bases:

>100_bases
TGACGGATTGACCAGAATGATAGAAGGCGATAAGGGTTTCTTTAAATTCTTTTGAGTAGCGTTTTTGCATGTTTTTGTCC
TTTGTCTAAATTATACAATA

Downstream 100 bases:

>100_bases
AAGATACATCACTAAATATAAAATAAATTCTTAGAAGTGCGGTCTTTAAAAATAAATAAAAGAGCTCCTAAATATTAGAA
GCTCTTTTATTTATTTGTGA

Product: intercellular adhesion protein icaB

Products: NA

Alternate protein names: PNAG N-deacetylase; Poly-beta-1,6-GlcNAc N-deacetylase; Biofilm polysaccharide intercellular adhesin deacetylase; Biofilm PIA deacetylase; Intercellular adhesion protein B [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MTLRFKDNIKYVKESKKEQPDFAYESLLQDNDGIIVLCYHRVLKDTPITSFASTISQNSQLHEYNVFLEDFEKQMEFLKE
NHISVLTSEQLISKINKKEVIGKAVVITFDDIDKSLPENAYPVMKEFDLPFTQFIITGKVGQTIDGSQMSSWKEIKEMDE
NPLVISGLHTNDLHYQENLEPVLSTNISKKVVQKDYLKSQKTFQEELNKKGNVFAYPYGAQNKDLENYMLKDGISGIFTL
SPGVVTNETKYSSIPRLIVTKENWKTIEHWLVKK

Sequences:

>Translated_274_residues
MTLRFKDNIKYVKESKKEQPDFAYESLLQDNDGIIVLCYHRVLKDTPITSFASTISQNSQLHEYNVFLEDFEKQMEFLKE
NHISVLTSEQLISKINKKEVIGKAVVITFDDIDKSLPENAYPVMKEFDLPFTQFIITGKVGQTIDGSQMSSWKEIKEMDE
NPLVISGLHTNDLHYQENLEPVLSTNISKKVVQKDYLKSQKTFQEELNKKGNVFAYPYGAQNKDLENYMLKDGISGIFTL
SPGVVTNETKYSSIPRLIVTKENWKTIEHWLVKK
>Mature_273_residues
TLRFKDNIKYVKESKKEQPDFAYESLLQDNDGIIVLCYHRVLKDTPITSFASTISQNSQLHEYNVFLEDFEKQMEFLKEN
HISVLTSEQLISKINKKEVIGKAVVITFDDIDKSLPENAYPVMKEFDLPFTQFIITGKVGQTIDGSQMSSWKEIKEMDEN
PLVISGLHTNDLHYQENLEPVLSTNISKKVVQKDYLKSQKTFQEELNKKGNVFAYPYGAQNKDLENYMLKDGISGIFTLS
PGVVTNETKYSSIPRLIVTKENWKTIEHWLVKK

Specific function: Catalyzes the N-deacetylation of poly-beta-1,6-N-acetyl- D-glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide. In fact, the IcaB deacetylase converts 15 to 20% of the GlcNAc residues of PNAG to glucosamine. N-deacetylation is cr

COG id: COG0726

COG function: function code G; Predicted xylanase/chitin deacetylase

Gene ontology:

Cell location: Secreted, cell wall. Note=Attached to the cell surface [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide deacetylase family [H]

Homologues:

Organism=Escherichia coli, GI1786322, Length=228, Percent_Identity=26.7543859649123, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011330
- InterPro:   IPR002509 [H]

Pfam domain/function: PF01522 Polysacc_deac_1 [H]

EC number: NA

Molecular weight: Translated: 31646; Mature: 31515

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLRFKDNIKYVKESKKEQPDFAYESLLQDNDGIIVLCYHRVLKDTPITSFASTISQNSQ
CEEEECCCHHHHHHCCCCCCCHHHHHHHCCCCCEEEEEEHHHHCCCCHHHHHHHHHCCCC
LHEYNVFLEDFEKQMEFLKENHISVLTSEQLISKINKKEVIGKAVVITFDDIDKSLPENA
HHHHHHHHHHHHHHHHHHHHCCHHEEEHHHHHHHHHHHHHCCEEEEEEECHHHHHCCCCC
YPVMKEFDLPFTQFIITGKVGQTIDGSQMSSWKEIKEMDENPLVISGLHTNDLHYQENLE
CCHHHHCCCCHHHEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHCCC
PVLSTNISKKVVQKDYLKSQKTFQEELNKKGNVFAYPYGAQNKDLENYMLKDGISGIFTL
HHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHCCCEEEEE
SPGVVTNETKYSSIPRLIVTKENWKTIEHWLVKK
CCCCEECCCHHCCCCEEEEECCCHHHHHHHHCCC
>Mature Secondary Structure 
TLRFKDNIKYVKESKKEQPDFAYESLLQDNDGIIVLCYHRVLKDTPITSFASTISQNSQ
EEEECCCHHHHHHCCCCCCCHHHHHHHCCCCCEEEEEEHHHHCCCCHHHHHHHHHCCCC
LHEYNVFLEDFEKQMEFLKENHISVLTSEQLISKINKKEVIGKAVVITFDDIDKSLPENA
HHHHHHHHHHHHHHHHHHHHCCHHEEEHHHHHHHHHHHHHCCEEEEEEECHHHHHCCCCC
YPVMKEFDLPFTQFIITGKVGQTIDGSQMSSWKEIKEMDENPLVISGLHTNDLHYQENLE
CCHHHHCCCCHHHEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHCCC
PVLSTNISKKVVQKDYLKSQKTFQEELNKKGNVFAYPYGAQNKDLENYMLKDGISGIFTL
HHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHCCCEEEEE
SPGVVTNETKYSSIPRLIVTKENWKTIEHWLVKK
CCCCEECCCHHCCCCEEEEECCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA