Click here to switch to the map view.

The map label for this gene is hemK [H]

Identifier: 125623425

GI number: 125623425

Start: 553288

End: 554100

Strand: Direct

Name: hemK [H]

Synonym: llmg_0560

Alternate gene names: 125623425

Gene position: 553288-554100 (Clockwise)

Preceding gene: 125623424

Following gene: 125623426

Centisome position: 21.87

GC content: 34.69

Gene sequence:

>813_bases
ATGCTTTGGATTGAAGCTGTGCGAACTTTGTCAGCAGATTTAGAAGAACCTTTTGAGTTAGAGTTTATCTGGCGAAATTT
ACATGAACTTAATAAGTTATCTTGGTTGAATTTAATGCGTGAAAAAATTACTGACCAAGAGTTAAATTTACTGACAGAGC
TATCAGAACGTTTGATTAAAAACGAACCCCCACAATATATTGTGGGCTGGGCAGAATTTTATGAATTAAAATTTAAGGTT
GATGAGCGAGTGCTCATTCCACGTCCAGAAACTGAAGAATTAGTTGAAATGATTTTAACTGAAAATAACAATGATTCGCT
GAAAATTTTAGATATTGGAACGGGCTCCGGAGCAATTGCAATTTCTTTAGCTAAAGCTCGGCAAAATTGGTCAGTAAAAG
CTTCTGACATTTCACAAAATGCGCTAGAATTGGCGGCTGAAAATGCGAAAATGAATCATGTCAATTTGGAATTTATTCAA
TCAGATGTGATGGATGAACTTACTGACAGGTTTGATATCATAGTTTCCAATCCCCCCTATATTGCTTTTGATGAAACTTA
TGAAATGGATGATTCTGTCATTAAATATGAGCCTGATTTAGCCCTTTTTGCCAAAAATCAAGGTTTAGCAATTTACCAAA
AAATTGCTGACCAAGCGGTCAACCATCTGACAGATGATGGAAAAATTTATTTGGAAATTGGCTATAAGCAAGGAAAAGCT
GTTCAAGCTATTTTTCAAGAAAAATTTACTGACAGGCTTGTTAGCATTCACCAGGATATATTTGGAAAAGATAGAATGAT
TAGTGTCAAATGA

Upstream 100 bases:

>100_bases
TTGATAAAGCCTGATTTTATTGGGCTTTTTTGCTATACTTGAAAGAGTAATTTAAAAGAGATTAGAAAAAATAAAAAGTT
TTAGAAAGTGGAAAAGTATA

Downstream 100 bases:

>100_bases
TAAAAGCTACTGTTGGTATATTTAAGGAGAAAAATGAATTCTTTAATTTTTGAACAAGTATTTCAGCAAAAAAAAGATTT
TTTACCAATATTATTATTAG

Product: putative protoporphyrinogen oxidase

Products: NA

Alternate protein names: HemK protein homolog; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MLWIEAVRTLSADLEEPFELEFIWRNLHELNKLSWLNLMREKITDQELNLLTELSERLIKNEPPQYIVGWAEFYELKFKV
DERVLIPRPETEELVEMILTENNNDSLKILDIGTGSGAIAISLAKARQNWSVKASDISQNALELAAENAKMNHVNLEFIQ
SDVMDELTDRFDIIVSNPPYIAFDETYEMDDSVIKYEPDLALFAKNQGLAIYQKIADQAVNHLTDDGKIYLEIGYKQGKA
VQAIFQEKFTDRLVSIHQDIFGKDRMISVK

Sequences:

>Translated_270_residues
MLWIEAVRTLSADLEEPFELEFIWRNLHELNKLSWLNLMREKITDQELNLLTELSERLIKNEPPQYIVGWAEFYELKFKV
DERVLIPRPETEELVEMILTENNNDSLKILDIGTGSGAIAISLAKARQNWSVKASDISQNALELAAENAKMNHVNLEFIQ
SDVMDELTDRFDIIVSNPPYIAFDETYEMDDSVIKYEPDLALFAKNQGLAIYQKIADQAVNHLTDDGKIYLEIGYKQGKA
VQAIFQEKFTDRLVSIHQDIFGKDRMISVK
>Mature_270_residues
MLWIEAVRTLSADLEEPFELEFIWRNLHELNKLSWLNLMREKITDQELNLLTELSERLIKNEPPQYIVGWAEFYELKFKV
DERVLIPRPETEELVEMILTENNNDSLKILDIGTGSGAIAISLAKARQNWSVKASDISQNALELAAENAKMNHVNLEFIQ
SDVMDELTDRFDIIVSNPPYIAFDETYEMDDSVIKYEPDLALFAKNQGLAIYQKIADQAVNHLTDDGKIYLEIGYKQGKA
VQAIFQEKFTDRLVSIHQDIFGKDRMISVK

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=251, Percent_Identity=31.8725099601594, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1787463, Length=246, Percent_Identity=34.1463414634146, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI87082085, Length=187, Percent_Identity=35.8288770053476, Blast_Score=99, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324266, Length=159, Percent_Identity=35.8490566037736, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24582226, Length=219, Percent_Identity=31.9634703196347, Blast_Score=114, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4; PF05175 MTS [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 31157; Mature: 31157

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLWIEAVRTLSADLEEPFELEFIWRNLHELNKLSWLNLMREKITDQELNLLTELSERLIK
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHC
NEPPQYIVGWAEFYELKFKVDERVLIPRPETEELVEMILTENNNDSLKILDIGTGSGAIA
CCCCCEEEEHHHHEEEEEEECCEEECCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCEEE
ISLAKARQNWSVKASDISQNALELAAENAKMNHVNLEFIQSDVMDELTDRFDIIVSNPPY
EEEEHHCCCCCEEHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCE
IAFDETYEMDDSVIKYEPDLALFAKNQGLAIYQKIADQAVNHLTDDGKIYLEIGYKQGKA
EEECCCCCCCCCEEEECCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHH
VQAIFQEKFTDRLVSIHQDIFGKDRMISVK
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
>Mature Secondary Structure
MLWIEAVRTLSADLEEPFELEFIWRNLHELNKLSWLNLMREKITDQELNLLTELSERLIK
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHC
NEPPQYIVGWAEFYELKFKVDERVLIPRPETEELVEMILTENNNDSLKILDIGTGSGAIA
CCCCCEEEEHHHHEEEEEEECCEEECCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCEEE
ISLAKARQNWSVKASDISQNALELAAENAKMNHVNLEFIQSDVMDELTDRFDIIVSNPPY
EEEEHHCCCCCEEHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCE
IAFDETYEMDDSVIKYEPDLALFAKNQGLAIYQKIADQAVNHLTDDGKIYLEIGYKQGKA
EEECCCCCCCCCEEEECCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHH
VQAIFQEKFTDRLVSIHQDIFGKDRMISVK
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA