Click here to switch to the map view.

The map label for this gene is nfrA1 [H]

Identifier: 125623424

GI number: 125623424

Start: 552428

End: 553183

Strand: Direct

Name: nfrA1 [H]

Synonym: llmg_0559

Alternate gene names: 125623424

Gene position: 552428-553183 (Clockwise)

Preceding gene: 125623423

Following gene: 125623425

Centisome position: 21.84

GC content: 37.83

Gene sequence:

>756_bases
ATGAATGAGAAGAAAATTATGAATCAAACAATTGAAAAAATGTTAGTTCACGCGTCAGTTCGTGATTTTAAAGCTGAACC
TTTGCCAGCTCAAACAAAAGATTTACTGCTAAAAGCAGCACAATCCGGTGCTTCGTCAAATTTTATACAGGCCTATTCAA
TTATTGAGGTAGCGGATGTTAATTTACGACGTGAAATTGCTAAAATTTCTGGTTCGGACGCTTATGTGAATCAAACGGGT
GTATTTTATGTTTTTGTGGCTGATTGGTATCGGCAAGCTCAGATTTTAAGGGCGGAGAATAAAGACGTGAAAGCTCTTCG
TAATATGGAAGCTTTGACAGTTGCCATTGTTGATACTACAATTGCTGCCCAGAATATGACAGTAGCAGCTGAATCTATGG
GGCTTGGCATTTGTTATATTGGTGGAATTCGTAATGACACGTCTAAAGTGGCAGAGTTGTTAAATTTACCAGAATTGACA
CTTCCACTTTTTGGACTGACAATTGGTCTACCAAATAGCCGAAACGAAGTAAAACCACGTTTGCCAAAAGCAAATATTTT
GTCAGTCAATGGTTATAATCATGAATCATTGACTGACTTAAGTACTTATAACGAGCAAACGGCAGAATATTATGCCAATC
GCAGTCATCATCAAAAGTCGACAAATTGGTCACAGGAGATGAGTGATTTTCTTACGAATCCTCGTCGTGAGGACCTGACA
GATTTTTTGAAAAAACAAGGATTTACATTGGACTGA

Upstream 100 bases:

>100_bases
TCTTCTGACGAGAATTTTACCATTGATTTAGAAATAAAAGTAGTCAAAATAGAAGTTAGTTCTAAAATGAAACCTGTCAG
TAGGGAGGAATTTAATTTTG

Downstream 100 bases:

>100_bases
TAAGTTGATAAAGCCTGATTTTATTGGGCTTTTTTGCTATACTTGAAAGAGTAATTTAAAAGAGATTAGAAAAAATAAAA
AGTTTTAGAAAGTGGAAAAG

Product: NADPH-flavin oxidoreductase

Products: NA

Alternate protein names: NADPH-dependent FMN reductase; NADPH-dependent nitroreductase; NADPH-dependent oxidoreductase [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MNEKKIMNQTIEKMLVHASVRDFKAEPLPAQTKDLLLKAAQSGASSNFIQAYSIIEVADVNLRREIAKISGSDAYVNQTG
VFYVFVADWYRQAQILRAENKDVKALRNMEALTVAIVDTTIAAQNMTVAAESMGLGICYIGGIRNDTSKVAELLNLPELT
LPLFGLTIGLPNSRNEVKPRLPKANILSVNGYNHESLTDLSTYNEQTAEYYANRSHHQKSTNWSQEMSDFLTNPRREDLT
DFLKKQGFTLD

Sequences:

>Translated_251_residues
MNEKKIMNQTIEKMLVHASVRDFKAEPLPAQTKDLLLKAAQSGASSNFIQAYSIIEVADVNLRREIAKISGSDAYVNQTG
VFYVFVADWYRQAQILRAENKDVKALRNMEALTVAIVDTTIAAQNMTVAAESMGLGICYIGGIRNDTSKVAELLNLPELT
LPLFGLTIGLPNSRNEVKPRLPKANILSVNGYNHESLTDLSTYNEQTAEYYANRSHHQKSTNWSQEMSDFLTNPRREDLT
DFLKKQGFTLD
>Mature_251_residues
MNEKKIMNQTIEKMLVHASVRDFKAEPLPAQTKDLLLKAAQSGASSNFIQAYSIIEVADVNLRREIAKISGSDAYVNQTG
VFYVFVADWYRQAQILRAENKDVKALRNMEALTVAIVDTTIAAQNMTVAAESMGLGICYIGGIRNDTSKVAELLNLPELT
LPLFGLTIGLPNSRNEVKPRLPKANILSVNGYNHESLTDLSTYNEQTAEYYANRSHHQKSTNWSQEMSDFLTNPRREDLT
DFLKKQGFTLD

Specific function: Reduces FMNH(2) to FMN, with NADPH as reductant. It also reduces nitroaromatic compounds, quinones and azo dyes [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=242, Percent_Identity=38.4297520661157, Blast_Score=164, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 28107; Mature: 28107

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEKKIMNQTIEKMLVHASVRDFKAEPLPAQTKDLLLKAAQSGASSNFIQAYSIIEVADV
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
NLRREIAKISGSDAYVNQTGVFYVFVADWYRQAQILRAENKDVKALRNMEALTVAIVDTT
HHHHHHHHHCCCCCEEECCCEEEEEHHHHHHHHHHHHCCCCHHHHHHCCHHEEEEEEHHH
IAAQNMTVAAESMGLGICYIGGIRNDTSKVAELLNLPELTLPLFGLTIGLPNSRNEVKPR
HHHCCCEEEHHHCCCEEEEECCCCCCHHHHHHHHCCCCHHHHHHHEEECCCCCCCCCCCC
LPKANILSVNGYNHESLTDLSTYNEQTAEYYANRSHHQKSTNWSQEMSDFLTNPRREDLT
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHCCCCHHHHH
DFLKKQGFTLD
HHHHHCCCCCC
>Mature Secondary Structure
MNEKKIMNQTIEKMLVHASVRDFKAEPLPAQTKDLLLKAAQSGASSNFIQAYSIIEVADV
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
NLRREIAKISGSDAYVNQTGVFYVFVADWYRQAQILRAENKDVKALRNMEALTVAIVDTT
HHHHHHHHHCCCCCEEECCCEEEEEHHHHHHHHHHHHCCCCHHHHHHCCHHEEEEEEHHH
IAAQNMTVAAESMGLGICYIGGIRNDTSKVAELLNLPELTLPLFGLTIGLPNSRNEVKPR
HHHCCCEEEHHHCCCEEEEECCCCCCHHHHHHHHCCCCHHHHHHHEEECCCCCCCCCCCC
LPKANILSVNGYNHESLTDLSTYNEQTAEYYANRSHHQKSTNWSQEMSDFLTNPRREDLT
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHCCCCHHHHH
DFLKKQGFTLD
HHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 9836433 [H]