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The map label for this gene is fabI [H]

Identifier: 125623405

GI number: 125623405

Start: 537078

End: 537830

Strand: Direct

Name: fabI [H]

Synonym: llmg_0539

Alternate gene names: 125623405

Gene position: 537078-537830 (Clockwise)

Preceding gene: 125623403

Following gene: 125623412

Centisome position: 21.23

GC content: 40.11

Gene sequence:

>753_bases
ATGTTTTTAGAAGGTAAAAAAATTGTTATCATGGGCGTTGCTAACAATAAATCTATTGCTTGGGGCTGTGCCAAAGCAAT
GAAAGATCAAGGCGCAACATTGATTTATACTTACCAAAACGAACGAATGGAAAAACAATTGGCCAAATTGGCTGAACCAG
AAGATTTGCTCATCGAATGTGATGTTACTTCTGATGAATCTATTCGTCGTGCTTTTGGGACAATCGAAGCGCGTGTTGGT
AAAATTGACGGGCTTGTTCATGCAGTTGCTTACTCTAAAAAAGAAGAGCTTGGTGGAAATGTCACAGATATCTCTCGTGA
TGGTTATGCACTTGCTCAAGATATTTCAGCTTATAGCTTACTTGCAGTTGCTAAAGCAGCGAAACCTTTGCTTAAAAAAG
GTTCTGGTATCGTAACTTTGACTTACATGGGTTCAGTTCGCGCCATTCCTAACTACAATGTTATGGGAATTGCTAAAGCT
GCCCTTGAATCTACTGTTCGCTATCTTGCTGCTGAAATGGCACATGTGGGTGTTCATGTTAACGGAATCTCTGCAGGAGC
AATTAAAACACTTGCTGTTTCAGGAGTTTCAGGTTACAAAGATTTGATTAAAGAATCTGACAGCCGTACAGCTGACGGTG
TTGGTGTGACAATCGAAGAAGTTGGTCAAACAGCTGCCTTCCTTGTTAGCCCACTTGCTTCAGGTGTAATTGGTGATATT
GTTTATGTTGATAAAGGGGTTCATTTAACATAA

Upstream 100 bases:

>100_bases
TTTTTATATGAGTTGCAAAGGTAAAGAGATTAAATTTTTCTTTTTTTATTTATTAGTCGCTGAAACCTTATTATTTTAAA
GAAAAGGACTTATTTTATTT

Downstream 100 bases:

>100_bases
GACAAATATAAAAAATACTGACAGAATTTCTGTCAGTGTTTTTTATATCTTTTACTAGCCAAGAATTAAGAGCCATTGTT
CAAGTTATTTTCATCATTTT

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: Cold shock-induced protein 15; CSI15; NADH-dependent enoyl-ACP reductase; Vegetative protein 241; VEG241 [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MFLEGKKIVIMGVANNKSIAWGCAKAMKDQGATLIYTYQNERMEKQLAKLAEPEDLLIECDVTSDESIRRAFGTIEARVG
KIDGLVHAVAYSKKEELGGNVTDISRDGYALAQDISAYSLLAVAKAAKPLLKKGSGIVTLTYMGSVRAIPNYNVMGIAKA
ALESTVRYLAAEMAHVGVHVNGISAGAIKTLAVSGVSGYKDLIKESDSRTADGVGVTIEEVGQTAAFLVSPLASGVIGDI
VYVDKGVHLT

Sequences:

>Translated_250_residues
MFLEGKKIVIMGVANNKSIAWGCAKAMKDQGATLIYTYQNERMEKQLAKLAEPEDLLIECDVTSDESIRRAFGTIEARVG
KIDGLVHAVAYSKKEELGGNVTDISRDGYALAQDISAYSLLAVAKAAKPLLKKGSGIVTLTYMGSVRAIPNYNVMGIAKA
ALESTVRYLAAEMAHVGVHVNGISAGAIKTLAVSGVSGYKDLIKESDSRTADGVGVTIEEVGQTAAFLVSPLASGVIGDI
VYVDKGVHLT
>Mature_250_residues
MFLEGKKIVIMGVANNKSIAWGCAKAMKDQGATLIYTYQNERMEKQLAKLAEPEDLLIECDVTSDESIRRAFGTIEARVG
KIDGLVHAVAYSKKEELGGNVTDISRDGYALAQDISAYSLLAVAKAAKPLLKKGSGIVTLTYMGSVRAIPNYNVMGIAKA
ALESTVRYLAAEMAHVGVHVNGISAGAIKTLAVSGVSGYKDLIKESDSRTADGVGVTIEEVGQTAAFLVSPLASGVIGDI
VYVDKGVHLT

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Inner Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=251, Percent_Identity=44.2231075697211, Blast_Score=227, Evalue=6e-61,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 26397; Mature: 26397

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLEGKKIVIMGVANNKSIAWGCAKAMKDQGATLIYTYQNERMEKQLAKLAEPEDLLIEC
CCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCCCCEEEEE
DVTSDESIRRAFGTIEARVGKIDGLVHAVAYSKKEELGGNVTDISRDGYALAQDISAYSL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHH
LAVAKAAKPLLKKGSGIVTLTYMGSVRAIPNYNVMGIAKAALESTVRYLAAEMAHVGVHV
HHHHHHHHHHHHCCCCEEEEEEECCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEE
NGISAGAIKTLAVSGVSGYKDLIKESDSRTADGVGVTIEEVGQTAAFLVSPLASGVIGDI
ECCCCCHHHHHEECCCCHHHHHHHCCCCCCCCCCCEEHHHHCCHHHHHHHHHHHHHHHHE
VYVDKGVHLT
EEECCCCCCC
>Mature Secondary Structure
MFLEGKKIVIMGVANNKSIAWGCAKAMKDQGATLIYTYQNERMEKQLAKLAEPEDLLIEC
CCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCCCCEEEEE
DVTSDESIRRAFGTIEARVGKIDGLVHAVAYSKKEELGGNVTDISRDGYALAQDISAYSL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHH
LAVAKAAKPLLKKGSGIVTLTYMGSVRAIPNYNVMGIAKAALESTVRYLAAEMAHVGVHV
HHHHHHHHHHHHCCCCEEEEEEECCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEE
NGISAGAIKTLAVSGVSGYKDLIKESDSRTADGVGVTIEEVGQTAAFLVSPLASGVIGDI
ECCCCCHHHHHEECCCCHHHHHHHCCCCCCCCCCCEEHHHHCCHHHHHHHHHHHHHHHHE
VYVDKGVHLT
EEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377; 8755892; 9298659 [H]