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The map label for this gene is thiD2 [H]

Identifier: 125623330

GI number: 125623330

Start: 457756

End: 458544

Strand: Direct

Name: thiD2 [H]

Synonym: llmg_0463

Alternate gene names: 125623330

Gene position: 457756-458544 (Clockwise)

Preceding gene: 125623329

Following gene: 125623331

Centisome position: 18.1

GC content: 35.61

Gene sequence:

>789_bases
ATGGTAGAGGAATTAGCACAAACAAAAAAAATTCTAACAATTGCAGGTTCAGATATTCTTTCTGGAGGCGGAATGCAAGC
AGATTTAGCCACATTTGCGGCAAATGGCTTATATGGTTTTTGTGCTTTAACAAGTATTGTAACAGTCAAAGAAGACAAGT
TTTTTGTTCACCCTGTAGATGAGGAAATTTTCCAAGAAGAACTTGAAAGTTTGACTGATATTGATTTTTCAGCTATAAAG
ATTGGCTTATTACCTAATAAAAAAATACTAGAGATGACGAAAAAGTGGCTGACAGCTCTGTCAGTAAAATCGCCAGTGCC
TATTATCTTAGATCCGGTTATTGTTTTTAAAGAAAATGCAGACTATTCTGTCAGTGAAATGCGCGAACTATTCATCAGCC
AATTATTTCCACTAGCAACAGTCATTACGCCAAATCTAAAAGAGGCAGAGATTCTAACGGGGCTGTCAGTAAAAACTGAG
GCAGATATGAAAGCAGCAGCTAAACTTTTACATCGTTTTGGTGCCAAAAATGTAATCATAAAAGGCGGAAATCGATTTGA
TCAAAAACAAGCGATTGACTTTGCTTATGATGGGAAAGATTTTTATCAATTAGCAAGTCCGCTTTTACAGAAAAACAACA
ATGGAGCTGGCTGTACATTTGCCTCTTCTATTGCAAGTCAGATGGCTGAGGAAAATTCTTTTAAAGATTCGGTCGAAAAA
GCAAAAGACTTCGTCTACCAAGCTATTCAAAATGCTAATGAATTTGGTGTTTTTCAAAATAAAAAGTAA

Upstream 100 bases:

>100_bases
ACCAACAGCAGCGCCTGAGGGCTTATATCTTAAAGAAGTCAAATATGAACCAATAAATGAAATTTAAAAAATACTTTTAA
CAAAAATTACTGGAGAGAAA

Downstream 100 bases:

>100_bases
ATTTCCAAAAGCAAACCAAACTTCTAGGGAAATTAGGAGATGAAACTCATGGACAATAAAAATATAAAAAAATTAACTTT
ACTTGCGATTTGGACAGCTC

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: PN/PL/PM kinase; Pyridoxal kinase; Pyridoxamine kinase; Vitamin B6 kinase [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MVEELAQTKKILTIAGSDILSGGGMQADLATFAANGLYGFCALTSIVTVKEDKFFVHPVDEEIFQEELESLTDIDFSAIK
IGLLPNKKILEMTKKWLTALSVKSPVPIILDPVIVFKENADYSVSEMRELFISQLFPLATVITPNLKEAEILTGLSVKTE
ADMKAAAKLLHRFGAKNVIIKGGNRFDQKQAIDFAYDGKDFYQLASPLLQKNNNGAGCTFASSIASQMAEENSFKDSVEK
AKDFVYQAIQNANEFGVFQNKK

Sequences:

>Translated_262_residues
MVEELAQTKKILTIAGSDILSGGGMQADLATFAANGLYGFCALTSIVTVKEDKFFVHPVDEEIFQEELESLTDIDFSAIK
IGLLPNKKILEMTKKWLTALSVKSPVPIILDPVIVFKENADYSVSEMRELFISQLFPLATVITPNLKEAEILTGLSVKTE
ADMKAAAKLLHRFGAKNVIIKGGNRFDQKQAIDFAYDGKDFYQLASPLLQKNNNGAGCTFASSIASQMAEENSFKDSVEK
AKDFVYQAIQNANEFGVFQNKK
>Mature_262_residues
MVEELAQTKKILTIAGSDILSGGGMQADLATFAANGLYGFCALTSIVTVKEDKFFVHPVDEEIFQEELESLTDIDFSAIK
IGLLPNKKILEMTKKWLTALSVKSPVPIILDPVIVFKENADYSVSEMRELFISQLFPLATVITPNLKEAEILTGLSVKTE
ADMKAAAKLLHRFGAKNVIIKGGNRFDQKQAIDFAYDGKDFYQLASPLLQKNNNGAGCTFASSIASQMAEENSFKDSVEK
AKDFVYQAIQNANEFGVFQNKK

Specific function: Phosphorylates B6 vitamers; functions in a salvage pathway. Uses pyridoxal, pyridoxine, and pyridoxamine as substrates [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=254, Percent_Identity=29.5275590551181, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1788758, Length=215, Percent_Identity=27.906976744186, Blast_Score=68, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6325378, Length=264, Percent_Identity=28.4090909090909, Blast_Score=89, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6324517, Length=251, Percent_Identity=28.2868525896414, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324997, Length=251, Percent_Identity=27.4900398406374, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.35 [H]

Molecular weight: Translated: 28835; Mature: 28835

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEELAQTKKILTIAGSDILSGGGMQADLATFAANGLYGFCALTSIVTVKEDKFFVHPVD
CCHHHHHHHHEEEEECCHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHEEECCCEEECCCC
EEIFQEELESLTDIDFSAIKIGLLPNKKILEMTKKWLTALSVKSPVPIILDPVIVFKENA
HHHHHHHHHHHHCCCHHHEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEECCEEEEECCC
DYSVSEMRELFISQLFPLATVITPNLKEAEILTGLSVKTEADMKAAAKLLHRFGAKNVII
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEE
KGGNRFDQKQAIDFAYDGKDFYQLASPLLQKNNNGAGCTFASSIASQMAEENSFKDSVEK
ECCCCCCHHHHCCEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHH
AKDFVYQAIQNANEFGVFQNKK
HHHHHHHHHHCCHHCCCCCCCC
>Mature Secondary Structure
MVEELAQTKKILTIAGSDILSGGGMQADLATFAANGLYGFCALTSIVTVKEDKFFVHPVD
CCHHHHHHHHEEEEECCHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHEEECCCEEECCCC
EEIFQEELESLTDIDFSAIKIGLLPNKKILEMTKKWLTALSVKSPVPIILDPVIVFKENA
HHHHHHHHHHHHCCCHHHEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEECCEEEEECCC
DYSVSEMRELFISQLFPLATVITPNLKEAEILTGLSVKTEADMKAAAKLLHRFGAKNVII
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEE
KGGNRFDQKQAIDFAYDGKDFYQLASPLLQKNNNGAGCTFASSIASQMAEENSFKDSVEK
ECCCCCCHHHHCCEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHH
AKDFVYQAIQNANEFGVFQNKK
HHHHHHHHHHCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA