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The map label for this gene is truA [H]

Identifier: 125623329

GI number: 125623329

Start: 456961

End: 457722

Strand: Direct

Name: truA [H]

Synonym: llmg_0462

Alternate gene names: 125623329

Gene position: 456961-457722 (Clockwise)

Preceding gene: 125623328

Following gene: 125623330

Centisome position: 18.07

GC content: 37.14

Gene sequence:

>762_bases
ATGACAAGATACAAAGCCACAATAGCCTATGATGGAACCGATTTTGCAGGTTTTCAAAGCCAAACAAATCAACGCACAGT
TCAAGAAGAAATTGAAAAAGTATTAAGCAAATTAAATTCTTTTGAGCCCGTTATTTTACAAGGCTCTGGCAGAACGGATT
CAGGAGTTCATGCCTTTGGGCAAGTGATTCATTTTGACCTGAATGGCAAAGCGCGTGATTTGGAGCGCTTGCGTTTTGGG
CTAGACACTCAGACACCAGCAGATATTGCGGTAAAAAAAGTAGAACTTGTCCCAGATGATTGGCACGCAAGATACCAAAA
ACATGAAAAAACCTATGAGTATTATTTGGAAAATTCAGTGACTCGTAGTCCTTTTCACAGACATTCTAAAGCTTATTTTC
GTTATCCGCTTAATTTTGAACGGATGCAAGGAGCAATGGTAAAATTAGTTGGTCAACACGATTTTACAGGTTTTACTGCC
TCTGGTTCATCAGTTGATGACAAGGTGCGAACCATTTATCAAGCAGAAATTATTCAACTTGATAAAGAAAATTTCAAATT
TATATTTCGTGGAAATGGTTTTTTGTATAAACAAGTCCGAAATATGGTTGGAACCGTCATTAAAATCGGCAATGACCGAA
TGCCTGTCAGTCAAATTGATAAGATTTTGACCAGCAAAAATAGAAATTTTGCAGGACCAACAGCAGCGCCTGAGGGCTTA
TATCTTAAAGAAGTCAAATATGAACCAATAAATGAAATTTAA

Upstream 100 bases:

>100_bases
ATGCCATTTTTTAGAATTGTCAAAGTCTATATTGAAAAAGGATAAACCATCATAAAAAAAGAAAATAAAGTCGTATGAAA
AATTGTGCTATAATAGAAAA

Downstream 100 bases:

>100_bases
AAAATACTTTTAACAAAAATTACTGGAGAGAAAATGGTAGAGGAATTAGCACAAACAAAAAAAATTCTAACAATTGCAGG
TTCAGATATTCTTTCTGGAG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MTRYKATIAYDGTDFAGFQSQTNQRTVQEEIEKVLSKLNSFEPVILQGSGRTDSGVHAFGQVIHFDLNGKARDLERLRFG
LDTQTPADIAVKKVELVPDDWHARYQKHEKTYEYYLENSVTRSPFHRHSKAYFRYPLNFERMQGAMVKLVGQHDFTGFTA
SGSSVDDKVRTIYQAEIIQLDKENFKFIFRGNGFLYKQVRNMVGTVIKIGNDRMPVSQIDKILTSKNRNFAGPTAAPEGL
YLKEVKYEPINEI

Sequences:

>Translated_253_residues
MTRYKATIAYDGTDFAGFQSQTNQRTVQEEIEKVLSKLNSFEPVILQGSGRTDSGVHAFGQVIHFDLNGKARDLERLRFG
LDTQTPADIAVKKVELVPDDWHARYQKHEKTYEYYLENSVTRSPFHRHSKAYFRYPLNFERMQGAMVKLVGQHDFTGFTA
SGSSVDDKVRTIYQAEIIQLDKENFKFIFRGNGFLYKQVRNMVGTVIKIGNDRMPVSQIDKILTSKNRNFAGPTAAPEGL
YLKEVKYEPINEI
>Mature_252_residues
TRYKATIAYDGTDFAGFQSQTNQRTVQEEIEKVLSKLNSFEPVILQGSGRTDSGVHAFGQVIHFDLNGKARDLERLRFGL
DTQTPADIAVKKVELVPDDWHARYQKHEKTYEYYLENSVTRSPFHRHSKAYFRYPLNFERMQGAMVKLVGQHDFTGFTAS
GSSVDDKVRTIYQAEIIQLDKENFKFIFRGNGFLYKQVRNMVGTVIKIGNDRMPVSQIDKILTSKNRNFAGPTAAPEGLY
LKEVKYEPINEI

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=279, Percent_Identity=32.9749103942652, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI199559785, Length=283, Percent_Identity=29.3286219081272, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1788657, Length=240, Percent_Identity=36.6666666666667, Blast_Score=160, Evalue=7e-41,
Organism=Drosophila melanogaster, GI116007752, Length=304, Percent_Identity=27.9605263157895, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI281362146, Length=264, Percent_Identity=26.5151515151515, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648455, Length=263, Percent_Identity=26.9961977186312, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 29032; Mature: 28901

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRYKATIAYDGTDFAGFQSQTNQRTVQEEIEKVLSKLNSFEPVILQGSGRTDSGVHAFG
CCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHEE
QVIHFDLNGKARDLERLRFGLDTQTPADIAVKKVELVPDDWHARYQKHEKTYEYYLENSV
EEEEEECCCCHHHHHHHHHCCCCCCCHHHEEEEEEECCCHHHHHHHHHHHHHHHHHHCCC
TRSPFHRHSKAYFRYPLNFERMQGAMVKLVGQHDFTGFTASGSSVDDKVRTIYQAEIIQL
CCCHHHHCCCEEEECCCCHHHHHCHHHEEECCCCCCCEECCCCCHHHHHHHHHHHHHHEE
DKENFKFIFRGNGFLYKQVRNMVGTVIKIGNDRMPVSQIDKILTSKNRNFAGPTAAPEGL
CCCCEEEEEECCCCHHHHHHHHHHHHEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
YLKEVKYEPINEI
EEEEECCCCCCCC
>Mature Secondary Structure 
TRYKATIAYDGTDFAGFQSQTNQRTVQEEIEKVLSKLNSFEPVILQGSGRTDSGVHAFG
CCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHEE
QVIHFDLNGKARDLERLRFGLDTQTPADIAVKKVELVPDDWHARYQKHEKTYEYYLENSV
EEEEEECCCCHHHHHHHHHCCCCCCCHHHEEEEEEECCCHHHHHHHHHHHHHHHHHHCCC
TRSPFHRHSKAYFRYPLNFERMQGAMVKLVGQHDFTGFTASGSSVDDKVRTIYQAEIIQL
CCCHHHHCCCEEEECCCCHHHHHCHHHEEECCCCCCCEECCCCCHHHHHHHHHHHHHHEE
DKENFKFIFRGNGFLYKQVRNMVGTVIKIGNDRMPVSQIDKILTSKNRNFAGPTAAPEGL
CCCCEEEEEECCCCHHHHHHHHHHHHEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
YLKEVKYEPINEI
EEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]